scholarly journals In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution

2020 ◽  
Vol 24 (1) ◽  
pp. 50
Author(s):  
Rusli Fidriyanto ◽  
Roni Ridwan ◽  
Wulansih Dwi Astuti ◽  
Rohmatussolihat Rohmatussolihat ◽  
Nurul Fitri Sari ◽  
...  
Keyword(s):  
Organic Matter ◽  
Rice Husk ◽  
Rice Bran ◽  
Dry Matter ◽  
Acid Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Fatty Acid Production ◽  
Dry Matter Digestibility

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.

scholarly journals Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1205
Author(s):  
Musen Wang ◽  
Fujin Zhang ◽  
Xinxin Zhang ◽  
Ying Yun ◽  
Lei Wang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nutritional Quality ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Fresh Matter ◽  
In Vitro Rumen Fermentation

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.

scholarly journals Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 59-63
Author(s):  
S.N.O. Suwandyastuti ◽  
Efka Aris Rimbawanto ◽  
Ning Iriyanti
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Straw ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Industrial Wastes ◽  
Optimal Ratio ◽  
Dry Matter Digestibility

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

scholarly journals The Use of Various Substrate Microbial Bamboo Stems for Rice Straw Fermentation and its effect on digestibility and fermentation product of Beef Cattle Ration

2020 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Chabib B.H ◽  
Sri Rahayu ◽  
Muhamad Bata
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Rice Straw ◽  
Rice Bran ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

This experiment investigated various substrate growth of bamboo stems microbes (BSM) in rice straw fermentation and its effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration. Materials were rumen fluid of beef cattle from the slaughterhouse. Basal diets consisted of rice straw and concentrate with ratio of 40 : 60 (% dry matter) were administered to five treatments namely P0 = untreated rice straw (control), P1 = Ammoniated rice straw,  fermented rice straw using microbial bamboo stems grown on ammoniated rice straw (P2), rice bran (P3) and cassava waste (P4). The experiment used in vitro method was designed with Completely Randomized Design and the data were analyzed variance. The results showed that the treatment had no significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration (P>0,05). The fermentation process of microbial bamboo stems in rice straw with a level of 10% with different substrate did not able to  increase the digestibility and products fermentation, but there tends that the rice bran and substrates can increase DMD and N-NH3 concentration

scholarly journals Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Block Design ◽  
Gas Production ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
In Vitro Gas Production

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

scholarly journals KERCERNAAN IN VITRO BAHAN KERING DAN BAHAN ORGANIK SERTA PROTEIN RANSUM BERBASIS PAKAN FERMENTASI

2014 ◽  
Vol 2 (2) ◽  
pp. 70
Author(s):  
Aang Baitul Mizan ◽  
Andi Murlina Tasse ◽  
Deki Zulkarnain
Keyword(s):  
Organic Matter ◽  
Experimental Design ◽  
Rice Bran ◽  
Dry Matter ◽  
Block Design ◽  
Animal Nutrition ◽  
Animal Science ◽  
Dry Matter Digestibility ◽  
Randomized Block Design

This study has conducted four weeks in Laboratory of Animal Nutrition Department of Animal Science, Faculty of Animal Science, Haluoleo University, Kendari. The treatments R0 = rations based feed’s non fermented, R1 = rations based 40% rice bran fermented (RBF), R2 = rations based 45% RBF, R3 = rations based 50% RBF, R4 = rations based 55% RBF, R5 = rations based 60% RBF. The experimental design used a randomized block design (RBD) with 5 treatments and 4 groups. The results showed that dry matter digestibility (DMD) of R1, R2, R5 higher than R0 (94,17%, 90,98%, 91,04% vs 88.60%). In contrast, DMD of R3 and R4 lower than R0 (80,90% and 80,89% vs 88,60%). Organic matter digestibility (OMD) of R0 lower than R1, R2, R3, R4 and R5 ( 62,58% vs 90,32%, 90,55%, 90,78%, 91,15% and 90,41%). In contrast, OMD of R4 higher than R1, R2, R3, R5 and R0 (91,41% vs 90,32%, 90,55%, 90,78%, 90,41% and 62,58%). Ammonia (NH3) concentrations of R1, R2, R3, R4 and R5 higher than R0 (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM and 7,42 mM vs 3,20 mM). The conclusion of this study was FBR based 40%, 45%, 50%, 55% and 60% RBF feasible to ruminant’s feed.Keywords : Feed’s Fermented, Digestibility, Dry Matter, Organic Matter, Ammonia.ABSTRAKPenelitian ini bertujuan mengevaluasi kelayakan penggunaan ransum berbasis pakan fermentasi untuk ternak ruminansia berdasarkan kecernaan bahan kering dan bahan orgaik serta protein secara In Vitro. Penelitian ini dilaksanakan selama empat minggu di Laboratorium Ilmu Nutrisi dan Makanan Ternak Fakultas Peternakan Universitas Haluoleo, Kendari. Perlakuan yang dicobakan adalah R0= ransum berbasis pakan non fermentasi (kontrol), R1= ransum berbasis dedak padi fermentasi 40%, R2= ransum berbasis dedak padi fermentasi 45%, R3= ransum berbasis dedak padi fermentasi 50%, R4= ransum berbasis dedak padi fermentasi 55%, R5= ransum berbasis dedak padi fermentasi 60%. Rancangan percobaan yang digunakan rancangan acak kelompok (RAK) dengan 5 perlakuan dan 4 kelompok. Hasil analisis ragam menunjukkan kecernaan bahan kering ransum berbasi dedak padi fermentasi 40%, 45%, dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (94,17%; 90,98% dan 91,04% dibanding dengan 88,60%). Sebaliknya, ransum berbasis dedak padi fermentasi 50% dan 55% lebih rendah dibanding dengan ransum berbasis pakan non fermentasi (80,90% dan 80,89% dibanding dengan 88,60%). Kecernaan bahan organik ransum berbasis pakan non fermentasi lebih rendah dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% (62,58 % dibanding dengan 90,32 %, 90,55 %, 90,78 %, 91,15 % dan 90,41 % ). Sebaliknya kecernaan bahan organik ransum berbasis dedak padi fermentasi 55% lebih tinggi dibanding dengan ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 60% dan kontrol (91,41% dibanding dengan 90,32%, 90,55%, 90,78%, 90,41% dan 62,58%). Konsentrasi amonia ransum berbasis dedak padi fermentasi 40%, 45%, 50%, 55% dan 60% lebih tinggi dibanding dengan ransum berbasis pakan non fermentasi (8,20 mM, 5,40 mM, 8,67 mM, 7,22 mM dan 7,42 mM dibanding dengan 3,20 mM). Kesimpulan bahwa ransum berbasis dedak padi fermentasi 40% hingga 60% layak digunakan untuk pakan ternak ruminansia.Kata kunci: Pakan Fermentasi, Kecernaan Bahan Kering, Kecernaan Bahan Organik, Amonia.

scholarly journals Effects of tannins and Monensin in a feedlot diet on in vitro ruminal fermentation

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 3223
Author(s):  
Hariany Ferreira Martello ◽  
Nelcino Francisco de Paula ◽  
Bruna Gomes Macedo ◽  
Joanis Tilemahos Zervoudakis ◽  
Danielle Dias Brutti ◽  
...  
Keyword(s):  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Low Dose ◽  
Ammonia Concentration ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Ruminal Fermentation ◽  
Kinetics Parameters ◽  
Dry Matter Digestibility

The objective of this study was to evaluate the effects of tannins versus Monensin on in vitro ruminal fermentation of a feedlot diet. The treatments were: control (no additives); low tannin (2 mg g DM-1); medium tannin (4 mg g DM-1), high tannin (6 mg g DM-1), and Monensin (0.02 mg g DM-1). The substrate was a feedlot diet composed by hay and concentrate (15:85 w/w; DM basis). Ruminal fluid was obtained from three rumen-cannulated male Santa Inês sheep. In vitro incubations were carried out during four consecutive weeks (run). Gas production (GP) was recorded at 1, 2, 3, 4, 5, 6, 8, 10, 12, 18, 24, 30, 36, 42, 48, 60, 72, 84, and 96 h of incubation. At 48 and 96 h, two bottles per treatment were withdrawn to measure pH, ammonia concentration (NH3), volatile fatty acid (VFA), in vitro dry matter digestibility (IVDMD), and in vitro neutral detergent fiber digestibility (IVNDFD). Addition of tannin or Monensin did not affect (P > 0.05) the kinetics parameters. Tannin supplementation reduced (P < 0.05) the GP at 24 h compared to Monensin. Addition of Monensin decreased (P < 0.05) IVDMD at 96 h and IVNDFD at 48 and 96 h compared to the control. The IVNDFD was lower (P < 0.05) with Monensin than with tannin at 48 and 96 h. The NH3 was lower (P < 0.05) with tannin compared with Monensin. By increasing tannin dosage, NH3 levels changed quadratically (P < 0.05). The inclusion of tannin in vitro reduced the NH3 concentration considerably when used in low dose.

scholarly journals The Effect of Zinc-Proteinate Supplementation on the In Vitro Digestibility and Ruminal Fermentation in Goat

2021 ◽  
pp. 180-186
Author(s):  
Gilbert Nathaniel ◽  
Tiara Annisa ◽  
Anis Muktiani ◽  
Dian Wahyu Harjanti ◽  
Widiyanto Widiyanto
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Experimental Treatment ◽  
Dairy Goat ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

This study aimed primarily to investigate the effect of Zn-Proteinate (Zn-Prot) supplementation on in vitro rumen digestibility and rumen fermentation. This research used a completely randomized design with four treatments and four replicates. The experimental treatment was the supplementation of various levels of Zn-Prot (0; 12.5 ppm; 25 ppm, and 37.5 ppm) on a dry matter basis. Parameters determined were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), partial volatile fatty acid (VFA) (acetate, propionate, and butyrate), A/P ratio, CH4, and the efficiency of the conversion hexose to VFA. Data were analyzed using ANOVA. The results showed that goats fed with a diet supplemented with 25 ppm Zn-Prot had the highest IVDMD and IVOMD values. There was no significant effect on the VFA and CH4 concentrations, A/P ratio, and the efficiency of the hexose-VFA conversion within treatment groups. In conclusion, supplementing 25 ppm of Zn-Prot into the diet of dairy goat increase the dry matter and organic matter digestibility. 

EVALUASI KECERNAAN RUMPUT KUMPAI MINYAK (Hymenachne amplexicaulis) AMONIASI SECARA IN VITRO

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Sindu Akhadiarto ◽  
A Fariani
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Poultry Manure ◽  
Gas Production ◽  
Buffer Solution ◽  
In Vitro Techniques ◽  
Dry Matter Digestibility ◽  
Organic Matter Digestibility

The objective of this research was to study digestibility of kumpai minyak (Hymenachne amplexicaulis) amoniation by in vitro techniques. Materials used in this study were : kumpai oil grass; poultry manure; urea; cattle rumen fluid; Mc buffer solution Dougall; NaOH or H3PO4 and saturated HgCl2. This research was held in two stage, the first was amoniation of kumpai minyak and second was in vitro analysis, both experiment was held at laboratory of Nutrition and Feed Animal, Agriculture Faculty at Sriwijaya University. The research used completely randomized design with 4 treatments and 4 replications which were: A0 (kumpai oil grass without urea), A1 (kumpai oil grass with urea 2%), A2 (kumpai oil grass with urea 4%) and A3 (kumpai oil grass with urea 6%). Parameters measuredwere dry matter digestibility, organic matter digestibility and gas production.. The result showed that the treatment were significantly difference (P<0.01) on organic matter digestibility, but non sinificantly differences (P>0.01) on dry matter digestibility and gas production.

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