INDUKSI TUNAS TABAT BARITO (Ficus deltoidea JACK) SECARA IN VITRO MENGGUNAKAN BENZIL ADENIN (BA) DAN NAPHTHALENE ACETIC ACID (NAA)

ABSTRAK Tabat barito (Ficus deltoidea Jack), merupakan salah satu tanaman obat yang dikategorikan langka dan digunakan sebagai bahan afrodisiak wanita. Perbanyakan tanaman secara in vitro dilakukan untuk mendapatkan bahan tanaman dalam jumlah banyak. Penelitian bertujuan untuk mendapatkan media terbaik tabat barito dan telah dilakukan di laboratorium kultur jaringan Plasma Nutfah dan Pemuliaan Balittro, pada bulan Januari sampai dengan Desember 2007. Penelitian ini dilakukan dalam tiga tahap, yaitu : 1) respon tunas pada media perbanyakan, menguji media multiplikasi tunas dengan media sitokinin tunggal yaitu : MS + Benzil Adenin (BA) 0,5; 1,0; 1,5 dan 2,0 mg/l; tahap 2) respon tunas pada media kombinasi sitokinin dan auksin, yaitu : MS + BA 0,5 mg/l + NAA 0,1 mg/l; MS + BA 0,5 mg/l + NAA 0,5 mg/l; MS + BA 1,0 mg/l + NAA 0,1 mg/l dan MS + BA 1,0 mg/l + NAA 0,5 mg/l. Tahap 3) Daya multiplikasi dan penampilan tunas setelah subkultur pada media yang sama. Masingmasing percobaan disusun dengan rancangan acak lengkap, dan terdiri atas 5 ulangan. Parameter pengamatan meliputi jumlah tunas, tinggi tunas dan jumlah ruas serta penampilan visual. Hasil penelitian menunjukkan bahwa pada tahap pertama, penggunaan media MS + BA 0,5 menghasilkan jumlah tunas yang paling banyak dibandingkan yang lain, tetapi tidak berbeda nyata pada jumlah ruas dan tinggi eksplan. Sementara pada tahap dua, jumlah tunas terbaik didapat pada media dengan auksin rendah baik pada kombinasi sitokinin rendah dan tinggi. Sementara untuk jumlah ruas media terbaik adalah media dengan konsentrasi BA tinggi yang dikombinasi dengan NAA. Untuk tinggi tunas, media terbaik adalah MS + BA 1,0 mg/l + NAA 0,5 mg/l, tapi ditemukan eksplan yang menguning. Pada tahap ketiga, dari hasil subkultur kembali terlihat bahwa tunas yang bersumber dari pucuk pertumbuhannya baik sementara tunas yang berasal dari ruas ke-2 dan 3 sebagian menguning. Kata kunci : Ficus deltoidea Jack, tunas, induksi, in vitro, BA, NAAABSTRACT In vitro shoot induction of Mistleteo fig (Ficus deltoidea Jack) in Murashige & Skoog (MS) media with addition of BA and NAA Mistleteo fig (Ficus deltoidea) is one of endangered medicinal plants and used for female aphrodisiac. In vitro multiplication of the plant was done to find a number of shoots. This experiment was conducted in tissue culture laboratory of Germplasm and Breeding of IMACRI from January to December 2007, and aimed to find best media for shoot multiplication. This experiment was carried out in three steps: step 1) shoot respon in multiplication media using single cytokinin : MS + BA (0.5; 1.0; 1.5 and 2 mg/l); step 2) shoot respon in multiplication media of combined cytokinin and auxin : MS + BA 0.5 mg/l + NAA 0.1mg/l; MS + BA 0.5 mg/l + NAA 0.5 mg/l; MS + BA 1.0 mg/l + NAA 0.1 mg/l and MS + BA 1.0 + NAA 0.5 mg/l; and step 3) viability and visualization of the shoots after subcultured in the same media. The experiment was arranged using completely randomized design with 5 replicates. The parameters observed were of shoots and nodes, shoot height and performance. The results in the first step showed that MS + BA 0.5 mg/l media resulted in the highest number of shoots, but they were not significantly different in the number of nodes and shoots height. In the second step, highest number of shoots was found using low concentration of auxin combined with low and high concentration of cytokinin. Best medium for number of nodes was MS with high concentration of BA combined with NAA. For shoot height, the best medium was MS + BA 0.1 mg/l + NAA 0.5 mg/l, but the shoots turned yellow. In the third step, after subcultured, the shoots originated from plant tips performed well, however, those taken from second and third inter nodes partially turned yellow. Key words : Ficus deltoidea Jack, shoot, induction, in vitro, BA, NAA

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MULTIPLIKASI TUNAS PUCUK TOMAT (Lycopersicum esculentum MILL) DENGAN MENGGUNAKAN BENZYL AMINO PURINE (BAP) DAN NAPHTALENE ACETIC ACID (NAA) SECARA IN VITRO

10.31219/osf.io/j75gd ◽

2019 ◽

Author(s):

F. Fathurrahman

Keyword(s):

Acetic Acid ◽

Shoot Multiplication ◽

Shoot Height ◽

Factor B ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Benzyl Amino Purine ◽

Number Of Shoots

Research with the title shoot multiplication shoots tomatoes (Lycopersicumesculentum mill) using the Benzyl Amino Purine (BAP) dan Naphtalene Acetic Acid(NAA) in vitro have been conducted at the Laboratory of Biotechnology Faculty ofAgriculture, Islamic University of Riau, Pekanbaru. This research has been carried outfor three months carried out startingfrom November 2010 to February 2011. This studyaims to determine the effect of a single interaction between the administration and plantgrowth regulators BAP and NAA on shoot multiplication of in tomato shoots vitro.Rancangan used in this study was completely randomized design (CRD) in factorialwhich consist of two factors. The first factor is factor B (concentration of BAP) withfour standard treatments are: B0 (0 ppm), B1 (1 ppm), B2 (2 ppm), B3 (3 ppm).Thesecond factor is the factor (the concentration of NAA) with four standard treatments,including: N0 (0 ppm), N1 (0.1 ppm), N2 (0.5 ppm), and N3 (1 ppm), to obtain 16combined treatment with three replications. Parameters observed, namely: age emergedshoots, number of shoots, shoot height, the percentage of growing shoots, roots andgrowing percentage of the number of explants forming callus. The data was statisticallyanalyzed the results of observations, when the F calculated is greater than the F table,followed by a further test of honest real difference (HRD) 5%. From the results ofresearch in the interaction of BAP and NAA effect on the parameters of high-shoots bytreatment tebaik B1N0 namely 6.16 cm. BAP singly significantly affect the parametersage appears buds (days) and the percentage grows shoots with the best treatment B2 (2ppm), shoot height with the best treatment B0 (0 ppm), the number of shoots (the fruit)with the best treatment B3 (3 ppm). singly whereas NAA significantly affect theparameters age appears shoots (day) and high-shoots with the best treatment N0 (0ppm), and the percentage grows roots with the best treatment N3 (1 ppm).

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THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

AGROLAND The Agricultural Sciences Journal ◽

10.22487/j24077593.2018.v5.i2.11121 ◽

2019 ◽

Vol 5 (2) ◽

pp. 67

Author(s):

Mardiana Mardiana ◽

Zainuddin Zainuddin ◽

Mahfudz Mahfudz ◽

Hawalina Hawalina

Keyword(s):

Shoot Growth ◽

Good Condition ◽

Kiwi Fruit ◽

Physiological Maturity ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Time Required ◽

Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

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Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

JURNAL BIOS LOGOS ◽

10.35799/jbl.11.2.2021.32674 ◽

2021 ◽

Vol 11 (2) ◽

pp. 114

Author(s):

Nurkapita Nurkapita ◽

Riza Linda ◽

Zulfa Zakiah

Keyword(s):

Tissue Culture ◽

Zea Mays ◽

Acetic Acid ◽

Seed Extract ◽

Naphthalene Acetic Acid ◽

Shoot Height ◽

Culture Techniques ◽

Corn Seed ◽

Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn seed extract; NAA

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Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201967010009 ◽

2019 ◽

Vol 67 (1) ◽

pp. 9-18

Author(s):

Enik Akhiriana ◽

Samanhudi ◽

Ahmad Yunus

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Tribulus Terrestris ◽

Emergence Time ◽

Shoot Height ◽

Randomized Design ◽

Number Of Leaves ◽

Indole 3 Acetic Acid ◽

Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

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Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽

10.15575/1344 ◽

2017 ◽

Vol 4 (2) ◽

pp. 97-109

Author(s):

Lamro Purba ◽

Erni Suminar ◽

Denny Sobardini ◽

Wieny Rizky ◽

Syariful Mubarok

Keyword(s):

Tissue Culture ◽

Leaf Number ◽

Shoot Induction ◽

Seed Technology ◽

Randomized Design ◽

Rooting Media ◽

Completely Randomized Design ◽

Four Levels ◽

Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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RESPONSE OF TURMERIC SHOOT EXPLANT AFTER CYTOKININ WITH IN VITRO

Jurnal Penelitian Saintek ◽

10.21831/jps.v24i2.20475 ◽

2019 ◽

Vol 24 (2) ◽

pp. 143-152

Author(s):

Marhan Nurullia ◽

Erni Suminar Suminar ◽

Anne Nurani

Keyword(s):

Tissue Culture ◽

Root Length ◽

Air Flow ◽

T Test ◽

Shoot Height ◽

Best Response ◽

Randomized Design ◽

Completely Randomized Design ◽

Laminar Air Flow

This study was aimed at determining the response of turmeric shoot explants after the provision of various types and concentrations of cytokinins in vitro. This experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University from January to April 2018. The data were analyzed using T-Test. The experimental method used in this research was Completely Randomized Design (CRD). Explant planting was carried out in Laminar Air Flow. The experiment consisted of 7 treatments consisting of 4 replications and each test consisted of 4 units. Observation of this experiment was carried out for 12 MST. The main observations were made on the data that were tested statistically namely the percentage of explant growing shoots, percentage of explant growing roots, shoot height, number of tuns, number of roots and root length. The treatments consisted of Control, 2.5 mg L-1 BAP, 5 mg L-1 BAP, 0.5 mg L-1 TDZ, 1 mg L-1 TDZ, 0.01 mg L-1 Zeatin and 0.1 mg L Zeatin -1. The results show that the treatment of 1 mg L-1 TDZ shows the best response to the growth of turmeric explants by increasing the number of turmeric shoot explants than the others.RESPONS EKSPLAN TUNAS KUNYIT SETELAH SITOKININ SECARA IN VITROTujuan dari penelitian ini yaitu untuk melihat respons eksplan tunas kunyit terhadap pemberian berbagai jenis dan konsentrasi sitokinin secara in vitro. Percobaan ini dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Padjadjaran dari bulan Januari sampai April 2018. Hasil percobaan dianalisis dengan Sample T-Test. Metode percobaan yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL). Penanaman eksplan dilakukan di dalam Laminar Air Flow. Percobaan terdiri dari 7 perlakuan sebanyak 4 ulangan dan setiap ulangan terdiri dari 4 unit. Pengamatan percobaan ini dilakukan selama 12 MST. Pengamatan utama dilakukan terhadap data-data yang diuji secara statistik yakni persentase eksplan tumbuh tunas, persentse eksplan tumbuh akar, tinggi tunas, jumlah tunas, jumlah akar dan panjang akar. Perlakuan terdiri dari Kontrol; 2,5 mg L-1 BAP; 5 mg L-1 BAP; 0,5 mg L-1 TDZ; 1 mg L-1 TDZ; 0,01 mg L-1 Zeatin; dan 0,1 mg L-1 Zeatin. Hasil penelitian menunjukkan bahwa perlakuan 1 mg L-1 TDZ menunjukkan respons yang lebih baik terhadap pertumbuhan eksplan kunyit dengan meningkatkan jumlah tunas eksplan tanaman kunyit daripada yang lainnya.

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Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung

Jurnal AgroBiogen ◽

10.21082/jbio.v8n3.2012.p89-96 ◽

2016 ◽

Vol 8 (3) ◽

pp. 89

Author(s):

Iswari S Dewi ◽

Anggi Nindita ◽

Bambang S. Purwoko ◽

Darda Efendi

Keyword(s):

Growth Regulator ◽

Woody Plants ◽

De Novo ◽

Culture Media ◽

Shoot Induction ◽

Indirect Organogenesis ◽

Randomized Design ◽

Callus Initiation ◽

Completely Randomized Design

Propagation through tissue culture of plant species with rich secondary metabolites such as Jatropha curcas L. is difficult to obtain. However, once established, it can be used as one of the alternatives to supply uniform propagules. The effects of auxin and cytokinin on the regulation of de novo woody plants shoot development have been studied through shoot induction, differentiation and development. The objective of this research was to identify explant and suitable culture media for in vitro shoot induction through indirect organogenesis. Factorial experiment was arranged in a completely randomized design, replicated 20 times. The first factor was explants, i.e. cotyledons and hypocotyls. The second factor was MS media containing combination of plant growth regulator IAA (0, 0.05, and 0.1 mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of the experiment showed that the fastest callus initiation was achieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explants were cultured. Shoots with leaves can be induced from both cotyledons and hypocotyls. However, hypocotyls gave more shoots and leaves than cotyledons when cultured in MS + IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotyls and cotyledons were successfully rooted in MS medium without any growth regulator.

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Induksi Kalus dan Regenerasi Tiga Genotipe Tomat (Solanum lycopersicon L.) melalui Kultur Antera

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.7.2.75-82 ◽

2016 ◽

Vol 7 (2) ◽

pp. 75

Author(s):

Ratna Ningsih ◽

Bambang S. Purwoko ◽

Muhamad Syukur ◽

Iswari S. Dewi

Keyword(s):

Anther Culture ◽

Callus Induction ◽

Solanum Lycopersicon ◽

Shoot Induction ◽

Randomized Design ◽

Hybrid Variety ◽

Completely Randomized Design ◽

Tomato Genotypes

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del> genotype<ins cite="mailto:Windows%207" datetime="2016-10-31T15:07">s</ins>. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% while in Tora was 2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% and Ratna was <del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del>0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%.Keywords: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah <del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>tomat varietas <ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>% lebih tinggi dibandingkan Tora (2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%) dan Ratna (<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:09"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins>%).Kata kunci: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auksin, in vitro, media, sitokinin, tomat

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Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP

Nusantara Bioscience ◽

10.13057/nusbiosci/n110209 ◽

2019 ◽

Vol 11 (2) ◽

Author(s):

Kadek dwipayani Lestari ◽

NI WAYAN DESWINIYANTI ◽

IDA AYU ASTARINI ◽

LUH MADE ARPIWI

Keyword(s):

Plant Growth ◽

Leaf Explants ◽

Lilium Longiflorum ◽

Culture Technique ◽

Naphthalene Acetic Acid ◽

Shoot Induction ◽

Leaf Culture ◽

Randomized Design ◽

Easter Lily ◽

Completely Randomized Design

Abstract. Lestari NKD, Deswiniyanti NW, Astarini IA, Arpiwi LM. 2019. Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP. Nusantara Bioscience 11: 162-165. Lilium longiflorum Thunb., an Easter lily, is a common ornamental plant used as potted plant, cut flower, cosmetic and medicine. The research on tissue culture technique to induce shoots and callus is expected to increase the yield of lilies quickly and efficiently. This study aims to determine the effect of plant growth regulator combination of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on the development of leaf culture and the best concentration for callus induction and shoots of lily plants. This study was conducted in a completely randomized design using leaf explants in Vacin and Went medium, with combination of NAA and BAP (0; 0.5; 1 mg..L-1 ) as the treatments. The result of the eight-week observations shows that the plant growth regulators significantly affected either in days initiation callus, days initiation of shoot, percentage of callus, percentage of shoot, diameter of callus, no of shoot and length of shoot. The combination of 1 mg.L-1 NAA and BAP had the best effect in the percentage of explants forming shoots (100%), means number of shoots (5.8), and means length of shoot (11.6 cm).

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In Vitro Response of Protocorm Grammatophyllum stapeliiflorum (Teijsm. & Binn.) J.J.Sm. Orchid in Growth on Several Media Composition

JURNAL BIOLOGI UNAND ◽

10.25077/jbioua.7.2.118-125.2019 ◽

2019 ◽

Vol 7 (2) ◽

pp. 118

Author(s):

Ranny Wirmasari ◽

Mayta Novaliza Isda

Keyword(s):

Growth And Development ◽

Coconut Water ◽

Media Composition ◽

Randomized Design ◽

In Vitro Response ◽

Completely Randomized Design ◽

Original Habitat ◽

Test Treatment ◽

Number Of Shoots

Family Orchidaceae has about 800 genera which are already difficult to find and almost extinct, including the orchid genus Grammatophyllum. A species of rare orchids, Grammatophyllum stapeliiflorum, is hard to find in original habitat. The addition of Growmore, BAP and 15% coconut water is expected to increase the growth and development of the G. stapeliiflorum orchid protocorms. This study use a Completely Randomized Design, consisting of 6 treatments (control, 1 and 3 mg/l BAP, 1 and 3 g/l Growmore, and 150 ml/l coconut water) in MS media with 5 replicates with observation for 4 weeks after planting. The results showed did not differ markedly in the number of protocorms, number of shoots and number of browning protocorms, but differ markedly in the parameter protocorm color based on DMRT test. Treatment of 3 mg/l BAP gives the best results on the number of shoots 2,60 protocorms and number of browning protocormss at the least amount 0,80 protocorms. The number of protocorms most widely on the treatment of 1 g/l Growmore 14,40 protocorms. Protocorms color is best found in 3 g/l Growmore with green color. This research managed to multiply the number of protocorms and induces protocorms shoots from Grammatophyllum stapeliiflorum orchid.

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