scholarly journals Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽  
10.15575/1344 ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 97-109
Author(s):  
Lamro Purba ◽  
Erni Suminar ◽  
Denny Sobardini ◽  
Wieny Rizky ◽  
Syariful Mubarok
Keyword(s):  
Tissue Culture ◽  
Leaf Number ◽  
Shoot Induction ◽  
Seed Technology ◽  
Randomized Design ◽  
Rooting Media ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

scholarly journals Multiplikasi in vitro stroberi kultivar Tochiotome dengan penambahan jenis dan konsentrasi sitokinin untuk perbanyakan bibit

Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Fast Time ◽  
Endogenous Auxin ◽  
Seed Technology ◽  
Randomized Design ◽  
Process Plants ◽  
Number Of Leaves ◽  
Completely Randomized Design

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture

scholarly journals PENGARUH KONSENTRASI BAYCLIN PADA PENCUCIAN II DAN BAP PADA MEDIA MS TERHADAP PERTUMBUHAN EKSPLAN TANAMAN PISANG KLUTUK (Musa paradisiaca. L) SECARA IN VITRO

2019 ◽  
Vol 33 (2) ◽  
pp. 163-168
Author(s):  
Zulkifli Zulkifli ◽  
Putri Lukmana Sari
Keyword(s):  
Tissue Culture ◽  
Phase Ii ◽  
Root Length ◽  
Musa Paradisiaca ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Number Of Shoots

This research aims to examie the effect of bayclin concentration on washing II and BAP in MS media on eksplan growth of klutuk banana in vitro and was conducted at the Tissue Culture Laboratory Lempes Pekanbaru from April to August 2016. The research used a completely randomized design (CRD) with 3 factorials and 3 replications. The first factor was the concentration of washing eksplan bayclin on phase II which consisted of four levels: K0 (concentration of 0%), K1 (concentration of 10%), K2 (concentration of 20%), and K3 (concentration of 30%). The second factor was the concentration of BAP contained on media MS consisted of four levels: T0 (BAP concentration 0 mg/liter), T1 (BAP concentration of 0.1 mg/liter), T2 (BAP concentration of 1.0 mg/liter) and T3 (BAP concentration of 10.0 mg/liter). The parameters observed were percentage of explants contaminated, age appears buds, the number of shoots/explant, length of shoots/explant, number of roots/explant (root), root length/explant and percentage of explant life. The results showed that effect of concentration on the washing bayclin II and BAP in MS Media on growth eksplan of klutuk banana in-vitro was the best treatment K2T2 with 33.33% of explants contaminated, age of appears buds 2,67 weeks after planting, the number of shoots 2,67/explant, length of shoots 5,50 cm/explant, number of roots 2.67/explant, root length 12,08 cm/explant and 58.33% of explants life.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals Plantlets Regeneration from Crown Bud Slicing of Pineapple (Ananas comosus)

2018 ◽  
Vol 10 (3) ◽  
pp. 484-490 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Neliyati Neliyati ◽  
Eliyanti Eliyanti
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Culture Technique ◽  
Leaf Number ◽  
Ananas Comosus ◽  
Culture Techniques ◽  
Lateral Shoots ◽  
Four Levels ◽  
Tissue Culture Techniques

Pineapple propagation by lateral shoots, suckers or crowns is often confronted with limited number of regenerated seedlings and high diversity in flowering and fruit formation. In order to solve this problem, this study offer an alternative method by using tissue culture techniques. This study aimed to determine the effect of growth regulators on plantlet regeneration from bud slicing of pineapple cv. Tangkit. Four levels of 2.4-D (0.0, 0.001, 0.01 and 0.1 ppm) in combination with BA (0.0, 0.1, 1.0 and 10.0 ppm) were tested on solid MS medium. Cultures were incubated in total darkness for a week followed by transfer to 16-hour photoperiod. Results showed that explants treated with 2,4-D and/or BA succeeded in regenerating adventitious shoots. Average leaf number did not differ significantly among treatments (P = 0.60). Highest leaf number (2.99 ± 0.23) was obtained on medium with 0.01 ppm 2,4-D without BA, followed by 0.1 ppm 2,4-D without BA (2.85 ± 0.33). Meanwhile, roots were only formed on medium with 0.1 ppm 2.4-D without BA (4.2 ± 0.37 per shoot). Thus, complete plantlets were regenerated only on medium supplemented with 0.1 ppm 2,4-D without BA. The growth of plantlets was relatively uniform, and plantlet acclimatization succeeded 100% on Jiffy pots. The finding of optimum concentration of 2.4-D and BA in this study is important to develop standard protocol for in vitro propagation of pineapple cv. Tangkit. Thus, the benefit of producing seeds in large quantities and relatively uniform in growth is made possible through tissue culture technique.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals In Vitro Screening Ketahanan Galur Padi (Oryza Sativa) B7 Hasil Rakitan Politeknik Negeri Lampung Terhadap Keracunan Besi (Fe)

2019 ◽  
Vol 19 (3) ◽  
pp. 241
Author(s):  
Onny Chrisna Pandu Pradana ◽  
Siti Novridha Andini
Keyword(s):  
Tissue Culture ◽  
Oryza Sativa ◽  
Iron Toxicity ◽  
In Vitro Screening ◽  
Culture Bottle ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
And Control

This research aimed to invstigate the response of paddy culture (B7 strain) assembled by Lampung State Polytechnic to the iron toxicity tolerance. The research was done at Plant Tissue Culture Laboratory, Lampung State Polytechnic, from July to September 2019. Treatments were single arranged in a completely randomized design with three replications. The treatment tried was five levels of Fe concentrations (5,6 ppm 28 ppm, 56 ppm, 84 ppm, 112 ppm, and control). Each replication consisted of three culture bottle containing one explant. The homogenity of data was tested using Barlett test. If the assumption were fulfilled then analysis of variance is executed using STATISTIX 10, and then followed by the Honest Significant Difference (HSD) test in 5% alpha for mean separation and RPA analysis. The result of this research showed that the B7 strain has tolerance to iron toxicity until 56 ppm of Fe concentration, it can be concluded from the PAR value of its strain (>0,50). Meanwhile in  84 and 112 ppm of Fe concentration, the RPA value of B7 starin (<0,50), and it is indicate that its strain is sensitive. 

RESPONSE OF TURMERIC SHOOT EXPLANT AFTER CYTOKININ WITH IN VITRO

2019 ◽  
Vol 24 (2) ◽  
pp. 143-152
Author(s):  
Marhan Nurullia ◽  
Erni Suminar Suminar ◽  
Anne Nurani
Keyword(s):  
Tissue Culture ◽  
Root Length ◽  
Air Flow ◽  
T Test ◽  
Shoot Height ◽  
Best Response ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Laminar Air Flow

This study was aimed at determining the response of turmeric shoot explants after the provision of various types and concentrations of cytokinins in vitro. This experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Padjadjaran University from January to April 2018. The data were analyzed using T-Test. The experimental method used in this research was Completely Randomized Design (CRD). Explant planting was carried out in Laminar Air Flow. The experiment consisted of 7 treatments consisting of 4 replications and each test consisted of 4 units. Observation of this experiment was carried out for 12 MST. The main observations were made on the data that were tested statistically namely the percentage of explant growing shoots, percentage of explant growing roots, shoot height, number of tuns, number of roots and root length. The treatments consisted of Control, 2.5 mg L-1 BAP, 5 mg L-1 BAP, 0.5 mg L-1 TDZ, 1 mg L-1 TDZ, 0.01 mg L-1 Zeatin and 0.1 mg L Zeatin -1. The results show that the treatment of 1 mg L-1 TDZ shows the best response to the growth of turmeric explants by increasing the number of turmeric shoot explants than the others.RESPONS EKSPLAN TUNAS KUNYIT SETELAH SITOKININ SECARA IN VITROTujuan dari penelitian ini yaitu untuk melihat respons eksplan tunas kunyit terhadap pemberian berbagai jenis dan konsentrasi sitokinin secara in vitro. Percobaan ini dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Padjadjaran dari bulan Januari sampai April 2018. Hasil percobaan dianalisis dengan Sample T-Test. Metode percobaan yang digunakan dalam penelitian ini yaitu Rancangan Acak Lengkap (RAL). Penanaman eksplan dilakukan di dalam Laminar Air Flow. Percobaan terdiri dari 7 perlakuan sebanyak 4 ulangan dan setiap ulangan terdiri dari 4 unit. Pengamatan percobaan ini dilakukan selama 12 MST. Pengamatan utama dilakukan terhadap data-data yang diuji secara statistik yakni persentase eksplan tumbuh tunas, persentse eksplan tumbuh akar, tinggi tunas, jumlah tunas, jumlah akar dan panjang akar. Perlakuan terdiri dari Kontrol; 2,5 mg L-1 BAP; 5 mg L-1 BAP; 0,5 mg L-1 TDZ; 1 mg L-1 TDZ; 0,01 mg L-1 Zeatin; dan 0,1 mg L-1 Zeatin. Hasil penelitian menunjukkan bahwa perlakuan 1 mg L-1 TDZ menunjukkan respons yang lebih baik terhadap pertumbuhan eksplan kunyit dengan meningkatkan jumlah tunas eksplan tanaman kunyit daripada yang lainnya.

PENGARUH PENAMBAHAN EKSTRAK JAGUNG MANIS DAN AIR KELAPA PADA MEDIA MS SECARA IN VITRO TERHADAP INDUKSI TUNAS ADVENTIF BAWANG PUTIH (Allium sativum L.)

2021 ◽  
Vol 23 (2) ◽  
pp. 92-97
Author(s):  
Marisa Gracia Bakara ◽  
Makhziah Makhziah ◽  
Guniarti Guniarti
Keyword(s):  
Allium Sativum ◽  
Sweet Corn ◽  
Water Concentration ◽  
Coconut Water ◽  
Time Treatment ◽  
Concentration Time ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels

[SWEET CORN EXTRACT AND COCONUT WATER EFFECT TO INDUCE GARLIC (Allium sativum L.) ADVENTIST SHOOTS BY IN-VITRO CULTURE]. Garlic (Allium sativum L.) is one of the horticultural plants used daily for various uses. This study aims to determine the best extract concentration of sweet corn and coconut water and its interaction for induction garlic Adventist shoots (Allium sativum L.) var. Lumbu Hijau. The study was conducted at the Biotechnology Laboratory, Agriculture Faculty, UPN ”Veteran” East Java, in February – April 2021. The experimental design used was a factorial completely randomized design (CRD). The first factor is four levels of extract of sweet corn concentration (0, 50, 100, and 150 g/L), while the second factor is four levels of coconut water concentration (0, 50, 100, and 150 mL/L). The results showed that the extract of sweet corn concentration-time treatment increased the growing adventist shoots percentage, adventist shoots number, and adventist shoots size. The section of sweet corn concentration of 150 g/L increased the rate of growing adventist shoots, adventist shoots number, and adventist shoots size. The treatment of coconut water concentration and its interaction with the extract of sweet corn concentration is still not able for induction garlic adventist shoots (Allium sativum L.) of the var. Lumbu Hijau.

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