Effect of Peg-Coated Silver and Gold Nanoparticles on Spirulina platensis Biomass during Its Growth in Closed System

Silver and gold nanoparticles are a promising tool for medical and industrial application, therefore, their ecotoxicity should be carefully examined. The effect of silver (12 nm) and gold (4.7) nanoparticles coated with polyethylene glycol on Spirulina platensis biomass growth and biochemical composition was investigated. The spirulina cultivation medium was supplemented with nanoparticles in concentration range 0.025-0.5 µM. Given concentrations stimulated spirulina biomass growth, while content of proteins, carbohydrates and auxiliary pigments was affected insignificantly by presence of nanoparticles in cultivation medium. The pronounced effect of gold nanoparticles at concentration 0.5 µM on the lipids content was observed. TEM images demonstrate that nanoparticles penetrate inside the cell, resulting in ultrastructural changes in cells.

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Effects of PEG-Coated Silver and Gold Nanoparticles on Spirulina platensis Biomass during Its Growth in a Closed System

Coatings ◽

10.3390/coatings10080717 ◽

2020 ◽

Vol 10 (8) ◽

pp. 717

Author(s):

Liliana Cepoi ◽

Inga Zinicovscaia ◽

Ludmila Rudi ◽

Tatiana Chiriac ◽

Ion Rotari ◽

...

Keyword(s):

Gold Nanoparticles ◽

Spirulina Platensis ◽

Biochemical Composition ◽

Industrial Applications ◽

Ultrastructural Changes ◽

Cultivation Medium ◽

Silver And Gold Nanoparticles ◽

Low Concentrations ◽

Negative Effect ◽

High Concentrations

Silver and gold nanoparticles are promising tools for medical and industrial applications; therefore, their ecotoxicity should be carefully examined. There are many publications that discuss their effects at high concentrations on various organisms, while the effects of low doses have not been sufficiently investigated. In this paper, the effects of low concentrations of silver (12 nm) and gold (4.7 nm) nanoparticles coated with polyethylene glycol on Spirulina platensis biomass growth, biochemical composition, and antioxidant activity were investigated. The spirulina cultivation medium was supplemented with nanoparticles in the concentration range of 0.025–0.5 µM. The given concentrations stimulated spirulina biomass, but the content of proteins, carbohydrates, and auxiliary pigments was insignificantly affected by the presence of nanoparticles in the cultivation medium. Gold nanoparticles at a concentration of 0.5 µM produced a pronounced effect on the lipid content. Transmission electron microscope images demonstrated that the nanoparticles penetrate inside the cells and cause ultrastructural changes. The nanoparticles were characterized using several well-known techniques. The results confirmed a negative effect of low concentrations of metal nanoparticles on spirulina. This effect could be indiscernible when studying the biomass viability, but determination of the ultrastructure of the cell and the biochemical composition of the biomass could reveal it.

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Biomedical Applications of Silver and Gold Nanoparticles: Effective and Safe Non-Viral Delivery Vehicles

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2017.03.00059 ◽

2017 ◽

Vol 3 (2) ◽

Cited By ~ 4

Author(s):

Kirti Rani

Keyword(s):

Gold Nanoparticles ◽

Biomedical Applications ◽

Silver And Gold Nanoparticles ◽

Delivery Vehicles

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Seed-mediated synthesis and PEG coating of gold nanoparticles for controlling morphology and sizes

MRS Advances ◽

10.1557/adv.2020.416 ◽

2020 ◽

Vol 5 (63) ◽

pp. 3353-3360

Author(s):

Susana Helena Arellano Ramírez ◽

Perla García Casillas ◽

Christian Chapa González

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Room Temperature ◽

Colloidal Stability ◽

Infrared Spectrometry ◽

Ammonium Bromide ◽

Chloroauric Acid ◽

Vis Spectroscopy ◽

Seed Mediated ◽

Peg Coating

AbstractA significant area of research is biomedical applications of nanoparticles which involves efforts to control the physicochemical properties through simple and scalable processes. Gold nanoparticles have received considerable attention due to their unique properties that they exhibit based on their morphology. Gold nanospheres (AuNSs) and nanorods (AuNRs) were prepared with a seed-mediated method followed of polyethylene glycol (PEG)-coating. The seeds were prepared with 0.1 M cetyltrimethyl-ammonium bromide (CTAB), 0.005 M chloroauric acid (HAuCl4), and 0.01 M sodium borohydride (NaBH4) solution. Gold nanoparticles with spherical morphology was achieved by growth by aggregation at room temperature, while to achieve the rod morphology 0.1 M silver nitrate (AgNO3) and 0.1 M ascorbic acid solution were added. The gold nanoparticles obtained by the seed-mediated synthesis have spherical or rod shapes, depending on the experimental conditions, and a uniform particle size. Surface functionalization was developed using polyethylene glycol. Morphology, and size distribution of AuNPs were evaluated by Field Emission Scanning Electron Microscopy. The average size of AuNSs, and AuNRs was 7.85nm and 7.96 x 31.47nm respectively. Fourier transform infrared spectrometry was performed to corroborate the presence of PEG in the AuNPs surface. Additionally, suspensions of AuNSs and AuNRs were evaluated by UV-Vis spectroscopy. Gold nanoparticles were stored for several days at room temperature and it was observed that the colloidal stability increased once gold nanoparticles were coated with PEG due to the shield formed in the surface of the NPs and the increase in size which were 9.65±1.90 nm of diameter for AuNSs and for AuNRs were 29.03±5.88 and 8.39±1.02 nm for length and transverse axis, respectively.

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Physiologically-based pharmacokinetic modeling of different size polyethylene glycol-coated gold nanoparticles in mice

10.1615/ihtc16.nmt.023362 ◽

2018 ◽

Author(s):

Linjing Deng ◽

Jinping Zhao ◽

Yufeng Miao ◽

Xu Hu ◽

Qihong Deng

Keyword(s):

Gold Nanoparticles ◽

Polyethylene Glycol ◽

Pharmacokinetic Modeling ◽

Physiologically Based Pharmacokinetic Modeling ◽

Physiologically Based Pharmacokinetic ◽

Physiologically Based

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Antibacterial and Anti-Biofilm Biosynthesised Silver and Gold Nanoparticles for Medical Applications: Mechanism of Action, Toxicity and Current Status

Current Drug Delivery ◽

10.2174/1567201817666191227094334 ◽

2020 ◽

Vol 17 (2) ◽

pp. 88-100 ◽

Cited By ~ 8

Author(s):

Sundos Suleman Ismail Abdalla ◽

Haliza Katas ◽

Fazren Azmi ◽

Mohd Fauzi Mh Busra

Keyword(s):

Gold Nanoparticles ◽

Synthesis Method ◽

Chemical Characterization ◽

Current Status ◽

Silver And Gold Nanoparticles ◽

Physical And Chemical Characterization ◽

Biosynthesized Agnps ◽

Physical And Chemical ◽

Insight Into ◽

Bio Synthesis

Fast progress in nanoscience and nanotechnology has contributed to the way in which people diagnose, combat, and overcome various diseases differently from the conventional methods. Metal nanoparticles, mainly silver and gold nanoparticles (AgNPs and AuNPs, respectively), are currently developed for many applications in the medical and pharmaceutical area including as antibacterial, antibiofilm as well as anti-leshmanial agents, drug delivery systems, diagnostics tools, as well as being included in personal care products and cosmetics. In this review, the preparation of AgNPs and AuNPs using different methods is discussed, particularly the green or bio- synthesis method as well as common methods used for their physical and chemical characterization. In addition, the mechanisms of the antimicrobial and anti-biofilm activity of AgNPs and AuNPs are discussed, along with the toxicity of both nanoparticles. The review will provide insight into the potential of biosynthesized AgNPs and AuNPs as antimicrobial nanomaterial agents for future use.

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Stabilization of Silver and Gold Nanoparticles: Preservation and Improvement of Plasmonic Functionalities

Chemical Reviews ◽

10.1021/acs.chemrev.8b00341 ◽

2018 ◽

Vol 119 (1) ◽

pp. 664-699 ◽

Cited By ~ 107

Author(s):

Hyunho Kang ◽

Joseph T. Buchman ◽

Rebeca S. Rodriguez ◽

Hattie L. Ring ◽

Jiayi He ◽

...

Keyword(s):

Gold Nanoparticles ◽

Silver And Gold Nanoparticles

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Interaction of Differently Sized, Shaped, and Functionalized Silver and Gold Nanoparticles with Glycosylated versus Nonglycosylated Transferrin

ACS Applied Materials & Interfaces ◽

10.1021/acsami.1c04063 ◽

2021 ◽

Author(s):

Rinea Barbir ◽

Rafael Ramírez Jiménez ◽

Rafael Martín-Rapún ◽

Vida Strasser ◽

Darija Domazet Jurašin ◽

...

Keyword(s):

Gold Nanoparticles ◽

Silver And Gold Nanoparticles

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Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications

Molecules ◽

10.3390/molecules26040844 ◽

2021 ◽

Vol 26 (4) ◽

pp. 844 ◽

Cited By ~ 2

Author(s):

Andrea Rónavári ◽

Nóra Igaz ◽

Dóra I. Adamecz ◽

Bettina Szerencsés ◽

Csaba Molnar ◽

...

Keyword(s):

Gold Nanoparticles ◽

Biomedical Applications ◽

Biological Activities ◽

Large Body ◽

Chemical Properties ◽

Biological Synthesis ◽

Silver And Gold Nanoparticles ◽

Comprehensive Collection ◽

Synthetic Approaches ◽

Physical And Chemical

The nanomaterial industry generates gigantic quantities of metal-based nanomaterials for various technological and biomedical applications; however, concomitantly, it places a massive burden on the environment by utilizing toxic chemicals for the production process and leaving hazardous waste materials behind. Moreover, the employed, often unpleasant chemicals can affect the biocompatibility of the generated particles and severely restrict their application possibilities. On these grounds, green synthetic approaches have emerged, offering eco-friendly, sustainable, nature-derived alternative production methods, thus attenuating the ecological footprint of the nanomaterial industry. In the last decade, a plethora of biological materials has been tested to probe their suitability for nanomaterial synthesis. Although most of these approaches were successful, a large body of evidence indicates that the green material or entity used for the production would substantially define the physical and chemical properties and as a consequence, the biological activities of the obtained nanomaterials. The present review provides a comprehensive collection of the most recent green methodologies, surveys the major nanoparticle characterization techniques and screens the effects triggered by the obtained nanomaterials in various living systems to give an impression on the biomedical potential of green synthesized silver and gold nanoparticles.

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Size and Shape-Dependent Antimicrobial Activities of Silver and Gold Nanoparticles: A Model Study as Potential Fungicides

Molecules ◽

10.3390/molecules25112682 ◽

2020 ◽

Vol 25 (11) ◽

pp. 2682 ◽

Cited By ~ 2

Author(s):

Francis J. Osonga ◽

Ali Akgul ◽

Idris Yazgan ◽

Ayfer Akgul ◽

Gaddi B. Eshun ◽

...

Keyword(s):

Gold Nanoparticles ◽

Pathogenic Fungi ◽

Antimicrobial Activities ◽

Plant Diseases ◽

Production Plant ◽

Silver And Gold Nanoparticles ◽

Size And Shape ◽

Model Study ◽

Pathogenic Microbes ◽

Bacteria And Fungi

Plant-based pathogenic microbes hinder the yield and quality of food production. Plant diseases have caused an increase in food costs due to crop destruction. There is a need to develop novel methods that can target and mitigate pathogenic microbes. This study focuses on investigating the effects of luteolin tetraphosphate derived silver nanoparticles (LTP-AgNPs) and gold nanoparticles (LTP-AuNPs) as a therapeutic agent on the growth and expression of plant-based bacteria and fungi. In this study, the silver and gold nanoparticles were synthesized at room temperature using luteolin tetraphosphate (LTP) as the reducing and capping agents. The synthesis of LTP-AgNPs and LTP-AuNP was characterized by Transmission Electron Microscopy (TEM) and size distribution. The TEM images of both LTP-AgNPs and LTP-AuNPs showed different sizes and shapes (spherical, quasi-spherical, and cuboidal). The antimicrobial test was conducted using fungi: Aspergillus nidulans, Trichaptum biforme, Penicillium italicum, Fusarium oxysporum, and Colletotrichum gloeosporioides, while the class of bacteria employed include Pseudomonas aeruginosa, Aeromonas hydrophila, Escherichia coli, and Citrobacter freundii as Gram (−) bacteria, and Listeria monocytogenes and Staphylococcus epidermidis as Gram (+) bacterium. The antifungal study demonstrated the selective size and shape-dependent capabilities in which smaller sized spherical (9 nm) and quasi-spherical (21 nm) AgNPs exhibited 100% inhibition of the tested fungi and bacteria. The LTP-AgNPs exhibited a higher antimicrobial activity than LTP-AuNPs. We have demonstrated that smaller sized AgNPs showed excellent inhibition of A. nidulans growth compared to the larger size nanoparticles. These results suggest that LTP-AuNP and LTP-AgNPs could be used to address the detection and remediation of pathogenic fungi, respectively.

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