scholarly journals Keefektifan Pelapisan Benih terhadap Peningkatan Mutu Benih Padi Selama Penyimpanan

2015 ◽  
Vol 34 (2) ◽  
pp. 145
Author(s):  
Ikrarwati Ikrarwati ◽  
Satriyas Ilyas ◽  
AmiyarsI Mustika Yukti
Keyword(s):  
Pathogenic Bacteria ◽  
Seed Viability ◽  
Seed Coating ◽  
Rice Seed ◽  
Clove Oil ◽  
Arabic Gum ◽  
Control Seed ◽  
Citronella Oil ◽  
Lemon Grass ◽  
Four Levels

Seed coating using biological pesticides such as clove oil and citronella oil, was expected to have the ability to control seed-borne pathogens on rice. The aim of the research was to determine the effectiveness of seed coating using clove oil and lemon grass oil against seed-borne fungi and bacteria on rice seed variety “Hipa 8” during storage. The experiment was conducted in Cimanggis, from February to September 2012, using completely randomized design with single factor, consisting of four levels: (1) clove oil 1% + chitosan 3%; (2) lemon grass oil 2% + carboxymethyl-cellulose 1%; (3) Synthetic pesticide (streptomycin sulphate 0.04% + benomyl 0.1%) + arabic gum 10%; and (4) control (without coating). Results showed that seedborne fungi pathogens on seed of Hipa 8 rice were detected at 6-month storage consisted of Fusarium sp., Curvularia sp., Alternaria sp., Cladosporium sp., Aspergillus sp. and Penicillium sp. The detected pathogenic bacteria were Xanthomonas oryzae pv. oryzae and X. campestris pv. oryzicola. Coating formula most compatible with the rice seed of HIPA 8 was chemical pesticide + 10% arabic gum, which suppressed fungal infection from 80% to 45% at the first month and from 90% to 70% at the fifth month, suppressed populations of Xoo + Xco from 7.6 x 108 cfu/g of seed to 5.86 x 105 cfu/g of seed at the first month and 7.0 x 106 cfu/g of seed to 1.4 x 104 cfu/g of seeds at the sixth month. The treatment caused the smallest decrease of seed viability compared to the other coating treatments.

scholarly journals Aplikasi Teknologi Seed Coated dan Seed Delinted pada Benih Kapas (Gossypium hirsutum L.)

2019 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Taufiq Hidayat RS ◽  
Nurindah Nurindah ◽  
Anik Herawati
Keyword(s):  
Gossypium Hirsutum ◽  
Seed Quality ◽  
Block Design ◽  
Seed Viability ◽  
Environmentally Friendly ◽  
Maximum Growth ◽  
Seed Coating ◽  
Seed Treatments ◽  
Gossypium Hirsutum L ◽  
Arabic Gum

<p><em>Seed coated</em> merupakan teknologi pelapisan benih dengan bahan tertentu untuk mempertahankan mutu benih dan membuat bentuk benih lebih teratur. Prosesing benih kapas saat ini masih menggunakan bahan kimia seperti asam sulfat (<em>seed delinted</em>) untuk menghilangkan kabu-kabu (<em>linter</em>) yang masih menempel pada biji setelah proses pemisahan serat dan biji. <em>Seed delinted</em> memungkinkan terjadinya kerusakan kulit hingga lembaga biji dan dapat menimbulkan masalah lingkungan dari limbah yang dihasilkan dalam proses tersebut. Penelitian ini bertujuan untuk menguji pengaruh perlakuan benih pada tiga jenis varietas terhadap viabilitas benih kapas. Penelitian ini menggunakan Rancangan Faktorial dalam RAK. Faktor Pertama terdiri atas beberapa perlakuan benih yaitu benih berkabu, benih <em>delinted</em>,<em> coated</em> dengan tapioka dan kaolin serta <em>coated </em>dengan<em> arabic gum</em>. Sedangkan, faktor kedua terdiri atas varietas kapas yaitu Kanesia 10, Kanesia 18 dan Kanesia 19. Hasil penelitian menunjukkan bahwa interaksi antara perlakuan benih dengan varietas kapas berpengaruh nyata untuk parameter panjang radikula dan menghasilkan koefesien keragaman mencapai 9.85%. Perlakuan benih <em>coated</em> dengan bahan <em>arabic gum</em> menunjukkan hasil yang terbaik untuk semua parameter pengamatan. Sedangkan, varietas Kanesia 10 menunjukkan persentase keserempakan tumbuh, persentase daya berkecambah, dan persentase potensi tumbuh maksimum terbaik masing-masing 92.25%, 96.25%, dan 98.00%. Perlakuan benih dengan teknologi <em>seed coated</em> menggunakan <em>arabic gum</em> pada Varietas Kanesia 10 dapat menunjukkan persentase viabilitas benih kapas terbaik.</p><p> </p><p><strong>Effect of Seed Coating on the Seeds Viability of Three Cotton Varieties ( Gossypium hirsutum L.) </strong></p><p><br />Seed coating technology with certain materials is objected to maintain seed quality and to make seed shapes more regular. Currently, cotton seeds processing is using chemicals such as sulfuric acid (acid seed delinted) to remove the linter which is still attached to the seeds after the separation of fibers and seeds. Acid seed delinting could causing damage on the seed skin as well as to the seed embryo and also cause environmental problems from the waste produced in the process. Seed coated technology has the prospect to be applied in the process of cotton seeding, so the process becomes environmentally friendly. This study aims to evaluate the effect of seed coating treatment on three cotton varieties on the seed viability. This study uses Randomized Block Design Factorial. The first factor consisted of four seed treatments namely fuzzy seed (control), seed delinted, seed coated with tapioca and kaolin and seed coated with arabic gum. The second factor were cotton varieties namely Kanesia 10, Kanesia 18, and Kanesia 19. The results showed that the interaction between seed treatments with cotton varieties significantly affected the radicular length parameters and produced a coefficient of varians 9.85%. Seed coated with arabic gum showed the best results for all observation parameters. Kanesia 10 showed the best of growing simultaneity, germination, and the potential maximum growth by 92%, 96%, and 98%, recpectively. The cotton cotton seed coated with arabic gum is prospective to be applied in the cotton seeding process as an alternative to the acid delinting technique that is not environmentally friendly. </p>

scholarly journals EFFECT OF THE TREATMENT OF RICE SEED INFECTED BY XANTHOMONAS ORYZAE PV. ORYZAE ON GROWTH AND YIELD OF RICE IN THE FIELD

2017 ◽  
Vol 2 (2) ◽  
pp. 54-62
Author(s):  
Ahmad Zamzami ◽  
Satriyas Ilyas
Keyword(s):  
Seed Treatment ◽  
Seed Viability ◽  
Dry Weight ◽  
Biological Agents ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Growth And Yield ◽  
Rice Seed ◽  
Citronella Oil ◽  
Main Plot

This research aimed to study the effect of treatment of rice seed infected with X. oryzae pv. Oryzae naturally to control bacterial leaf blight and to increase growth and yield of rice in the field. The research used Slit plot design with the main plot of variety consisting of IR64 and Ciherang, while the subplot is a seed treatment consisting of control, bactericide 0.2%  (Agrept 20WP), 1% citronella oil, biological agent Pseudomonas diminuta (McFarland IV scale ), matriconditioning + Agrept 0.2%, matriconditioning + 1% citronella oil, and matriconditioning + P. diminuta. Although seed treatment has not been able to control the bacterial leaf blight, it can increase the growth of seed and yield. Treatment of matriconditioning + Agrept 0.2% can increase the seed viability and dry weight of seedlings. Height of seedlings can be increased by the treatment of citronella oil, biological agents P. diminuta, matriconditioning + agrept 0.2%, and  matriconditioning + 1% citronella oil. Treatment of citronella oil, matriconditioning + P. diminuta, biological agents P. diminuta, and matriconditioning + Agrept 0.2% can increase the  estimated yield of ubinan/CCE harvest

scholarly journals Efektivitas Seed Coating dan Biopriming dengan Rizobakteri dalam Mempertahankan Viabilitas Benih Cabai dan Rizobakteri selama Penyimpanan

2018 ◽  
Vol 8 (3) ◽  
pp. 192
Author(s):  
Ita Madyasari ◽  
Candra Budiman ◽  
Syamsuddin , ◽  
Dyah Manohara ◽  
Satriyas Ilyas
Keyword(s):  
Sodium Alginate ◽  
Seed Treatment ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Vigor ◽  
Hot Pepper ◽  
Seed Coating ◽  
Control Seed ◽  
Randomized Design ◽  
Two Factors

<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>The objective of the study was to obtain the best coating formula for hot pepper seeds, and evaluate the effect of seed coating and biopriming with rhizobacteria on viability of hot pepper seeds and rhizobacteria during storage. </em><em>Experiment 1 was arranged in a completely randomized design with one factor i.e. 11 coating formula. Experiment 2 was arranged in a nested plot design with two factors, storage period (0, 4, 8, 12, 16, 20, and 24 weeks) as main factor and seed treatment consisted of 11 treatments (control, seed coating with E1+F2B1, ST116B, CM8; biopriming 24 h with E1+F2B1, ST116B, CM8; biopriming 48 h with E1+F2B1, ST116B, and CM8; priming metalaxyl) as nested factor. Result of experiment 1 indicated that the</em><em> best coating formula for hot pepper seed was sodium alginate 2.5% and was used in experiment 2. </em><em>Experiment 2 showed that seed coating and biopriming with rhizobacteria were able to maintain seed viability (79-89%) for 24 weeks of storage at 27-30 <sup>0</sup>C as compared to priming metalaxyl (54%). Biopriming E1+F2B1 24 h or CM8 48 h resulted in the highest index of seed vigor after 24 weeks of storage. Population of rhizobacteria in seed tissue decreased in bioprimed seeds from 10<sup>5</sup>-10<sup>7</sup> cfu g<sup>-1</sup> to 10<sup>4 </sup>cfu g<sup>-1</sup> after being stored for 24 weeks. </em></p><p><em>Keywords: rhizobacteria isolates, seed treatment, seed vigor, sodium alginate</em></p><p><em> </em></p><p align="center"><strong>ABSTRAK <br /></strong></p><p>Penelitian ini bertujuan mendapatkan formula <em>coating</em> terbaik pada benih cabai dan mengevaluasi pengaruh <em>seed coating</em> dan <em>biopriming</em> dengan rizobakteri dalam mempertahankan viabilitas benih cabai dan rizobakteri selama penyimpanan. Percobaan 1 menggunakan rancangan acak lengkap satu faktor yang terdiri atas 11 formula <em>coating</em>. Percobaan 2 menggunakan rancangan petak tersarang dua faktor, periode simpan (0, 4, 8, 12, 16, 20, dan 24 minggu) sebagai faktor utama dan perlakuaan benih yang terdiri atas 11 perlakuan (kontrol, <em>seed coating </em>dengan E1+F2B1<em>, </em>ST116B<em>, </em>CM8<em>; biopriming </em>24 jam dengan E1+F2B1<em>, </em>ST116B, CM8; <em>biopriming </em>48 jam dengan E1+F2B1<em>, </em>ST116B, dan CM8<em>; priming </em>metalaksil)<em> </em>sebagai faktor tersarang<em>.</em> Hasil Percobaan 1 menunjukkan bahwa formula <em>coating</em> terbaik untuk benih cabai ialah natrium alginat 2.5% dan digunakan pada percobaan 2. Percobaan 2 menunjukkan bahwa <em>seed coating</em> dan <em>biopriming</em> dengan rizobakteri mampu mempertahankan viabilitas benih (78-89%) selama 24 minggu penyimpanan pada suhu 27-30 <sup>0</sup>C<em> </em>dibandingkan <em>priming</em> metalaksil (54%). <em>Biopriming</em> E1+F2B1 24 jam atau <em>biopriming</em> CM8 48 jam menghasilkan indeks vigor paling tinggi setelah disimpan selama 24 minggu. Populasi rizobakteri di dalam jaringan benih menurun pada benih yang diberi perlakuan <em>biopriming</em> dari 10<sup>5</sup>-10<sup>7</sup> cfu g<sup>-1</sup> menjadi 10<sup>4 </sup>cfu g<sup>-1</sup> setelah disimpan selama 24 minggu.</p><p>Kata kunci: isolat rizobakteri, natrium alginat, perlakuan benih, vigor</p>

scholarly journals Do applications of systemic herbicides when green fruit are present prevent seed production or viability of Alliaria petiolata (garlic mustard)?

2021 ◽  
pp. 1-17
Author(s):  
Leo Roth ◽  
José Luiz C. S. Dias ◽  
Christopher Evans ◽  
Kevin Rohling ◽  
Mark Renz
Keyword(s):  
Seed Production ◽  
Seed Viability ◽  
Early Spring ◽  
Alliaria Petiolata ◽  
Late Spring ◽  
Garlic Mustard ◽  
Viable Seed ◽  
Application Timing ◽  
Control Seed ◽  
Second Year

Garlic mustard [Alliaria petiolata (M. Bieb.) Cavara & Grande] is a biennial invasive plant commonly found in the northeastern and midwestern United States. Although it is not recommended to apply herbicides after flowering, land managers frequently desire to conduct management during this timing. We applied glyphosate and triclopyr (3% v/v and 1% v/v using 31.8% and 39.8% acid equivalent formulations, respectively) postemergence to established, second-year A. petiolata populations at three locations when petals were dehiscing, and evaluated control, seed production and seed viability. Postemergence glyphosate applications at this timing provided 100% control of A. petiolata by 4 weeks after treatment at all locations whereas triclopyr efficacy was variable, providing 38-62% control. Seed production was only reduced at one location, with similar results regardless of treatment. Percent seed viability was also reduced, and when combined with reductions in seed production, we found a 71-99% reduction in number of viable seed produced plant-1 regardless of treatment. While applications did not eliminate viable seed production, our findings indicate that glyphosate and triclopyr applied while petals were dehiscing is a viable alternative to cutting or hand-pulling at this timing as it substantially decreased viable A. petiolata seed production. Management Implications Postemergence glyphosate and triclopyr applications in the early spring to rosettes are standard treatments used to manage A. petiolata. However, weather and other priorities limit the window for management, forcing field practitioners to utilize more labor-intensive methods such as hand-pulling. It is not known how late in the development of A. petiolata these herbicides can be applied to prevent viable seed production. Since prevention of soil seedbank replenishment is a key management factor for effective long-term control of biennial invasive species, we hypothesized late spring foliar herbicide applications to second year A. petiolata plants when flower petals were dehiscing could be an effective management tool if seed production or viability is eliminated. Our study indicated that glyphosate applications at this timing provided 100% control of A. petiolata plants by 4 weeks after treatment at all locations, whereas triclopyr efficacy was inconsistent. Although both glyphosate and triclopyr decreased viable seed production to nearly zero at one of our three study locations, the same treatments produced significant amounts of viable seed at the other two locations. Our findings suggest late spring glyphosate and triclopyr applications should not be recommended over early spring applications to rosettes for A. petiolata management, as our late spring application timing did not prevent viable seed production, and may require multiple years of implementation to eradicate populations. Nonetheless, this application timing holds value in areas devoid of desirable understory vegetation compared to no management practices or mechanical management options including hand-pulling when fruit are present, as overall viable seed production was reduced to similar levels as these treatments.

scholarly journals DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm

2019 ◽  
Vol 116 (19) ◽  
pp. 9652-9657 ◽  
Author(s):  
M. Yvonne Kim ◽  
Akemi Ono ◽  
Stefan Scholten ◽  
Tetsu Kinoshita ◽  
Daniel Zilberman ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Vegetative Cell ◽  
Seed Viability ◽  
Dna Demethylation ◽  
Central Cell ◽  
Dna Glycosylase ◽  
Rice Seed ◽  
Vegetative Cells ◽  
Active Dna Demethylation ◽  
Cell Genome

Epigenetic reprogramming is required for proper regulation of gene expression in eukaryotic organisms. In Arabidopsis, active DNA demethylation is crucial for seed viability, pollen function, and successful reproduction. The DEMETER (DME) DNA glycosylase initiates localized DNA demethylation in vegetative and central cells, so-called companion cells that are adjacent to sperm and egg gametes, respectively. In rice, the central cell genome displays local DNA hypomethylation, suggesting that active DNA demethylation also occurs in rice; however, the enzyme responsible for this process is unknown. One candidate is the rice REPRESSOR OF SILENCING1a (ROS1a) gene, which is related to DME and is essential for rice seed viability and pollen function. Here, we report genome-wide analyses of DNA methylation in wild-type and ros1a mutant sperm and vegetative cells. We find that the rice vegetative cell genome is locally hypomethylated compared with sperm by a process that requires ROS1a activity. We show that many ROS1a target sequences in the vegetative cell are hypomethylated in the rice central cell, suggesting that ROS1a also demethylates the central cell genome. Similar to Arabidopsis, we show that sperm non-CG methylation is indirectly promoted by DNA demethylation in the vegetative cell. These results reveal that DNA glycosylase-mediated DNA demethylation processes are conserved in Arabidopsis and rice, plant species that diverged 150 million years ago. Finally, although global non-CG methylation levels of sperm and egg differ, the maternal and paternal embryo genomes show similar non-CG methylation levels, suggesting that rice gamete genomes undergo dynamic DNA methylation reprogramming after cell fusion.

Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Pan Wang ◽  
Dong Li ◽  
Li-jun Wang ◽  
Benu Adhikari
Keyword(s):  
High Temperature ◽  
Ambient Temperature ◽  
Seed Viability ◽  
Seed Vigor ◽  
Drying Rate ◽  
Rice Seed ◽  
Rice Cultivars ◽  
Moisture Contents ◽  
Rice Seeds ◽  
Tempering Process

AbstractThis work aimed at determining whether high temperature intermittent drying followed by tempering at ambient temperature could preserve the seed viability and vigor.JaponicaandIndicarice seeds with 21.2 % and 22.6 % wet moisture contents (w.b.) were dried at 50 ºC and 60ºC for either 5, 10, 15 or 20 minutes, followed by tempering at 25ºC for 45 minutes. Each drying cycle was repeated until the rice seeds were dried to 12.0 % (w.b.). The drying rate was improved and the total in-dryer time was reduced in the intermittent drying when compared to continuous drying, due to the tempering process. The seed vigor was significantly reduced by intermittent drying at 60 °C with all exposure times, although the seed still kept the ability to germinate for both rice cultivars. The intermittent drying at 50 ºC for 5 minutes per drying cycle preserved the seed vigor ofJaponicarice well, while inIndicarice, the intermittent drying at 50 ºC up to 20 minutes could still be able to maintain the seed vigor.

scholarly journals Elimination of Escherichia coli O157:H7 from Alfalfa Seeds through a Combination of High Hydrostatic Pressure and Mild Heat

2009 ◽  
Vol 75 (7) ◽  
pp. 1901-1907 ◽  
Author(s):  
Hudaa Neetoo ◽  
Thompson Pizzolato ◽  
Haiqiang Chen
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Elevated Temperatures ◽  
Treatment Time ◽  
Seed Viability ◽  
Treatment Temperature ◽  
Reduced Pressure ◽  
Unique Challenge ◽  
Produce Safety ◽  
Alfalfa Seeds

ABSTRACT Escherichia coli O157:H7 has been associated with contaminated seed sprout outbreaks. The majority of these outbreaks have been traced to sprout seeds contaminated with low levels of pathogens. Sanitizing sprout seeds presents a unique challenge in the arena of produce safety in that even a low residual pathogen population remaining on contaminated seed after treatments appears capable of growing to very high levels during sprouting. In this study, the effectiveness of high-pressure treatment in combination with low and elevated temperatures was assessed for its ability to eliminate E. coli O157:H7 on artificially contaminated alfalfa seeds. Inoculated seed samples were treated at 600 MPa for 2 min at 4, 20, 25, 30, 35, 40, 45, and 50°C. The pressure sensitivity of the pathogenic bacteria was strongly dependent on the treatment temperature. At 40°C, the process was adequate in eliminating a 5-log-unit population on the seeds with no adverse effect on seed viability. Three treatments carried out at reduced pressure levels and/or extended treatment time, 550 MPa for 2 min at 40°C, 300 MPa for 2 min at 50°C, and 400 MPa for 5 min at 45°C, were equally lethal to the pathogen. When all three treatments were compared in terms of their impact on seed viability, the process of 550 MPa for 2 min at 40°C was the most desirable, achieving final germination percentages and sprout sizes statistically similar to those of control untreated seeds (P > 0.05).

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