scholarly journals Elicitation of Flavonoids in Blumea lacera (Burm.f.) DC. Cell Culture using Chemical Elicitor, Salicylic Acid and Biological Elicitor, Aspergillus niger

2016 ◽  
Vol 3 (11) ◽  
pp. 85-91
Author(s):  
Vijay D. Mendhulkar ◽  
Priyanka Patade ◽  
Moinuddin Vakil
Keyword(s):  
Cell Culture ◽  
Salicylic Acid ◽  
Aspergillus Niger ◽  
Blumea Lacera

scholarly journals Preliminary phytochemical screenings and Pharmacological activities of Blumea lacera (Burn.f.) DC.

2015 ◽  
Vol 13 (1) ◽  
pp. 69-73
Author(s):  
Md Abul Khair ◽  
Mohammed Ibrahim ◽  
Qamrul Ahsan ◽  
Md Ruhul Kuddus ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Crude Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Acetyl Salicylic Acid ◽  
Antipyretic Activity ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Blumea Lacera

The methanol extract of the whole plant of Blumea lacera (Burn.f.) DC. (BLME) has been subjected to preliminary screenings for phytoconstituents and antipyretic, analgesic and anti-inflammatory activities. Antipyretic activity was assessed by the yeast-induced hyperthermia in mice. The analgesic property was evaluated by formalin-induced writhing test. Acetyl salicylic acid (ASA) was used as standard for in-vitro anti-inflammatory activity test. In yeast-induced pyrexia, the crude extract demonstrated a significant (p=0.05) reduction in body temperature of mice after elevation by the administration of yeast. These effects were pronounced at the 2nd and 3rd h of post-treatment with the extract. BLME exhibited a dose-dependent analgesic activity with 39.13% and 56.52% protection at 200-and 400-mg/kg, b.w., respectively as compared to 76.09% revealed by the standard diclofenac sodium. In the anti-inflammatory test, the crude extract at 400 ?g/ml displayed 62.40% inhibition of protein denaturation whereas standard acetyl salicylic acid exhibited 76.74% inhibition. Results of the preliminary phytochemical screenings demonstrated the presence of alkaloids, flavonoids and triterpenoids in the extract. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21863 Dhaka Univ. J. Pharm. Sci. 13(1): 69-73, 2014 (June)

Copper (II) Accumulation And Superoxide Dismutase Activity During Growth Of Aspergillus Niger B-77

2002 ◽  
Vol 57 (3-4) ◽  
pp. 319-322 ◽  
Author(s):  
Kolishka Tsekova ◽  
Dessislava Todorova
Keyword(s):  
Heavy Metal ◽  
Cell Culture ◽  
Superoxide Dismutase ◽  
Aspergillus Niger ◽  
Heavy Metal Ions ◽  
Microbial Growth ◽  
Biological Activities ◽  
Sod Activity ◽  
Growing Cell ◽  
A Cell

The influence of copper (II) ions on the growth, accumulation properties and superoxide dismutase (SOD) activity of a growing culture of Aspergillus niger B-77 were studied. Microbial growth, the level of copper (II) accumulation and SOD activity depended on the initial copper (II) concentration. Aspergillus niger is able to accumulate large amounts of copper (II) from the nutrient medium with 200 mg.l-1 copper (II) ions without loosing its biological activities. Addition of copper (II) ions increased the SOD activity in the growing cell cultures. The changes in enzyme activity induced by heavy metal ions might be used as an indicator of intracellular oxy-intermediate generation in a cell culture growing under stress conditions

scholarly journals Electropolymerised pH Insensitive Salicylic Acid Reference Systems: Utilization in a Novel pH Sensor for Food and Environmental Monitoring

Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 555
Author(s):  
Monica Miranda Mugica ◽  
Kay Louise McGuinness ◽  
Nathan Scott Lawrence
Keyword(s):  
Cell Culture ◽  
Salicylic Acid ◽  
Reference System ◽  
Culture Media ◽  
Ph Sensor ◽  
Active Species ◽  
Ph Sensing ◽  
Sensing Element ◽  
Electrochemical Response ◽  
Local Ph

This work summarizes the electrochemical response of a salicylic acid-based carbon electrode for use as a novel solid-state reference electrode in a redox-based pH sensor. This novel reference produces a pH insensitive response over a range of pH 3–10 in solutions with low buffer concentrations, different compositions, conductivities, and ionic strengths is produced. The pH of the local environment is shown to be determined by the chemistry and the electrochemical response of the redox active species on the surface of the electrode; the local pH can be controlled by the electropolymerized salicylic acid moieties due to the acid concentration on the surface, avoiding any perturbation in environmental pH and leading to a stable novel reference system. Sensitivities of −7.1 mV/pH unit, −2.4 mV/pH unit, −0.2 mV/pH unit, and 2.5 mV/pH units were obtained for different food medias, hydroponic solution, seawater, and cell-culture media, respectively, confirming its ability to control the local pH of the electrode. This reference system is paired with a new pH sensing element based on electropolymerized flavanone to provide a calibration free, pH sensitive sensor to effectively and accurately measure the pH of various media with high viscosity, low conductivity, low/high buffer concentration or cell-culture environment, presenting a maximum error of +/−0.03 pH units.

scholarly journals Discovery and functional analysis of a salicylic acid hydroxylase from Aspergillus niger

2021 ◽  
Author(s):  
Ronnie J.M. Lubbers ◽  
Adiphol Dilokpimol ◽  
Jaap Visser ◽  
Kristiina S. Hildén ◽  
Miia R. Mäkelä ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Aspergillus Niger ◽  
Metabolic Pathways ◽  
Biochemical Characterization ◽  
Plant Biomass ◽  
Pathogenic Fungi ◽  
Alternative Methods ◽  
Muconic Acid

Salicylic acid plays an important role in the plant immune response and its degradation is therefore important for plant pathogenic fungi. However, many non-pathogenic microorganisms can also degrade salicylic acid. In the filamentous fungi Aspergillus niger, two salicylic acid metabolic pathways have been suggested. The first pathway converts salicylic acid to catechol by a salicylate hydroxylase (ShyA). In the second pathway, salicylic acid is 3-hydroxylated to 2,3-dihydroxybenzoic acid followed by the decarboxylation to catechol by 2,3-dihydroxybenzoate decarboxylase (DhbA). A. niger cleaves the aromatic ring of catechol catalyzed by catechol 1,2-dioxygenase (CrcA) to form cis,cis-muconic acid. However, the identification and role of the genes and characterization of the enzymes involved in these pathways are lacking. In this study, we used transcriptome data of A. niger grown on salicylic acid to identify genes (shyA and crcA) involved in salicylic acid metabolism. Heterologous production in Escherichia coli followed by biochemical characterization confirmed the function of ShyA and CrcA. The combination of ShyA and CrcA demonstrated that cis,cis-muconic acid can be produced from salicylic acid. In addition, the in vivo roles of shyA, dhbA and crcA were studied by creating A. niger deletion mutants which revealed the role of these genes in the fungal metabolism of salicylic acid. Importance Nonrenewable petroleum sources are depleting and therefore alternative sources are needed. Plant biomass is one of the most abundant renewable sources on earth and is efficiently degraded by fungi. In order to utilize plant biomass efficiently, knowledge about the fungal metabolic pathways and the genes and enzymes involved is essential to create efficient strategies for producing valuable compounds such as cis,cis-muconic acid. cis,cis-muconic acid is an important platform chemical that is used to synthesize nylon, polyethylene terephthalate (PET), polyurethane, resins and lubricants. Currently, cis,cis-muconic acid is mainly produced through chemical synthesis from petroleum based chemicals. Here, we show that two enzymes from fungi can be used to produce cis,cis-muconic acid from salicylic acid and contributes 40 in creating alternative methods for the production of platform chemicals.

Preparation and research of antibacterial composite materials based on ABS and oligethylene ether of salicylic acid

2020 ◽  
pp. 59-64
Author(s):  
N. Sh. Rasulzade ◽  
◽  
L. Sh. Abbasova ◽  
B. A. Mamedov ◽  
P. Z. Muradov ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Composite Materials ◽  
Aspergillus Niger ◽  
Fusarium Moniliforme ◽  
Antibacterial Properties ◽  
Physicomechanical Properties ◽  
Thermophysical Parameters ◽  
Relative Improvement ◽  
Test Culture

To give antibacterial properties to ABS-based composite materials, oligoethylene ester of salicylic acid in an amount of 0.5 – 1.0 % was used as additive. It was found that additive practically do not affect the physicomechanical properties of composite materials, but lead to a relative improvement in thermophysical parameters and the appearance of antibacterial properties in them. The antibacterial properties were studied and the possibilities of application of the obtained composite materials were determined. Micromycetes such as Aspergillus niger, A.ochraseus, Penicillium cuclopium, Cladosporium herbarium, Fusarium moniliforme and F.oxysporium were used as a test culture. It was found that the studied composite materials have both bactericidal and fungicidal properties. The fungicidal properties of the obtained new antibacterial composite materials allow their use in the manufacture of products resistant to fungal influence.

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