THE EFFECT OF ESSENTIAL OIL MIXTURES ON MORTALITIES AND OVIPOSITION DETERRENTS OF Crocidolomia pavonana AND Helopeltis antoniierrents Of Crocidolomia Pavonana And Helopeltis  Antonii

Essential oil, such as citronella, lemongrass, ageratum, and clove are known to be toxic and repel certain pests. Mixing two or more essential oils are expected increasing their insecticidal properties. The experiments aimed to examine the effect of essential oils mixtures (EOs) on mortalities and oviposition deterrents of Crocidolomia pavonana and Helopeltis antonii. The experiments were conducted at the laboratory and green house of the Indonesian Spices and Medicinal Crops Research Institute. The tested mixtures were citronella and clove (CiC); lemongrass and clove (LC); ageratum and clove (AC); individual EO; control 1 (water + emulsifier); control 2 (water) at 0.5% concentration for all treatments. The ratio used were 1:1, 1:2, 1:4, 2:1 and 4:1. Parameters observed were the mortalities of C. pavonana larvae and H. antonii nymphs; and the ovipositions deterrents of both insects. The CiC and LC mixtures gave low mortality (<50%) to C. pavonana larvae, while AC at ratios 1:1, 1:2,1:4 less than 20%, AC 2:1 and 4:1 >60% and the individual ageratum oil caused 77.5% mortalities. The CiC 1:1 and LC (1:1, 1:2, and 4:1) showed >40% mortalities of H. antonii, whereas the AC in all ratios enhanced the nymph mortalities. The number of eggs laid by C. pavonana at CiC and LC in all ratios were around 50-60% lower than individual EO. The mixture of AC 1:4 and 4:1 produced eggs nearly 50% lower than clove treatment. The CiC and LC at 1:1 produced the lowest numbers of egg laid by H. antonii, while AC 1:4 reduced 70% eggs laid compared to individual EO.

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The Effect of Essential Oil Formulas on Mortality and Oviposition Deterrent of Helopeltis antonii

Buletin Penelitian Tanaman Rempah dan Obat ◽

10.21082/bullittro.v28n2.2017.171-180 ◽

2018 ◽

Vol 28 (2) ◽

pp. 171

Author(s):

Tri Lestari Mardiningsih ◽

NFN Ma'mun

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Tween 80 ◽

Oviposition Deterrent ◽

Green House ◽

Citronella Oil ◽

Medicinal Crops ◽

Research Institute

Citronella, clove and lemongrass oils are known to be toxic and repel certain pests. Mixing different essential oils (EOs) in the form of formula is expected to increase the insecticidal properties. The experiments aimed to examine the effect of EOs mixture formulas on mortality and oviposition deterrent of Helopeltis antonii. The study was conducted at the green house of Indonesian Spices and Medicinal Crops Research Institute. A mixture of two EOs citronella + clove (1:1), lemongrass + clove (1:1), individual EO formulas, and their inert solution (mixture of tween 80, turpentine, and teepol) were tested on H. antonii at 5 and 10 ml.l-1 concentrations. Parameters observed were the number of eggs laid, mortality of adults and nymphs of H. antonii. The citronella + clove and lemongrass + clove formula at 10 ml.l-1 deterred the oviposition of H. antonii by 60.18 % and 46.56 % respectively. These formulas also caused adults mortality at 79.17 % and 62.50 %, as well as the nymphs mortality at 87.50 % and 82.50 % respectively. The citronella + clove and lemongrass + clove formulas tested had the same effectiveness compared to the commercial citronella oil formula. Therefore, these two formulas were potential to be tested in field to control H. antonii.

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PENGARUH KOLKHISIN TERHADAP PENAMPILAN LADA P p er nigrum L.) MUTAN DAN ANALISIS PLOIDI

Jurnal Penelitian Tanaman Industri ◽

10.21082/littri.v21n3.2015.125-130 ◽

2016 ◽

Vol 21 (3) ◽

pp. 125

Author(s):

NATALINI NOVA KRISTINA ◽

SITTI FATIMAH SYAHID

Keyword(s):

Genetic Variability ◽

Growth Parameters ◽

Colchicine Treatment ◽

Piper Nigrum ◽

Green House ◽

Ploidy Analysis ◽

Plant Phenotype ◽

Medicinal Crops ◽

Colchicine Solution ◽

Research Institute

ABSTRAKKeragaman genetik plasma nutfah lada (Piper nigrum) di Indonesia rendah sehingga perlu dilakukan peningkatan keragaman. Penelitian dilakukan di Rumah Kaca Balittro Bogor mulai Januari 2012 sampai Juni 2013. Tujuan penelitian adalah untuk meningkatkan keragaman genetik lada varietas Petaling 1 menggunakan mutagen kimia kolkhisin. Biji lada direndam dalam larutan kolkhisin konsentrasi 0; 0,01; 0,03; dan 0,05% selama 4 jam dan disemai pada bak pasir. Masing-masing perlakuan diulang 3 kali dan setiap ulangan terdiri atas 300 biji. Pengamatan dilakukan terhadap persentase perkecambahan dan fenotipe tanaman, persentase tumbuh, tinggi tanaman, serta jumlah ruas dan daun pada umur dua bulan. Selanjutnya, sebanyak 20 individu dari total benih yang tumbuh dipilih berdasarkan rata rata penggabungan dari tanaman terpendek dan tertinggi. Individu terpilih diamati tinggi tanaman serta jumlah ruas dan daun pada umur empat bulan. Untuk melihat ragam genetik dilakukan analisis kandungan DNA dengan flowcytometry. Hasil penelitian menunjukkan bahwa perlakuan kolkhisin 0,01 dan 0,05% menghasilkan persentase perkecambahan benih di persemaian lebih tinggi. Pada lada mutan vegetatif generasi ke-0, perubahan morfologi terindikasi pada konsentrasi 0,03%. Pada generasi mutan hasil perbanyakan/turunan vegetatif pertama perubahan morfologi pada tanaman terjadi pada perlakuan 0,05%. Namun, tidak ada perbedaan yang nyata pada tingkat ploidi lada pada semua perlakuan termasuk kontrol.Kata kunci: Piper nigrum L., ragam genetik, mutan, kolkhisin, fenotip ABSTRACTEffect of Colchicine on the Phenothypic Performance of Pepper (Piper nigrum L.) Mutant and Ploidy analysisGenetic variability of pepper (Piper nigrum) in Indonesia was low, so it was needed to increase its variability. Research was conducted at the green house of Indonesian Spices and Medicinal Crops Research Institute, Bogor from January 2012 to June 2013. The aim of the research was to increase the genetic variability of pepper (Petaling 1) using chemical mutagen colchicine. Seeds of pepper were soaked in colchicine solution with several concentration (0; 0,01; 0,03; and 0,05%) for four hours, and then germinated on sand media. Every treatment consisted of 300 seeds and replicated three times. The parameter observed were germination percentage, plant phenotype, growth percentage, plant hight, number of node and leaves two months after planting. Further, from total seedling growth, 20 individual were selected based on average combined from highest and shortest plant. The selected individual observed their plant height, number of node and leaves on four months. Flowcytometri analysis from the selected seedling was conducted to find interplant genetic variabilities. The result showed that application of colchicin 0,01 and 0,05% performed the fast germination on the nursery compared with control, but no significant differencet on the growth parameters. In the mutant generation 0, the changes on morphology showed on 0,03% and at the first vegetative generation, the changes were indicated in plants from 0,05% of colchicine treatment. Flowcytometri analysis showed no significant differences on ploidi level of all treatments including control.Keywords: Piper nigrum, genetic variability, mutant, cholchicin, phenotype

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EVALUASI KETAHANAN BEBERAPA AKSESI JAMBU METE (Anacardium occidentale L.) TERHADAP HAMA Helopeltis antonii SIGN. (HEMIPTERA: MIRIDAE)

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v10n4.2004.149-153 ◽

2020 ◽

Vol 10 (4) ◽

pp. 149

Author(s):

ANDI MUHAMMAD AMIR ◽

ELNA KARMAWATI ◽

HADAD E. A.

Keyword(s):

Key Words ◽

Block Design ◽

Anacardium Occidentale ◽

Green House ◽

Medicinal Crops ◽

Research Institute

Penelitian evaluasi ketahanan beberapa aksesi jambu mete (Anacardium occidentale L.) terhadap hama Helopeltis antonii Sign. (Hemiptera: Miridae) telah dilaksanakan di Laboratorium Hama dan Penyakit dan Rumah Kasa Balai Penelitian Tanaman Rempah dan Obat (BALITTRO) Bogor, mulai bulan April sampai Desember 2004, bertujuan untuk menguji ketahanan beberapa aksesi jambu mete terhadap H antonii. Perlakuan tcrdiri atas sembilan aksesi jambu mete, yaitu (1) Balakrisnan (B-02), (2) Madura (L3-3), (3) Jatiroto Jambon (III/4-5), (4) Gunung Gangsir 180, (5) Madura (M4-2), (6) Jogya Putih (XII/8), (7) Mojoketo (XIII/8), (8) Tegineneng (A3-2), dan (9) Wonogiri (C6-5). Penelitian terdiri atas (a) preferensi tanpa pilihan, disusun dalam rancangan acak kelompok (RAK) diulang 5 kali, dan (b) preferensi dengan pilihan, disusun dalam rancangan acak kelompok (RAK) diulang 3 kali. Hasil penelitian menunjukkan bahwa jambu mete aksesi Mojokerto (XIII-8) dan Balakrisnan (B-02) merupakan aksesi jambu mete tahan dan toleran terhadap hama //. antonii.Kata kunci: Anacardium occidentale L, ketahanan, hama, Helopeltis antonii ABSTRACT The evaluation of resistances of some cashew lines (Anacardium occidentale I,.) to Helopeltis Antonii Sign. (Hemiptera: Miridae)The research on the evaluation of resistances of some cashew lines (Anacardium occidentale L.) to Helopeltis antonii Sign. (Hemiptera: Miridae) was conducted in the Pest and Diseases Laboratory and Green House Indonesian Spice and Medicinal Crops Research Institute Bogor, from April to December 2004, to test the resistances of some cashew lines to H. antonii. The treatment consisted of nine cashew lines that is, (1) Balakisnan (B-02), (2) Madura (L3-3), (3) Jatiroto Jambon (I1I/4-5), (4) Gunung Gangsir 180, (5) Madura (M4-2), (6) Jogya Putih (XII/8), (7) Mojokerto (XIII/8), (8) Tegineneng (A3-2), and (9) Wonogiri (C6-5). The research consisted of, (a) preferences without choice, compiled in a randomized completely block design (RCBD) replicated 5 times, and (b) preferences with choice, compiled in a randomized completely block design (RCBD) replicated 3 times. The result indicated that cashew lines of Mojoketo (XIII-8) and Balakrisnan (B-02) were resistant and tolerant to H. antonii.Key words: Cashew, Anacardium occidentale L., resistance, pest. Helopeltis antonii

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Insecticidal Activity of Powder and Essential Oils of Vepris heterophylla (Rutaceae) and Syzygium aromaticum (Mytaceae) Towards Callosobruchus maculatus F. walp (Coleoptera: Bruchidae) on Post-Harvest Vigna unguiculata (Fabaceae) in the Far-North Region of Cameroon

Journal of Experimental Agriculture International ◽

10.9734/jeai/2021/v43i330661 ◽

2021 ◽

pp. 98-112

Author(s):

Matseu Sakou Gerardine Noël ◽

Ngatanko Illiassa ◽

Kouninki Habiba ◽

Nukenine Nchiwan Elias

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Direct Contact ◽

Callosobruchus Maculatus ◽

Individual Treatment ◽

Syzygium Aromaticum ◽

Flower Buds ◽

Indirect Contact ◽

Cowpea Seeds ◽

The Individual

The bioinsecticidal effect of powders and essential oils of S. aromaticum (L.) flower buds and V. heterophylla (Engl.) leaves against adult cowpea weevil C. maculatus was studied. Powders were tested by direct contact only while essential oils were tested by direct contact and indirect contact (inhalation and repellency). In 500 mL glass jars, the individual and combined powders were applied to 100 g of cowpea seeds at 0.5 g, 1 g, 1.5 g and 2 g doses for both leaves and flower buds of V. heterophylla and S. aromaticum respectively. The essential oils were also applied individually and after equilibrium combination on 50 g of cowpea at 4 µL/mL, 8 µL/mL, 12 µL/mL, 16 µL/mL. All the infestations were then achieved by adding 20 bruchids of 48 h old. The essential oil obtained was purified and analyzed with Gas Chromatography and Mass Spectrometry (GC-MS). 72 h after the individual treatment, 100% mortality was obtained at 2 g dose for V. heterophylla and 0.5 g dose for S. aromaticum compared to the control jars (1%). The combined powders were found to be more effective with 100% of mortality at a dose 1 g after 48 h of exposure. For essential oils, 100% and 90% of mortality were obtained respectively at 8 µL/mL dose for S. aromaticum (160 µL/kg) and 16 µL/mL dose for V. heterophylla (320 µL/kg) 72 h after treatment. In addition, the essential oil of S. aromaticum showed relatively higher repellant properties with an average repellency percentage of PR = 89.37% than that of V. heterophylla (PR = 70.62%). 100% of mortality was induced after inhalation of essential oils of S. aromaticum and V. heterophylla at doses 10 µL and 40 µL respectively after 72 h of exposure time. The results obtained from the GC-MS showed that the major components in the essential oil of S. aromaticum were eugenol (83.40%) although that of V. heterophylla were Spathulenol (23.66%), Beta-Caryophyllene oxide (16.46%) and 6-Isopropenyl-4 (16.30%). The powders and essential oils of the tested plants applied individually or after equilibrium combination showed interesting prospects for controlling Callosobruchus maculatus and effectively preserving cowpea seeds in storage warehouses.

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In vitro antifungal activity of Myrcia ovata essential oils and their major compounds against pathogens of citrus, sweet potato, and coconut

Bioscience Journal ◽

10.14393/bj-v35n6a2019-42425 ◽

2019 ◽

Vol 35 (6) ◽

Author(s):

Larissa Alves Secundo White ◽

Arie Fitzgerald Blank ◽

Paulo Roberto Gagliardi ◽

Maria de Fátima Arrigoni-Blank ◽

Daniela Aparecida de Castro Nizio ◽

...

Keyword(s):

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Sweet Potato ◽

Colletotrichum Acutatum ◽

In Vitro Tests ◽

Minimum Fungicidal Concentration ◽

The Individual ◽

Nerolic Acid

Myrcia ovata, an endemic species to the Brazilian Atlantic Forest, presents antifungal properties. The phytopathogens Colletotrichum acutatum, Plenodomus destruens, and Thielaviopsis paradoxa are responsible for the diseases citrus postbloom fruit drop, sweet potato foot rot, and coconut stem bleeding, respectively. The antifungal activity of the essential oils of five M. ovata chemotypes (MYRO-159, nerolic acid chemotype; MYRO-180, nerolic acid + linalool chemotype; MYRO-388, geraniol chemotype; MYRO-157, citral + (E)-nerolidol chemotype; and MYRO-174, isopulegol + linalool chemotype), four major compounds (nerolic acid, nerolic acid + linalool, geraniol, and citral + (E)-nerolidol), and three pure compounds (citral, (E)-nerolidol, and linalool) against the fungi C. acutatum, P. destruens, and T. paradoxa were evaluated. For this, in vitro tests were conducted in a completely randomized design with three replications, testing concentrations (v/v) ranging from 0.01 to 1.0 μL.mL-1. All treatments presented toxicity at different levels to the three fungi. For C. acutatum, the essential oil from the individual MYRO-180 (nerolic acid + linalool chemotype) and its major compound showed the lowest Minimal Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of 0.03 and 0.1 µL.mL-1, respectively. For P. destruens, the essential oil from the individual MYRO-159 (nerolic acid chemotype) presented the lowest MIC of 0.05 μL.mL-1. The nerolic acid + linalool chemotype and its major compound presented an MFC of 0.07 μL.mL-1. For T. paradoxa, the major compound citral + (E)-nerolidol stood out with the lowest MIC and MFC of 0.03 and 0.2 µL.mL-1, respectively. Linalool presented the lowest toxicity to the three tested fungi.

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Comparison of Czech hop cultivars based on their contenst of secondary metabolites

Czech Journal of Food Sciences ◽

10.17221/65/2010-cjfs ◽

2010 ◽

Vol 28 (No. 4) ◽

pp. 309-316 ◽

Cited By ~ 18

Author(s):

L. Jelínek ◽

M. Šneberger ◽

M. Karabín ◽

P. Dostálek

Keyword(s):

Secondary Metabolites ◽

Essential Oil ◽

Essential Oils ◽

Dichotomous Key ◽

Bitter Acids ◽

The Individual ◽

Varietal Differentiation

Seven Czech hop varieties (dry hop cones) coming from the harvest of 2008 (Agnus, Bor, Harmonie, Premiant, Rubín, Sládek, and Saaz) were compared for their composition depending on their varietal differentiation. These cultivars were analysed for the contents of α- and β-bitter acids analogues, essential oils, and polyphenols. Hop essential oil constituents significantly contribute to the individual hop varieties. The dichotomous key for the authentication of Czech hop varieties was established based on some characteristic varietal markers.

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Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

Phytothérapie ◽

10.3166/phyto-2019-0150 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S155-S163 ◽

Cited By ~ 2

Author(s):

S. Mehalaine ◽

O. Belfadel ◽

T. Menasria ◽

A. Messaili

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Rosmarinus Officinalis ◽

Salvia Officinalis ◽

Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

Revista de Chimie ◽

10.37358/rc.19.6.7266 ◽

2019 ◽

Vol 70 (6) ◽

pp. 2015-2020

Author(s):

Silvia Robu ◽

Aurelia Romila ◽

Olimpia Dumitriu Buzia ◽

Adrian Florin Spac ◽

Camelia Diaconu ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Plant Material ◽

Mobile Phase ◽

Chromatographic Method ◽

Salvia Officinalis ◽

Salvia Officinalis L ◽

Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

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Review of the Essential Oil Composition of Iranian Endemic and Native Taxa of Apiaceae (Umbelliferae)

Current Organic Chemistry ◽

10.2174/1385272824999200513103632 ◽

2020 ◽

Vol 24 (9) ◽

pp. 909-1009

Author(s):

Maryam Akaberi ◽

Zahra Tayarani-Najaran ◽

Iraj Mehregan ◽

Javad Asili ◽

Amirhossein Sahebkar ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Essential Oil Composition ◽

Present Review ◽

Aromatic Plants ◽

Oil Composition

One of the most important families of Iranian flora is Apiaceae (Umbelliferae). Most of the species of this family are aromatic plants and rich in essential oils with diverse structures. In the present review, the essential oil composition of 63 genera comprising 141 Apiaceae (66.4% native 33.6% endemic) is summarized.

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Chemical variability and antioxidant activities of the essential oils of the aerial parts of Ammoides verticillata and the roots of Carthamus caeruleus and their synergistic effect in combination

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207323666200606213057 ◽

2020 ◽

Vol 23 ◽

Author(s):

Imane Rihab Mami ◽

Noria Merad-Boussalah ◽

Mohammed El Amine Dib ◽

Boufeldja Tabti ◽

Jean Costa ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Natural Food ◽

Chemical Compositions ◽

Chemical Variability ◽

Ammoides Verticillata

Aim and Objective: Oxidative stress is implicated in the development and progression of many disease. Some of appropriate actions that could be initiated to taken to resolve the problem of these diseases are search for new antioxidant substances isolated from plants. The aims of this study were to study the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8 locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and in combinations. Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies variations of the essential oil compositions were discussed using principal component analysis (PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical scavenging activity and β-carotene bleaching test. Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol (12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide (86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll EOs. A direct correlation between the altitudes, climate and the chemical compositions was evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant activity. In binary mixture, the interaction both Coll Eos and between oils rich of thymol and/or carvacrol with carlina oxide produced the best synergistic effects, compared to individual essential oils and the synthetic antioxidant (BHT). Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a natural food preservative and alternative to chemical antioxidants.

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