Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique

2013 ◽  
Vol 93 (3) ◽  
pp. 399-402
Author(s):  
J. C. Plaizier ◽  
S. Li

Plaizier, J. C. and Li, S. 2013. Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique. Can. J. Anim. Sci. 93: 399–402. The potential of the gas production technique to predict in vitro dry matter digestibility was determined using 50 ruminant feeds, including grass/alfalfa forages, grain crop silages, grains, high protein feeds, and feed straws. Without including the chemical composition in the prediction equation, the prediction of IVDMD based on the cumulative gas production during 48 h alone was poor (R 2=0.48). Including the crude protein and neutral detergent fibre concentrations of the feed in the prediction equation improved the accuracy of the prediction (R 2=0.87), which was higher than that of predicting IVDMD based on the chemical composition alone (R 2=0.82).


Rangifer ◽  
1986 ◽  
Vol 6 (2) ◽  
pp. 8 ◽  
Author(s):  
Torstein H. Garmo

<p>The chemical composition and in vitro dry matter digestibility of 45 samples of different species of lichen are reported. Mean content (g/100 g dry matter) of the main nutrients was: crude protein 4.2, crude fat 3.2, crude fibre 16.6, ash 1.9, Ca 0.15, P 0.09, Mg 0.05, K 0.13, Na 0.035, S 0.07. The content of microminerals (mg/kg dry matter) was: Cu 2.5, Mo 0.11, Zn 27.2, Se 0.12, Fe 898, Mn 154. The mean in vitro dry matter digestibility was 35%. However, the in vitro method do underestimate the dry matter digestibility of lichens. Stereocaulon spp. showed higher levels of crude protein, P, S, Cu and Mo than Cetraria spp. and Cladonia spp. Cetraria nivalis showed higher digestibility and contained more NFE, ash, Ca, Mg, but less crude fibre than Cladonia stellaris. Lichens contained less amounts of most nutrients compared with grasses (Fig. 1), exept for crude fat, NFE, Se and Fe.</p><p>Kjemisk innhald og in vitro ford&oslash;yelsesgrad av lav.</p><p>Abstract in Norwegian / Samandrag: Kjemisk innhald og in vitro ford&oslash;yelsesgrad av t&oslash;rrstoffet er bestemt i 45 pr&oslash;ver av beitelav fr&aring; to stader i S&oslash;r-Noreg. Middel innhald (g/100g t&oslash;rrstoff) av f&oslash;lgjande n&aelig;ringsstoff var: protein 4.2, feitt 3.2, trevlar 16.6, oske 1.9, kalsium 0.15, fosfor 0.09, magnesium 0.05, kalium 0.13, natrium 0.035, svovel 0.07. Innhaldet (mg/kg t&oslash;rrstoff) av mikron&aelig;ringsstoffa var: kopar 2.5, molybden 0.11, sink 27.2, selen 0.12, jern 898 og mangan 154. Den midlare ford&oslash;yelsesgraden av t&oslash;rrstoffet i lav-pr&oslash;vene var 35%, men in vitro ford&oslash;yelsesanalyser undervurderer ford&oslash;yelsesgraden av lav. Det var ein stor variasjon mellom dei ulike lavartane for dei fleste n&aelig;ringsstoffa og ford&oslash;yelsesgraden. Stereocaulon spp. inneheldt meir protein, fosfor, svovel, kopar og molybden enn Cetraria spp. og Cladonia spp. Gulskinn hadde h&oslash;gare ford&oslash;yelsesgrad, og innehaldet av NFE, oske, kalsium og magnesium var h&oslash;gre enn i kvitkrull, medan trevleinnhaldet var st&oslash;rst i kvitkrull. Lav inneheldt mindre av dei fleste n&aelig;ringsstoffa samanlikna med gras, unnateke feitt, NFE, selen og jern.</p><p>J&auml;k&auml;lien kemiallinen sis&auml;lt&ouml; ja in vitro sulatuksen aste.</p><p>Abstract in Finnish / Yhteenveto: Kuiva-aineen kemiallinen sisalto ja in vitro sulatusaste on m&aring;&aring;r&aring;tty 45:ssa kokeessa t&aring;rkeist&aring; laidunj&aring;k&aring;list&aring; kahdella paikkakunnalla Etel&aring; &mdash; Norjassa. Keskim&aring;&aring;r&aring;inen sisalto (g/100 g kuiva-ainetta) seuraavissa ravintoaineissa oli: valkuaisainetta 4.2, rasvaa 3.2, kuituja 16.6, tuhkaa 1.9, kalsiumia 0.15, fosforia 0.09, magnesiumia 0.05, kaliumia 0.13, natriumia 0.035, rikkia 0.07. Hivenainepitoisuus (mg/kg kuiva-ainesta) oli: kuparia 2.5, molybdeenia 0.11, sinkkia 27.2, seleeni&aring; 0.12, rautaa 898 ja mangaania 154. Kuiva-aineiden keskim&aring;&aring;r&aring;inen sulatusaste j&aring;k&aring;l&aring;kokeissa oli 35%. Eri j&aring;k&aring;l&aring;ajien v&aring;lill&aring; oli suuri vaihtelu eisimmissa ravintoaineissa ja sulatusasteessa. Stereocaulon spp. sis&aring;lsi enemm&aring;n valkuaisainetta, fosforia, rikki&aring; kuparia ja typettomien uuteaineiden, molybdeeni&aring; kuin Cetraria spp. ja Cladonia spp. Lapalumij&aring;k&aring;l&aring;ll&aring; oli korkeampi sulatusaste, ja tuhkan, kalsiumin ja magnesiumin sis&aring;lto oli korkeampi kuin Cladonia alpestris-j&auml;k&auml;l&auml;ss&auml;, mutta kuitusis&aring;lto oli korkein Cladonia alpestris-j&auml;k&auml;l&auml;ss&auml;. J&aring;k&aring;l&aring; sis&aring;lsi v&aring;hemm&aring;n useimmista ravintoaineista ruohoon verrattuna lukuunottamatta rasvaa, typettomi&aring; uuteaineita, seleeni&aring; ja rautaa.</p>



2017 ◽  
Vol 39 (3) ◽  
pp. 289 ◽  
Author(s):  
Paula Martins Olivo ◽  
Geraldo Tadeu dos Santos ◽  
Luís Carlos Vinhas Ítavo ◽  
Ranulfo Combuca da Silva Junior ◽  
Eduardo Souza Leal ◽  
...  

Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were tested by analysis of variance using the least square method; the results of gas production were interpreted by a non-linear regression by the Gauss-Newton method; and the effects of treatments were evaluated by the Tukey’s test. The coefficients of in vitro digestibility of dry matter, crude protein and neutral detergent fiber of co-products were different. Gas production was also different between co-products and feeds evaluated for the volume of gas produced from the fast and slow degradation fractions, degradation rate, bacterial colonization time, and the total volume of gas produced. The evaluated co-products exhibited greater in vitro dry matter digestibility compared to corn silage, except for cottonseed, grape residue, and cassava foliage. Co-products showed higher values of in vitro crude protein digestibility compared to corn silage, and a reduced in vitro digestibility of neutral detergent fiber, except for pelleted citrus pulp and soybean hulls. Corn silage produced larger volume of gas from the fast degradation fraction compared to the co-products and corn concentrate. Co-products analyzed had appropriate nutritional characteristics according to the techniques applied and can be included in ruminant diets. 



2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 408-408
Author(s):  
Uchenna Anele ◽  
Sultan Singh ◽  
B P Kushwaha ◽  
P K Gupta ◽  
S Bhattacharya

Abstract Ten types of diets were prepared to meet the nutrient requirements of buffaloes for maintenance (MD1 to MD10), growth (GD1 to GD10) and production/lactation (PD1 to PD10). A total of 30 diets were evaluated for chemical composition, in vitro gas and methane production, and dry matter (DM) disappearance using buffalo rumen liquor. The CNCPS was used to estimate the carbohydrate and protein fractions. Protein fractions PB1 and PB2 of maintenance diets were lower (P &lt; 0.05) than growth and production diets, while protein fractions (PB3) and Pc were (P &lt; 0.05) higher in maintenance than in growth and production diets. Mean values of PA was (P &lt; 0.05) higher in growth diets (136.9) than production (114.8) and maintenance diets (105.6 g/kg DM). Maintenance diets had (P &lt; 0.05) higher structural carbohydrate contents (586.2 g/kg DM) than production diets (513.0 g/kg DM). Carbohydrate fraction (CB1) was highest (P &lt; 0.05) in production diets (187.2 g/kg DM) followed by growth (129.5 g/kg DM) and maintenance diets (96.1 g/kg DM). In vitro gas production at different time periods (12, 24 and 48 h) was similar for maintenance (63.04, 51.98 and 48.15 ml/g DM), growth (63.83, 52.73 and 48.250) and production diets (63.51, 52.54 and 47.21 ml/g DM). Cumulative methane production was numerically lower for maintenance (28.40 ml/g DM) than growth (29.58 ml/g DM) and production diets (33.13 ml/g DM). In vitro methane production as a proportion of degraded DM (ml/g DDM and g/kg DDM) was similar for maintenance (14.21 and 29.53), growth (42.19 and 30.25) and production diets (41.26 and 29.58). Dietary chemical constituents such as EE, lignin, NDIN, ADIN and PB3 and Cc were (P &lt; 0.05) negatively associated with methane production, while OM, NPN, SP, PA and PB1, TCHO and CB2 were positively (P &lt; 0.05) correlated with methane production.



2019 ◽  
Vol 59 (9) ◽  
pp. 1682
Author(s):  
Anusorn Cherdthong ◽  
Rittikeard Prachumchai ◽  
Chanadol Supapong ◽  
Benjamad Khonkhaeng ◽  
Metha Wanapat ◽  
...  

This experiment was conducted to investigate the utilisation of yeast waste as protein source to replace soybean meal in concentrate mixture on kinetic of gas, rumen ammonia-nitrogen and digestibility of nutrients by using in vitro gas production technique. The experimental design was a completely randomised design and the dietary treatments were replacing soybean meal with yeast wastein concentrate at the ratio of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Yeast waste was obtained from KSL Green Innovation Public Co. Limited, Thailand. The gas production was recorded at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 h of incubation. The yeast waste contained 26.4% crude protein. Gas production from soluble fractions (a), gas production from the insoluble fraction (b), potential extent of gas production (a+b) and the gas production rate constants for the insoluble fraction (c) were not altered when increasing concentration of yeast waste replacing soybean meal (P &gt; 0.05). Cumulative gas production (at 96 h of incubation) ranged from 69.3 to 72.8 mL and was similar among treatments. Ruminal NH3-N concentration was linearly increased (P &lt; 0.05) whereas ruminal pH did not alter when inclusion various levels of yeast waste replacing soybean meal, which ranged from 15.2 to 19.1 mg/dL and 6.90 to 6.94, respectively. In vitro dry matter digestibility and in vitro organic matter digestibility did not changed by increasing levels of yeast waste in the diets (P &gt; 0.05), except only in vitro dry matter digestibility at 12 h, which higher in soybean meal:yeast waste at 25:75 ratio (P &lt; 0.05). Furthermore, propionate (C3) molar was linearly higher when compared between inclusion yeast waste and the control group whereas acetate was decreased quadratically (P &lt; 0.05) and protozoal population tended to be decreased (P = 0.07) when increasing the level of replacing yeast waste. In conclusion, yeast waste could replace soybean meal in concentrate mixture with no negative effect on gas kinetics, rumen fermentation and in vitro digestibility, and therefore its use in animal feeding would contribute to a reduction in environmental pollution.



2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Regulo Jimenéz-Guillén ◽  
David Heriberto Noriega Cantú ◽  
Saúl Rojas-Hernández ◽  
Jaime Olivares Pérez ◽  
Abel Villa-Mancera ◽  
...  

Chemical composition and ruminal digestion parameters were mea- sured in the silage of the whole corn plant (Zea mays L.) mixed with mulberry foliage (Morus alba L.) in ratio of 100:0, 80:20, 60:40, 40:60, 20:80 and 00:100% of fresh forage weight, respectively. The 40:60 ratio of corn: mulberry increased crude protein content (CP = 14.28%) and decreased hemicellulose (12.15%), neutral (NDF = 28.00%) and acidic (ADF = 15.85%) detergent fiber, compared to 100: 0% ratio, respectively, which led to a 21.3% increase in in vitro dry matter digestibility (IVDMD). Also ammoniacal nitrogen 7.40% (NH3-N/total N) was higher in the ratio 40:60%, with pH of 4.19 which is normal for silages, which translates the buffer activity correct of ammonia and its importance for silage fermentation stability. The correlation (r = 0.61, p < 0.001) indicated that at higher CP content, higher is IVDMD and hemicellulose (r = -0.99), ADF (r = -0.98) and NDF (r = -0.98) indicated that these fibrous compounds decreased IVDMD (p < 0.001). The regression showed that the increase in content CP (0.555%, p < 0.01) and IVDMD (0.3391%, p < 0.001) with the decrease of NDF (-0.3526%), ADF (-0.1623%) and hemicellulose (-0.1903%) (p < 0.001) in mixed mini silos, was attributed to mulberry foliage addition. It is concluded that the contribution of dry matter and nitrogen of mulberry, decreased the fiber content and increased digestibility parameters of silage when it is elaborated in ratio of 40:60 corn and mulberry, respectively.



2014 ◽  
Vol 22 (4) ◽  
pp. 331-340
Author(s):  
Carlos Augusto Brasileiro de Alencar ◽  
Fernando França da Cunha ◽  
Rubens Alves de Oliveira ◽  
Antônio Carlos Cóser ◽  
Carlos Eugênio Martins

The aim of the study was to evaluate the chemical composition and in vitro dry matter digestibility (IVDMD) of six grasses subjected to different irrigation depths and seasons. The experiment was conducted in a completely randomized arrangement with two replications in a split-split plot design. The treatments included plots with six grasses (Xaraés, Mombaça, Tanzania, Pioneiro, Marandu and Estrela), six irrigation depths for the split-plots (0%, 18%, 45%, 77%, 100% and 120% of the reference value provided by the tensiometer), and two seasons as the split-split plots (autumn/winter and spring/summer). A line source sprinkler system was used for application of the irrigation depths. Crude protein (CP), neutral detergent fiber (NDF) and IVDMD were evaluated and obtained in accordance with literature. The factors studied affect the CP. However in the autumn/winter season the grasses presented differences in the NDF content, where Estrela presented the largest and Pioneiro the smallest NDF contents. The season and irrigation depths did not affect the NDF. Estrela grass presented the smallest IDVMD and the autumn/winter season resulted in greater digestibility for the grasses Xaraés, Mombaça and Tanzania only. The irrigation depths had an effect on some combinations of grasses and seasons.



Author(s):  
K. Sridhar ◽  
C. H. Jagadeesh ◽  
Y. Ramana Reddy ◽  
D. Nagalakshmi ◽  
M. Mahender ◽  
...  

APBN1, a variety of hybrid napier was analysed at five stages of harvest (30, 40, 45, 50 and 60 days) for yield, leaf to stem ratio, chemical composition, in vitro dry matter digestibility, in sacco dry matter and protein degradation to find out optimum stage of harvesting. The yield of green fodder, dry matter (DM) and crude protein (CP) (MT/ ha/year) of forage harvested at 30, 40, 45, 50 and 60 days of growth ranged from 274.68 to 387.95, 47.35 to 98.81, 7.44 to 11.45, respectively. Highest (P less than 0.05) CP yield (11.45 MT/ha) and nitrogen free extract content (46.12%) was observed at 45 d of growth. Crude protein (15.72 to 11.34%), ether extract (3.65 to 2.26%) decreased, whereas total ash (10.88 to 13.86%), crude fibre (23.81 to 29.88%) and acid insoluble ash (3.92 to 5.14%) levels increased when forage was allowed to grow from 30 to 60 d. Similarly, NDF, ADF, hemicellulose, cellulose, lignin and silica levels also increased gradually as plant matures. The in vitro dry matter digestibility was maximum (57.87%) at 30 d and minimum (51.40%) at 60 d of growth. Effective dry matter and protein degradability decreased from 55.80 to 43.78 and from 38.30 to 33.17 per cent, respectively as plant matured from 30 to 60 days. The insoluble but degradable protein fraction ‘b’ was highest (77.07%) at 45 d of growth. Based on the biomass yield, leaf to stem ratio, chemical, in vitro and in sacco evaluation, the optimum stage of harvesting for APBN1 forage was found to be at 45 d of growth.



Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.



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