scholarly journals Cryopreservation for Tree Species with Recalcitrant Seeds: the Avocado Case

2020 ◽  
Author(s):  
Chris O'Brien ◽  
Jayeni Hiti-Bandaralage ◽  
Raquel Folgado ◽  
Alice Hayward ◽  
Sean Lahmeyer ◽  
...  
Keyword(s):  
Structural Integrity ◽  
Mangifera Indica ◽  
Shoot Tips ◽  
Persea Americana ◽  
Freezing Stress ◽  
Conservation Strategies ◽  
Recalcitrant Seeds ◽  
Embryonic Axes ◽  
Durio Zibethinus

Recent developments in the cryopreservation space has increased the trend in germplasm collections established through cryopreserved in vitro material. Cryopreservation of recalcitrant seeds through embryos and embryonic axes, is not uncommon. Tropical and sub-tropical plants are not acclimated to the cold season, therefore have no in-built natural resilience to the cold. Also, larger seeds from trees, such as avocado (Persea americana Mill.), mango (Mangifera indica) and durian (Durio zibethinus L.) are sensitive to desiccation, chilling and freezing stress, making them unsuitable for seed banking or cryopreservation. Alternatively, as seeds do not carry the same genetic make-up as the mother plant, especially in the context of woody rainforest species of which the cross-pollination is dominant; seed conservation does not serve the purpose of germplasm preservation. Other plant material and methods are needed for these plants to be successfully stored in liquid nitrogen (LN). One such method commonly used is shoot-tip cryopreservation which ensures the clonal fidelity of germplasm. There are many problems when using shoot tips of tropical recalcitrant-seeded species. These include: 1) the toxic effects of cryoprotective agents towards structural integrity; 2) optimum developmental stage for success and 3) oxidative stress associated with excision injury leading to necrosis triggering cell death and hindering regeneration for the shoot tips in culture. A pre-requisite for any cryopreservation system is the availability of an established tissue culture regeneration platform. This review will outline conservation strategies for avocado with special emphasis on attempts and improvements made in the cryopreservation space for storing this horticulturally important crop ‘avocado’ at ultra-low temperatures.

scholarly journals INVENTARISASI HASIL HUTAN BUKAN KAYU PADA TANAMAN MPTS DI HUTAN DESA SUKARAJA KPH RAJABASA

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Ani Fitriyani ◽  
Melya Riniarti ◽  
Duryat Duryat
Keyword(s):  
Random Sampling ◽  
Mangifera Indica ◽  
Forest Products ◽  
Simple Random Sampling ◽  
Agroforestry System ◽  
Persea Americana ◽  
Durio Zibethinus ◽  
The People ◽  
Non Timber Forest Products ◽  
Gnetum Gnemon

ABSTRAK Hutan Desa Sukaraja merupakan hutan lindung yang dimanfaatkan oleh masyarakat melalui pengelolaan dengan sistem agroforestri dan pemungutan hasil hutan bukan kayu untuk meningkatkan kesejahteraan masyarakat. Penelitian ini bertujuan untuk memperoleh data mengenai jenis dan jumlah HHBK dari tanaman MPTs serta menduga potensi HHBK dari tanaman MPTs pada masa yang akan datang berdasarkan ketersediaannya di masa kini. Data dikumpulkan melalui analisis vegetasi pada 29 plot contoh yang diambil berdasarkan metode SRS (Simple Random Sampling). Untuk memprediksi penambahan jumlah MPTs 1 sampai 4 tahun yang akan datang dilakukan pengamatan pohon pada fase tiang dan pancang. Hasil penelitian menunjukkan terdapat 11 jenis tanaman MPTs yang dimanfaatkan hasil hutan bukan kayunya oleh masyarakat Desa Sukaraja yaitu durian, cengkeh, pala, petai, alpukat, kemiri, mangga, nangka, jengkol, melinjo dan duku. Hasil penelitian menunjukkan bahwa hingga beberapa tahun yang akan datang pohon cengkeh dan durian masih menjadi MPTs yang paling banyak dimanfaatkan dan ditanam oleh masyarakat. Sedangkan mangga dan kemiri merupakan HHBK yang produksinya akan stagnan atau bahkan mengalami penurunan dalam kurun waktu 1-4 tahun yang akan datang. Kata Kunci : HHBK, MPTs, Hutan Desa, KHP Rajabasa  ABSTRACT Sukaraja Village Forest is a protected forest that is utilized by the community through agroforestry system and collection of non-timber forest products to improved community welfare. This study aimed to obtain data on the types and numbers of NTFPs from MPTs and to estimate the potential of NTFPs from MPTs in the future based on their availability in the present. Data was collected through vegetation analysis with 29 sample plots taken based on the SRS (Simple Random Sampling) method. To predict the increase of amount of MPTs in 1-4 years, observed of trees in the pole and sapling phases. The results showed that there were 11 types of MPTs that were utilized by non-timber forest products by the people of Sukaraja Village, that were Durio Zibethinus, Eugenis aromaticum, Phitecellobium lobatum, Parkia spesiosa, myristica fragnans, Artocarpus heterophyllus, Lansium domesticum, Persea americana, Alueuritas moluccanus, Gnetum gnemon and Mangifera indica. The results showed that for the next few years Eugenia aromaticum and Durio zibethinus trees were still the most widely used and planted by the community. While production of Mangifera indica and Alleurites moluccanus were the NTFPs that will be stagnate or even decline in the next 1-4 years. Keywords : NTFPs, MPTs, Village Forest, KPH Rajabasa

scholarly journals EFFECT OF NaCl AND CaCl2 ON EMBRYONIC AXES DEVELOPMENT OF CRIOLLO AND ‘HASS’ OF Persea Americana Mill GROWING in vitro

2010 ◽  
Vol XVI (3) ◽  
pp. 161-167
Author(s):  
Leobarda Guadalupe Ramírez-Guerrero ◽  
◽  
Héctor González-Rosas ◽  
Guillermo Calderón-Zavala ◽  
Juan Velázquez Mendoza ◽  
...  
Keyword(s):  
Persea Americana ◽  
Embryonic Axes

scholarly journals PLANT REGENERATION OF AVOCADO (PERSEA AMERICANA MILL) IN VITRO

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696d-696
Author(s):  
Yaseen Mohamed-Yaseen ◽  
Raymond J. Schnell ◽  
Robert J. Knight ◽  
T.L. Davenport
Keyword(s):  
Tissue Culture ◽  
Multiple Shoots ◽  
Persea Americana ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Embryonic Axes ◽  
Phenolic Oxidation

A procedure was developed to regenerate plants via tissue culture from embryonic axes of mature avocado seeds. Explants were cultured in Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and naphthalene-acetic acid (NAA) or thidiazuron (TDZ) and NAA. Culture were kept in the dark for 7-10 days to reduce browning resulting from phenolic oxidation. Multiple shoots (5-8) were formed after transfer to light. Further multiplication were achieved using different combination of BA and NAA or TDZ and NAA. Shoots were cultured in MS supplemented with 2mg/l indolebutyric acid (IBA) for 2 weeks then transferred to MS supplemented with lg/l activated charcoal for root induction. Complete plants were obtained in vitro.

scholarly journals Identification of Tropical Plants Leaves Image Base on Principal Component Analysis

2020 ◽  
Vol 4 (1) ◽  
pp. 64-75
Author(s):  
Indra Laksmana ◽  
Hendra Hendra ◽  
Sri Aulia Novita ◽  
Fithra Herdian ◽  
Mohamad Riza Nurtam ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Plant Species ◽  
Mangifera Indica ◽  
Leaf Shape ◽  
Principal Component ◽  
Component Analysis ◽  
Persea Americana ◽  
Commission Error ◽  
Tropical Plants ◽  
Durio Zibethinus

Difference and variation of leaves shape is usually used as primary identifier of the plant species. But some plants may have a similar leaf shape and thus require another more accurate identifier. This study applied principal component analysis (PCA) methods for identifying tropical plant species from the shape of the leaves. This method simplified the observed variables by reducing the dimensions of the information that is stored as much as 75%, so it did not eliminate important information and can save the data processing time. There were 100 images of leaves taken from several sides of the leaf in JPEG format with which the shape of leaves were look similar, like citrus (Citrus aurantifolia), durian (Durio zibethinus), guava (Psidium guajava), mango (Mangifera indica), jackfruit (Artocarpus heterophyllus), avocado (Persea americana), rambutan (Nephelium lappaceum), sapodilla (Manilkara zapota), red betel (Piper crocatum) and soursop (Annona muricata). Identification of those 10 kind plant leaves produced 97% accuracy rate. Measurement systems were designed using the K-fold Cross Validation with k = 10, the results of experiments shown omission error occurs on the leaves of guava, jackfruit and red betel while twice commission error were found on the leaves sapodilla and once on citrus leaves.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Hydrogels: A Versatile Drug Delivery Carrier Systems

1970 ◽  
Vol 5 (3) ◽  
pp. 1745-1756
Author(s):  
L H Baldaniya ◽  
Sarkhejiya N A
Keyword(s):  
Drug Delivery ◽  
Protein Delivery ◽  
Structural Integrity ◽  
Healing Process ◽  
Polymer Chains ◽  
Wound Healing Process ◽  
Aqueous Environment ◽  
Preparation Methods ◽  
Control Drug

Hydrogels are the material of choice for many applications in regenerative medicine due to their unique properties including biocompatibility, flexible methods of synthesis, range of constituents, and desirable physical characteristics. Hydrogel (also called Aquagel) is a network of polymer chains that are hydrophilic, sometimes found as a colloidal gel in which water is the dispersion medium. Hydrogels are highly absorbent (contain ~99.9% water), natural or synthetic polymers. Hydrogel also possess a degree of flexibility very similar to natural tissue, due to its significant water content. It can serve as scaffolds that provide structural integrity to tissue constructs, control drug and protein delivery to tissues and cultures. Also serve as adhesives or barriers between tissue and material surfaces. The positive effect of hydrogels on wounds and enhanced wound healing process has been proven. Hydrogels provide a warm, moist environment for wound that makes it heal faster in addition to its useful mucoadhesive properties. Moreover, hydrogels can be used as carriers for liposomes containing variety of drugs, such as antimicrobial drugs. Hydrogels are water swollen polymer matrices, with a tendency to imbibe water when placed in aqueous environment. This ability to swell, under biological conditions, makes it an ideal material for use in drug delivery and immobilization of proteins, peptides, and other biological compounds. Hydrogels have been extensively investigated for use as constructs to engineer tissues in vitro. This review describes the properties, classification, preparation methods, applications, various monomer used in formulation and development of hydrogel products.

Assessing the Antimalarial Potentials of Phytochemicals: Virtual Screening, Molecular Dynamics and In-Vitro Investigations

2019 ◽  
Vol 16 (3) ◽  
pp. 291-300
Author(s):  
Saumya K. Patel ◽  
Mohd Athar ◽  
Prakash C. Jha ◽  
Vijay M. Khedkar ◽  
Yogesh Jasrai ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Structural Integrity ◽  
Md Simulations ◽  
Tylophora Indica ◽  
Multidrug Resistance Protein 1 ◽  
Receptor Complexes ◽  
Resistance Protein ◽  
Dynamics Simulations ◽  
Stable Trajectory

Background: Combined in-silico and in-vitro approaches were adopted to investigate the antiplasmodial activity of Catharanthus roseus and Tylophora indica plant extracts as well as their isolated components (vinblastine, vincristine and tylophorine). </P><P> Methods: We employed molecular docking to prioritize phytochemicals from a library of 26 compounds against Plasmodium falciparum multidrug-resistance protein 1 (PfMDR1). Furthermore, Molecular Dynamics (MD) simulations were performed for a duration of 10 ns to estimate the dynamical structural integrity of ligand-receptor complexes. </P><P> Results: The retrieved bioactive compounds viz. tylophorine, vinblastin and vincristine were found to exhibit significant interacting behaviour; as validated by in-vitro studies on chloroquine sensitive (3D7) as well as chloroquine resistant (RKL9) strain. Moreover, they also displayed stable trajectory (RMSD, RMSF) and molecular properties with consistent interaction profile in molecular dynamics simulations. </P><P> Conclusion: We anticipate that the retrieved phytochemicals can serve as the potential hits and presented findings would be helpful for the designing of malarial therapeutics.

Encapsulation-dehydration based cryopreservation of Rauwolfia shoot tips and their regeneration potential in vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 82-87
Author(s):  
P. KISKU ◽  
S. SAHU ◽  
U. SALMA ◽  
S. SINHA RAY ◽  
P. SAHA ◽  
...  
Keyword(s):  
Shoot Tips ◽  
Regeneration Potential

scholarly journals Gold nanoparticles affect the cryopreservation efficiency of in vitro-derived shoot tips of bleeding heart

2021 ◽  
Author(s):  
Dariusz Kulus ◽  
Alicja Tymoszuk
Keyword(s):  
Gold Nanoparticles ◽  
Alginate Bead ◽  
Stability Index ◽  
Shoot Tips ◽  
Start Codon ◽  
Lamprocapnos Spectabilis ◽  
Colloid Dispersion ◽  
Recovery Medium ◽  
Alginate Matrix

AbstractThe popularity of nanoparticles (NPs) is continuously increasing. To date, however, there has been little research on the application of NPs in plant cryopreservation, i.e. storage of tissues in liquid nitrogen (LN). The aim of this study is to analyze the effect and evaluate the usefulness of gold nanoparticles (AuNPs) in regard to cryobiology studies. In vitro-derived shoot tips of Lamprocapnos spectabilis ‘Valentine’ were cryopreserved with the encapsulation-vitrification protocol. Gold nanoparticles (at 10–30 ppm concentration; 13 nm in size) were added either into the preculture medium; to the protective bead matrix during encapsulation; or to the recovery medium after rewarming of samples. The control plants were produced from cryopreserved explants non-treated with nanoparticles or treated with colloid dispersion medium without NPs. A non-LN-treated standard was also considered. The influence of AuNPs on the cryopreservation efficiency was determined by evaluating the recovery rate of explants and their morphogenic response; the membrane stability index (MSI); the concentration of pigments in shoots; and the antioxidant enzymes activity. The genetic stability of the plant material was evaluated using Start Codon Targeted Polymorphism (SCoT) markers. It was found that 10 ppm of AuNPs added into the alginate bead matrix improved the recovery level of LN-derived shoot tips (70.0%) compared to the non-NPs-treated cryopreserved control (50.5%). On the other hand, the presence of nanoparticles in the recovery medium had a deleterious effect on the survival of explants. AuNPs usually had no impact on the MSI (73.9–85.9%), except for those added into the recovery medium at the concentration of 30 ppm (decline to 55.8%). All LN-derived shoots were shorter and contained less chlorophyll and carotenoids than the untreated standard. Moreover, the application of AuNPs affected the enzymatic activity in L. spectabilis. Minor genetic variation was found in 8.6% of plants if AuNPs were added either into the preculture medium (at 10 and 20 ppm) or to the alginate matrix (at 30 ppm). In conclusion, AuNPs added at a lower concentration (10 ppm) into the protective bead matrix can significantly improve the cryopreservation efficiency in L. spectabilis with no alternation in the DNA sequence.

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