Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung

Propagation through tissue culture of plant species with rich secondary metabolites such as Jatropha curcas L. is difficult to obtain. However, once established, it can be used as one of the alternatives to supply uniform propagules. The effects of auxin and cytokinin on the regulation of de novo woody plants shoot development have been studied through shoot induction, differentiation and development. The objective of this research was to identify explant and suitable culture media for in vitro shoot induction through indirect organogenesis. Factorial experiment was arranged in a completely randomized design, replicated 20 times. The first factor was explants, i.e. cotyledons and hypocotyls. The second factor was MS media containing combination of plant growth regulator IAA (0, 0.05, and 0.1 mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of the experiment showed that the fastest callus initiation was achieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explants were cultured. Shoots with leaves can be induced from both cotyledons and hypocotyls. However, hypocotyls gave more shoots and leaves than cotyledons when cultured in MS + IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotyls and cotyledons were successfully rooted in MS medium without any growth regulator.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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EFFECT OF CYTOKININS ON IN VITRO SHOOT PROLIFERATION OF OLIVE CULTIVARS “EARLIK” AND “BARI ZAITOON-2”

World Journal of Biology and Biotechnology ◽

10.33865/wjb.003.02.0156 ◽

2018 ◽

Vol 3 (2) ◽

pp. 199

Author(s):

Sharmin Ashraf ◽

Ayesha Manzoor ◽

Bushra Zulfiqar ◽

Muhammad A. Tariq

Keyword(s):

Woody Plants ◽

Shoot Proliferation ◽

Treatment Results ◽

Effective Technique ◽

Randomized Design ◽

Olive Cultivars ◽

Micro Propagation ◽

Completely Randomized Design ◽

Disease Free

In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same concentration had more shoot length (9.10 cm) and number of shoots per explant (3.67). Thus, it was concluded that the findings of these results will help in the future for developing shoot proliferation protocols for other olive cultivars. Moreover, these protocols can also be further be used in other woody plants micro propagation

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In vitro development of Encyclea cordigera in different concentrations of honey

Ciência Rural ◽

10.1590/0103-8478cr20150046 ◽

2015 ◽

Vol 46 (4) ◽

pp. 590-592 ◽

Cited By ~ 2

Author(s):

Cibele Mantovani ◽

Kathia Fernandes Lopes Pivetta

Keyword(s):

Culture Media ◽

Leaf Length ◽

In Vitro Development ◽

Randomized Design ◽

Sucrose Medium ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Set Up ◽

Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

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Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽

10.15575/1344 ◽

2017 ◽

Vol 4 (2) ◽

pp. 97-109

Author(s):

Lamro Purba ◽

Erni Suminar ◽

Denny Sobardini ◽

Wieny Rizky ◽

Syariful Mubarok

Keyword(s):

Tissue Culture ◽

Leaf Number ◽

Shoot Induction ◽

Seed Technology ◽

Randomized Design ◽

Rooting Media ◽

Completely Randomized Design ◽

Four Levels ◽

Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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Induksi Kalus dan Regenerasi Tiga Genotipe Tomat (Solanum lycopersicon L.) melalui Kultur Antera

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.7.2.75-82 ◽

2016 ◽

Vol 7 (2) ◽

pp. 75

Author(s):

Ratna Ningsih ◽

Bambang S. Purwoko ◽

Muhamad Syukur ◽

Iswari S. Dewi

Keyword(s):

Anther Culture ◽

Callus Induction ◽

Solanum Lycopersicon ◽

Shoot Induction ◽

Randomized Design ◽

Hybrid Variety ◽

Completely Randomized Design ◽

Tomato Genotypes

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del> genotype<ins cite="mailto:Windows%207" datetime="2016-10-31T15:07">s</ins>. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% while in Tora was 2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% and Ratna was <del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del>0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%.Keywords: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah <del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>tomat varietas <ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>% lebih tinggi dibandingkan Tora (2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%) dan Ratna (<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:09"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins>%).Kata kunci: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auksin, in vitro, media, sitokinin, tomat

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PENGARUH EKSTRAK Sargassum cristaefolium PADA MULTIPLIKASI Dendrobium antennatum Rchb.f SECARA IN VITRO

Bioscientist : Jurnal Ilmiah Biologi ◽

10.33394/bjib.v8i1.2672 ◽

2020 ◽

Vol 8 (1) ◽

pp. 18

Author(s):

Faisal Ansyarif ◽

Mursal Ghazali ◽

Aida Muspiah ◽

Rina Kurnianingsih

Keyword(s):

Culture Media ◽

Positive Control ◽

Negative Control ◽

Growth Media ◽

Extract Concentration ◽

Tissue Culture Media ◽

Randomized Design ◽

Completely Randomized Design ◽

Natural Cytokinin

The purpose of this study was to determine the potential and concentration of Sargassum cristaefolium extract as a natural cytokinin in tissue culture media of Dendrobium antennatum Rchb.f. This study is experimental with a completely randomized design, using several extract concentrations compared with the positive control (BAP 1.5 ppm) and negative control (MS0 media). Extract concentrations used 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm. Based on analysis of variance (ANOVA), the effect of Sargassum cristaefolium extract on the growth media significant on all parameters. Sargassum cristaefolium extracts caused different responses at certain levels of concentration. Extract concentration of 10 ppm was able to initiate the highest number of shoots and leaves compared to other extract concentrations, where as the concentration 20 ppm was able to accelerate and increase root growth.

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Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Pemberian ZPT 2,4-D (Dichlorophenoxy Acetid Acid)

Natural Science Journal of Science and Technology ◽

10.22487/25411969.2019.v8.i2.13538 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Siti RLR Idris ◽

Asri Pirade Paserang

Keyword(s):

Solanum Tuberosum ◽

Callus Induction ◽

Culture Media ◽

Callus Formation ◽

Solanum Tuberosum L ◽

Randomized Design ◽

Completely Randomized Design

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

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Induction of Barangan Banana shoot (Musa acuminata L.) from North Nias through Tissue Culture by giving 2,4-D and Kinetin

JURNAL BIOSAINS ◽

10.24114/jbio.v1i2.2780 ◽

2016 ◽

Vol 1 (2) ◽

pp. 1

Author(s):

Destarius Zebua ◽

Suci Rahayu ◽

Saleha Hanum

Keyword(s):

Tissue Culture ◽

Growth Regulators ◽

Growth Regulator ◽

Musa Acuminata ◽

Basal Part ◽

Banana Weevil ◽

Shoot Induction ◽

Randomized Design ◽

Completely Randomized Design ◽

Basal Position

The research on induction of banana barangan shoot (Musa acuminata L.) from North Nias through tissue culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue Culture University of North Sumatera from May until October 2014. The main objective of this research was to know the explants of banana weevil in apical and basal position with growth regulator 2,4-D with kinetin which are able to induce shoots from callus initials. On this research, the treatment tested for induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1 mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was designed using Completely Randomized Design (CRD) two factorial with repetition of experiment. The results of research showed that the interaction between concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days) forming the shoot derived from explant basal part. The concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for 3.00 and average number of highest shoots for 1.50 cm. Keywords: Musa acuminata, weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin.

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INDUKSI TUNAS TABAT BARITO (Ficus deltoidea JACK) SECARA IN VITRO MENGGUNAKAN BENZIL ADENIN (BA) DAN NAPHTHALENE ACETIC ACID (NAA)

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v15n1.2009.33-39 ◽

2020 ◽

Vol 15 (1) ◽

pp. 33

Author(s):

NATALINI NOVA KRISTINA

Keyword(s):

Second Step ◽

Naphthalene Acetic Acid ◽

Shoot Induction ◽

High Concentration ◽

Shoot Height ◽

Ficus Deltoidea ◽

Randomized Design ◽

Completely Randomized Design ◽

Number Of Shoots

ABSTRAK Tabat barito (Ficus deltoidea Jack), merupakan salah satu tanaman obat yang dikategorikan langka dan digunakan sebagai bahan afrodisiak wanita. Perbanyakan tanaman secara in vitro dilakukan untuk mendapatkan bahan tanaman dalam jumlah banyak. Penelitian bertujuan untuk mendapatkan media terbaik tabat barito dan telah dilakukan di laboratorium kultur jaringan Plasma Nutfah dan Pemuliaan Balittro, pada bulan Januari sampai dengan Desember 2007. Penelitian ini dilakukan dalam tiga tahap, yaitu : 1) respon tunas pada media perbanyakan, menguji media multiplikasi tunas dengan media sitokinin tunggal yaitu : MS + Benzil Adenin (BA) 0,5; 1,0; 1,5 dan 2,0 mg/l; tahap 2) respon tunas pada media kombinasi sitokinin dan auksin, yaitu : MS + BA 0,5 mg/l + NAA 0,1 mg/l; MS + BA 0,5 mg/l + NAA 0,5 mg/l; MS + BA 1,0 mg/l + NAA 0,1 mg/l dan MS + BA 1,0 mg/l + NAA 0,5 mg/l. Tahap 3) Daya multiplikasi dan penampilan tunas setelah subkultur pada media yang sama. Masingmasing percobaan disusun dengan rancangan acak lengkap, dan terdiri atas 5 ulangan. Parameter pengamatan meliputi jumlah tunas, tinggi tunas dan jumlah ruas serta penampilan visual. Hasil penelitian menunjukkan bahwa pada tahap pertama, penggunaan media MS + BA 0,5 menghasilkan jumlah tunas yang paling banyak dibandingkan yang lain, tetapi tidak berbeda nyata pada jumlah ruas dan tinggi eksplan. Sementara pada tahap dua, jumlah tunas terbaik didapat pada media dengan auksin rendah baik pada kombinasi sitokinin rendah dan tinggi. Sementara untuk jumlah ruas media terbaik adalah media dengan konsentrasi BA tinggi yang dikombinasi dengan NAA. Untuk tinggi tunas, media terbaik adalah MS + BA 1,0 mg/l + NAA 0,5 mg/l, tapi ditemukan eksplan yang menguning. Pada tahap ketiga, dari hasil subkultur kembali terlihat bahwa tunas yang bersumber dari pucuk pertumbuhannya baik sementara tunas yang berasal dari ruas ke-2 dan 3 sebagian menguning. Kata kunci : Ficus deltoidea Jack, tunas, induksi, in vitro, BA, NAAABSTRACT In vitro shoot induction of Mistleteo fig (Ficus deltoidea Jack) in Murashige & Skoog (MS) media with addition of BA and NAA Mistleteo fig (Ficus deltoidea) is one of endangered medicinal plants and used for female aphrodisiac. In vitro multiplication of the plant was done to find a number of shoots. This experiment was conducted in tissue culture laboratory of Germplasm and Breeding of IMACRI from January to December 2007, and aimed to find best media for shoot multiplication. This experiment was carried out in three steps: step 1) shoot respon in multiplication media using single cytokinin : MS + BA (0.5; 1.0; 1.5 and 2 mg/l); step 2) shoot respon in multiplication media of combined cytokinin and auxin : MS + BA 0.5 mg/l + NAA 0.1mg/l; MS + BA 0.5 mg/l + NAA 0.5 mg/l; MS + BA 1.0 mg/l + NAA 0.1 mg/l and MS + BA 1.0 + NAA 0.5 mg/l; and step 3) viability and visualization of the shoots after subcultured in the same media. The experiment was arranged using completely randomized design with 5 replicates. The parameters observed were of shoots and nodes, shoot height and performance. The results in the first step showed that MS + BA 0.5 mg/l media resulted in the highest number of shoots, but they were not significantly different in the number of nodes and shoots height. In the second step, highest number of shoots was found using low concentration of auxin combined with low and high concentration of cytokinin. Best medium for number of nodes was MS with high concentration of BA combined with NAA. For shoot height, the best medium was MS + BA 0.1 mg/l + NAA 0.5 mg/l, but the shoots turned yellow. In the third step, after subcultured, the shoots originated from plant tips performed well, however, those taken from second and third inter nodes partially turned yellow. Key words : Ficus deltoidea Jack, shoot, induction, in vitro, BA, NAA

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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