scholarly journals Indigofera zollingeriana Leaf Extract Reduces Sheep Rumen Methane Production in Vitro

2020 ◽  
Vol 22 (1) ◽  
pp. 16-23
Author(s):  
FM Suhartati
Keyword(s):  
Methane Production ◽  
Leaf Extract ◽  
Rumen Fluid ◽  
Gas Production ◽  
Bacterial Populations ◽  
Methane Gas ◽  
Randomized Design ◽  
Indigofera Zollingeriana ◽  
Sheep Rumen

An experimental study aimed at assessing the reduction of gas methane production in   thin-tailed sheep through the use of Indigofera zollingeriana leaf extract was conducted from May to September 2019 at Animal Nutrition and Feed Laboratory, University of Jenderal Soedirman Purwokerto. A One Way Classification of Completely Randomized Design (CRD) experiment was employed. The treatment included Indigofera zollingeriana leaf extract with levels of 0%, 0.40%, and 0.80% of feed dry matter and each treatment was repeated six times so that there were 18 experimental units. The feed provided consisted of 60% concentrate and 40% ammoniated rice straw. The concentrate consisted of coconut cake and rice bran with a ratio composition of 1:2. The material used was rumen fluid obtained from three thin-tailed sheep, sampled from the Sokaraja slaughterhouse immediately after the sheep were slaughtered. In vitro incubation was carried out for four hours. The variables measured included protozoan population, methane gas production and bacterial populations. The data obtained were then analyzed using analysis of variance and continued with the Orthogonal Polynomial test. The use of 0.80% Indigofera zollingeriana leaf extract can reduce the protozoan populations, methane gas production and can increase the bacterial populations of sheep rumen fluid.

scholarly journals In vitro fermentation, digestibility and methane production of tropical perennial grass species

2014 ◽  
Vol 65 (5) ◽  
pp. 479 ◽  
Author(s):  
Belete Shenkute Gemeda ◽  
Abubeker Hassen
Keyword(s):  
Organic Matter ◽  
Methane Production ◽  
Rumen Fluid ◽  
Perennial Grass ◽  
Gas Production ◽  
Automated System ◽  
Grass Species ◽  
Tropical Conditions ◽  
Acid Detergent Fibre

This study characterised 16 tropical perennial grass species in terms of in vitro methane output and related their digestibility and rumen fermentation with methane output. The grass samples were collected, dried in a forced oven, and ground and analysed for nutrient composition. In vitro gas production and organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure gas production through in vitro incubation at 39°C. Anthephora argentea and Stipagrostis ciliate produced the highest concentration of methane in terms of g kg–1 digestible dry matter (DDM) and g kg–1 digestible organic matter (IVOMD). Cenchrus ciliaris, Setaria verticillata and Panicum coloratum produced the lowest (P < 0.05) methane when expressed in terms of g kg–1 DDM and g kg–1 IVOMD. Ash, ether extract, non-fibrous carbohydrate, neutral and acid detergent insoluble nitrogen, and crude protein were negatively correlated with methane production. Methane production positively correlated with neutral and acid detergent fibre, cellulose and hemicellulose. It is important to focus on screening and selecting perennial grass with higher nitrogen content and low methane production to mitigate methane production under tropical conditions.

scholarly journals Effects of Thymol Supplementation on Goat Rumen Fermentation and Rumen Microbiota In Vitro

2020 ◽  
Vol 8 (8) ◽  
pp. 1160 ◽  
Author(s):  
Jiangkun Yu ◽  
Liyuan Cai ◽  
Jiacai Zhang ◽  
Ao Yang ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Optimal Dose ◽  
Illumina Miseq ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Rumen Microbiota

This study was performed to explore the predominant responses of rumen microbiota with thymol supplementation as well as effective dose of thymol on rumen fermentation. Thymol at different concentrations, i.e., 0, 100 mg/L, 200 mg/L, and 400 mg/L (four groups × five replications) was applied for 24 h of fermentation in a rumen fluid incubation system. Illumina MiSeq sequencing was applied to investigate the ruminal microbes in addition to the examination of rumen fermentation. Thymol doses reached 200 mg/L and significantly decreased (p < 0.05) total gas production (TGP) and methane production; the production of total volatile fatty acids (VFA), propionate, and ammonia nitrogen, and the digestibility of dry matter and organic matter were apparently decreased (p < 0.05) when the thymol dose reached 400 mg/L. A thymol dose of 200 mg/L significantly affected (p < 0.05) the relative abundance of 14 genera of bacteria, three species of archaea, and two genera of protozoa. Network analysis showed that bacteria, archaea, and protozoa significantly correlated with methane production and VFA production. This study indicates an optimal dose of thymol at 200 mg/L to facilitate rumen fermentation, the critical roles of bacteria in rumen fermentation, and their interactions with the archaea and protozoa.

scholarly journals The nutritive value of stackburned yellow maize for livestock: tests in vitro and in broiler chicks

1996 ◽  
Vol 76 (1) ◽  
pp. 97-108 ◽  
Author(s):  
S. Panigrahi ◽  
L.A. Bestwick ◽  
R.H. Davis ◽  
C. D. Wood
Keyword(s):  
Dietary Fibre ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Reducing Sugar ◽  
Broiler Chicks ◽  
Maillard Reaction Products ◽  
Total Dietary Fibre ◽  
Sheep Rumen

Differences in the nutritive value of four grades of stackburned yellow maize, obtained from a single storage unit in Mozambique, were examined. Samples were analysed for chemical composition, and subjected to the following in vitro assays for estimating digestibility: total dietary fibre and pancreatin for non-ruminants, and gas production using sheep rumen fluid for ruminant livestock. Samples were also fed to broiler chicks at 600 g/kg diet in a growth trial. There were no significant differences in crude protein contents of the maize samples, but there was evidence for the development of Maillard reaction products. Detectable amino acids were lower in discoloured maize, with decreases of 52% in lysine, 35% in arginine, and 15% in glycine concentration in the most severely discoloured sample compared with control. Total starch, reducing sugar, acid-detergent fibre a d amylase-neutral-detergent fibre values increased, while total non-reducing sugar content decreased with increased discolouration. Total dietary fibre and pancreatin assays indicated a lowering in digestibility of maize with increasing discolouration. Weight gain of chicks (P=0·0228), efficiency of feed utilization (P=0·0009) and the metabolizable energy value of diets decreased (P<0·0001) with increasing stackburn discolouration. There were no significant effects on N retention of diets. In vitro fermentation using sheep rumen fluid showed a linear decrease in gas production with increasing maize discolouration, indicating a reduction in rumen degradability with stackburn

Stoichiometry ofin vitrofermentation of pure substrates with particular emphasis on methane production, using the cumulative gas production technique

1996 ◽  
Vol 1996 ◽  
pp. 24-24
Author(s):  
Angela R. Moss ◽  
Karen C. O'Callaghan
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal.In vitrotechniques using rumen fluid as an innoculum can be considered as models ofin vivorumen digestion and have been applied to estimate digestibility of feedstuffs. Thein vitrogas production technique (Menkeet. al., 1979) can be used successfully to estimate this but there has been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.Three pure substrates, glucose (G), pectin (P) and cellulose (C) (lg) were pre-wetted in 94ml of medium D (Theodorouet. al., 1994). inoculated with strained rumen fluid (from wether sheep. 10ml) and incubated without agitation for 72h at 39°C.

scholarly journals Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

2020 ◽  
Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Cassava Root ◽  
Randomized Design ◽  
Completely Randomized Design

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

scholarly journals PSIX-10 In vitro dry matter digestibility, gas production and pH of silages of several maize hybrids

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 415-416
Author(s):  
Pedro Queiroz ◽  
Israel Alves Filho ◽  
Sergio Pereira Junior ◽  
Flavia Scarpino van Cleef ◽  
Jane Ezequiel ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Gas Production ◽  
Measured Data ◽  
Maize Hybrids ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract The objective of this study was to determine the in vitro dry matter digestibility (IVDMD), gas production and pH of silages of 10 maize hybrids in Iturama, Brazil (XB 6012 BT, BM 709 VT PRO2, BM 815 VT PRO2, DKB 363 VT PRO3, AG 8740 VT PRO3, SYN 555 VIP3, SX 8555 VIP3, SX 7005 VIP3, DKB 390 VT PRO3, and RB 9789 VIP3). The maize was ensiled in experimental mini silos in a completely randomized design with 3 repetitions. After 120 d, a subsample from each mini silo was taken and the IVDMD was estimated using Ankom Daisy II and F-57 bags (n = 44). Rumen fluid from two cannulated Nellore steers were mixed and placed into fermenter jars containing Ankom buffers (1:4; v/v). After 48-h incubation (39ºC), 40 mL 6 N HCl and 8 g pepsin were added to each jar and incubated for another 24 h. For gas production and pH evaluations, penicillin-type glass vials (n = 40) were filled with McDougall buffer (20 mL), rumen fluid (10 mL) and substrates (200 mg) and incubated in a shaker (39ºC) for 24 h. The cumulative pressure was measured, vials were opened, and terminal pH was measured. Data were analyzed using the MIXED procedure of SAS. The DKB 363 VT PRO3 presented the greatest IVDMD (84.9%, P = 0.02) and the lowest pH (6.60, P = 0.002). The opposite was observed for DKB 390 VT PRO3, which presented the lowest IVDMD (71.1%) and the greatest pH (6.65). The BM 709 PRO2 produced more gas than the others (246.1 mL/g DM), while SYN 555 VIP3 produced less gas than the others (167.7 mL/g DM, P = 0.0005). In conclusion, the material DKB 363 VT PRO3 presented the most interesting characteristics for use as silage for beef cattle in Iturama, Brazil.

Methane production from a range of feedstuffs as determined in vitro using the cumulative gas production technique and compared with that measured in vivo

1997 ◽  
Vol 1997 ◽  
pp. 194-194
Author(s):  
Angela R. Moss ◽  
D. I. Givens
Keyword(s):  
Methane Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Production Technique

There is a need to be able to measure the methane producing potential of a range of feedstuffs under different rumen conditions in order to predict more accurately the amount of methane produced per animal. In vitro techniques using rumen fluid as an innoculum can be considered as models of in vivo rumen digestion and have been applied to estimate digestibility of feedstuffs. The in vitro gas production technique (Menke et. al, 1979) can be used successfully to estimate this but there have been limited attempts to use it to estimate the methane producing potential of feedstuffs. The objective was to assess the potential of the technique to do this.

scholarly journals Effect of Protection of Soybean Meal Using Mahogany Leaf Extract in Ruminant Diet on Rumen Fermentation Products

2021 ◽  
Vol 26 (3) ◽  
pp. 96
Author(s):  
Merryafinola Ifani ◽  
Efka Aris Rimbawanto ◽  
Fransisca Maria Suhartati
Keyword(s):  
Soybean Meal ◽  
Soluble Protein ◽  
Leaf Extract ◽  
Rumen Fluid ◽  
Protein A ◽  
Rumen Fermentation ◽  
Fermentation Products ◽  
Linear Effect ◽  
Randomized Design

<p class="abstrak3"><span lang="IN">The study </span><span>was </span><span lang="IN">aimed to examine effect of protecting soybean meal using mahogany leaf extract on rumen fermentation products in vitro. The material used was cow rumen fluid, basal ration consisting of concentrate and elephant grass with a ratio of 60:40%, and mahogany leaves. The research was carried out in three stages: mahogany extraction, protein protection using mahogany extract, and in vitro stages. The test was conducted in vitro </span><span>based on</span><span lang="IN"> a completely randomized design (CRD). The treatments consisted of 4 kinds of soybean meal protection with 0% tannin concentration (</span><span>T</span><span lang="IN">0); 1.5% (</span><span>T</span><span lang="IN">1); 3% (</span><span>T</span><span lang="IN">2); and 4.5% (</span><span>T</span><span lang="IN">3). </span><span>D</span><span lang="IN">ata obtained were analyzed by analysis of variance and tested using orthogonal polynomials. </span><span>R</span><span lang="IN">esults showed that  addition of protected soybean meal with mahogany leaf extract had a cubic</span><span>al</span><span lang="IN"> effect on partial VFA, methane gas and post-rumen dissolved protein, a quadratic effect on protozoa, and a linear effect</span><span>on </span><span lang="IN">N-NH<sub>3</sub>, SPM, and RUDP. Giving extra mahogany leaves at a level of 1.5% produced a fermented product that was not different from the control while giving a level of 3% got the highest fermentation product. Giving mahogany leaf extract at a level of 4.5% resulted in the highest SPM, and RUDP but there was a decrease in soluble protein, which indicated the occurrence of overprotection. The addition of 3% mahogany leaf extract effectively increased rumen fermentation products, RUDP, and soluble protein without disturbing the activity of rumen bacteria.</span></p>

scholarly journals Effects of Biochar Produced From Tropical Rice Straw, Corncob and Bamboo Tree at Different Processing Temperatures on in Vitro Rumen Fermentation and Methane Production

2021 ◽  
Author(s):  
Dinh Van Dung ◽  
Le Duc Thao ◽  
Le Duc Ngoan ◽  
Le Dinh Phung ◽  
Hynek Roubík
Keyword(s):  
Rice Straw ◽  
Methane Production ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Feed Additive ◽  
Processing Temperature ◽  
Time Points ◽  
Biomass Resources

Abstract This study aimed to evaluate the effects of biochar produced from tropical biomass resources (rice straw, corncob and bamboo) at different processing temperatures (300, 500 and 700oC) on in vitro rumen fermentation and methane production. Treatments were arranged as a 3x3 factorial with three biomass resources and three biochar processing temperatures. Added biochar occupied 3% of the substrate (DM basic). 250 mg of the air-dried substrate was incubated in 120 ml bottles, which contained 25 ml of mixed rumen fluid and buffer mineral solution. Total gas and methane production, in vitro digestibility of DM, OM, and in vitro rumen fermentation characteristics were determined at three-time points: 4, 24 and 48 hours of the incubation. Results showed that biomass resources and processing temperatures affected gas production at 4, 24 and 48 hours of the incubation (P < 0.02). Interactions between biomass resources and processing temperatures affected gas production at 4 hours (P = 0.06) and 24 hours (P = 0.001). Biomass resources and processing temperatures affected methane production at different time points of the incubation, except the effect of biomass resources at 24 hours (P = 0.406). Increased processing temperature from 300 to 700oC reduced gas and methane production (P < 0.05). Biomass resources affected OM digestibility after 4 and 24 hours of incubation. Processing temperatures and their interaction with biomass resources affected OM digestibility after 48 hours of incubation (P < 0.001). NH3-N concentrations at 24 and 48h were highest for corncob, then rice straw, and lowest for bamboo tree derived biochar (P < 0.05). Increased processing temperatures resulted in higher NH3-N concentrations at 24 and 48 hours of incubation (P < 0.05). To mitigate methane production, biomass resources and processing temperatures should be considered when utilising biochar as feed additive in ruminant diets.

scholarly journals CONCENTRATIONS TEST OF METHANE GAS FROM SWAMP FORAGE SILAGE WITH IN VITRO METHOD

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Sofia Sandi ◽  
Nuni Gofar ◽  
Meisji Liana Sari ◽  
Fitra Yosi ◽  
Budi Untari ◽  
...  
Keyword(s):  
Methane Concentration ◽  
Gas Production ◽  
In Vitro Method ◽  
Gas Concentration ◽  
Methane Gas ◽  
Randomized Design ◽  
Concentration Test ◽  
Completely Randomized Design

Aims of this study to concentration test of methane gas from swamp forage silage with in vitro methods.This study used Completely Randomized Design with 3 treatments and each treatment consisted of 5 replications. The treatments used are as follows: P1 (100% kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% kumpai tembaga grass (Hymenachne acutigluma) + 50% kemon air (Neptunia oleracea lour)), P3 (100% kemon air ( Neptunia oleracea lour)). The parameters observed N-Ammonia, total gas production, methane gas concentrations in vitro and VFA partially. The results of variance showed that treatment significantly different (P <0.05) with methane concentration  in vitro with highest gas concentration treatment of silage made from 50%  kumpai tembaga grass (Hymenachne acutigluma) and  50% kemon air (Neptunia oleracea lour).Keywords: Concentration, Methane, Silage Forage Swamp, In Vitro.

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