scholarly journals COR/LEA Proteins as Indicators of Frost Tolerance in Triticeae: A Comparison of Controlled Versus Field Conditions

2021 ◽  
Author(s):  
Klára Kosová ◽  
Miroslav Klíma ◽  
Ilja Tom Prášil ◽  
Pavel Vítámvás
Keyword(s):  
Cold Acclimation ◽  
Field Experiments ◽  
Quantitative Relationship ◽  
Field Trials ◽  
Frost Tolerance ◽  
Late Embryogenesis Abundant ◽  
Field Conditions ◽  
Lea Proteins ◽  
Winter Cereals ◽  
Enhanced Expression

Low temperatures in the autumn induce enhanced expression/relative accumulation of several cold-inducible transcripts/proteins with protective functions from Late-embryogenesis-abundant (LEA) superfamily including dehydrins. Several studies dealing with plants grown under controlled conditions revealed a correlation (significant quantitative relationship) between dehydrin transcript/protein relative accumulation and plant frost tolerance. However, to apply these results in breeding, field experiments are necessary. The aim of the review is to provide a summary of the studies dealing with the relationships between plant acquired frost tolerance and COR/LEA transcripts/proteins relative accumulation in cereals grown in controlled and in the field conditions. The impacts of cold acclimation and vernalization processes on the ability of winter-type Triticeae to accumulate COR/LEA proteins are discussed. The factors determining dehydrin relative accumulation under controlled cold acclimation treatments versus field trials during winter seasons are discussed. In conclusion, it can be stated that dehydrins could be used as suitable indicators of winter surival in field-grown winter cereals but only in plant prior to the fullfilment of vernalization requirement.

scholarly journals COR/LEA Proteins as Indicators of Frost Tolerance in Triticeae: A Comparison of Controlled versus Field Conditions

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 789
Author(s):  
Klára Kosová ◽  
Miroslav Klíma ◽  
Ilja Tom Prášil ◽  
Pavel Vítámvás
Keyword(s):  
Cold Acclimation ◽  
Field Experiments ◽  
Quantitative Relationship ◽  
Field Trials ◽  
Frost Tolerance ◽  
Late Embryogenesis Abundant ◽  
Field Conditions ◽  
Lea Proteins ◽  
Winter Cereals ◽  
Vernalisation Requirement

Low temperatures in the autumn induce enhanced expression/relative accumulation of several cold-inducible transcripts/proteins with protective functions from Late-embryogenesis-abundant (LEA) superfamily including dehydrins. Several studies dealing with plants grown under controlled conditions revealed a correlation (significant quantitative relationship) between dehydrin transcript/protein relative accumulation and plant frost tolerance. However, to apply these results in breeding, field experiments are necessary. The aim of the review is to provide a summary of the studies dealing with the relationships between plant acquired frost tolerance and COR/LEA transcripts/proteins relative accumulation in cereals grown in controlled and field conditions. The impacts of cold acclimation and vernalisation processes on the ability of winter-type Triticeae to accumulate COR/LEA proteins are discussed. The factors determining dehydrin relative accumulation under controlled cold acclimation treatments versus field trials during winter seasons are discussed. In conclusion, it can be stated that dehydrins could be used as suitable indicators of winter survival in field-grown winter cereals but only in plant prior to the fulfilment of vernalisation requirement.

scholarly journals Synergistic effect of naturally occurring Granulosis virus isolates (PbGV) with phagostimulants against the cabbage butterfly, Pieris brassicae (L.) for its eco-friendly management

2022 ◽  
Vol 32 (1) ◽  
Author(s):  
Ramandeep Kour ◽  
R. K. Gupta ◽  
Barkat Hussain ◽  
Simranjeet Kour
Keyword(s):  
Field Experiments ◽  
Field Trials ◽  
Pieris Brassicae ◽  
Field Conditions ◽  
Leafy Vegetables ◽  
Granulosis Virus ◽  
Larval Population ◽  
Cabbage Butterfly ◽  
Combined Application ◽  
Naturally Occurring

Abstract Background To manage the cabbage butterfly, Pierisbrassicae (L.) (Lepidoptera: Pieridae), it is not wise to use insecticides on leafy vegetables which are eaten mostly fresh. During the past decades, the efforts to manage the pest, through chemical insecticides have raised serious health. Investigations were carried out to isolate naturally occurring GVs (PbGV) as a potent biopesticide against P.brassicae and to explore their efficacy with the application of phagostimulants. Results Among the four naturally occurring isolates obtained from Northwestern Himalayas, Sudhmahadev isolate was found to be the most promising based on virulence and speed of kill against all the instars tested in the laboratory, showing the natural incidence of PbGV infection in field conditions. In concentration and time–response bioassay, all the isolates of P.brassicae Granulosis virus were found high virulent against second instar larvae of cabbage butterfly. Therefore, for enhanced efficacy of PBGV, its combined application with phagostimulants (Lepidiumsativum + Teepol + jaggery) or sticker (Teepol + jaggery), applied in field trials, resulted into greater mortality of larval instars than the single one. Overall, the results indicated that the introduction of a more isolates PBGV strain into populations of P.brassicae could be of vital importance for eco-friendly suppression of this pest globally with the combination of phagostimulants. The application virus alone with the pre-standardized concentration of 1 × 1012 OBs/ha did not reduce the larval population density to the desirable extent in the greenhouse chamber and therefore was not included in field experiments. Overall, the most promising treatments in reducing the larval population of the pest were PbGV + Teepol + B.thuringiensis (93.49 and 91.39%) and PbGV + Teepol + L.sativum (88.79 and 86.97%) over control in both greenhouse and field trials, respectively. Conclusions In this study, the native isolates of PbGV from different target locations to test their efficacy against different instars of P.brassicae were explored. Using native PBGV isolates with phagostimulant combinations played an important role for regulating the pest effectively. These phagostimulants not only protected the OBs from degradation in the presence of sunlight but also increased the speed of killing. The biocontrol potential of PbGV in both laboratory and field conditions indicated that baculoviruses are sustainable alternative to chemical insecticides.

Effect of Water Depth on the Infection of Biomphalaria glabrata by Miracidia of St. Lucian Schistosoma mansoni under Laboratory and Field Conditions

1972 ◽  
Vol 46 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Edward Suchart Upatham
Keyword(s):  
Schistosoma Mansoni ◽  
Water Depth ◽  
Water Surface ◽  
Field Experiments ◽  
Biomphalaria Glabrata ◽  
Field Trials ◽  
Field Conditions ◽  
Water Volume ◽  
Infection Rates ◽  
Point Of Entry

1. Laboratory and field trials were carried out to investigate the influence of depth on the infection of B. glabrata by St. Lucian S. mansoni miracidia.2. In both laboratory and field experiments, miracidia located and infected snails to a depth of 122 cm.3. The degree of infection seems to be related to the size of a habitat and its water volume.4. Infection rates were high at the miracidial point of entry where miracidium-snail interaction was maximum and at the water surface where miracidia eventually congregated.

scholarly journals Characterization of the Heat-Stable Proteome During Seed Germination in Arabidopsis with Special Focus on LEA Proteins

2021 ◽  
Vol 22 (15) ◽  
pp. 8172
Author(s):  
Orarat Ginsawaeng ◽  
Michal Gorka ◽  
Alexander Erban ◽  
Carolin Heise ◽  
Franziska Brueckner ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Establishment ◽  
Intrinsically Disordered Proteins ◽  
Late Embryogenesis Abundant ◽  
Disordered Proteins ◽  
Similar Proportion ◽  
Special Focus ◽  
Lea Proteins ◽  
Intrinsically Disordered ◽  
Heat Stable

During seed germination, desiccation tolerance is lost in the radicle with progressing radicle protrusion and seedling establishment. This process is accompanied by comprehensive changes in the metabolome and proteome. Germination of Arabidopsis seeds was investigated over 72 h with special focus on the heat-stable proteome including late embryogenesis abundant (LEA) proteins together with changes in primary metabolites. Six metabolites in dry seeds known to be important for seed longevity decreased during germination and seedling establishment, while all other metabolites increased simultaneously with activation of growth and development. Thermo-stable proteins were associated with a multitude of biological processes. In the heat-stable proteome, a relatively similar proportion of fully ordered and fully intrinsically disordered proteins (IDP) was discovered. Highly disordered proteins were found to be associated with functional categories development, protein, RNA and stress. As expected, the majority of LEA proteins decreased during germination and seedling establishment. However, four germination-specific dehydrins were identified, not present in dry seeds. A network analysis of proteins, metabolites and amino acids generated during the course of germination revealed a highly connected LEA protein network.

scholarly journals Exploiting Thrips Aggregation Pheromones to Develop a Lure-and-Kill Strategy for the Management of the Bean Flower Thrips

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1269
Author(s):  
David K. Mfuti ◽  
Amanuel Tamiru ◽  
William D. J. Kirk ◽  
Adeyemi O. Akinyemi ◽  
Heather Campbell ◽  
...  
Keyword(s):  
Field Experiments ◽  
Insect Pests ◽  
Field Trials ◽  
Aggregation Pheromones ◽  
Megalurothrips Sjostedti ◽  
Flower Thrips ◽  
Antifungal Effects ◽  
Field Efficacy ◽  
Management Alternative ◽  
Trap Catches

The potential of semiochemicals to lure insect pests to a trap where they can be killed with biopesticides has been demonstrated as an eco-friendly pest management alternative. In this study, we tested two recently characterized male-produced aggregation pheromones of the bean flower thrips Megalurothrips sjostedti (Trybom), namely (R)-lavandulyl 3-methylbutanoate (major) and (R)-lavandulol (minor), for their field efficacy. Moreover, compatibility of these pheromones and two other thrips attractants, Lurem-TR and neryl (S)-2-methylbutanoate, with the entomopathogenic fungus (EPF) Metarhizium anisopliae ICIPE 69 has been determined. Our study revealed that the M. sjostedti aggregation pheromones have dose-dependent antifungal effects on the EPF viability, but showed no fungistatic effect at a field-realistic dose for attraction of thrips. (R)-lavandulyl 3-methylbutanoate had similar antifungal effects as neryl (S)-2-methylbutanoate 8 days after exposure; whereas, Lurem-TR had a stronger antifungal effect than other thrips attractants. In the semi-field experiments, all autoinoculation devices maintained at least 86% viability of M. anisopliae conidia after 12 days of exposure. Field trials demonstrated for the first time that (R)-lavandulyl 3-methylbutanoate increases trap catches. Our findings pave a way for designing a lure-and-kill thrips management strategy to control bean flower thrips using autoinoculation devices or spot spray application.

scholarly journals Synergistic Effect of Combined Application of a New Fungicide Fluopimomide with a Biocontrol Agent Bacillus methylotrophicus TA-1 for Management of Gray Mold in Tomato

Plant Disease ◽  
2019 ◽  
Vol 103 (8) ◽  
pp. 1991-1997 ◽  
Author(s):  
Xiaoxue Ji ◽  
Jingjing Li ◽  
Zhen Meng ◽  
Shouan Zhang ◽  
Bei Dong ◽  
...  
Keyword(s):  
Field Experiments ◽  
Biocontrol Agent ◽  
Severe Disease ◽  
Combined Treatment ◽  
Field Trials ◽  
Antimicrobial Activities ◽  
Gray Mold ◽  
Control Efficacy ◽  
Bacillus Methylotrophicus

Gray mold caused by Botrytis cinerea can be a severe disease of tomato infecting leaves and fruits of tomato plants. Chemical control is currently the most effective and reliable method; however, application of fungicides has many drawbacks. The combination of biological control agents with newly developed fungicides may be a practicable method to control B. cinerea. Fluopimomide is a newly developed fungicide with a novel mode of action. Bacillus methylotrophicus TA-1, isolated from rhizosphere soil of tomato, is a bacterial strain with a broad spectrum of antimicrobial activities. Little information is currently available about the effect of fluopimomide and its integrated effect on B. cinerea. Therefore, laboratory, pot, and field experiments were carried out to determine the effects of fluopimomide alone and in combination with B. methylotrophicus TA-1 against gray mold on tomato. The in vitro growth of B. methylotrophicus TA-1 was unaffected by 100 mg liter−1 fluopimomide. Inhibition of B. cinerea mycelial growth was significantly increased under combined treatment of fluopimomide and B. methylotrophicus TA-1. In greenhouse experiments, efficacy against gray mold was significantly greater by an integration of fluopimomide and B. methylotrophicus TA-1 than by either alone; control efficacy of fluopimomide at 50 and 100 g ha−1 in combination with B. methylotrophicus TA-1 at 108 colony-forming units (cfu) ml−1 reached 70.16 and 69.32%, respectively, compared with the untreated control. In both field trials during 2017 and 2018, control efficacy was significantly higher for the combination of fluopimomide at 50 and 100 g ha−1 in combination with B. methylotrophicus TA-1 than for either treatment alone. The results from this study indicated that integration of the new fungicide fluopimomide with the biocontrol agent B. methylotrophicus TA-1 synergistically increased control efficacy of the fungicide against gray mold of tomato.

Factors affecting the yield of winter cereals in crop margins

2000 ◽  
Vol 135 (4) ◽  
pp. 335-346 ◽  
Author(s):  
A. WILCOX ◽  
N. H. PERRY ◽  
N. D. BOATMAN ◽  
K. CHANEY
Keyword(s):  
Soil Compaction ◽  
Dry Matter ◽  
Field Experiments ◽  
Polynomial Regression ◽  
Negative Relationship ◽  
Fertilizer Application ◽  
Winter Cereal ◽  
Edge Field ◽  
Winter Cereals ◽  
Non Linear

Yields of arable crops are commonly lower on the crop margins or headlands, but the nature of the relationship between yield and distance from the crop edge has not been clearly defined, nor have the reasons for lower marginal yields. Surveys of 40 winter wheat headlands were carried out in 2 years to determine how yield changed with distance, and what factors might influence this relationship. Two field experiments were also conducted over 3 years in winter cereal headlands, in which the effect of distance was measured under conservation headland and conventional (fully sprayed) management.Yields in the headland surveys varied from 0·8 to 10·2 t/ha. An inverse polynomial regression model was fitted to yield and weed data. Best fits were obtained by using separate parameters for each site. Adjusting yields to take account of weed dry matter improved the non-linear fit between yield and distance from crop edge. Field experiments provided similar results but the non-linear relationship was not as apparent.There was a negative relationship between soil compaction, as measured by a cone penetrometer, and yield in one field experiment, where soil density values were relatively constant. No relationship was found between pattern of nitrogen fertilizer application and yield. Conservation headland management resulted in lower yield at one experimental site, especially in the third year, but not at the other site. Where yields were affected, weed dry matter was higher in conservation headland plots than in fully sprayed plots.Although greater weed competition appears to account for at least part of the observed yield reductions on headlands, the role of other factors, particularly soil compaction, needs further study. Increased weed infestation may be an indirect result of reduced crop competition caused by other adverse conditions.

FACTORS AFFECTING ADULT EMERGENCE AND MATING BEHAVIOR OF THE EASTERN SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1975 ◽  
Vol 107 (9) ◽  
pp. 967-977 ◽  
Author(s):  
C. J. Sanders
Keyword(s):  
Field Experiments ◽  
Choristoneura Fumiferana ◽  
Adult Emergence ◽  
Spruce Budworm ◽  
Field Conditions ◽  
Continuous Illumination ◽  
Degree Days ◽  
Factors Affecting ◽  
Eastern Spruce Budworm ◽  
Duration Of Copulation

AbstractLaboratory and field experiments indicate that the female spruce budworm (Choristoneura fumiferana (Clem.)) pupal stadium requires approximately 122C degree-days above a threshold of 7.2 °C (45°F), the male 124. Emergence time on any given day depends on temperature but is independent of photoperiod. Under field conditions male and female budworm mate only once per 24-h period. In the laboratory under continuous illumination females mate repeatedly and males readily mate a second time within a few hours, but the duration of the second copulation is abnormally long. The probability of multiple matings under field conditions is reduced by the restricted period of sexual activity coupled with the duration of copulation and the lower competitiveness of mated insects. Antennae are essential to the male for successful copulation.

Effects of irrigation regimes on powdery scab disease and yield of potatoes

1986 ◽  
Vol 26 (6) ◽  
pp. 745 ◽  
Author(s):  
PA Taylor ◽  
SP Flett ◽  
RFde Boer ◽  
D Marshall
Keyword(s):  
Large Scale ◽  
Field Experiments ◽  
Field Trials ◽  
Powdery Scab ◽  
Infested Soil ◽  
Spongospora Subterranea ◽  
Potato Tubers ◽  
Potato Plants ◽  
Tuber Set ◽  
Large Scale Field

The period of susceptibility of potato tubers to powdery scab (Spongospora subterranea) was studied by inoculating potato plants with spores, or by watering plants in infested soil, at different stages of plant development in greenhouse conditions. Maximum susceptibility began about 1 week before the stage when 50% of stolons had swollen to at least 5-mm diameter (tuber set), and ended 3-4 weeks later. With holding irrigation water during this period reduced the severity of powdery scab by 65-75% in field experiments in 1981-82 and 1982-83, but had no apparent effect on disease severity in 3 out of 6 large-scale field trials during 1984-85.

Management strategies to reduce losses caused by fusarium seedling blight of field pea

2013 ◽  
Vol 93 (4) ◽  
pp. 619-625 ◽  
Author(s):  
K. F. Chang ◽  
S. F. Hwang ◽  
H. U. Ahmed ◽  
B. D. Gossen ◽  
G. D. Turnbull ◽  
...  
Keyword(s):  
Root Rot ◽  
Field Experiments ◽  
Management Strategies ◽  
Stand Density ◽  
Field Trials ◽  
Field Pea ◽  
Seedling Blight ◽  
Inoculum Level ◽  
Fusarium Seedling Blight ◽  
Disease Pressure

Chang, K. F., Hwang, S. F., Ahmed, H. U., Gossen, B. D., Turnbull, G. D. and Strelkov, S. E. 2013. Management strategies to reduce losses caused by fusarium seedling blight of field pea. Can. J. Plant Sci. 93: 619–625. Fusarium seedling blight can cause substantial reductions in the stand density of field pea in western Canada. In greenhouse experiments, emergence decreased and root rot severity rose with increasing inoculum density. In field trials in 2007 and 2008 near Edmonton, AB, seeding at different depths and seeding dates did not consistently affect emergence or yield in Fusarium-infested soils. In field experiments, emergence declined significantly with each increase in inoculum level. Also, seed yield were reduced at high levels of disease pressure. Treatment of seed with Apron Maxx improved emergence, nodulation and yield of treatments challenged with inoculum of F. avenaceum in both greenhouse and field experiments. This research demonstrates the need to prevent seedling blight and root rot through proper seed treatment.

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