scholarly journals Fruit Morphology, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Salacca zalacca (Gaertner) Voss by Applications of Goat Manures and Bacillus velezensis B-27

2021 ◽  
Vol 36 (2) ◽  
pp. 270
Author(s):  
Haviah Hafidhotul Ilmiah ◽  
Endang Sulistyaningsih ◽  
Tri Joko
Keyword(s):  
Antioxidant Activity ◽  
Block Design ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Fruit Morphology ◽  
Bacillus Velezensis ◽  
Fruit Length ◽  
Phenolic Contents ◽  
Goat Manure ◽  
Phenolic And Flavonoid

<p>Snake fruit (<em>Salacca zalacca</em> (Gaertner) Voss) is one of indigenous fruits from Southeast Asia that has been consumed for its antioxidant contents. Improving the fruit quality might increase its benefits for human health. This study aims to analyze fruit morphology, antioxidant activity, total phenolic and flavonoid contents of the fruit applied with goat manures and <em>Bacillus velezensis </em>B-27. The research used two-factor Randomized Complete Block Design (RCBD) with three blocks as replications. The first factor is the level of goat manure application i.e. 0 kg plant<sup>-1</sup>, 5 kg plant<sup>-1</sup> and 10 kg plant<sup>-1</sup>, while the second factor is the bacteria application i.e. applied with <em>Bacillus velezensis </em>B-27 and without<em> Bacillus velezensis </em>B-27. The research was conducted in June until December 2019 at Turi, Sleman, Yogyakarta, Indonesia. Fruit length, diameter and shape were measured as morphology parameters. Antioxidant activities were measured using 2,2-Diphenyl-1-picrylhydrazyl, whereas flavonoid and phenolic contents were determined by the aluminium-chloride colorimetric and Folin-Ciocalteu methods, respectively. Means of each parameter were analyzed using ANOVA, continued with the Tukey’s HSD test at a 5% significance level. The result showed that the application of 10 kg goat manure per plant with bacteria increased the fruit length (76.78 mm) and the diameter (62.72 mm). Addition of 10 kg goat manure per-plant combined with <em>Bacillus velezensis </em>B-27 gave the highest antioxidant (IC50 of 37.83 µg.mL<sup>-1</sup>), flavonoid (5.35 mgGAE.100 g<sup>-1</sup>) and total phenolic contents (44 mgQE.100 g<sup>-1</sup>).</p>

scholarly journals Determination of total phenolic and flavonoid contents of jackfruit peel and in vitro antiradical test

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 84-90
Author(s):  
Yamin ◽  
Ruslin ◽  
Mistriyani ◽  
Sabarudin ◽  
S. Ihsan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Colorimetric Method ◽  
Ethyl Acetate Fraction ◽  
Premature Aging ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Free radical is any molecular species that have unpaired free electrons in their outer orbital shell that make radicals highly reactive, resulting in pathogenesis conditions such as cellular injury, premature aging, cancer, hepatic disorders, neurodegenerative diseases, cardiovascular disease, and kidney disease. One source of natural antioxidant is jackfruit. The purpose of this research was to determine the phenolic and flavonoid contents in the extracts and fractions of jackfruit peel and their potential as antioxidants. Jackfruit peel powder was extracted from maceration. The total phenolic content was determined by the Folin-Ciocalteu method. Meanwhile, flavonoid content was determined using the aluminium chloride complex colorimetric method. Measurements of antioxidant activity were conducted using the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The ethyl acetate fraction had high phenolic and flavonoid contents, which were 49.667±1.508 g GAE/100 g of sample and 70.199±0.374 g of quercertin equivalent/100 g. The ethyl acetate fraction had the strongest antioxidant activity with IC50 value of 4.539±0.201 µg/ mL and correlation value (R2 ) of 0.5881 for phenols and R2 of 0.7241 for flavonoids. Ethyl acetate fraction of jackfruit peel is very potential to be developed as a natural antioxidant and functional food.

Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

2019 ◽  
Vol 5 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Vinod Kumar Gautam ◽  
Mannu Datta ◽  
Ashish Baldi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Seasonal Variations ◽  
Summer Season ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Spring Season ◽  
Phenolic Contents ◽  
Aerial Parts ◽  
Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

Influence of total phenolic content and total flavonoid content on the DPPH radical scavenging activity of Costus speciosus (Koen Ex. Retz.) Sm.

2015 ◽  
Vol 4 (5) ◽  
pp. 261-266
Author(s):  
M. Borkataky ◽  
B. B. Kakoti ◽  
L. R. Saikia
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Costus Speciosus ◽  
Phenolic And Flavonoid

  The focus of the present study was to determine the association of the anti-oxidant activity of Costus speciosus (Koen Ex. Retz.) Sm. with the total phe-nolic content and the total flavonoid content. The antioxidant activity of the extracts was quantified by diphenylpicrylhydrazyl (DPPH) assay. The total phenolic content was determined by the Folin-Ciocalteau method and the total flavonoid content was determined by the aluminium chloride method. Among the four extracts of the plant, the ethanol extract exhibited the high-est antioxidant activity and also the highest total phenolic and flavonoid con-tents. A strong correlation was observed between the antioxidant activity and the total phenolic and flavonoid contents. A statistical model was de-rived to explain this dependence and a non-linear association was observed between the antioxidant activity and the total flavonoid content.

scholarly journals Antioxidant activity of Clematis brachiata Thunb. leaf

2018 ◽  
Vol 53 (3) ◽  
pp. 185-190 ◽  
Author(s):  
M Mostafa ◽  
S Ahmed ◽  
AJ Afolayan
Keyword(s):  
Antioxidant Activity ◽  
Dry Matter ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Good Source ◽  
Diammonium Salt ◽  
Phenolic And Flavonoid

The antioxidant activity, total phenolic and flavonoid contents of different extracts of the Clematis brachiata Thunb leaves were determined. The antioxidant activity was evaluated using spectroscopic methods against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2′-azinobis [3-ethylbenzothiazoline-6-sulphonic acid] diammonium salt radical cation (ABTS). Folin Ciocalteu method was used to determine the total phenolics and Aluminium Chloride Colorimetric method was used to determine the flavonoids contents in these extracts. The results showed that the methanol extract of the leaf exhibited the highest antioxidant activity with the value of 180.45 ±2.4 μg mL-1 in DPPH and 60 ±0.80 μg mL-1 in ABTS assay among the extracts. The methanol extract contains more phenolic compounds (178±2.20 mg/g as galic acid equivalent per g dry matter) and the acetone extract contains more flavonoids (135.11±1.20 mg/g as quercitin equivalent per g dry matter) among the extracts. This study provides the evidence that the leaves of the Clematis brachiata Thunb could be a good source of natural antioxidant.Bangladesh J. Sci. Ind. Res.53(3), 185-192, 2018

scholarly journals Antioxidant and antiproliferative activities of the n-butanol extract of Centaurea maroccana Ball aerial parts

2021 ◽  
Vol 34 (1) ◽  
pp. 5-11
Author(s):  
Imen Aissous ◽  
Mouad Benrebai ◽  
Ercan Cacan ◽  
Berkel Caglar ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Proliferative Activity ◽  
Homo Sapiens ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Butanolic Extract

Abstract The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the quantitative estimation of total flavonoids and phenols revealed the richness of the extract in these compounds. Antioxidant activity was evaluated using standard lab colorimetric methods, while the anti-proliferative activity was evaluated using sulforhodamine B (SRB) assay against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) cell lines. The n-butanolic extract of Centaurea maroccana showed a strong antioxidant activity through DPPH, ABTS•+, DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore, the anti-proliferative activity against C6 and HT29 of Centaurea maroccana exhibited a high effect by the decrease in viability of both cancer cell lines. Our results suggest a possible use of Centaurea maroccana as a source of natural antioxidant and chemo-preventive agents against cancer.

scholarly journals Changes of Phenolic Contents and Antioxidant Activity in Soybean Seeds Harvested from Phakopsora pachyrhizi Infected Crops

2018 ◽  
Vol 10 (2) ◽  
pp. 369-378
Author(s):  
Eriyanto Yusnawan ◽  
Novita Nugrahaeni ◽  
Joko Susilo Utomo
Keyword(s):  
Antioxidant Activity ◽  
Seed Weight ◽  
Block Design ◽  
Total Flavonoid ◽  
Soybean Rust ◽  
Total Phenolic ◽  
Soybean Seeds ◽  
Phakopsora Pachyrhizi ◽  
Rust Infection ◽  
Phenolic Contents

Asian soybean rust caused by Phakopsora pachyrhizi is one of the most destructive foliar diseases on soybean. Severe infection of this disease causes early defoliation and reduces the yield. To determine the response of soybean genotypes to this disease and the changes of metabolites in seeds, a greenhouse study was conducted using eight Indonesian soybean cultivars, i.e. Malabar, Wilis, Ringgit, Pangrango, Argomulyo, Grobogan, Dena 1, and Dena 2. The experiment was arranged in a randomized completely block design and repeated three times. The soybean crops were inoculated with the pathogen and another set was not inoculated. Infection of P. pachyrhizi reduced fresh biomass, seed weight per plant, and weight of 100 seeds. However, total flavonoid contents in seeds increased from 12 to 50% in all infected genotypes. The increase of daidzein from 27 to 67% in seeds was observed, except for Malabar and Argomulyo . The increase of genistein was genotypic dependence. The increase of total phenolic contents as well as antioxidant activity was also depending on the genotypes. P. pachyrhizi could be one of the biotic elicitors to increase total flavonoid contents in soybean seeds. Dena 1 less suffered from the rust infection as represented by the least pustule number, less reduction in seed weight and weight of 100 seeds. Secondary metabolites particularly phenolics and isoflavones in seeds of this cultivar increased significantly after the rust infection. This cultivar could be considered as an alternative tolerant genotype where cultivated area is favorable for soybean rust infection.

scholarly journals Evaluation of Total Flavonoid, Total Phenolic Contents, and Antioxidant Activity of Strychnobiflavone

2020 ◽  
Vol 20 (3) ◽  
pp. 716 ◽  
Author(s):  
Sana Ullah ◽  
Chang-Gu Hyun
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Antioxidant Agent ◽  
Increasing Demand ◽  
Phenolic And Flavonoid

This work evaluates the antioxidant activity of strychnobiflavone due to the increasing demand of the antioxidant agents day by day. Various in vitro antioxidants assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used to investigate the antioxidant activity of strychnobiflavone. The results of both DPPH and ABTS show that strychnobiflavone increase the scavenging activity in a concentration-dependent manner due to the phenolic and flavonoid contents. This study revealed that strychnobiflavone is one of the promising and an effective compound for the antioxidant agent.

scholarly journals Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots

2021 ◽  
Vol 18 (7) ◽  
pp. 1499-1506
Author(s):  
Nacer Amraoui ◽  
Nozha Mayouf ◽  
Noureddine Charef ◽  
Abderrahmane Baghiani ◽  
Lekhmici Arrar
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Dry Weight ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Paw Edema ◽  
Adjuvant Induced Arthritis ◽  
Anti Inflammatory ◽  
Phenolic And Flavonoid

Purpose: To evaluate the antioxidant, anti-inflammatory and anti‐arthritic effects of the methanol extract of Tamus communis roots. Methods: The total phenolic and flavonoid contents were determined using Folin–Ciocalteu’s reagent and aluminium chloride assays, respectively while the antioxidant capacity was determined using DPPH and β-carotene/linoleate tests. For anti-inflammatory activity, carrageenan-induced paw edema in rat was used. Formaldehyde-induced paw edema and adjuvant-induced arthritis in rats were used to evaluate the anti-arthritic effect of the extract. Results: The total phenolic and flavonoid contents of T. communis showed 55.2 ± 0.036 mg GAE/g dry weight and 1.191 ± 0.002 mg QEq/g dry weight, respectively while the antioxidant activity (DPPH) showed a half-maximal inhibitory concentration (IC50 of 0.128 ± 0.011 mg/mL. Using βcarotene/linoleate assay, the extract showed 88.13 ± 4.03 % inhibition. In carrageenan-induced paw edema in rat, all doses of T. communis did not show inhibition of edema. In contrast, formaldehyde induced paw edema decreased at the dose of 150 mg/kg days 2 and 4. However, doses of 300 and 600 mg/kg did not show any activity. In adjuvant-induced arthritis, all used doses caused no reduction in arthritic scores and in paw thickness except a significant decrease with the dose 150 mg/kg at the last day. Conclusion: T. communis extract exhibits high antioxidant activity related to the phenolic compound contents. However, the results of the anti-inflammatory and anti‐arthritic studies did not support its use in folk medicine in the treatment of rheumatic ailment except on low doses

scholarly journals Antioxidant activity and total phenolic and flavonoid contents of Chrysopogon aucheri (Boiss.) Stapf

2017 ◽  
Vol 63 (2) ◽  
pp. 42-47
Author(s):  
Maryam Sadoughinia ◽  
Jinous Asgarpanah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Aluminium Chloride ◽  
Biological Research ◽  
Total Phenolic ◽  
Total Extract ◽  
Phenolic And Flavonoid

Summary Introduction: Chrysopogon aucheri is a species native to the South of Iran. It is used for the treatment of some oxidative-based disorders. Objective: Due to lack of biological research on C. aucheri, we were prompted to investigate the antioxidant activities of leaf total extract and different sub-fractions for the first time. The phenolic and flavonoid contents were also determined in the leaves as the interaction between these components and the antioxidant activity. Methods: The antioxidant effects of total extract and sub-fractions were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic and flavonoid contents were also determined by Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. Results: Total extract of C. aucheri leaves was found to possess desirable antioxidant activity in vitro in comparison with standard antioxidant BHT (butylated hydroxytoluene). The highest phenolic and flavonoid contents were observed in the methanol sub-fraction. Results showed weak and moderate correlations with phenolics and flavonoids, respectively, and therefore other metabolites of C. aucheri leaves might be responsible for antioxidant activities. Conclusion: The results suggested that C. aucheri leaves possess desirable antioxidant activity when compared with BHT and support the ethnomedicinal claims of the use of the leaves in the management of some oxidative-based diseases.

Molecular and phytochemical characterization of F1 Streptocarpus hybrids and antioxidant potential of their flower extracts

2020 ◽  
Vol 48 (3) ◽  
pp. 1341-1356
Author(s):  
Monica HȂRŢA ◽  
Doru PAMFIL ◽  
Orsolya BORSAI ◽  
Rodica POP ◽  
Doina CLAPA ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Genetic Relationships ◽  
Flower Colour ◽  
Chemical Compounds ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Polyphenolic Content ◽  
Pharmaceutical Industries ◽  
Phenolic And Flavonoid

Streptocarpus x hybridus is a very popular houseplant with colourful flowers arousing thus the interest of plant breeders. Its potential value is even higher since different chemical compounds could be identified and used for food and pharmaceutical industries. Therefore, the objectives of this study were: to determine genetic relationships between twelve F1 Streptocarpus hybrids and their genitors; to determine the total phenolic and flavonoid content; to evaluate the antioxidant activity of the analysed individuals. Although 12 out of the 24 RAPD primers detected polymorphism among the individuals, the UPGMA dendrogram did not show a very clear grouping pattern for flower colour. In contrast, the phytochemical dendrogram shed more light on the dissimilarities among Streptocarpus flowers in terms of their polyphenolic content. The total phenolic and flavonoid content of the flower extracts determined by the Folin-Ciocalteau and aluminium chloride colorimetric methods ranged from 680.02 to 2360.57 mg of GAE/100 g FW and from 12.52 to 78.36 mg QE/100 g FW. The radical scavenging activity against ABTS ranged from 27.17 to 130.35 µM Trolox/g FW. Due to its ornamental value, H12 was screened by HPLC-PDA-ESI-MS to identify and quantify the chemical compounds involved in flower colour. Among the six compounds that were identified and quantified (i.e. cyanidin-3-O-galactoside, cyanidin-3-O-xyloside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, peonidin-3-O-glucoside and apigenin-6-C-glucoside), isovitexin reached the highest concentration 4183 µg rutin/100 g FW. These preliminary results, showing high total polyphenolic content and ABTS antioxidant activity, suggest that Streptocarpus flowers could be a valuable source of antioxidants.

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