scholarly journals Extracellular Vesicles in Skin Wound Healing

2021 ◽  
Author(s):  
Deimantė Narauskaitė ◽  
Gabrielė Vydmantaitė ◽  
Justina Rusteikaitė ◽  
Revathi Sampath ◽  
Akvilė Rudaitytė ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Current Knowledge ◽  
Skin Wound ◽  
Surface Proteins ◽  
Therapeutic Interventions ◽  
Skin Wound Healing ◽  
Skin Repair ◽  
Healing Wound ◽  
Healing Phase

Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signalling takes part in all four wound healing phases: hemostasis, inflammation, proliferation and remodelling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration and treatment of dermal diseases, including the stem cell-derived, plant-derived and engineered EVs.

scholarly journals Extracellular Vesicles in Skin Wound Healing

2021 ◽  
Vol 14 (8) ◽  
pp. 811
Author(s):  
Deimantė Narauskaitė ◽  
Gabrielė Vydmantaitė ◽  
Justina Rusteikaitė ◽  
Revathi Sampath ◽  
Akvilė Rudaitytė ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Current Knowledge ◽  
Skin Wound ◽  
Surface Proteins ◽  
Therapeutic Interventions ◽  
Skin Wound Healing ◽  
Skin Repair ◽  
Healing Wound ◽  
Healing Phase

Each year, millions of individuals suffer from a non-healing wound, abnormal scarring, or injuries accompanied by an infection. For these cases, scientists are searching for new therapeutic interventions, from which one of the most promising is the use of extracellular vesicles (EVs). Naturally, EV-based signaling takes part in all four wound healing phases: hemostasis, inflammation, proliferation, and remodeling. Such an extensive involvement of EVs suggests exploiting their action to modulate the impaired healing phase. Furthermore, next to their natural wound healing capacity, EVs can be engineered for better defined pharmaceutical purposes, such as carrying specific cargo or targeting specific destinations by labelling them with certain surface proteins. This review aims to promote scientific awareness in basic and translational research of EVs by summarizing the current knowledge about their natural role in each stage of skin repair and the most recent findings in application areas, such as wound healing, skin regeneration, and treatment of dermal diseases, including the stem cell-derived, plant-derived, and engineered EVs.

scholarly journals Extracellular Vesicles from Adipose-Derived Mesenchymal Stem/Stromal Cells Accelerate Migration and Activate AKT Pathway in Human Keratinocytes and Fibroblasts Independently of miR-205 Activity

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Andrea da Fonseca Ferreira ◽  
Pricila da Silva Cunha ◽  
Virgínia Mendes Carregal ◽  
Priscila de Cássia da Silva ◽  
Marcelo Coutinho de Miranda ◽  
...  
Keyword(s):  
Wound Healing ◽  
Extracellular Vesicles ◽  
Stromal Cells ◽  
Average Diameter ◽  
Skin Wound ◽  
Akt Pathway ◽  
Skin Wound Healing ◽  
Akt Phosphorylation ◽  
Akt Activation

Mesenchymal stem/stromal cells (MSCs) are promising tools in cell therapy. They secrete extracellular vesicles (EVs) that carry different classes of molecules that can promote skin repair, but the mechanisms are poorly understood. Skin wound healing is a complex process that requires the activity of several signaling pathways and cell types, including keratinocytes and fibroblasts. In this study, we explored whether adipose tissue MSC-derived EVs could accelerate migration and proliferation of keratinocytes and fibroblasts, activate the AKT pathway, and promote wound healing in vivo. Furthermore, we evaluated if EV effects are miR-205 dependent. We found that MSC EVs had an average diameter of 135 nm. Keratinocytes and fibroblasts exposed to EVs exhibited higher levels of proliferation, migration, and AKT activation. Topical administration of EVs accelerated skin wound closure. Knockdown of miR-205 decreased AKT phosphorylation in fibroblasts and keratinocytes, whereas migration was decreased only in keratinocytes. Moreover, knockdown of miR-205 failed to inhibit AKT phosphorylation in fibroblasts and keratinocytes exposed to EVs. About the mechanism of EV effects, we found that incubation with EVs prevented inhibition of AKT activation by miR-205 knockdown, suggesting that EVs activate AKT independently of miR-205. In conclusion, we demonstrated that EVs are a promising tool for wound healing.

scholarly journals Estrogen promotes cutaneous wound healing via estrogen receptor β independent of its antiinflammatory activities

2010 ◽  
Vol 207 (9) ◽  
pp. 1825-1833 ◽  
Author(s):  
Laura Campbell ◽  
Elaine Emmerson ◽  
Faith Davies ◽  
Stephen C. Gilliver ◽  
Andre Krust ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antiinflammatory Activity ◽  
Hormone Replacement ◽  
Skin Wound ◽  
The Body ◽  
Estrogen Replacement ◽  
Skin Wound Healing ◽  
Skin Repair ◽  
Increased Risk

Post-menopausal women have an increased risk of developing a number of degenerative pathological conditions, linked by the common theme of excessive inflammation. Systemic estrogen replacement (in the form of hormone replacement therapy) is able to accelerate healing of acute cutaneous wounds in elderly females, linked to its potent antiinflammatory activity. However, in contrast to many other age-associated pathologies, the detailed mechanisms through which estrogen modulates skin repair, particularly the cell type–specific role of the two estrogen receptors, ERα and ERβ, has yet to be determined. Here, we use pharmacological activation and genetic deletion to investigate the role of both ERα and ERβ in cutaneous tissue repair. Unexpectedly, we report that exogenous estrogen replacement to ovariectomised mice in the absence of ERβ actually delayed wound healing. Moreover, healing in epidermal-specific ERβ null mice (K14-cre/ERβL2/L2) largely resembled that in global ERβ null mice. Thus, the beneficial effects of estrogen on skin wound healing are mediated by epidermal ERβ, in marked contrast to most other tissues in the body where ERα is predominant. Surprisingly, agonists to both ERα and ERβ are potently antiinflammatory during skin repair, indicating clear uncoupling of inflammation and overall efficiency of repair. Thus, estrogen-mediated antiinflammatory activity is not the principal factor in accelerated wound healing.

scholarly journals Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin ofCopaifera langsdorffiiDesf. Kuntze in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Lucas Fernando Sérgio Gushiken ◽  
Carlos Alberto Hussni ◽  
Jairo Kenupp Bastos ◽  
Ariane Leite Rozza ◽  
Fernando Pereira Beserra ◽  
...  
Keyword(s):  
Wound Healing ◽  
Immunohistochemical Analysis ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Standard Drug ◽  
Hydroalcoholic Extract ◽  
Skin Repair ◽  
Complex Process ◽  
Copaifera Langsdorffii ◽  
New Treatments

The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) fromCopaifera langsdorffiiand hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, maleWistarrats were divided into groups (n=8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity ofCopaifera langsdorffiicreams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms.

scholarly journals Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing

2021 ◽  
Author(s):  
Hua Qu ◽  
Tian Miao ◽  
Yuren Wang ◽  
Liang Tan ◽  
Bangliang Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Epidermal Keratinocytes ◽  
Skin Wound Healing ◽  
Genetic Ablation ◽  
Skin Repair ◽  
Diabetic Wound Healing ◽  
Animal Models Of Diabetes ◽  
Laminin 332

Cutaneous wound healing is a fundamental biological and coordinated process, and failure to maintain this process contributes to the dysfunction of tissue homeostasis, increasing the global burden of diabetic foot ulcerations. However, the factors that mediate this process are not fully understood. Here, we identify dedicator of cytokinesis 5 (Dock5) a pivotal role in keratinocyte functions contributing to the process of skin wound healing. Specifically, Dock5 is highly upregulated during the proliferative phase of wound repair and is predominantly expressed in epidermal keratinocytes. It regulates keratinocyte adhesion, migration and proliferation, and influences the functions of extracellular matrix (ECM) deposition by facilitating the ubiquitination of transcription factor ZEB1 to activate laminin-332/integrin signaling. Genetic ablation of Dock5 in mice leads to attenuated re-epithelialization and granulation tissue formation, while Dock5 overexpression-improved skin repair can be abrogated by LAMA3 knockdown. Importantly, Dock5 expression in the skin edge is reduced in patients and animal models of diabetes, further suggesting a direct correlation between its abundance and healing capability. The rescue of Dock5 expression in diabetic mice causes a significant improvement in re-epithelialization, collagen deposition, ECM production and granulation. Our study provides a potential therapeutic target for wound healing impairment during diabetes.

Bone marrow-derived mesenchymal stem cells and extracellular vesicles enriched collagen chitosan scaffold in skin wound healing (a rat model)

2020 ◽  
pp. 088532822096392
Author(s):  
Salma Abolgheit ◽  
Sally Abdelkader ◽  
Moustafa Aboushelib ◽  
Enas Omar ◽  
Radwa Mehanna
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Tensile Strength ◽  
Mesenchymal Stem Cells ◽  
Extracellular Vesicles ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Skin Wound Healing ◽  
Chitosan Scaffolds

Background Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold’s effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing. Methods A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8). Results The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group. Conclusion The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.

scholarly journals Gene Expression Profiling of the Extracellular Matrix Signature in Macrophages of Different Activation Status: Relevance for Skin Wound Healing

2019 ◽  
Vol 20 (20) ◽  
pp. 5086 ◽  
Author(s):  
Julia Etich ◽  
Manuel Koch ◽  
Raimund Wagener ◽  
Frank Zaucke ◽  
Mario Fabri ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Transcriptome Profiling ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Skin Repair

The extracellular matrix (ECM) provides structural support for tissue architecture and is a major effector of cell behavior during skin repair and inflammation. Macrophages are involved in all stages of skin repair but only limited knowledge exists about macrophage-specific expression and regulation of ECM components. In this study, we used transcriptome profiling and bioinformatic analysis to define the unique expression of ECM-associated genes in cultured macrophages. Characterization of the matrisome revealed that most genes were constitutively expressed and that several genes were uniquely regulated upon interferon gamma (IFNγ) and dexamethasone stimulation. Among those core matrisome and matrisome-associated components transforming growth factor beta (TGFβ)-induced, matrix metalloproteinase 9 (MMP9), elastin microfibril interfacer (EMILIN)-1, netrin-1 and gliomedin were also present within the wound bed at time points that are characterized by profound macrophage infiltration. Hence, macrophages are a source of ECM components in vitro as well as during skin wound healing, and identification of these matrisome components is a first step to understand the role and therapeutic value of ECM components in macrophages and during wound healing.

Sign in / Sign up

Export Citation Format

Share Document