Induksi Kalus dan Regenerasi Tiga Genotipe Tomat  (Solanum lycopersicon L.) melalui Kultur Antera

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del> genotype<ins cite="mailto:Windows%207" datetime="2016-10-31T15:07">s</ins>. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% while in Tora was 2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:07"></ins>% and Ratna was <del cite="mailto:Windows%207" datetime="2016-10-31T15:07"></del>0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%.Keywords: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah <del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>tomat varietas <ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>% lebih tinggi dibandingkan Tora (2.1<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:08"></ins>%) dan Ratna (<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.<del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:08"></del>8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4<del cite="mailto:Windows%207" datetime="2016-10-31T15:09"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:09"></ins>%).Kata kunci: <del cite="mailto:Windows%207" datetime="2016-10-25T11:47"></del><ins cite="mailto:Windows%207" datetime="2016-10-25T11:47"></ins>androgenesis, auksin, in vitro, media, sitokinin, tomat

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Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i2.13479 ◽

2016 ◽

Vol 44 (2) ◽

pp. 133

Author(s):

Cucu Gunarsih ◽

Bambang Sapta Purwoko ◽

Iswari Saraswati Dewi ◽

Dan Muhamad Syukur

Keyword(s):

Plant Regeneration ◽

Anther Culture ◽

Doubled Haploid ◽

Callus Induction ◽

Rainfed Rice ◽

Randomized Design ◽

Doubled Haploid Plants ◽

Completely Randomized Design ◽

N6 Medium ◽

Haploid Plants

ABSTRACT The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing: INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM putresin, and media for rooting was based on MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%, respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants), G5 (21 plants), and G6 (26 plants). Keywords: Callus induction, doubled haploid, rice

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Pertumbuhan dan Perkembangan Jaringan Meristem Bawang Merah (Allium ascalonicum L.) Kultivar Katumi Secara In Vitro

Jurnal Agro ◽

10.15575/1344 ◽

2017 ◽

Vol 4 (2) ◽

pp. 97-109

Author(s):

Lamro Purba ◽

Erni Suminar ◽

Denny Sobardini ◽

Wieny Rizky ◽

Syariful Mubarok

Keyword(s):

Tissue Culture ◽

Leaf Number ◽

Shoot Induction ◽

Seed Technology ◽

Randomized Design ◽

Rooting Media ◽

Completely Randomized Design ◽

Four Levels ◽

Induction Stage

This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS0media and also knowing the largest number of roots in rooting media for shallot by in vitro. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS0 media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L-1 kinetin without NAA,while the treatment of 2 mg L-1 kinetin with 0.01 mg L-1 NAA gave a better interaction for theshoot addition variable.

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Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical

Jurnal Hortikultura ◽

10.21082/jhort.v21n4.2011.p293-305 ◽

2011 ◽

Vol 21 (4) ◽

pp. 293 ◽

Cited By ~ 1

Author(s):

Budi Winarto

Keyword(s):

Anther Culture ◽

Callus Induction ◽

Culture Medium ◽

Callus Formation ◽

Potential Growth ◽

Randomized Design ◽

Ornamental Crops ◽

Completely Randomized Design ◽

Anthurium Andraeanum ◽

Positive Effect

Kultur anter merupakan salah satu teknologi haploid penting dalam produksi tanaman haploid ganda dan berhasil diaplikasikan pada berbagai jenis tanaman, namun aplikasi pada Anthurium belum pernah dilaporkan. Penelitian dan pengembangan kultur anter Anthurium yang difokuskan untuk mempelajari pengaruh glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias dari bulan Januari sampai dengan September 2008. Tujuan penelitian ialah mengetahui pengaruh kombinasi konsentrasi glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus pada kultur anter Anthurium. Spadik Anthurium andraeanum cv. Tropical, kalus hasil kultur anter serta medium Winarto dan Teixeira digunakan dalam studi ini. Glutamin dan serin pada konsentrasi 0, 250, 500, dan 750 mg/l diuji dalam percobaan ini. Percobaan disusun menggunakan rancangan acak lengkap pola faktorial dengan empat ulangan. Hasil studi menunjukkan bahwa penambahan glutamin dan serin pada medium terseleksi belum memberikan pengaruh yang signifikan terhadap induksi, pertumbuhan, dan regenerasi kalus. Glutamin pada konsentrasi 250 mg/l menginduksi potensi tumbuh anter hingga 48% dengan 21% anter beregenerasi dan 1,3 anter per perlakuan membentuk kalus. Sementara serin pada 500 mg/l merupakan konsentrasi yang paling potensial dalam induksi kalus dengan 55% potensi tumbuh anter, 24% anter beregenerasi, dan 1,4 anter per perlakuan membentuk kalus. Glutamin 250 mg/l merupakan konsentrasi terbaik dibanding konsentrasi yang lain dalam mendukung pertumbuhan dan regenerasi kalus. Perlakuan tersebut tanpa serin mampu menginduksi potensi pertumbuhan kalus hingga 77% dengan volume kalus mencapai 237 mm3 dan empat tunas dihasilkan per eksplan. Sementara perlakuan serin justru mereduksi pertumbuhan dan regenerasi kalus dan menstimulasi senesensi kalus yang berdampak pada pencoklatan dan kematiannya. Dari hasil penelitian ini dapat disarankan penggunaan glutamin dibanding serin dalam meningkatkan keberhasilan kultur anter Anthurium. Anther culture is one of important haploid technologies in producing double haploid lines and successfully applied in many plants, while the application in Anthurium is not reported yet. Research and development in anther culture of Anthurium focusing on studying the effect of glutamine and serine on callus induction, growth, and its regeneration was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute from January untill September 2008. Objective of this study was to know the effect of glutamine and serine on callus induction, growth, and its regeneration in anther culture of Anthurium. Spadix of Anthurium andraeanum cv. Tropical, callus derived from anther and Winarto and Teixeira medium were utilized in the study. Glutamine and serine of 0, 250, 500, and 750 mg/l were tested in the experiments. Factorial experiment was arranged by completely randomized design with four replications. Results of the study indicate that addition of glutamine and serine in selected culture medium gave moderate significant effect on induction, growth, and regeneration of callus. Glutamine in 250 mg/l induced potential growth of anther up to 48% with 21% regenerated anthers and 1.3 anthers per treatment producing calli, while 500 mg/l of serine was better concentration in callus formation with 55% potential growth of callus, 24% regenerated anthers and 1.4 anthers per treatment producing calli. In growth and regeneration of callus, supplementation of serine reduced callus capacity in growth and production of shoots and stimulated callus senescence causing browning and death of it, while 250 mg/l glutamine exhibited positive effect on them. The treatment without serine was able to induce potential growth of callus up to 77% with 237 mm3 per callus and four shoots produced per explants. Results of the study suggest application of glutamine rather than serine in improving anther culture of Anthurium.

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Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung

Jurnal AgroBiogen ◽

10.21082/jbio.v8n3.2012.p89-96 ◽

2016 ◽

Vol 8 (3) ◽

pp. 89

Author(s):

Iswari S Dewi ◽

Anggi Nindita ◽

Bambang S. Purwoko ◽

Darda Efendi

Keyword(s):

Growth Regulator ◽

Woody Plants ◽

De Novo ◽

Culture Media ◽

Shoot Induction ◽

Indirect Organogenesis ◽

Randomized Design ◽

Callus Initiation ◽

Completely Randomized Design

Propagation through tissue culture of plant species with rich secondary metabolites such as Jatropha curcas L. is difficult to obtain. However, once established, it can be used as one of the alternatives to supply uniform propagules. The effects of auxin and cytokinin on the regulation of de novo woody plants shoot development have been studied through shoot induction, differentiation and development. The objective of this research was to identify explant and suitable culture media for in vitro shoot induction through indirect organogenesis. Factorial experiment was arranged in a completely randomized design, replicated 20 times. The first factor was explants, i.e. cotyledons and hypocotyls. The second factor was MS media containing combination of plant growth regulator IAA (0, 0.05, and 0.1 mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of the experiment showed that the fastest callus initiation was achieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explants were cultured. Shoots with leaves can be induced from both cotyledons and hypocotyls. However, hypocotyls gave more shoots and leaves than cotyledons when cultured in MS + IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotyls and cotyledons were successfully rooted in MS medium without any growth regulator.

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PENGGUNAAN 2,4-D UNTUK INDUKSI KALUS KLON KAKAO UNGGUL SULAWESI 1

J-PEN Borneo : Jurnal Ilmu Pertanian ◽

10.35334/jpen.v2i3.1532 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

Yulianti Rasud ◽

Moh. Habil ◽

Tony Tony

Keyword(s):

Callus Induction ◽

Callus Formation ◽

Culture Techniques ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Completely Randomized Design ◽

Difference Test ◽

Honestly Significant Difference Test

ABSTRACT The multiplication of cocoa clones in conventional Sulawesi has not yet been able to fulfill the demand for large quantities of seeds because it is limited by the number of shoots and branches ready to be tapped, connected and oculated and takes longer to produce large quantities of seeds. One alternative in overcoming this problem is plant proragation using tussue culture techniques. The aim of this experiment was to determine the appropriate of 2,4-D for callus induction of superior cocoa clones Sulawesi via in vitro culture. This experiment used Completely Randomized Design with five treatments, namely 0.50 ppm 2,4-D, 0.75 ppm 2,4-D, 1.00 ppm 2,4-D, 1.25 ppm 2,4-D and 1.50 ppm 2,4-D. Parameters observed consisted of the time, percentage, color and texture of calli. Data was analized by using analysis of variance and differences between mean treatments were determined by Honestly Significant Difference Test at 5% level. Results of this experiment indicated that the ability of different callus induction at various concentrations of 2,4-D for superior cocoa clones in Sulawesi 1 was tried. it was obtained the quickest callus formation at concentration 0.50ppm 2,4-D namely average 4.22 WAC with the percentage of callus formation was up to 99,33%. Keywords: Callus Induction, Clones Sulawesi 1, 2,4-D ABSTRAK Perbanyakan klon kakao Sulawesi secara konvensional saat ini belum dapat memenuhi permintaan bibit dalam jumlah besar karena sangat dibatasi oleh jumlah tunas dan cabang yang siap disetek, disambung, dan diokulasi serta dibutuhkan waktu yang lebih lama untuk menghasilkan bibit dalam jumlah besar. Salah satu alternatif dalam mengatasi masalah tersebut adalah perbanyakan tanaman dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk memperoleh protokol yang tepat dalam menginduksi kalus sebagai upaya awal dalam perbanyakan tanaman kakao melalui embryogenesis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 level perlakuan yaitu 0,50 ppm 2,4-D, 0,75 ppm 2,4-D, 1,00 ppm 2,4-D, 1,25 ppm 2,4-D dan 1,50 ppm 2,4-D. Pengamatan dilakukan terhadap saat muncul kalus, persentase eksplan berkalus, warna kalus dan tekstur kalus. Data diolah dengan analisis ragam dan perbedaan antar perlakuan ditentukan dengan Uji Beda Nyata Jujur pada taraf 5%. Hasil penelitian menunjukkan kemampuan induksi kalus berbeda pada berbagai konsentrasi 2,4-D untuk klon kakao unggul Sulawesi 1 yang dicobakan. Saat muncul kalus paling cepat diperoleh pada konsentrasi 0,5 ppm 2,4-D yaitu rata-rata 16,67 HST dengan persentase pembentukan kalus tertinggi mencapai 99,33%. Selanjutnya, warna dan tekstur kalus yang dihasilkan yaitu remah putih dan remah kecoklatan. Kata Kunci: Induksi Kalus, Klon Sulawesi 1, 2,4-D.

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Callus Induction on True Shallot Seed Explant Using a Combination of BA and 2,4-D

Ilmu Pertanian (Agricultural Science) ◽

10.22146/ipas.26276 ◽

2018 ◽

Vol 2 (3) ◽

pp. 137 ◽

Cited By ~ 3

Author(s):

Krisna Dharmayanti ◽

Endang Sulistyaningsih ◽

Rani Agustina Wulandari

Keyword(s):

Callus Induction ◽

In Vitro Selection ◽

Callus Formation ◽

Optimum Combination ◽

Shoot Height ◽

Randomized Design ◽

Allium Cepa L ◽

Callus Proliferation ◽

Completely Randomized Design

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0–0, 2–1, 2–2, 2–3, and 2–4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1– 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.

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Induksi Kalus Tanaman Kentang Dombu (Solanum tuberosum L.) Secara In Vitro Dengan Pemberian ZPT 2,4-D (Dichlorophenoxy Acetid Acid)

Natural Science Journal of Science and Technology ◽

10.22487/25411969.2019.v8.i2.13538 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Siti RLR Idris ◽

Asri Pirade Paserang

Keyword(s):

Solanum Tuberosum ◽

Callus Induction ◽

Culture Media ◽

Callus Formation ◽

Solanum Tuberosum L ◽

Randomized Design ◽

Completely Randomized Design

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass

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INDUKSI KALUS CENGKEH DARI EKSPAN DAUN MENGGUNAKAN 2,4-D SECARA IN VITRO

J-PEN Borneo : Jurnal Ilmu Pertanian ◽

10.35334/jpen.v2i3.1533 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

Yulianti Rasud ◽

Zainuddin Basri ◽

Nirwan Sahiri

Keyword(s):

Growth Regulators ◽

Callus Induction ◽

Callus Formation ◽

Cell Mass ◽

Medium Composition ◽

Ms Medium ◽

Plant Parts ◽

Randomized Design ◽

Completely Randomized Design

ABSTRACT Callus induction is one method of tissue culture which is done by stimulating cell division continuously from certain plant parts such as leaves, roots, stems, and so on by using growth regulators to form cell mass. The cell mass (callus) will then regenerate through organogenesis or embryogenesis to become a new plant. One of the growth regulators used for callus induction is 2,4-D. The aims of this experiments was to evaluate the best concentration of 2,4-D for callus induction of clove leaves. The experiment used Completely Randomized Design with treatment tested was concentrations of 2,4-D, consisted of six levels, namely 0.5 ppm, 1.0 ppm, 1.5 ppm, 2.0 ppm, 2.5 ppm and 3.0 ppm. Results of this experiments indicated that the best medium composition for callus induction was MS medium supplemented with 0.5 ppm 2,4-D. In the medium composition, the fastest callus formation, namely 6.00 weeks after culture and the percentage of callus formation reached 100% with the color and texture of the resulting callus white and crumb. Keyword : Callus Induction, Clove, 2,4-DABSTRAK Induksi kalus merupakan salah satu metode kultur jaringan yang dilakukan dengan jalan memacu pembelahan sel secara terus menerus dari bagian tanaman tertentu seperti daun, akar, batang, dan sebagainya dengan menggunakan zat pengatur tumbuh hingga terbentuk massa sel. Massa sel (kalus) tersebut selanjutnya akan beregenerasi melalui organogenesis ataupun embriogenesis hingga menjadi tanaman baru. Salah satu zat pengatur tumbuh yang digunakan untuk induksi kalus adalah 2,4-D. Penelitian ini bertujuan menentukan konsentrasi 2,4-D yang lebih baik untuk induksi kalus daun cengkeh. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Media dasar yang digunakan adalah media MS yang ditambahkan berbagai konsentrasi 2,4-D yaitu 0,50 ppm, 1,5 ppm, 2 ppm, 2,5 ppm, dan 3 ppm. Hasil penelitian menunjukkan bahwa komposisi media yang terbaik untuk induksi kalus daun cengkeh adalah media MS yang ditambahkan 0,5 ppm 2,4-D. Pada komposisi media tersebut diperoleh saat muncul kalus paling cepat, yaitu rata-rata 6,00 MST dengan persentase pembentukan kalus tertinggi mencapai 100% dengan warna dan tekstur kalus yang dihasilkan putih dan remah. Kata Kunci : Induksi Kalus, Cengkeh, 2,4-D.

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Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v22i1.36007 ◽

2020 ◽

Vol 22 (1) ◽

pp. 39

Author(s):

Amalina Wahyuni ◽

Benni Satria ◽

Aprizal Zainal

Keyword(s):

Tissue Culture ◽

Callus Induction ◽

Economic Value ◽

Market Demand ◽

Compact Structure ◽

Randomized Design ◽

Aquilaria Malaccensis ◽

Completely Randomized Design ◽

Range Test

Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact. Yellow callus and compact structure could be utilized for organogenesis.

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A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP

BIOVALENTIA Biological Research Journal ◽

10.24233/biov.2.1.2016.32 ◽

2016 ◽

Vol 2 (1) ◽

Cited By ~ 1

Author(s):

Laily Ilman Widuri ◽

Parawita Dewanti ◽

Bambang Sugiharto

Keyword(s):

Somatic Embryogenesis ◽

Shoot Regeneration ◽

Callus Induction ◽

Saccharum Officinarum ◽

Randomized Design ◽

Indirect Somatic Embryogenesis ◽

Simple Protocol ◽

Callus Proliferation ◽

Completely Randomized Design

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing 3 mgL-1of 2,4-D.

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