scholarly journals Assessment of the Use of Gentian Violet and Malachite Green as Selective Agents in the Isolation of Brucella

2021 ◽  
pp. 60-65
Author(s):  
Yu. S. Kovtun ◽  
A. A. Kurilova ◽  
L. S. Katunina ◽  
E. I. Vasilenko
Keyword(s):  
Malachite Green ◽  
Brucella Abortus ◽  
Nutrient Medium ◽  
Gentian Violet ◽  
Shigella Flexneri ◽  
Medium Effect ◽  
Proteus Vulgaris ◽  
Nutrient Media ◽  
Selective Agents ◽  
The Media

The aim of the work is to evaluate ability of gentian violet and malachite green to inhibit 2 Brucella test strains and 8 strains of unwanted organisms and the influence of autoclaving on their activity. The study also aims to evaluate variability of inhibition in different batches of colorants and necessity in adjustment of their concentration.Materials and methods. The study included 3 imported lots of gentian violet and malachite green. Inhibitors were put into nutrient medium containing pancreatic hydrolysates of gelatin and fsh meal, yeast extract, sodium chloride, glucose, sodium pyrosulfate, and agar. The same medium without colorants was used as a control medium. Effect of the colorants was studied with the help of test strains of Brucella abortus 19 ВА, B. melitensis Rev I, Enterococcus faecium VKM B-602, Escherichia coli ATCC 25922, Proteus vulgaris HX 19 222, Pseudomonas aeruginosa 22/99, Serracia plymuthica 1, Shigella flexneri 1a 8516, Staphylococcus aureus ATCC 25923, Yersinia enterocolitica C-187.Results and discussion. We have determined biological parameters of nutrient media containing gentian violet (5,0; 2,5 1,25 mg/L) and malachite green (2.0; 1.0; 0.5 mg/L) when cultivating brucella. Gentian violet is able to inhibit Brucella greatly at concentration of 5 mg/L. S. aureus ATCC 25923 did not show any growth on the media containing malachite green or gential violet (5.0 and 2.5 mg/L). Malachite green inhibited the growth of enterococcus more actively than gentian violet. However, it inhibited swarming growth of Proteus less successfully than gentian violet. Other unwanted organisms were not sensitive to such concentrations of colorants at all. We have demonstrated the necessity to combine colorants with other selective agents, as well as advisability to put gentian violet into sterile media, while malachite green – into the media before its autoclaving. Adjustments to the concentration of gentian violet should be made when producing nutrient medium, as variations in inhibiting activity in different batches of this colorant were revealed.

scholarly journals FEATURES OF MASS-SPECTROMETRIC PROTEIN PROFILES OF STRAINS OF BRUCELLOSIS CAUSATIVE AGENT DURING PREPARATION OF CULTURE ON VARIOUS NUTRIENT MEDIA

2016 ◽  
pp. 29-34 ◽  
Author(s):  
D. V. Ulshina ◽  
D. A. Kovalev ◽  
A. M. Zhirov ◽  
N. V. Zharinova ◽  
A. A. Khudoleev ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Nutrient Medium ◽  
Brucella Melitensis ◽  
Mass Spectrometric ◽  
Protein Profiling ◽  
Spectrometric Analysis ◽  
Protein Profiles ◽  
Nutrient Media ◽  
Linear Mode ◽  
Maldi Tof

Aim. Carry out comparative analysis using time-of-flight mass-spectrometry with matrix laser desorption/ionization (MALDI-TOF MS) of protein profiles of brucellosis causative agents (Brucella melitensis Rev-1 and Brucella abortus 19BA), cultivated in various nutrient media: Albimi agar, brucellagar and erythrit-agar. Materials and methods. Vaccine strains: Brucella melitensis Rev-1 and Brucella abortus 19BA. Protein profiling in linear mode on Microflex «Bruker Daltonics» MALDI-TOF mass-spectrometer. Results. A number of characteristic features of brucella mass-spectra was detected: in particular, preservation of the total qualitative composition of protein profiles of cultures and significant differences in the intensity of separate peaks depending on the nutrient medium used. Conclusion. Based on the analysis of the data obtained, use of Albimi agar as the nutrient medium for preparation of brucella culture samples for mass-spectrometric analysis was shown to be optimal.

scholarly journals THE PRODUCTION OF SELECTIVE ADDITIVES TO GROWING MEDIUM DRIGALSKI LACTOSE AGAR

2019 ◽  
pp. 95-102
Author(s):  
Владимир Ермаков ◽  
Vlyadimir Ermakov ◽  
Оксана Датченко ◽  
Oksana Datchenko ◽  
Юлия Курлыкова ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
First Generation ◽  
Animal Species ◽  
Agar Medium ◽  
Nutrient Media ◽  
Gram Negative ◽  
Selective Media ◽  
Growing Medium ◽  
Agar Media ◽  
Growth And Reproduction

The purpose of the study is to improve the selective supplement for selective media with the purpose to produce enterobacteria. Tasks of the study are to identify the sensitivity of strains obtained of enterobacteria in regard to an-tibiotics; develop a new selective supplement with antibiotics to the nutrient medium Drigalski Lactose Agar. Media should have a content that in the best way possible ensures the growth and reproduction of microorganisms of cer-tain species or family. Intensive biotechnology development and Microbiology allows today to develop new nutrient media and modify the already existing content of media. The object of the study was a new selective additive with antibiotics to the nutrient medium Drigalski Lactose Agar. 253 isolates of bacteria produced from the intestinal mi-crobiotope of different animal species have been the Material for research. The study was conducted in the period from 2010 to 2017. Carbenicillin 30±2.3 from the group of carboxypenicillins and piperacillin 37±2.5 from the group of ureidopenicillins, kanamycin 24±1.5, amikacin 26±1.7 and gentamicin 25±0,8, cefepime 38±3.2 from the group of IV generation cephalosporins, tetracycline 28±1.6, doxycycline 34±2.3 and chloramphenicol 31±2.5, nalidixic acid 37±2.8, trimethoprim 35±3,4 demonstrated the greatest antimicrobial activity against all cultures of enterobac-teria that has been achieved. The high resistance of enterobacteria was shown to benzylpenicillin from the group of natural penicillins, to streptomycin, cephalotine from the group of cephalosporins of the first generation, to polymyx-in B, to ofloxacin (tarivid) and metronidazole. Antibacterial drugs effective against the accompanying gram-positive and gram-negative microflora were considered as the samples of the selective components. Vancomycin from the group of glycopeptides, linezolid from the group of oxazolidinones, and telithromycin from the group of ketolides were chosen. Antibiotics vancomycin and telithromycin in a dose of 0.008 g/dm3, linezolid 0.004 g/dm3 were cho-sen as the selective additive to Drigalski Lactose Agar medium.

scholarly journals Biotechnological peculiarities of microalgae chlorella production and use in poultry

2021 ◽  
Vol 285 ◽  
pp. 03002
Author(s):  
Nadezhda Machneva ◽  
Anna Gneush ◽  
Kirill Ivanov
Keyword(s):  
Nutrient Medium ◽  
Current Paper ◽  
Biomass Growth ◽  
Nutrient Media

The current paper presents data on the features of biotechnological cultivation of chlorella microalgae strain IFR C-111 on different nutrient media. Evaluation of the influence of the illumination period on the rate of biomass growth on the selected nutrient medium has also been presented. There are materials of microalgae suspension influence the effect on the biological resource’s potential of quails.

scholarly journals Suppression of Methane Generation during Methanogenesis by Chemically Modified Humic Compounds

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1140
Author(s):  
Elena Efremenko ◽  
Olga Senko ◽  
Nikolay Stepanov ◽  
Nikita Mareev ◽  
Alexander Volikov ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Nutrient Media ◽  
Potassium Humate ◽  
Methane Generation ◽  
Chemically Modified ◽  
Humic Compounds ◽  
Negative Effect ◽  
The Media ◽  
First Time ◽  
Derivatives Of

The introduction of various concentrations of chemically modified humic compounds (HC) with different redox characteristics into the media with free and immobilized anaerobic consortia accumulating landfill gases was studied as approach to their functioning management. For this purpose, quinone (hydroquinone, naphthoquinone or methylhydroquinone) derivatives of HC were synthesized, which made it possible to vary the redox and antioxidant properties of HC as terminal electron acceptors in methanogenic systems. The highest acceptor properties were obtained with potassium humate modified by naphthoquinone. To control possible negative effect of HC on the cells of natural methanogenic consortia, different bioluminescent analytical methods were used. The addition of HC derivatives, enriched with quinonones, to nutrient media at concentrations above 1 g/L decreased the energetic status of cells and the efficiency of the methanogenesis. For the first time, the significant decrease in accumulation of biogas was reached as effect of synthetic HC derivatives, whereas both notable change of biogas composition towards increase in the CO2 content and decrease in CH4 were revealed. Thus, modification with quinones makes it possible to obtain low-potential HC derivatives with strongly pronounced acceptor properties, promising for inhibition of biogas synthesis by methanogenic communities.

Effects of mineral nutrients on ozone susceptibility of Lemna minor

1971 ◽  
Vol 49 (8) ◽  
pp. 1411-1414 ◽  
Author(s):  
Lyle E. Craker
Keyword(s):  
Nutrient Medium ◽  
Lemna Minor ◽  
Mineral Nutrients ◽  
Nutrient Media ◽  
Nutrient Levels ◽  
Ozone Injury ◽  
Phosphorus And Potassium ◽  
Additional Nitrogen ◽  
Additional Iron

Susceptibility of Lemna minor L. to ozone injury was influenced by the mineral nutrients available to the Lemna plants. Additional nitrogen or additional iron in the nutrient media respectively enhanced or reduced chlorophyll loss of Lemna plants fumigated with ozone. Lemna plants growing on a nutrient medium lacking copper had significantly less injury from ozone fumigation than Lemna plants growing on a complete nutrient medium. There were apparent interactions among phosphorus and potassium nutrient levels in determining the Lemna plant's susceptibility to ozone.

Carcinogenicity of gentian violet, leucogentian violet, malachite green, leucomalachite green, and CI Direct Blue 218

2021 ◽  
Vol 22 (5) ◽  
pp. 585-586
Author(s):  
Frank Le Curieux ◽  
Julia M Gohlke ◽  
Anjoeka Pronk ◽  
Wendy C Andersen ◽  
Guosheng Chen ◽  
...  
Keyword(s):  
Malachite Green ◽  
Gentian Violet ◽  
Direct Blue ◽  
Leucomalachite Green

scholarly journals Phyllanthus wightianusMüll. Arg.: A Potential Source for Natural Antimicrobial Agents

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
D. Natarajan ◽  
R. Srinivasan ◽  
M. S. Shivakumar
Keyword(s):  
Fungal Pathogens ◽  
Antimicrobial Agents ◽  
Shigella Flexneri ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Proteus Vulgaris ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Infective Agent

Phyllanthus wightianusbelongs to Euphorbiaceae family having ethnobotanical importance. The present study deals with validating the antimicrobial potential of solvent leaf extracts ofP. wightianus. 11 human bacterial pathogens (Bacillus subtilis,Streptococcus pneumoniae,Staphylococcus epidermidis,Proteus vulgaris,Pseudomonas aeruginosa,Klebsiella pneumoniae,Salmonella typhimurium, Escherichia coli, Shigella flexneri, Proteus vulgaris, andSerratia marcescens) and 4 fungal pathogens (Candida albicans, Cryptococcus neoformans, Mucor racemosus, andAspergillus niger) were also challenged with solvent leaf extracts usingagar well and disc diffusion methods. Further, identification of the active component present in the bioactive extract was done using GC-MS analysis. Results show that all extracts exhibited broad spectrum (6–29 mm) of antibacterial activity on most of the tested organisms. The results highlight the fact that the well in agar method was more effective than disc diffusion method. Significant antimicrobial activity was detected in methanol extract againstS. pneumoniae(29 mm) with MIC and MBC values of 15.62 μg/mL. GC-MS analysis revealed that 29 bioactive constituents were present in methanolic extract ofP. wightianus,of which 9,12-octadecaenioic acid (peak area 22.82%; RT-23.97) and N-hexadecanoic acid (peak area 21.55% RT-21.796) are the major compounds. The findings of this study show thatP. wightianusextracts may be used as an anti-infective agent in folklore medicine.

scholarly journals Development of the technology for preparation of enzymatic hydrolysate of waste chick embryos

2021 ◽  
Vol 21 (3) ◽  
pp. 200-205
Author(s):  
Yu. S. Ovsyannikov ◽  
M. S. Dursenev
Keyword(s):  
Nutrient Medium ◽  
Mass Fraction ◽  
Chemical Properties ◽  
Chick Embryos ◽  
Nutrient Media ◽  
Enzymatic Hydrolysate ◽  
E Coli ◽  
Physico Chemical ◽  
Growth Properties ◽  
Solid Nutrient Medium

The development of technologies for preparation of protein nutritional bases for microbiological nutrient media, from production waste of mainly readily available or non-food products, is a promising area in biotechnology. Researchers of Vyatka State Agrotechnological University assume that non-food secondary raw materials, such as waste chick embryos (WCEs) used in the production of anti-influenza products, could be used for these purposes, after removal of the virus-containing allantoic fluid. The aim of the study was to develop a technology for preparation of WCE enzymatic hydrolysate (WCEEH), and to evaluate growth properties of the hydrolysate-based solid nutrient medium, using Escherichia coli M-17 and Pseudomonas alcaligenes IP-1 test strains. Materials and methods: the authors offer methodological approaches to obtaining WCEEH and substantiate hydrolysis parameters. The obtained WCEEH was characterised in terms of physico-chemical properties: pH, amine nitrogen, total nitrogen, sodium chloride, degree of protein cleavage. The growth properties of the hydrolysate-based nutrient medium were studied using E. coli M-17 and Ps. alcaligenes IP-1 test strains. Results: the experiments demonstrated the feasibility of performing enzymatic hydrolysis of WCEs, and assessed physico-chemical properties of the prepared WCEEH batches. The study demonstrated the possibility of using the prepared hydrolysate as a component of solid nutrient media for growing the selected test strains. Conclusions: the study substantiated the optimal technological parameters for WCE enzymatic hydrolysis: pH (7.6 ± 0.3), duration (48 ± 2 h), temperature (49 ± 1) °C. The loading of hydrolysis components was optimised: mass fraction of the substrate—500 g/L, mass fraction of the hydrolysing agent—100 g/L. The physico-chemical properties of WCEEH make it suitable for preparation of microbiological media; the hydrolysate-based solid nutrient medium consistently ensures the growth of E. coli M-17 and Ps. alcaligenes IP-1 test strains with standard properties. The growth properties of the experimental medium are comparable to those of the meat-peptone broth-based nutrient medium.

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