scholarly journals Acaricidal Activity of Crude Ethanolic Extract of Sphaeranthus indicus, its Fractions and Subfractions against Rhipicephalus (Boophilus) annulatus (Acari: Ixodidae)

Author(s):  
Thirumangalath Meethal Divya ◽  
Valliparampil Chandran Soorya ◽  
Krishnan Kavillimakkil Amithamol ◽  
Darsana Udayan ◽  
Kanapadinchareveetil Sreelekha ◽  
...  
Planta Medica ◽  
2020 ◽  
Vol 86 (18) ◽  
pp. 1335-1344
Author(s):  
Darsana Udayan ◽  
Suresh Narayanan Nair ◽  
Sanis Juliet ◽  
Reghu Ravindran ◽  
Sunil Athalathil ◽  
...  

AbstractSince time immemorial, human beings have used various parts of plants in either prevention or treatment of ailments. Plants are rich sources of secondary metabolites such as alkaloids, steroids, terpenoids, flavonoids, and phenolic compounds with a high structural diversity. Many plants/herbs with specific biological activities such as antitumor, antioxidant, anti-inflammatory, antifungal, sedative, and acaricidal activity have been reported. Artemisia nilagirica (C. B. Clarke) Pamp. (Compositae) is a plant traditionally used for insect control in the southern part of India. Previous studies have demonstrated the activity of Artemisia species against pests. The present study thus evaluates the acaricidal activity of crude ethanolic extract of A. nilagirica leaves and its fractions against Rhipicephalus (Boophilus) annulatus. Ticks are ectoparasites that transmit several protozoal, viral, and rickettsial diseases. In south India, R. (B.) annulatus is the commonly observed tick species. Control of these acarine parasites that adversely affect milk and meat production is a tough task. Chemical acaricides such as organophosphates, synthetic pyrethroids, amitraz, and ivermectin are commonly used in tick control. The high cost, environmental hazards, and development of acaricidal resistance are some of the drawbacks of these chemical acaricides. Plant-based formulations are one of the promising approaches for the control of ectoparasites. Previously, extracts from various medicinal/aromatic plants were reported for acaricidal activity from our laboratory, such as Tetrastigma leucostaphylum (Dennst.) Alston, Chassalia curviflora (Wall.) Thwaites, Jatropha curcas L., and Ageratum conyzoides Hieron. Biochemical quantification, fluorescence analysis, and primary phytochemical analysis are already reported for the ethanolic extract and its fractions of areal parts of A. nilagirica. Phytochemical characterization of ethanolic extract of A. nilagirica from Kerala, India was shown to have the presence of terpenoids, flavonoids, steroids, saponins, fixed oils and fats, tannins, and glycosides.


2015 ◽  
Vol 46 (3) ◽  
pp. 536-541 ◽  
Author(s):  
Anne Caroline dos Santos Dantas ◽  
Andreina de Carvalho Araujo ◽  
Alessandra Gomes Marques Pacheco ◽  
Alexsandro Branco ◽  
Luis Antônio Sangioni ◽  
...  

ABSTRACT: This experiment was carried out to study the bioacaricidal activity of Amburana cearensis against engorged females of Rhipicephalus (Boophilus) microplus. The crude ethanolic extract from the leaves of A. cearensis was submitted to partition with organic solvents (hexane, chloroform and ethyl acetate) in order of increasing polarity; and evaluated using three treatments at concentrations of 5, 10 and 25mg ml-1. To evaluate the acaricidal activity, engorged females were submitted to the adult immersion test. Parameters analyzed were the weight of females; weight of egg mass; percentage of hatch; reproductive efficiency; expected rate of reproduction; and efficacy. For each extract and concentrating fraction, tests were performed in triplicate. Among all fractions, the hexane (2.5%) achieved the best results in all parameters analyzed: 52.7% of inhibition of oviposition; 39% of hatching rate; 3,271 index of reproductive efficiency; and 67.0% effectiveness. The extract obtained gradual dose response with increasing concentration; and could be used as an aid in the control of R. (B.) microplus therapy.


2017 ◽  
Vol 69 (3) ◽  
pp. 523-528 ◽  
Author(s):  
A.C.S. Dantas ◽  
D.P. Freire ◽  
G.R. Souza ◽  
J.R.G.S. Almeida ◽  
L.A. Rolim ◽  
...  

ABSTRACT The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.


2014 ◽  
Vol 65 (1) ◽  
pp. 141-148 ◽  
Author(s):  
R. Godara ◽  
S. Parveen ◽  
R. Katoch ◽  
A. Yadav ◽  
M. Katoch ◽  
...  

Author(s):  
Preeja K. Sundaresan ◽  
Kala P. Kesavan

Background: Sphaeranthus indicus Linn is a widely used medicinal plant in Indian traditional system of medicine against human pathogens. Alarming bacterial resistance is urging scientist to search for newer anti-microbial substances from the medicinal plants. The objective of the study was to evaluate the antibacterial activity of ethanolic extract of the whole plant Sphaeranthus indicus Linn (Asteraceae).Methods: The antibacterial activity of ethanolic extract of whole plant of Sphaeranthus indicus Linn was done against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus in Mueller Hinton Agar (MHA) and compared with ciprofloxacin as standard by disc diffusion method.Results: The study revealed that there was no zone of inhibition in doses of 100 mcg, 200 mcg and 300 mcg of ethanolic whole plant extract of Sphaeranthus indicus in MHA plates compared with ciprofloxacin 30 mcg.Conclusions: Ethanolic extract of Sphaeranthus indicus does not have antibacterial activity. Further studies are needed in different extracts and parts of the plant. Simultaneous studies can be done in different places to evaluate environmental factors and regional variations.


2017 ◽  
Vol 5 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Reghu Ravindran ◽  
Sanis Juliet ◽  
Sunil Athalathil Ramankutty ◽  
Nanjundappa Sathish ◽  
Suresh Narayanan Nair ◽  
...  

Author(s):  
Suresh Narayanan Nair ◽  
Sanis Juliet ◽  
Krishnan Kavillimakkil Amithamol ◽  
Athalathil Ramankutty Sunil ◽  
Adarsh Krishna Thumadath Palayullaparambil ◽  
...  

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
S. Ramachandran ◽  
K. Asokkumar ◽  
M. Uma Maheswari ◽  
T. K. Ravi ◽  
A. T. Sivashanmugam ◽  
...  

The present investigation was aimed to study the antidiabetic, antihyperlipidemic, andin vivoantioxidant properties of the root ofSphaeranthus indicusLinn. in streptozotocin- (STZ-) induced type 1 diabetic rats. Administration of ethanolic extract ofSphaeranthus indicusroot (EESIR) 100 and 200 mg/kg to the STZ-induced diabetic rats showed significant () reduction in blood glucose and increase in body weight compared to diabetic control rats. Both the doses of EESIR-treated diabetic rats showed significant () alteration in elevated lipid profile levels than diabetic control rats. The EESIR treatment in diabetic rats produced significant increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and decrease in thiobarbituric acid reactive substances (TBARS) levels than diabetic control rats. Administration of EESIR 200 mg/kg produced significant () higher antioxidant activity than EESIR 100 mg/kg. The high performance liquid chromatography (HPLC) analysis of EESIR revealed the presence of biomarkers gallic acid and quercetin. In conclusion, EESIR possess antidiabetic, antihyperlipidemic, andin vivoantioxidant activity in type 1 diabetic rats. Its antioxidant and lipid lowering effect will help to prevent diabetic complications, and these actions are possibly due to presence of above biomarkers.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
T. P. Adarsh Krishna ◽  
T. P. Ajeesh Krishna ◽  
N. D. Chithra ◽  
P. E. Deepa ◽  
U. Darsana ◽  
...  

The acaricidal activity of the petroleum ether extract of leaves ofTetrastigma leucostaphylum(Dennst.) Alston (family: Vitaceae) againstRhipicephalus (Boophilus) annulatuswas assessed using adult immersion test (AIT). The per cent of adult mortality, inhibition of fecundity, and blocking of hatching of eggs were studied at different concentrations. The extract at 10% concentration showed 88.96% inhibition of fecundity, 58.32% of adult tick mortality, and 50% inhibition of hatching. Peak mortality rate was observed after day 5 of treatment. Mortality of engorged female ticks, inhibition of fecundity, and hatching of eggs were concentration dependent. The LC50value of the extract againstR. (B.) annulatuswas 10.46%. The HPTLC profiling of the petroleum ether extract revealed the presence of at least seven polyvalent components. In the petroleum ether extract, nicotine was identified as one of the components up to a concentration of 5.4%. However, nicotine did not reveal any acaricidal activity up to 20000 ppm (2%). Coconut oil, used as diluent for dissolving the extract, did not reveal any acaricidal effects. The results are indicative of the involvement of synergistic or additive action of the bioactive components in the tick mortality and inhibition of the oviposition.


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