scholarly journals In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations

2021 ◽  
Vol 1 (1) ◽  
pp. 17
Author(s):  
Samanhudi Samanhudi ◽  
Ahmad Yunus ◽  
Rahmanto Rahmanto
Keyword(s):  
Tissue Culture ◽  
Growth Parameters ◽  
Growth Potential ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Leaf Emergence ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Shoot Emergence

<p><em>Tribulus terrestris</em> is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of <em>T. terrestris</em> in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time.</p><p> </p><p align="left">Keywords: explant; plant growth; tissue culture.</p>

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

2021 ◽  
Vol 905 (1) ◽  
pp. 012036
Author(s):  
Nandariyah ◽  
L S Mahmudah ◽  
R B Arniputri ◽  
A T Sakya
Keyword(s):  
Tissue Culture ◽  
Coconut Water ◽  
Emergence Time ◽  
Shoot Height ◽  
Culture Techniques ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

scholarly journals Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fast Time ◽  
Endogenous Auxin ◽  
Seed Technology ◽  
Randomized Design ◽  
Process Plants ◽  
Number Of Leaves ◽  
Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

scholarly journals تأثير تراكيز مختلفة من السكر في بيئة زراعة الأنسجه على نمو نبات الورد صنف ساره (Sara)

2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله
Keyword(s):  
Growth Parameters ◽  
Growth Media ◽  
Fresh Weight ◽  
Negative Results ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Branches

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.

scholarly journals Pertumbuhan Tanaman Pisang Kepok (Musa paradisiaca L.) pada Berbagai Konsentrasi BAP Secara In Vitro

2014 ◽  
Vol 4 (1) ◽  
pp. 220-224
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Mahdiannoor Mahdiannoor ◽  
Lisa Lisa
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
In Vitro Culture ◽  
Root Formation ◽  
Shoot Formation ◽  
Experimental Unit ◽  
Culture Techniques ◽  
Randomized Design ◽  
Number Of Leaves

       Bananas are one of the horticultural commodities is very popular in the world because it was delicious, the price is relatively cheap and have high nutritional value. Production of banana plants in Hulu Sungai Utara still relatively low because only bananas cultivated on a small and not available the procurement of seeds. To overcome these obstacles do necessary aside propagated using tissue culture techniques. ZPT which are often used to stimulate the formation of shoots in vitro culture banana is cytokinin as BAP. Cytokines in vitro culture has two important roles that stimulate cell division and the formation and propagation of axillary buds and adventitious buds, but the optimum levels of cytokines that can inhibit the growth and root formation. The purpose of this study is to get (i) the effect of various concentrations of BAP on plant growth kepok banana in vitro, (ii) the best BAP concentration for plant growth kepok banana in vitro. This research was conducted in tissue culture laboratory STIPER Amuntai in April 2013 to August 2013 using a completely randomized design (CRD) single factor with 5 treatments and 4 replications, so there are 20 experimental units. Each experimental unit consisted of 2 explants as a sample. Factors that are tested various concentrations of BAP are b1 : 2 mg / l, b2 : 3 mg / l, b3 : 4 mg / l, b4 : 5 mg / l, b5 : 6 mg / l. The results showed that percentage grows, the percentage of shoot formation, root formation percentage, number of shoots and number of leaves had no effect on treatment.

scholarly journals THE GROWTH OF JACKFRUIT (ARTOCARPUS HETEROPHYLLUS L.) SHOOTS ON VARIOUS CONCENTRATION BENZYLAMINO PURINE (BAP) IN VITRO

2019 ◽  
Vol 5 (2) ◽  
pp. 99
Author(s):  
Frisca Nanda Bulo ◽  
Enny Adelina ◽  
Ramal Yusuf ◽  
Hawalina Kasim
Keyword(s):  
Tissue Culture ◽  
Plant Biotechnology ◽  
Culture Technique ◽  
Artocarpus Heterophyllus ◽  
Conventional Breeding ◽  
Resistant Cultivars ◽  
Number Of Leaves ◽  
Central Sulawesi ◽  
Shoot Emergence

The development of Central Sulawesi superior commodity,which is Tulo-5, the drough-resistant cultivars, through tissue culture technique can be used as an alternative, in addition to conventional breeding. This study aims to determine the growth of jackfruit shoots in  various concentrations of benzylamino purine in vitro. The research has been conducted at the Laboratory of Plant Biotechnology, Agriculture Faculty of Tadulako University, Palu.  The research began on December 2017 and ended up on February 2018. This research used Completely Randomised Design of one-factor treatment which is BAP concentrations consist of 4 stage which is1,5 ppm BAP, 2,0 ppm BAP, 2,5 ppm BAP, 3,0 ppm BAP. The result showed that 6 weeks after planting, BAP concentration had significant effect on shoots time to emerge and shoots number but no significant effect on leaf number.  The result of Tukey’s HSD test showed that concentration2,5 ppm gave the fastest shoot emergence with an average of 2,875 days after planting, in concentration 2,0 ppm gave the highest number of shoots with an average of 2.125 shoots per explant, and for the highest number of leaves found in the concentration of 2,0 ppm with the average number of leaves is 1 strand per explant. 

scholarly journals Micropropagation of Vanda tricolor Lindl. var. suavis with various concentrations of organic growth supplements

2021 ◽  
Vol 886 (1) ◽  
pp. 012004
Author(s):  
M Tuwo ◽  
A I Latunra ◽  
E T Ana
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Good Alternative ◽  
Control Treatment ◽  
Tissue Culture Media ◽  
Organic Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves

Abstract Plant propagation through in vitro culture is increasingly being used to produce hybrid orchids. Plant tissue culture provides a good alternative to produce plants in large numbers in a short time. The provision of organic growth supplements in tissue culture media plays an important role as a substitute for growth regulators in stimulating the growth of explants. In this study, young coconut water, banana extract (cv. ambon), and tomato extract were used to stimulate the growth of the Vanda tricolor Lindl. Var suavis protocorm. Data were analyzed using a completely randomized design (CRD) with five replications using the Kruskal-Wallis test and continued with the Mann-Whitney test if there was a significant difference between each treatment and its concentration. Parameters observed were the percentage of the number of shoots and the number of leaves. The results showed that there was a significant difference between the treatments given to the number of leaves. Mann-Whitney further test results on the number of leaves showed a significant difference in the banana extract treatment and the control treatment.

scholarly journals Addition of Benzyl Amino Purine (BAP) to Induction of Drendan Seeds Buds (Lansium domesticum var. aqueum (Jack) Miq.) by In Vitro

2021 ◽  
Vol 8 (2) ◽  
pp. 61
Author(s):  
Mayta Novaliza Isda ◽  
Febby Ika Desyana
Keyword(s):  
Shoot Formation ◽  
Shoot Length ◽  
Shoot Induction ◽  
Emergence Time ◽  
Randomized Design ◽  
High Antioxidant Activity ◽  
Short Time ◽  
Lansium Domesticum ◽  
Shoot Emergence

Drendan (Lansium domesticum var. aqueum (Jack) Miq. is one of Indonesia's native tropical fruits. Drendan has high antioxidant activity and contains high nutrients, but has started to rarely be found in the field because its management and cultivation is rarely done due to its sour taste in vitro culture is a method or technique of plant propagation which can be an alternative to obtaining drendan seedlings in large quantities and in a short time vegetatively. The purpose of this study was to determine the effect of BAP on shoot induction from drendan seed explants and determine the best concentration of BAP on shoot growth of drendan seed explants in vitro. This study used a completely randomized design with BAP concentrations of 0, 1, 3, 5 and 7 mg / L BAP. The result of this research is the addition of BAP has been able to increase the percentage of live explants and the percentage of shoot formation. Giving BAP did not significantly affect the number of shoots and had a significant effect on shoot emergence time and shoot length. The addition of BAP resulted in slower shoot emergence time. Giving BAP was not able to stimulate shoot induction, but the addition of BAP could increase shoot length growth at a concentration of 5 mg / L BAP of 0.52 cm.

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

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