scholarly journals Nutritional Value of Five Rangeland Plants Using Gas Production Technique

Author(s):  
Ebrahim Talebi ◽  
Mostafa Yousef-Elahi ◽  
Mohammad Reza Dehghani ◽  
Asghar Salmani

This investigation was conducted to determine the chemical composition and nutritional value of five plant species commonly used as ruminant feeds namely: Artemisia herba-alba, Acer monspessulanum, Amygdalus lycoides, Amygdalus scoparia, and Atriplex leucoclada. After the collecting samples, the chemical compositions of plants included dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), ash (CA), cell wall (NDF), and non-lignin cell wall (ADF) were determined according to standard methods. For degradation testing, the nylon bag technique was applied using three native Sistani fistula calves. Organic matter digestibility (OMD) and metabolisable energy (ME) were determined through the gas production technique. The CP value ranged from 5.30 (Amygdalus scoparia) to 11.72% (Atriplex leucoclada) while NDF value ranged from 52.62 (Amygdalus lycoides) to 69.05% (Amygdalus scoparia). The range of OMD, DOMD, and ME was from 36.67 to 53.27%, 34.67 to 49.11%, and 5.57 to 8.08 (MJ/kg), respectively. The results showed a positive correlation between cell wall composition and dry matter digestibility in plant species. The nutritional value of Amygdalus lycoides, Atriplex leucoclada, Acer monspessulanum, and Artemisia herba-alba was acceptable composition and digestibility.

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.


2017 ◽  
Vol 2 (2) ◽  
pp. 1-6
Author(s):  
H. Husnaeni ◽  
M. Arifin Amril ◽  
Sjamsuddin Rasjid

This research aims to improve the digestibility and nutritional value of rice straw as a ruminant animal feed and determine the effect of long soaking in sea water of rice straw on digestibility in vitro dry matter and organic matter. The used materials in this study are a bales machine, weighing scales, rapiah rope, poly bags, rice straw varieties Ciliwung obtained from rice fields in Bantimurung, sea water with 2.71% saline, as well as tools and chemicals for analysis of in vitro dry matter and organic matter digestibility with pepsin, calculus method. This study is done based on Randomized Complete  Design (RCD) with 5 treatments and 4 replications. Variance based on the long soaking treatment gives the nature of the response curve linear on the digestibility of dry matter and also to organic matter digestibility. The magnitude of the correlation length relationship soaking rice straw and with sea water for in vitro dry matter and organic matter digestibility that is 96.5% and 96.8% respectively. Based on the results of research and discussion can be concluded that rice straw is soaked with sea water 3-12 days producing dry matter digestibility and organic matter are higher than the rice straw that is not soaked with sea water. Soaking of the sea water for 3-12 days increase the digestibility of dry matter and organic matter.


2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.


1969 ◽  
Vol 9 (40) ◽  
pp. 502 ◽  
Author(s):  
MJ Playne

Two experiments were done on the digestibility of Townsville lucerne seed with pod by sheep in metabolism cages. In one experiment, all sheep were fed the same amount (454 g dry matter seed with pod each day) and mean dry matter digestibility was 58.6 � 3.4 per cent. In the other, three different levels of seed with pod were fed, and mean organic matter digestibility ranged from 54.0 � 1.2 to 68.3 4 1.8 per cent, but digestibility was not significantly inversely related to mean organic matter intake which ranged from 438 � 69 to 335 & 10 g/day (P>0.05). The seed with pod contained, as per cent of dry matter, 5.54 per cent nitrogen, 0.36 per cent phosphorus, 0.30 per cent sulphur, and 0.88 per cent calcium. The sheep were in positive nitrogen balance, but in negative phosphorus balance. Between 8 and 14 per cent of actual seed consumed was defaecated unbroken. Up to 25 per cent of the faecal organic matter was unbroken seed, which was of high germination capacity (76 per cent). Amount of defaecated seed (P<0.001) and the defaecated seed as a percentage of faecal organic matter (P<0.02) were linearly related to amount of actual seed consumed. Factors affecting the amount of undamaged seed in the faeces and a possible technique for assessing seed consumption by grazing animals are discussed.


1998 ◽  
Vol 22 ◽  
pp. 227-230
Author(s):  
I. R. Lane ◽  
W. Boying

The polyphenol concentration in various tree fodders has been assayed by the gas production technique using rumen fluid inoculum either by fermentation of whole samples +/- a polyphenol adsorbent (Khazaal et al., 1994) or simply by the reduction of gas production caused by acetone extracts of the fodders (Wood and Plumb, 1995). Successful replacement of rumen fluid as a source of anaerobic bacteria by faeces from ruminant animals has been reported both for two-stage Tilley and Terry dry-matter digestibility (DMD) (El Shaer et al., 1987) and for the gas production technique (Davies, 1991). The use of faeces based inoculum +/- polyphenol adsorbents was therefore tested and optimized for fallen paulownia and poplar leaves using the gas production technique (the trees were commercial hybrids of Paulownia and Populus species respectively). A series of trials was carried out to: develop the use of preliminary incubation of the faeces inoculum; optimize the adsorbent type, molecular weight (MW) and quantity; optimize the faeces concentration in the inoculum and the use of glucose as a pre-incubation stimulant; and evaluate the use of differences in Tl /2 for gas production and end DMD +/- adsorbent to assess the effect of anti-nutritive compounds (ANCs) on the relative rate and extent of fermentation.All trials were carried out using 100 ml plastic vaccine flexibottles as containers maintained at 39°C in water baths. Gas was manually extracted with disposable syringes, generally at 3, 6, 9,12,15,18, 21, 24, 30, 36, 42, 48, 60, 72 h and bottles were well shaken at these times.


1971 ◽  
Vol 11 (48) ◽  
pp. 18 ◽  
Author(s):  
DJ Minson

The digestibility and voluntary intake of P. coloratum CV. Kabulabula, P. coloratum var. Makarikariense CV. Burnett and C.P.I. 13372, P. maximum var. trichoglume CV. Petrie Green Panic, P. maximum cv. Coloniao guinea grass and Hamil were measured with sheep in metabolism pens. Each grass was cut eight to ten times at different growth stages and times of the year. Varieties differed (P<0.01) in their dry matter and organic matter digestibility but the maximum mean difference between varieties was only 2.8 and 3.4 per cent respectively. There were large differences in voluntary intake between grasses. P. maximum cv. Hamil had a voluntary intake 50 and 27 per cent greater than P. coloratum cv. Kabulabula when both had p dry matter digestibility of 50 and 60 per cent respectively. Voluntary intake of digestible organic matter of P. maximum CV. Hamil was 26 per cent higher than that of P. coloratum CV. Kabulabula. The higher intake of P. maximm occurred despite its having higher silicon percentages than P. coloratum. It was concluded that because of the biased relation between intake and digestibility selection of grasses on the basis of digestibility determinations alone could be a misleading guide to their nutritional value.


2021 ◽  
Vol 19 (3) ◽  
pp. 274
Author(s):  
Harwanto Harwanto ◽  
Eko Hendarto ◽  
Bahrun Bahrun ◽  
Joni Johanda Putra ◽  
Nur Hidayat

<p class="MDPI17abstract"><strong>Objective: </strong>This study aims to determine the nutritional value and nutrient digestibility by in vitro of fodder sorghum (<em>Sorghum bicolor</em> (L.) Moench) from the effect of the addition of fermented urine fertilizer to the hydroponic media.</p><p class="MDPI17abstract"><strong>Methods: </strong>The research used sorghum Numbu varieties grown hydroponically in the fodder phase, which was carried out in June - September 2020. The research treatments consist of water medium (T1), fermented urine 12.5 mL/L (T2) and 25 mL/L media (T3) with 4 replications. The urine fertilizer used was derived from fermented Ongole Crossbreed cow urine for 21 days. Fodder sorghum planted for 15 days. The results of the fodder harvest were analyzed by proximate and in vitro nutrient digestibility. Observation nutritional value parameters consists of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), crude fiber (CF), total nutrient digestible (TDN), dry matter digestibility (DMD) and organic matter digestibility (OMD). Data were analyzed using one way ANOVA design, the significance by Duncan's Multiple Range Test.<strong></strong></p><p class="MDPI17abstract"><strong>Results</strong><strong>: </strong>The results showed that the addition of fermented urine had no effect on the DM, OM, and EE, but had a significant effect (P &lt;0.05) on CP, CF, TDN and nutrient digestibility. The T2 and T3 treatments increased crude protein by 6.31% and 11.23% compared to T1. T3 treatment increased organic matter digestibility by 3.06% compared to T1.<strong></strong></p><strong>Conclusions: </strong>It can be concluded that the crude protein and organic matter digestibility of sorghum fodder at 15 days harvest stage, increases with the addition of fermented cattle urine up to 25.0 ml/L hydroponic media.


2019 ◽  
Vol 59 (9) ◽  
pp. 1682
Author(s):  
Anusorn Cherdthong ◽  
Rittikeard Prachumchai ◽  
Chanadol Supapong ◽  
Benjamad Khonkhaeng ◽  
Metha Wanapat ◽  
...  

This experiment was conducted to investigate the utilisation of yeast waste as protein source to replace soybean meal in concentrate mixture on kinetic of gas, rumen ammonia-nitrogen and digestibility of nutrients by using in vitro gas production technique. The experimental design was a completely randomised design and the dietary treatments were replacing soybean meal with yeast wastein concentrate at the ratio of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Yeast waste was obtained from KSL Green Innovation Public Co. Limited, Thailand. The gas production was recorded at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 h of incubation. The yeast waste contained 26.4% crude protein. Gas production from soluble fractions (a), gas production from the insoluble fraction (b), potential extent of gas production (a+b) and the gas production rate constants for the insoluble fraction (c) were not altered when increasing concentration of yeast waste replacing soybean meal (P &gt; 0.05). Cumulative gas production (at 96 h of incubation) ranged from 69.3 to 72.8 mL and was similar among treatments. Ruminal NH3-N concentration was linearly increased (P &lt; 0.05) whereas ruminal pH did not alter when inclusion various levels of yeast waste replacing soybean meal, which ranged from 15.2 to 19.1 mg/dL and 6.90 to 6.94, respectively. In vitro dry matter digestibility and in vitro organic matter digestibility did not changed by increasing levels of yeast waste in the diets (P &gt; 0.05), except only in vitro dry matter digestibility at 12 h, which higher in soybean meal:yeast waste at 25:75 ratio (P &lt; 0.05). Furthermore, propionate (C3) molar was linearly higher when compared between inclusion yeast waste and the control group whereas acetate was decreased quadratically (P &lt; 0.05) and protozoal population tended to be decreased (P = 0.07) when increasing the level of replacing yeast waste. In conclusion, yeast waste could replace soybean meal in concentrate mixture with no negative effect on gas kinetics, rumen fermentation and in vitro digestibility, and therefore its use in animal feeding would contribute to a reduction in environmental pollution.


2013 ◽  
Vol 93 (3) ◽  
pp. 399-402
Author(s):  
J. C. Plaizier ◽  
S. Li

Plaizier, J. C. and Li, S. 2013. Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique. Can. J. Anim. Sci. 93: 399–402. The potential of the gas production technique to predict in vitro dry matter digestibility was determined using 50 ruminant feeds, including grass/alfalfa forages, grain crop silages, grains, high protein feeds, and feed straws. Without including the chemical composition in the prediction equation, the prediction of IVDMD based on the cumulative gas production during 48 h alone was poor (R 2=0.48). Including the crude protein and neutral detergent fibre concentrations of the feed in the prediction equation improved the accuracy of the prediction (R 2=0.87), which was higher than that of predicting IVDMD based on the chemical composition alone (R 2=0.82).


2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Sindu Akhadiarto ◽  
A Fariani

The objective of this research was to study digestibility of kumpai minyak (Hymenachne amplexicaulis) amoniation by in vitro techniques. Materials used in this study were : kumpai oil grass; poultry manure; urea; cattle rumen fluid; Mc buffer solution Dougall; NaOH or H3PO4 and saturated HgCl2. This research was held in two stage, the first was amoniation of kumpai minyak and second was in vitro analysis, both experiment was held at laboratory of Nutrition and Feed Animal, Agriculture Faculty at Sriwijaya University. The research used completely randomized design with 4 treatments and 4 replications which were: A0 (kumpai oil grass without urea), A1 (kumpai oil grass with urea 2%), A2 (kumpai oil grass with urea 4%) and A3 (kumpai oil grass with urea 6%). Parameters measuredwere dry matter digestibility, organic matter digestibility and gas production.. The result showed that the treatment were significantly difference (P<0.01) on organic matter digestibility, but non sinificantly differences (P>0.01) on dry matter digestibility and gas production.


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