The Culture-Fair Common Region Test (CRT)

2020 ◽  
Author(s):  
Christiane Lange-Kuettner ◽  
Ridhi Kochhar
Keyword(s):  
Common Region

scholarly journals Characterization of a Novel Plasmid-Mediated Cephalosporinase (CMY-9) and Its Genetic Environment in an Escherichia coli Clinical Isolate

2002 ◽  
Vol 46 (8) ◽  
pp. 2427-2434 ◽  
Author(s):  
Yohei Doi ◽  
Naohiro Shibata ◽  
Keigo Shibayama ◽  
Kazunari Kamachi ◽  
Hiroshi Kurokawa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Gene Cassette ◽  
Single Amino Acid ◽  
Common Region ◽  
Class 1 Integron ◽  
Class 1 ◽  
The Common ◽  
High Level

ABSTRACT An Escherichia coli strain, HKYM68, which showed resistance to broad-spectrum cephalosporins was isolated from a sputum specimen in Japan. The high-level resistance of the strain to ceftazidime, cefpirome, and moxalactam was carried by a self-transferable plasmid. The β-lactamase gene responsible for the resistance was cloned and sequenced. The deduced amino acid sequence of this gene product, CMY-9, had a single amino acid substitution (E85D), the residue reported to be part of the recognition site for the R1 side chain of β-lactams, compared with the amino acid sequence of CMY-8 and also had 78% identity with the amino acid sequence of CepH, a chromosomal cephalosporinase of Aeromonas hydrophila. A sul1-type class 1 integron containing an aacA1-orfG gene cassette was identified upstream of bla CMY-9 and ended with a truncated 3′ conserved segment. The following 2.1 kb was almost identical to the common region of integrons In6 and In7 and the integron of pSAL-1, except that orf513 encoding a putative transposase was identified instead of orf341 due to addition of a single nucleotide. bla CMY-9 was closely located downstream of the end of the common region. These observations are indicative of the exogenous derivation of bla CMY-9 from some environmental microorganisms such as aeromonads.

scholarly journals Molecular Characterization ofblaNDM-1in a Sequence Type 235 Pseudomonas aeruginosa Isolate from France

2013 ◽  
Vol 57 (7) ◽  
pp. 3408-3411 ◽  
Author(s):  
Frédéric Janvier ◽  
Katy Jeannot ◽  
Sophie Tessé ◽  
Marjorie Robert-Nicoud ◽  
Hervé Delacour ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Insertion Sequence ◽  
Variable Region ◽  
Sequence Type ◽  
Common Region ◽  
Class 1 Integron ◽  
Genetic Studies ◽  
Content Type ◽  
Class 1 ◽  
Previous Hospitalization

ABSTRACTAn NDM-1 carbapenemase-producingPseudomonas aeruginosaisolate was recovered from a patient hospitalized in France after a previous hospitalization in Serbia. Genetic studies revealed that theblaNDM-1gene was surrounded by insertion sequence ISAba125and a truncated bleomycin resistance gene. ThisblaNDM-1region was a part of the variable region of a new complex class 1 integron bearing IS common region 1 (ISCR1). The presence of ISPa7upstream of this integron suggests insertion in a chromosomally located Tn402-like structure.

Ankle injuries

2013 ◽  
Author(s):  
Bill Ribbans ◽  
Cathy Speed
Keyword(s):  
Risk Factors ◽  
Early Recognition ◽  
The Body ◽  
Ankle Injuries ◽  
Modifiable Risk Factors ◽  
Full Potential ◽  
Common Region

The ankle is the most common region of the body to be injured in sport. However, injuries sustained at this site are commonly neglected, resulting in potentially significant long-term functional and structural sequelae.Effective prophylactic strategies to minimize ankle injuries in specific sports should be developed. Addressing modifiable risk factors should be an important aspect of conditioning regimes for most sports, and early recognition and management of injuries is vital for preventing the development of chronic pathology limiting the attainment of an athlete’s full potential....

Loss of heterozygosity on 17p in human breast carcinomas: defining the smallest common region of deletion

1990 ◽  
Vol 53 (1) ◽  
pp. 52-54 ◽  
Author(s):  
P. Devilee ◽  
C.J. Cornelisse ◽  
N Kuipers-Dijkshoorn ◽  
C. Jonker ◽  
P.L. Pearson
Keyword(s):  
Loss Of Heterozygosity ◽  
Breast Carcinomas ◽  
Human Breast ◽  
Common Region ◽  
Human Breast Carcinomas

scholarly journals Evidence of inter-component recombination, intra-component recombination and reassortment in banana bunchy top virus

2012 ◽  
Vol 93 (5) ◽  
pp. 1103-1119 ◽  
Author(s):  
Daisy Stainton ◽  
Simona Kraberger ◽  
Matthew Walters ◽  
Elizabeth J. Wiltshire ◽  
Karyna Rosario ◽  
...  
Keyword(s):  
Protein Gene ◽  
Full Genome ◽  
Genome Sequences ◽  
Tropical Africa ◽  
Common Region ◽  
Banana Bunchy Top Virus ◽  
Recombination Hotspot ◽  
Recombination Hotspots ◽  
The Common ◽  
Ssdna Viruses

Banana bunchy top virus (BBTV; family Nanoviridae, genus Babuvirus) is a multi-component, ssDNA virus, which causes widespread banana crop losses throughout tropical Africa and Australasia. We determined the full genome sequences of 12 BBTV isolates from the Kingdom of Tonga and analysed these together with previously determined BBTV sequences to show that reassortment and both inter- and intra-component recombination have all been relatively frequent occurrences during BBTV evolution. We found that whereas DNA-U3 components display evidence of complex inter- and intra-component recombination, all of the South Pacific DNA-R components have a common intra-component recombinant origin spanning the replication-associated protein gene. Altogether, the DNA-U3 and DNA-M components display a greater degree of inter-component recombination than the DNA-R, -S, -C and -M components. The breakpoint distribution of the inter-component recombination events reveals a primary recombination hotspot around the 5′ side of the common region major and, in accordance with recombination hotspots detectable in related ssDNA viruses, a secondary recombination hotspot near the origin of virion-strand replication.

Identification of a 1-Mb common region at 16q24.1-24.2 deleted in hepatocellular carcinoma

2000 ◽  
Vol 28 (1) ◽  
pp. 38-44 ◽  
Author(s):  
Koichi Bando ◽  
Hisaki Nagai ◽  
Satoshi Matsumoto ◽  
Masaaki Koyama ◽  
Naoki Kawamura ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Common Region

Array CGH Analysis for Target Genes at 6p21 Amplification Region in Diffuse Large B-Cell Lymphoma.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1116-1116
Author(s):  
Yumiko Kasugai ◽  
Hiroyuki Tagawa ◽  
Yoshihiro Kameoka ◽  
Sivasundaram Karnan ◽  
Ritsuro Suzuki ◽  
...  
Keyword(s):  
B Cell ◽  
Target Genes ◽  
Array Cgh ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Cyclin D3 ◽  
Common Region ◽  
Bac Clones ◽  
Large B Cell Lymphoma ◽  
Large B Cell

Abstract Amplification of 6p21 has been detected in various solid tumor and hematological neoplasms. These include hepatoma, osteosarcoma, pancreatic cancer, bladder cancer, and mantle cell lymphoma . However, no candidate target genes in this amplified region have been identified. In diffuse large B-cell lymphoma (DLBCL), no amplification has been reported. Recently, we established genome-wide array comparative genomic hybridization (array CGH) consisting of 2300 BAC clones that can survey a whole genome at the density of an average size of 1.3 Mb. The array CGH detected amplification at 6p21 in 12 of 70 (24%) DLBCL patients (Tagawa et al., Cancer Res. in press) and one DLBCL cell line, SUDHL-9. We next made high density array glass for 6p21 region with 23 BAC clones covering 3 Mb. We found that the minimal common region of amplification was approximately 2Mb in size. In this region, there are 27 known genes including cyclin D3. The cyclin D3 has been reported to be overexpressed in a case of DLBCL with t(6;14)(p21.1;q32.3). We have analyzed expression level of those genes in the minimal common region of amplification, and found that five genes, BYSL, cyclin D3, TBN, KIAA0240, and TBCC were overexpressed. These results indicate that not only cyclin D3 but also other genes are also possible target genes for the 6p21 amplification.

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