scholarly journals Analysis of Coliform Bacteria Contamination in Drinking Water Sources in Malang City

el–Hayah ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 28-35
Author(s):  
Muhamad Syamsussabri ◽  
Riza Nurhermi Ningtyas ◽  
Amalia Ainun Najah ◽  
M. Saiful Fahmi ◽  
Endang Suarsini

This study aims to determine the contamination of coliform bacteria in drinking water sources of residents in Malang City. Type of this research is explorative descriptive research. The study population was all drinking water sources of residents throughout Malang City, while the research sample was 15 residents wells in five subdistricts of Malang City with each sample taken three sample points. The samples were tested using 3M petrifilm E. coli/coliform count plate. The results showed that all the samples studied were contaminated with coliform bacteria with the highest percentage of 23.01% for E. coli bacteria contamination and 15.41% for total coliform bacterial contamination with an average of bacterial colonies 200 colonies.

2018 ◽  
Vol 19 (1) ◽  
pp. 128-136 ◽  
Author(s):  
S. Taonameso ◽  
L. S. Mudau ◽  
A. N. Traoré ◽  
N. Potgieter

Abstract Sporadic outbreaks of diarrhoea in children in the Vhembe rural areas could be an indication of contamination in drinking water sources. In areas where improved water sources are used, not all rural households experience the benefits of these improved water sources. Water samples were collected from boreholes in three wards in the Vhembe District to determine microbiological risks over a 5-month period. A Water Point Mapping tool was used to indicate the borehole distribution. Water samples were taken from each functional borehole and analysed for total coliform and Escherichia coli counts, electrical conductivity, pH and temperature. A multiplex PCR protocol was used for identification of pathogenic E. coli. A total of 125 boreholes were identified of which only 12 were functional. Seven boreholes tested positive for total coliforms and E. coli counts. Four boreholes (33.3%) tested positive for diarrhoeagenic E. coli. Fifty-eight percent (58%) of water samples were without health risks, 17% were low risk and 25% could cause infection according to the South African water quality standards. This study indicated the importance of the role of the Municipalities and the maintenance plans that need to ensure that all boreholes are functional and provide safe drinking water to the rural communities.


Author(s):  
Sabah Shareef Mohammed ◽  
Yadgar Hussien Hama-karim ◽  
Mohammed Ibrahim Mohialdeen Gubari

Water pollution in Iraq has been extended disaster edge; this pollution is caused by wastes and sewages into soil and rivers, pollutant water sources influence the outbreak and serious epidemic status among the population.. The aim of this study is to determine the bacteriological quality of drinking water sources and characteristics of water-borne diseases especially diarrheal cases in Darbandikhan city. 166 water samples, collected from the different sources and areas, were tested for the presences of coliform bacteria as an indicator for pathogen contamination. Most probable number index was used for coliform enumeration. 161 diarrheal cases were taken as a sample from the patients were admitted to the general hospital in Darbandikhan district.. The questionnaire form was planned to view characteristics of diarrheal cases and patients were interviewed directly, the data was analyzed by STATA software application. 46% of the diarrheal cases used tap water for drinking. On the other hand, the reminder (54%) used other sources for the same purpose. All the risk factor such as type of water source, sufficiency of the water, duration of water storage and chlorination were associated with diarrhea. Almost half of the cases were children and three quarters were single. The data was analyzed by STATA version 13.1.This study indicated that the majority of drinking water sources in Darbandikhan city are not suitable for drinking, although net pipe system supplied chlorine significantly it has been proven that the tap water from the homes is not suitable for consumption.


Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte D. Smith

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Guadalajara Metropolitan Area is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and E. coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.


Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte Smith

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Metropolitan Zone of Guadalajara is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and Escherichia coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.


2016 ◽  
Vol 2 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Narayan Dutt Pant ◽  
Nimesh Poudyal ◽  
Shyamal Kumar Bhattacharya

BACKGROUND: Nepal is one of the least developed countries and in most of the areas the water supplied directly from natural water sources (like streams, rivers etc.) is used for drinking purpose, without any prior treatment. So every year there are many water related outbreaks mainly in rainy season. Very little data is available about the bacteriological quality of the water from drinking water sources, in the country. This report presents a scenario of the bacteriological quality of the water from drinking water sources and reservoirs, supplying Dharan municipality of Nepal.METHODS: Bacteriological analysis of the water samples was performed by membrane filter technique and spread plate technique. Total coliform count (TCC), fecal coliform count (FCC) and fecal streptococcal count (FSC) were performed by using membrane filter technique and total plate count (TPC) was performed by spread plate method.RESULTS: Most of the sources and reservoir tanks were found to be heavily contaminated with heterotrophic bacteria, total coliforms, fecal coliforms and fecal streptococci. Contamination with more than one types of indicator organisms was common.CONCLUSIONS: Most of the sources and reservoirs were found to be heavily contaminated with indicator organisms suggesting the alarming situation of water pollution in the area. Abrupt action is needed to be taken to improve the bacteriological quality of the water sources and reservoirs, supplying drinking water to Dharan municipality of Nepal. 


2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.


Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte Smith

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Guadalajara Metropolitan Area is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and E. coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.


Author(s):  
B. E. Agbo ◽  
A. V. Ogar ◽  
U. L. Akpan ◽  
C. I. Mboto

This study evaluated the physico-chemical and the bacteriological quality of five different sources of drinking water in Calabar metropolis, Nigeria, to give a fair geographical representative of the town and to contribute to our understanding of the quality of drinking water in the metropolis. The physico-chemical characteristics such as pH, temperature, turbidity, conductivity, colour, Iron, dissolved oxygen, Calcium, Magnesium, alkalinity, total hardness, Manganese, Sulphate, Chloride, Phosphate, Sodium, Zinc, Copper, total dissolved solid, Nitrate, Nitrite, Ammonia, Ammonium and Potassium were determined following the procedures prescribed by American Public Health Association Standard Method. The bacteriological analysis was carried out using the standard microbiological standard for analysis of water for total and faecal coliform count. The mean temperature of the evaluated waters ranged from 23.03°C-29.3°C, mean pH ranged from 4.37-6.76, while turbidity had a mean range of 0.16 NTU-4.13 NTU. Conductivity ranged between 39.29 µs/cm - 120.7 µs/cm, dissolved oxygen with 13.30 mg/L - 4.19 mg/L, total dissolved solids ranged from 72.4 mg/L -23.5 mg/L, while the mean for iron concentration ranged from 0.12 mg/L - 0.99 mg/L. Similarly, the mean for total hardness was 34.2 mg/L - 17.1 mg/L and 7.93 mg/L - 6.71 mg/L for total alkalinity. Others includes Manganese (0.88 mg/L - 0.02 mg/L), Magnesium (16.5 mg/L - 9.9 mg/L), Calcium (9.77 mg/L - 7.20 mg/L, Nitrate (14.6 mg/L - 3.66 mg/L), Nitrite (0.076 mg/L -0.009 mg/L), Ammonia (0.89 mg/L - 0.25 mg/L), Ammonium (0.52 mg/L - 0.013 mg/L), Zinc (1.01 mg/L - 0.34 mg/L), Chloride (5.73 mg/L - 0.364 mg/L), Fluoride (0.76 mg/L - 0.277 mg/L), Copper (0.61 mg/L - 0.18 mg/L), Sodium (2.73 mg/L - 0.180 mg/L), potassium (5.73 mg/L - 2.0 mg/L), Sulphate (14.8 mg/L - 3.69 mg/L and Phosphate with 4.8 mg/L - 3.69 mg/L. The total coliform count for bottled water ranged between 2.00 cfu/100 mL – 19.00 cfu/100 mL, the total coliform range for sachet water were 6.00 cfu/100 mL and 15.00 cfu/100 mL and no faecal coliform was detected. Public water had no growth at all, the stream and borehole bacteriological analysis ranged from 27x101 cfu/ mL - 55x101 cfu/mL and 12 cfu/100 mL - 33 cfu/100 mL for total coliform respectively. Faecal coliform ranged from 15x101 cfu/ mL - 52x101 cfu/ mL for stream and 9.00 cfu100/ mL – 16.00 cfu/100 mL for borehole. A total of seven (7) different bacteria species were isolated from the sampled drinking water sources. These included Proteus spp., Streptococcus spp., Enterobacter spp., Pseudomonas spp., E.coli, Chromobacter spp., Salmonella spp. and Enterococcus spp. This study reveals a high level of poor quality sources of water in the metropolis and makes need for urgent health intervention.


Author(s):  
A. A. Okwelle ◽  
B. E. Asikong ◽  
J. A. Lennox

Background: Majority of the human population in semi-urban and urban areas in Nigeria are heavily reliant on well and stream water as the main source of water supply for drinking and domestic use due to the inadequate provision of potable pipe borne water. These groundwater sources can easily be fecally contaminated and thus, increase the incidence and outbreaks of preventable waterborne diseases. This study was carried out to determine the bacteriological quality of some well and stream waters sources in Ikwerre, Emohua and Etche Local Government Areas of Rivers State. Methodology: Twenty-four sampling sites with 8 from each Local Government Area were randomly selected for this study. A total of 48 samples, 2 from each of the sites were collected aseptically for 12 months. Samples were analysed using membrane filtration technique for total heterotrophic bacterial count and total coliform count. The well and stream water samples were also cultured for E. coli, Salmonella, and Vibrio sp. Results: The results show that Emohua Local Government Area had the highest total heterotrophic bacterial count of 5.2 x 103 cfu/ml. This was followed by Ikwerre LGA with total bacterial count of 4.3 x 102 cfu/ml, while Etche LGA had 3.1 x 102 cfu/ml. The total coliform count ranged from 25 cfu/100ml to 50 cfu/100ml for the three LGA’s, whereas the faecal coliform count was between 12 cfu/100ml and 20 cfu/100ml. The total coliform, E. coli, Salmonella and Vibrio counts from the three local government areas occurred above the WHO stipulated zero count in 100ml of water. Conclusion: The results from this study showed contamination of all the wells studied with faecal coliforms thus, indicating the presence of other enteric pathogens and a potential source for waterborne disease outbreaks. Well water in Ikwerre, Emohua and Etche Local Government Areas of Rivers State are not safe for drinking without additional treatment like disinfection or boiling. Periodic testing and constant monitoring of these water sources should also be done to meet up with the World Health Organization Standards in the provision of safe, clean drinking water. 


Sign in / Sign up

Export Citation Format

Share Document