Over-Optimistic Projected Future Wheat Yield Potential in the North China Plain: The Role of Future Climate Extremes

Global warming and altered precipitation patterns pose a serious threat to crop production in the North China Plain (NCP). Quantifying the frequency of adverse climate events (e.g., frost, heat and drought) under future climates and assessing how those climatic extreme events would affect yield are important to effectively inform and make science-based adaptation options for agriculture in a changing climate. In this study, we evaluated the effects of heat and frost stress during sensitive phenological stages at four representative sites in the NCP using the APSIM-wheat model. climate data included historical and future climates, the latter being informed by projections from 22 Global Climate Models (GCMs) in the Coupled Model Inter-comparison Project phase 6 (CMIP6) for the period 2031–2060 (2050s). Our results show that current projections of future wheat yield potential in the North China Plain may be overestimated; after more accurately accounting for the effects of frost and heat stress in the model, yield projections for 2031-60 decreased from 31% to 9%. Clustering of common drought-stress seasonal patterns into key groups revealed that moderate drought stress environments are likely to be alleviated in the future, although the frequency of severe drought-stress environments would remain similar (25%) to that occurring under the current climate. We highlight the importance of mechanistically accounting for temperature stress on crop physiology, enabling more robust projections of crop yields under future the burgeoning climate crisis.

Gastric cancer stem cells survive in stress environments via their autophagy system

Cancer stem cells (CSCs) play an important role in the progression of carcinoma and have a high potential for survival in stress environments. However, the mechanisms of survival potential of CSCs have been unclear. The aim of this study was to clarify the significance of autophagy systems of CSCs under stress environments. Four gastric cancer cell line were used. Side population (SP) cells were sorted from the parent cells, as CSC rich cells. The expression of stem cell markers was examined by RT-PCR. The viability of cancer cells under starvation and hypoxia was evaluated. The expression level of the autophagy molecule LC3B-II was examined by western blot. The numbers of autophagosomes and autolysosomes were counted by electron microscope. SP cells of OCUM-12 showed a higher expression of stem cell markers and higher viability in starvation and hypoxia. Western blot and electron microscope examinations indicated that the autophagy was more induced in SP cells than in parent cells. The autophagy inhibitor significantly decreased the viability under the stress environments. These findings suggested that Cancer stem cells of gastric cancer might maintain their viability via the autophagy system. Autophagy inhibitors might be a promising therapeutic agent for gastric cancer.

Time-dependent Seismic Footprint of Thermal Loading for Geothermal Activities in Fractured Carbonate Reservoirs

This paper describes and deploys a workflow to assess the evolution of seismicity associated to injection of cold fluids close to a fault. We employ a coupled numerical thermo-hydro-mechanical simulator to simulate the evolution of pressures, temperatures and stress on the fault. Adopting rate-and-state seismicity theory we assess induced seismicity rates from stressing rates at the fault. Seismicity rates are then used to derive the time-dependent frequency-magnitude distribution of seismic events. We model the seismic response of a fault in a highly fractured and a sparsely fractured carbonate reservoir. Injection of fluids into the reservoir causes cooling of the reservoir, thermal compaction and thermal stresses. The evolution of seismicity during injection is non-stationary: we observe an ongoing increase of the fault area that is critically stressed as the cooling front propagates from the injection well into the reservoir. During later stages, models show the development of an aseismic area surrounded by an expanding ring of high seismicity rates at the edge of the cooling zone. This ring can be related to the “passage” of the cooling front. We show the seismic response of the fault, in terms of the timing of elevated seismicity and seismic moment release, depends on the fracture density, as it affects the temperature decrease in the rock volume and thermo-elastic stress change on the fault. The dense fracture network results in a steeper thermal front which promotes stress arching, and leads to locally and temporarily high Coulomb stressing and seismicity rates. We derive frequency-magnitude distributions and seismic moment release for a low-stress subsurface and a tectonically active area with initially critically stressed faults. The evolution of seismicity in the low-stress environment depends on the dimensions of the fault area that is perturbed by the stress changes. The probability of larger earthquakes and the associated seismic risk are thus reduced in low-stress environments. For both stress environments, the total seismic moment release is largest for the densely spaced fracture network. Also, it occurs at an earlier stage of the injection period: the release is more gradually spread in time and space for the widely spaced fracture network.

In Vitro Flow Chamber Design for the Study of Endothelial Cell (patho)physiology

In the native vasculature, flowing blood produces a frictional force on vessel walls that directly effects endothelial cell phenotype and function. In the arterial system, the vasculature's local geometry directly influences variations in flow profiles and shear stress magnitudes. Straight arterial sections with pulsatile shear stress have been shown to promote an athero-protective endothelial phenotype. Conversely, areas with a more complex geometry, such as arterial bifurcations and branch points with disturbed flow patterns and a lower, oscillatory shear stress, typically lead to endothelial dysfunction and the pathogenesis of cardiovascular diseases. Many studies have investigated the regulation of endothelial responses to various shear stress environments. Importantly, the accurate in vitro simulation of in vivo hemodynamics is critical to the deeper understanding of mechano-transduction through the proper use and design of flow chamber devices. In this review, we describe several flow chamber apparatuses and their fluid mechanics design parameters, including parallel plate flow chambers, cone-and plate devices, and microfluidic devices. In addition, chamber-specific design criteria and relevant equations are defined in detail for the accurate simulation of shear stress environments to study endothelial cell responses

Genome-wide Association Study Revealed Loci Linked to Post-drought Recovery and Traits Related to Persistence of Smooth Bromegrass (Bromus Inermis)

Association analysis has been proved as a powerful tool for genetic dissection of complex traits. This study was conducted to identify marker–trait associations for recovery, persistence, and as well as finding stable associations. In this study, a diverse panel of polycross derived progenies of smooth bromegrass was phenotyped under normal and water stress, during three consecutive years. Association analysis was performed between nine important agronomic traits along with three seasonal growth activity indices based on 535 SRAP markers. Population structure analysis identified five main subpopulations possessing significant genetic differences. Association analysis using mixed linear mode1 identified 339 and 233 marker-trait associations under normal and water stress environments, respectively. Some of these markers were associated with more than one trait; which can be attributed to pleiotropic effects or to a number of tightly linked genes affecting several traits. If the effectiveness of these markers in genetic control of these traits is validated, they could be potentially used for initiation of marker-assisted selection and targeted trait introgression of smooth bromegrass under normal and water stress environments.

Genome-wide association study revealed loci linked to post-drought recovery and traits related to persistence of smooth bromegrass (Bromus inermis)

Association analysis has been proved as a powerful tool for genetic dissection of complex traits. This study was conducted to identify marker – trait associations for recovery, persistence, and as well as finding stable associations. In this study, a diverse panel of polycross derived progenies of smooth bromegrass was phenotyped under normal and water stress, during three consecutive years. Association analysis was performed between nine important agronomic traits along with three seasonal growth activity indices based on 535 SRAP markers. Population structure analysis identified five main subpopulations possessing significant genetic differences. Association analysis using mixed linear mode1 identified 339 and 233 marker-trait associations under normal and water stress environments, respectively. Some of these markers were associated with more than one trait; which can be attributed to pleiotropic effects or to a number of tightly linked genes affecting several traits. If the effectiveness of these markers in genetic control of these traits is validated, they could be potentially used for initiation of marker-assisted selection and targeted trait introgression of smooth bromegrass under normal and water stress environments.