scholarly journals Identification of Cryptosporidium sp. with the Microscopic Examination Method on HIV/AIDS Patients in Padang, West Sumatra

Author(s):  
Nuzulia Irawati Nora Harminarti ◽  
A. K. Munzi Yulianto Kemala Sayuti

Cryptosporidiosis is a zoonosis disease which classified as waterborne disease caused by Cryptosporidium sp. and can inflict acute to chronic diarrhea through small intestine infection. The basic in diagnosing cryptosporodiosis is microscopic or moleculer inspection of feces samples. The objective of this study was to find out the Cryptosporidium sp. infection in HIV/AIDS patients with cluster of differentiation 4 (CD4) ≤ 350 sel/mm3 in m djamil padang. This study was observational descriptive research by using feces, questionnaires, and medical record. This research samples were part of the population which fulfill in the inclusion and exclusion criteria in VCT Policlinic of m djamil padang. Two from forty respondences of this study was found positive of Cryptosporidium sp. Oocyst in their feces. The frequencies based on gender could not be determined because the different number of men and women respondences. From the anamnesis by using questionnaires, this two respondences with positive result did not have diarrhea (asymptomatic). Based on data on drinking water sources and daily water sources as a risk for Cryptosporidium sp infection, there were no significant numbers. From the same typed of water sources, more was found to be negative oocyst than positive oocyst. Based on the source of their drinking water, one of the used cooked water and the other one used retail refill water. Based on their daily water resources, both of them using Water Supply Company (PDAM). It could be influenced by the location, environmental factors, and activities of each of them those were different.

2019 ◽  
Author(s):  
C.M.N Khabo-Mmekoa ◽  
M.N.B Momba

ABSTRACTThis aim of this study was to ascertain whether household container-stored drinking water might play a role in the diarrhoeic conditions of HIV/AIDS patients and non-HIV-infected individuals of the rural communities who attended the Ugu District Municipal hospitals. Water samples were collected from the standpipes and household containers, and stool specimens were obtained from HIV/AIDS-positive and non-HIV/AIDS patients with diarrhoea. Significant correlations were established between the incidence of potentially pathogenic bacteria isolated from chlorinated household-stored water, and in stool specimens of HIV-positive patients with diarrhoea (r = P < 0.05). A combination of molecular analysis targeting the 16S rRNA gene and the restriction fragment length polymorphism and sequence analysis of the amplified gene for differentiating between species and strains of the bacterial pathogens was also applied to isolates obtained from stored-water samples and stool specimens. Similar sequences of Klebsiella spp., K. pneumoniae, Escherichia coli, E. coli O55: H7, Proteus mirabilis, and Shigella boydii were identified in both stored water and stools of HIV/AIDS-positive patients with diarrhoea. With the exception of Proteus mirabilis, none of these pathogens were identified in stool specimens of non-HIV-infected individuals with diarrhoea.


2020 ◽  
Vol 10 (4-s) ◽  
pp. 197-200
Author(s):  
Filia Yuniza ◽  
Eddy Mart Salim ◽  
Nova Kurniati ◽  
Harun Hudari ◽  
Erial Bahar ◽  
...  

Aims: to determine the effect of NAC administration on hematological abnormalities in HIV/AIDS patients who are undergoing ARV treatment. Method: This was a pilot study using a double blind randomized clinical trial. A total of 32 subjects who met the inclusion and exclusion criteria were divided into 2 groups, namely placebo and NAC. Subjects in the NAC group were given NAC at a dose of 3x200 mg/day, while the placebo group was given lactose at a dose of 3x200 mg/day. Each group was given treatment for 12 weeks. Before and after treatment, subjects were examined for hematologic parameters. Results:  Most of subjects had normal hematological features. NAC administration did not have a significant effect on the mean levels of Hb, Ht, number of erythrocytes, leukocytes and platelets (p> 0.05). However, NAC administration can reduce the degree of anemia and improve the condition of thrombocytopenia, leukopenia and lymphopenia. Conclusion: NAC administration can reduce hematological abnormalities HIV/AIDS patients undergoing ARV treatment. Keywords: ARV, Hematological abnormalities, HIV/AIDS, NAC.


Konselor ◽  
2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Surahma Wahyu ◽  
Taufik Taufik ◽  
Asmidir Ilyas

Abstrak this research early from fact that growing of patient of HIV aids specially in West Sumatra. Natural Patient of dread, stress, depresi, soul convulsion, and discrimination of stigmatisasi. Here in after the situation will pursue development of self concept of odha positively causing low feeling of self, feel worthless self and show wrong behaviour. This research is descriptive research with aim to express self concept and natural by dominant problems of odha.


2018 ◽  
Vol 12 (1) ◽  
pp. 106-116
Author(s):  
Yeboah K. Opoku ◽  
Johnson N. Boampong ◽  
Irene Ayi ◽  
Godwin Kwakye-Nuako ◽  
Dorcas Obiri-Yeboah ◽  
...  

Objective:To identify the socio-behavioral risk factors associated with cryptosporidiosis among HIV/AIDS patients with chronic diarrhea symptoms visiting the HIV referral clinic at Cape Coast Teaching Hospital, Ghana.Methods:A cross-sectional study was conducted among 50 HIV/AIDS patients with recurrent diarrhea. Questionnaires were administered to collect social and behavioral risk factors associated withCryptosporidiumand other opportunistic protozoan parasitic infections in HIV patients. Stool samples were collected for the diagnosis of enteric protozoan pathogens using modified Ziehl-Neelsen and acid-fast staining methods. CD4+cells counts of study subjects were obtained from patients clinical records. The data obtained were analyzed using Pearson chi-square and multivariate-adjusted statistics tool on SPSS 16 for Windows.Results:Twenty-seven (54%) of the subjects were infected with enteric protozoan pathogens. The prevalences ofCryptosporidium,CyclosporaandMicrosporidiuminfections were 46%, 32% and 16%, respectively.Cryptosporidiuminfection was significantly associated with drinking water (×2=13.528, p<0.001),Cyclosporawas associated with the type of drinking water (×2=14.931, p<0.001) and toilet facilities used by the study subjects (×2=12.463, p<0.01), whilesMicrosporidiuminfection was associated with hand washing behavior (×2=12.463, p<0.01). Enteric protozoans were frequently encountered among subjects with CD4+ T-cell count <200 cells/mm3. However, coinfection ofCyclospora spp&Cryptosporidiumspp was not observed in CD4+cell count <200 and >500 cells/mm3.Multivariate analysis showed that the risk factor forCryptosporidiuminfection among HIV/AIDS patients was the source of drinking water (pipe borne water 76.2% prevalence: sachet water 25%; OR=0.10, 95%CI: 0.03-0.39, p<0.001).Conclusion:We report the risk factor for exposure ofCryptosporidiuminfection among HIV/AIDS patients for the first time in Ghana. The contamination of drinking water by protozoan parasites should be a public health concern. These results provide the stepping block to understand the transmission dynamics ofCryptosporidiumand other opportunistic pathogens in HIV/AIDS infected patients in Ghana.


2020 ◽  
Author(s):  
Mulualem Kiros ◽  
Mucheye Gizachew ◽  
Teklay Gebrecherkos

Abstract Background: Globally, two-thirds of humans are infected with Helicobacter pylori (H. pylori), a flagellated Gram negative bacterium, brings peptic ulcer disease and others, mainly, in HIV patients on ART. Its infection among HIV/AIDS patients is not well understood in developing countries, including the study area.Objective: To assess prevalence of H. pylori infection and associated risk factors among HIV/AIDS on ART patients at Tefera Hailu General Memorial hospital Sekota Northeast, Ethiopia. Methods: An institution based cross sectional study was conducted on HIV/AIDS on ART patients from February to June 2019. A face-to-face interview was administered to assess risk factors for H. pylori infection by using semi structured questionnaire. A stool sample was tested for H. pylori antigen using the ImmunoCardSTAT HpSA kit as per the manufactures` instruction. Data were entered into excel spreadsheet, cleaned, exported to and analyzed by using the SPSS version 20. Logistic regression model was used to determine the association between explanatory and outcome variables. For all cases, p-value < 0.05 was considered statistically significant. Results: A total of 88 HIV/AIDS patients on ART were included in this study. Of these, 53.4% were females, 54.5% were urban dwellers, 69.3% were single, 51.1% had formal education and 58.0% were non-employed. From these, study participants, a total of 88 stool samples were collected and analyzed, and 57 (64.8%, 95% CI: 54.5-73.9) H. pylori positivity was observed. Among explanatory variables tested, number of family members (AOR: 17.059; 95%CI: 1.751, 166.185; p-value = 0.015) and water sources for drinking and cooking (AOR: 0.096; 95%CI: 0.010, 0.951; p-value = 0.045) were statistically significant with H pylori positivity. Other factors did not show association with H. pylori infection (p-value > 0.05). Conclusion: Prevalence of H. pylori among HIV on ART patients was within the ranges of global prevalence (10%-76%). Number of family members and water sources for drinking and cooking purpose were found to be factors associated with the H. pylori positivity in this study. This finding necessitates the need to design and apply intervention measures that could decrease transmission and thus minimize the clinical consequences of infection.


el–Hayah ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 28-35
Author(s):  
Muhamad Syamsussabri ◽  
Riza Nurhermi Ningtyas ◽  
Amalia Ainun Najah ◽  
M. Saiful Fahmi ◽  
Endang Suarsini

This study aims to determine the contamination of coliform bacteria in drinking water sources of residents in Malang City. Type of this research is explorative descriptive research. The study population was all drinking water sources of residents throughout Malang City, while the research sample was 15 residents wells in five subdistricts of Malang City with each sample taken three sample points. The samples were tested using 3M petrifilm E. coli/coliform count plate. The results showed that all the samples studied were contaminated with coliform bacteria with the highest percentage of 23.01% for E. coli bacteria contamination and 15.41% for total coliform bacterial contamination with an average of bacterial colonies 200 colonies.


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