scholarly journals A Portable Device for LAMP Based Detection of Sars-Cov-2

2021 ◽  
Author(s):  
Kamalalayam Rajan Sreejith ◽  
Muhammad Umer ◽  
Narshone Soda ◽  
Surasak Kasetsirikul ◽  
Muhammad Shiddiky ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Rapid Detection ◽  
Copy Number ◽  
Patient Management ◽  
Low Cost ◽  
Portable Device ◽  
Design Development ◽  
Isothermal Heating ◽  
Template Copy ◽  
Cartridge Heater

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 minutes using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard qPCR machine (~10-20 minutes vs >38 minutes) for 1×105 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need SARS-CoV-2 detection and patient management.

scholarly journals A Portable Device for LAMP Based Detection of SARS-CoV-2

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1151
Author(s):  
Kamalalayam Rajan Sreejith ◽  
Muhammad Umer ◽  
Larissa Dirr ◽  
Benjamin Bailly ◽  
Patrice Guillon ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Copy Number ◽  
Low Cost ◽  
Quantitative Polymerase Chain Reaction ◽  
Portable Device ◽  
Design Development ◽  
Isothermal Heating ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Template Copy

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

scholarly journals Sunflower-Like Nanostructure with Built-In Hotspots for Alpha-Fetoprotein Detection

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1197
Author(s):  
Xiaoyu Zhao ◽  
Aonan Zhu ◽  
Yaxin Wang ◽  
Yongjun Zhang ◽  
Xiaolong Zhang
Keyword(s):  
Rapid Detection ◽  
Clinical Medicine ◽  
Electromagnetic Coupling ◽  
Low Cost ◽  
Surface Enhanced Raman Spectroscopy ◽  
Alpha Fetoprotein ◽  
Low Concentrations ◽  
Surface Enhanced Raman ◽  
Active Substrate ◽  
Array Structures

In the present study, a sunflower-like nanostructure array composed of a series of synaptic nanoparticles and nanospheres was manufactured through an efficient and low-cost colloidal lithography technique. The primary electromagnetic field contribution generated by the synaptic nanoparticles of the surface array structures was also determined by a finite-difference time-domain software to simulate the hotspots. This structure exhibited high repeatability and excellent sensitivity; hence, it was used as a surface-enhanced Raman spectroscopy (SERS) active substrate to achieve a rapid detection of ultra-low concentrations of Alpha-fetoprotein (AFP). This study demonstrates the design of a plasmonic structure with strong electromagnetic coupling, which can be used for the rapid detection of AFP concentration in clinical medicine.

Design, Development and Application of Vehicle Gas Turbine Engines

1966 ◽  
Vol 181 (1) ◽  
pp. 149-172
Author(s):  
P. A. Phillips ◽  
Peter Spear
Keyword(s):  
Gas Turbine ◽  
Gas Turbines ◽  
Low Cost ◽  
Engine Performance ◽  
Turbine Engines ◽  
Gas Turbine Engines ◽  
Design Development ◽  
Turbine Engine ◽  
Wide Range ◽  
Cycle Temperature

After briefly summarizing worldwide automotive gas turbine activity, the paper analyses the power plant requirements of a wide range of vehicle applications in order to formulate the design criteria for acceptable vehicle gas turbines. Ample data are available on the thermodynamic merits of various gas turbine cycles; however, the low cost of its piston engine competitor tends to eliminate all but the simplest cycles from vehicle gas turbine considerations. In order to improve the part load fuel economy, some complexity is inevitable, but this is limited to the addition of a glass ceramic regenerator in the 150 b.h.p. engine which is described in some detail. The alternative further complications necessary to achieve satisfactory vehicle response at various power/weight ratios are examined. Further improvement in engine performance will come by increasing the maximum cycle temperature. This can be achieved at lower cost by the extension of the use of ceramics. The paper is intended to stimulate the design application of the gas turbine engine.

A low-cost and portable device for measuring spectrum of light source as a stimulus for the human’s circadian system

2021 ◽  
Vol 252 ◽  
pp. 111386
Author(s):  
Armin Amirazar ◽  
Mona Azarbayjani ◽  
Maziyar Molavi ◽  
Morteza Karami
Keyword(s):  
Light Source ◽  
Low Cost ◽  
Circadian System ◽  
Portable Device

Application of nanomaterials in microbial-cell biosensor constructions

2015 ◽  
Vol 69 (1) ◽  
Author(s):  
Jana Šefčovičová ◽  
Jan Tkac
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Rapid Detection ◽  
Food Industry ◽  
Low Cost ◽  
Microbial Cell ◽  
Direct Connection ◽  
Qualitative Detection ◽  
Analytical Tools ◽  
Cell Biosensor

AbstractMicrobial cell biosensors, where cells are in direct connection with a transducer enabling quantitative and qualitative detection of an analyte, are very promising analytical tools applied mainly for assays in the environmental field, food industry or biomedicine. Microbial cell biosensors are an excellent alternative to conventional analytical methods due to their specificity, rapid detection and low cost of analysis. Nowadays, nanomaterials are often used in the construction of biosensors to improve their sensitivity and stability. In this review, the combination of microbial and other individual cells with different nanomaterials (carbon nanotubes, graphene, gold nanoparticles, etc.) for the construction of biosensors is described and their applications are provided as well.

scholarly journals Quantitative Analysis of Fluorescence Detection Using a Smartphone Camera for a PCR Chip

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3917
Author(s):  
Jong-Dae Kim ◽  
Chan-Young Park ◽  
Yu-Seop Kim ◽  
Ji-Soo Hwang
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Detection ◽  
High Performance ◽  
Low Cost ◽  
Dna Amplification ◽  
Fluorescent Detection ◽  
Rt Pcr ◽  
Commercial System ◽  
Intensity Changes ◽  
The Difference

Most existing commercial real-time polymerase chain reaction (RT-PCR) instruments are bulky because they contain expensive fluorescent detection sensors or complex optical structures. In this paper, we propose an RT-PCR system using a camera module for smartphones that is an ultra small, high-performance and low-cost sensor for fluorescence detection. The proposed system provides stable DNA amplification. A quantitative analysis of fluorescence intensity changes shows the camera’s performance compared with that of commercial instruments. Changes in the performance between the experiments and the sets were also observed based on the threshold cycle values in a commercial RT-PCR system. The overall difference in the measured threshold cycles between the commercial system and the proposed camera was only 0.76 cycles, verifying the performance of the proposed system. The set calibration even reduced the difference to 0.41 cycles, which was less than the experimental variation in the commercial system, and there was no difference in performance.

Development of a genomic predictive model for cholangiocarcinoma using copy number alteration data

2021 ◽  
pp. jclinpath-2020-207346
Author(s):  
Inês Tavares ◽  
Ricardo Martins ◽  
Ilda Patrícia Ribeiro ◽  
Luísa Esteves ◽  
Francisco Caramelo ◽  
...  
Keyword(s):  
Survival Analysis ◽  
Copy Number ◽  
Patient Management ◽  
Copy Number Alteration ◽  
Comparative Genomic Hybridisation ◽  
Support Vector ◽  
Comparative Genomic ◽  
Support Vector Machine Algorithm ◽  
Rare Tumour ◽  
Genomic Model

AimsCholangiocarcinoma (CC) is a rare tumour arising from the biliary tract epithelium. The aim of this study was to perform a genomic characterisation of CC tumours and to implement a model to differentiate extrahepatic (ECC) and intrahepatic (ICC) cholangiocarcinoma.MethodsDNA extracted from tumour samples of 23 patients with CC, namely 10 patients with ECC and 13 patients with ICC, was analysed by array comparative genomic hybridisation. A support vector machine algorithm for classification was applied to the genomic data to distinguish between ICC and ECC. A survival analysis comparing both groups of patients was also performed.ResultsWith these whole genome results, we observed several common alterations between tumour samples of the same CC anatomical type, namely gain of Xp and loss of 3p, 11q11, 14q, 16q, Yp and Yq in ICC tumours, and gain of 16p25.3 and loss of 3q26.1, 6p25.3–22.3, 12p13.31, 17p, 18q and Yp in ECC tumours. Gain of 2q37.3 was observed in the samples of both tumour subtypes, ICC and ECC. The developed genomic model comprised four chromosomal regions that seem to enable the distinction between ICC and ECC, with an accuracy of 71.43% (95% CI 43% to 100%). Survival analysis revealed that in our cohort, patients with ECC survived on average 8 months less than patients with ICC.ConclusionsThis genomic characterisation and the introduction of genomic models to clinical practice could be important for patient management and for the development of targeted therapies. The power of this genomic model should be evaluated in other CC populations.

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