scholarly journals From Host to Phage Metabolism: Hot Tales of Phage T4’s Takeover of E. coli

2018 ◽  
Author(s):  
Elizabeth Kutter ◽  
Daniel Bryan ◽  
Georgia Ray ◽  
Erin Brewster ◽  
Bob Blasdel ◽  
...  
Keyword(s):  
Sequence Data ◽  
Bacteriophage T4 ◽  
Infection Process ◽  
E Coli ◽  
Phage T4 ◽  
Progeny Phage ◽  
Wide Range ◽  
Phage Production ◽  
The Subject ◽  
Intense Research

The mechanisms by which bacteriophage T4 converts the metabolism of its E. coli host to one dedicated to progeny phage production was the subject of decades of intense research in many labs from the 1950’s through the 1980’s. At this point, a wide range of phages are starting to be used therapeutically and in many other applications and also the range of available phage sequence data is skyrocketing. It is thus important to re-explore the extensive available data about the intricacies of the T4 infection process as summarized here, expand it to looking much more broadly at other genera of phages, and explore phage infections using newly-available modern techniques and a range of appropriate environmental conditions.

scholarly journals From Host to Phage Metabolism: Hot Tales of Phage T4’s Takeover of E. coli

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 387 ◽  
Author(s):  
Elizabeth Kutter ◽  
Daniel Bryan ◽  
Georgia Ray ◽  
Erin Brewster ◽  
Bob Blasdel ◽  
...  
Keyword(s):  
Sequence Data ◽  
Bacteriophage T4 ◽  
Infection Process ◽  
E Coli ◽  
Phage T4 ◽  
Progeny Phage ◽  
Wide Range ◽  
Phage Production ◽  
The Subject ◽  
Intense Research

The mechanisms by which bacteriophage T4 converts the metabolism of its E. coli host to one dedicated to progeny phage production was the subject of decades of intense research in many labs from the 1950s through the 1980s. Presently, a wide range of phages are starting to be used therapeutically and in many other applications, and also the range of phage sequence data available is skyrocketing. It is thus important to re-explore the extensive available data about the intricacies of the T4 infection process as summarized here, expand it to looking much more broadly at other genera of phages, and explore phage infections using newly-available modern techniques and a range of appropriate environmental conditions.

scholarly journals A Comparative Study of Target Tracking Approaches in Wireless Sensor Networks

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Asmaa Ez-Zaidi ◽  
Said Rakrak
Keyword(s):  
Wireless Sensor Networks ◽  
Sensor Networks ◽  
Target Tracking ◽  
Sensor Nodes ◽  
Wireless Sensor ◽  
Future Directions ◽  
Wide Range ◽  
Comprehensive Survey ◽  
The Subject ◽  
Intense Research

Wireless sensor networks have been the subject of intense research in recent years. Sensor nodes are used in wide range of applications such as security, military, and environmental monitoring. One of the most interesting applications in wireless sensor networks is target tracking, which mainly consists in detecting and monitoring the motion of mobile targets. In this paper, we present a comprehensive survey of target tracking approaches. We then analyze them according to several metrics. We also discuss some of the challenges that influence the performance of tracking schemes. In the end, we conduct detailed analysis and comparison between these algorithms and we conclude with some future directions.

DYNAMICS OF A MODEL FOR VIRULENT PHAGE T4

2008 ◽  
Vol 16 (04) ◽  
pp. 597-611 ◽  
Author(s):  
ZHIPENG QIU
Keyword(s):  
Population Density ◽  
Reproduction Number ◽  
Bacteriophage T4 ◽  
Sufficient Conditions ◽  
Chemostat Model ◽  
Virulent Phage ◽  
E Coli ◽  
Phage T4 ◽  
Theoretical Results ◽  
The Basic Reproduction Number

In this paper, the asymptotical behavior of a chemostat model for E. coli and the virulent phage T4 is analyzed. The basic reproduction number R0 is proved to be a threshold which determines the outcome of the virulent phage T4. If R0 < 1, the virus dies out; if R0 > 1, the virus persists. Sufficient conditions for the Hopf bifurcation are also established. The theoretical results show that increasing the input of nutrient will result in an increase in the equilibrium population density of the virulent bacteriophage T4, but will have no effect on the equilibrium population density of E. coli. The results also show that increasing the input of nutrient or increasing the average lytic time for the infected E. coli can destabilize the interaction between E. coli and T4.

scholarly journals The genetics of the Luria–Latarjet effect in bacteriophage T4: evidence for the involvement of multiple DNA repair pathways

1993 ◽  
Vol 62 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Paul Hyman
Keyword(s):  
Dna Damage ◽  
Excision Repair ◽  
Bacteriophage T4 ◽  
Recombinational Repair ◽  
Second Stage ◽  
Phage T4 ◽  
Progeny Phage ◽  
Study Support ◽  
Repair Pathways ◽  
Two Stages

SummaryThe Luria-Latarjet effect is an increase in resistance of a virus to DNA damage during infection of a host. It has often been assumed to involve recombinational repair, but this has never been demonstrated experimentally. Using nine bacteriophage (phage) T4 mutants, I present evidence indicating that, for phage T4, the Luria-Latarjet effect is due to three repair pathways-excision repair, post-replication-recombinational-repair (PRRR) and multiplicity reactivation (MR) (a second form of recombinational repair). The results also show that the Luria-Latarjet effect develops in two stages. The first stage starts soon after infection. Damage which occurs during the first stage can be repaired by excision repair or PRRR. The second stage appears to start after the first round of DNA replication is complete. DNA damage which occurs during this stage can apparently be repaired by MR as well as the other two repair pathways. The results of this study support the hypothesis that recombinational repair has been selected to ensure that the progeny phage genomes which are packaged have minimum DNA damage. Since other viruses which infect bacterial, animal and plant cells show a Luria-Latarjet effect similar to that in phage T4, the conclusions from this study may have wide applicability.

scholarly journals IDENTIFICATION OF THE BACTERIOPHAGE T4 unf (=alc) GENE PRODUCT, A PROTEIN INVOLVED IN THE SHUTOFF OF HOST TRANSCRIPTION

Genetics ◽  
1984 ◽  
Vol 108 (2) ◽  
pp. 305-317
Author(s):  
Richard E Herman ◽  
Nancy Haas ◽  
D Peter Snustad
Keyword(s):  
Gene Product ◽  
Gel Electrophoresis ◽  
Gene Dosage ◽  
Bacteriophage T4 ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
E Coli ◽  
Phage T4 ◽  
Protein Functions

ABSTRACT The introduction of plasmid pR386 into E. coli cells renders them restrictive to the growth of phage T4 unf (=alc) mutants. This system has been used to isolate Unf+ revertants, which, along with the mutant parental strains, have been used to identify the unf gene product by two-dimensional gel electrophoresis. Synthesis of the unf gene product, a polypeptide of just over 18,000 daltons in size, begins within 1 min after infection and terminates at about 12 min after infection at 30°. Gene dosage experiments suggest that the unf protein functions catalytically.

scholarly journals Escherichia coli mutations that prevent the action of the T4 unf/alc protein map in an RNA polymerase gene.

Genetics ◽  
1988 ◽  
Vol 118 (2) ◽  
pp. 173-180
Author(s):  
L Snyder ◽  
L Jorissen
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Bacteriophage T4 ◽  
Strong Support ◽  
Rpob Gene ◽  
Late Gene Expression ◽  
Gene Encoding ◽  
E Coli ◽  
A Subunit ◽  
Phage Production

Abstract Bacteriophage T4 has the substituted base hydroxymethylcytosine in its DNA and presumably shuts off host transcription by specifically blocking transcription of cytosine-containing DNA. When T4 incorporates cytosine into its own DNA, the shutoff mechanism is directed back at T4, blocking its late gene expression and phage production. Mutations which permit T4 multiplication with cytosine DNA should be in genes required for host shutoff. The only such mutations characterized thus far have been in the phage unf/alc gene. The product of this gene is also required for the unfolding of the host nucleoid after infection, hence its dual name unf/alc. As part of our investigation of the mechanism of action of unf/alc, we have isolated Escherichia coli mutants which propagate cytosine T4 even if the phage are genotypically alc+. These same E. coli mutants are delayed in the T4-induced unfolding of their nucleoid, lending strong support to the conclusion that blocking transcription and unfolding the host nucleoid are but different manifestations of the same activity. We have mapped two of the mutations, called paf mutations for prevent alc function. They both map at about 90 min, probably in the rpoB gene encoding a subunit of RNA polymerase. From the behavior of Paf mutants, we hypothesize that the unf/alc gene product of T4 interacts somehow with the host RNA polymerase to block transcription of cytosine DNA and unfold the host nucleoid.

Typologization of security regulation

2020 ◽  
pp. 20-27
Author(s):  
Denis Tikhomirov
Keyword(s):  
Social Relations ◽  
Scientific Literature ◽  
Legal Regulation ◽  
Procedural Understanding ◽  
Limited Ability ◽  
Methodological Basis ◽  
Wide Range ◽  
The Subject ◽  
Definition Of ◽  
Legal Understanding

The purpose of the article is to typologize terminological definitions of security, to find out the general, to identify the originality of their interpretations depending on the subject of legal regulation. The methodological basis of the study is the methods that made it possible to obtain valid conclusions, in particular, the method of comparison, through which it became possible to correlate different interpretations of the term "security"; method of hermeneutics, which allowed to elaborate texts of normative legal acts of Ukraine, method of typologization, which made it possible to create typologization groups of variants of understanding of the term "security". Scientific novelty. The article analyzes the understanding of the term "security" in various regulatory acts in force in Ukraine. Typological groups were understood to understand the term "security". Conclusions. The analysis of the legal material makes it possible to confirm that the issues of security are within the scope of both legislative regulation and various specialized by-laws. However, today there is no single conception on how to interpret security terminology. This is due both to the wide range of social relations that are the subject of legal regulation and to the relativity of the notion of security itself and the lack of coherence of views on its definition in legal acts and in the scientific literature. The multiplicity of definitions is explained by combinations of material and procedural understanding, static - dynamic, and conditioned by the peculiarities of a particular branch of legal regulation, limited ability to use methods of one or another branch, the inter-branch nature of some variations of security, etc. Separation, common and different in the definition of "security" can be used to further standardize, in fact, the regulatory legal understanding of security to more effectively implement the legal regulation of the security direction.

History of the Study of Apocrypha in Early Medieval England

2020 ◽  
Vol 48 (3-4) ◽  
pp. 13-26
Author(s):  
Brandon W. Hawk
Keyword(s):  
Old Testament ◽  
Medieval England ◽  
Medieval Period ◽  
Early Medieval ◽  
Future Directions ◽  
Early Judaism ◽  
Wide Range ◽  
History Of ◽  
The Subject ◽  
Early English

Literature written in England between about 500 and 1100 CE attests to a wide range of traditions, although it is clear that Christian sources were the most influential. Biblical apocrypha feature prominently across this corpus of literature, as early English authors clearly relied on a range of extra-biblical texts and traditions related to works under the umbrella of what have been called “Old Testament Pseudepigrapha” and “New Testament/Christian Apocrypha." While scholars of pseudepigrapha and apocrypha have long trained their eyes upon literature from the first few centuries of early Judaism and early Christianity, the medieval period has much to offer. This article presents a survey of significant developments and key threads in the history of scholarship on apocrypha in early medieval England. My purpose is not to offer a comprehensive bibliography, but to highlight major studies that have focused on the transmission of specific apocrypha, contributed to knowledge about medieval uses of apocrypha, and shaped the field from the nineteenth century up to the present. Bringing together major publications on the subject presents a striking picture of the state of the field as well as future directions.

Museum Preserves in the Sphere of “Cultural Tourism”: Through the Example of the Cultural Preserves of Tatarstan

2019 ◽  
Vol 9 (4) ◽  
pp. 193-198
Author(s):  
Lyudmila S. Timofeeva ◽  
Albina R. Akhmetova ◽  
Liliya R. Galimzyanova ◽  
Roman R. Nizaev ◽  
Svetlana E. Nikitina
Keyword(s):  
19Th Century ◽  
Art Museum ◽  
Medium Size ◽  
Business Elite ◽  
Wide Range ◽  
The Subject ◽  
Tourist Center ◽  
Regional Authorities ◽  
The 19Th Century ◽  
The City

Abstract The article studies the existence experience of historical cities as centers of tourism development as in the case of Elabuga. The city of Elabuga is among the historical cities of Russia. The major role in the development of the city as a tourist center is played by the Elabuga State Historical-Architectural and Art Museum-Reserve. The object of the research in the article is Elabuga as a medium-size historical city. The subject of the research is the activity of the museum-reserve which contributes to the preservation and development of the historical look of Elabuga and increases its attractiveness to tourists. The tourism attractiveness of Elabuga is obtained primarily through the presence of the perfectly preserved historical center of the city with the blocks of integral buildings of the 19th century. The Elabuga State Historical-Architectural and Art Museum-Reserve, which emerged in 1989, is currently an object of historical and cultural heritage of federal importance. Museum-reserves with their significant territories and rich historical, cultural and natural heritage have unique resources for the implementation of large partnership projects. Such projects are not only aimed at attracting a wide range of tourists, but also stimulate interest in the reserve from the business elite, municipal and regional authorities. The most famous example is the Spasskaya Fair which revived in 2008 in Elabuga. It was held in the city since the second half of the 19th century, and was widely known throughout Russia. The process of the revival and successful development of the fair can be viewed as the creation of a special tourist event contributing to the formation of new and currently important tourism products.

Small Molecule Permeation Across Membrane Channels: Chemical Modification to Quantify Transport Across OmpF

2018 ◽  
Author(s):  
Jiajun Wang ◽  
Jayesh Arun Bafna ◽  
Satya Prathyusha Bhamidimarri ◽  
Mathias Winterhalter
Keyword(s):  
Dwell Time ◽  
Covalent Binding ◽  
Channel Structure ◽  
Ion Current ◽  
E Coli ◽  
Current Fluctuation ◽  
Wide Range ◽  
Outer Membrane Channel ◽  
Biological Channels ◽  
Constriction Region

Biological channels facilitate the exchange of small molecules across membranes, but surprisingly there is a lack of general tools for the identification and quantification of transport (i.e., translocation and binding). Analyzing the ion current fluctuation of a typical channel with its constriction region in the middle does not allow a direct conclusion on successful transport. For this, we created an additional barrier acting as a molecular counter at the exit of the channel. To identify permeation, we mainly read the molecule residence time in the channel lumen as the indicator whether the molecule reached the exit of the channel. As an example, here we use the well-studied porin, OmpF, an outer membrane channel from <i>E. coli</i>. Inspection of the channel structure suggests that aspartic acid at position 181 is located below the constriction region (CR) and we subsequently mutated this residue to cysteine, where else cysteine free and functionalized it by covalent binding with 2-sulfonatoethyl methanethiosulfonate (MTSES) or the larger glutathione (GLT) blockers. Using the dwell time as the signal for transport, we found that both mono-arginine and tri-arginine permeation process is prolonged by 20% and 50% respectively through OmpF<sub>E181C</sub>MTSES, while the larger sized blocker modification OmpF<sub>E181C</sub>GLT drastically decreased the permeation of mono-arginine by 9-fold and even blocked the pathway of the tri-arginine. In case of the hepta-arginine as substrate, both chemical modifications led to an identical ‘blocked’ pattern observed by the dwell time of ion current fluctuation of the OmpF<sub>wt</sub>. As an instance for antibiotic permeation, we analyzed norfloxacin, a fluoroquinolone antimicrobial agent. The modulation of the interaction dwell time suggests possible successful permeation of norfloxacin across OmpF<sub>wt</sub>. This approach may discriminate blockages from translocation events for a wide range of substrates. A potential application could be screening for scaffolds to improve the permeability of antibiotics.

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