Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Pan Wang ◽  
Dong Li ◽  
Li-jun Wang ◽  
Benu Adhikari
Keyword(s):  
High Temperature ◽  
Ambient Temperature ◽  
Seed Viability ◽  
Seed Vigor ◽  
Drying Rate ◽  
Rice Seed ◽  
Rice Cultivars ◽  
Moisture Contents ◽  
Rice Seeds ◽  
Tempering Process

AbstractThis work aimed at determining whether high temperature intermittent drying followed by tempering at ambient temperature could preserve the seed viability and vigor.JaponicaandIndicarice seeds with 21.2 % and 22.6 % wet moisture contents (w.b.) were dried at 50 ºC and 60ºC for either 5, 10, 15 or 20 minutes, followed by tempering at 25ºC for 45 minutes. Each drying cycle was repeated until the rice seeds were dried to 12.0 % (w.b.). The drying rate was improved and the total in-dryer time was reduced in the intermittent drying when compared to continuous drying, due to the tempering process. The seed vigor was significantly reduced by intermittent drying at 60 °C with all exposure times, although the seed still kept the ability to germinate for both rice cultivars. The intermittent drying at 50 ºC for 5 minutes per drying cycle preserved the seed vigor ofJaponicarice well, while inIndicarice, the intermittent drying at 50 ºC up to 20 minutes could still be able to maintain the seed vigor.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Deteksi Cepat Viabilitas Benih Padi Menggunakan Gelombang Near Infrared dan Model Jaringan Saraf Tiruan

2014 ◽  
Vol 33 (2) ◽  
pp. 77
Author(s):  
Jonni Firdaus ◽  
Rokhani Hasbullah ◽  
Usman Ahmad ◽  
M. Rahmad Suhartanto
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Soluble Protein ◽  
Input Data ◽  
Near Infrared ◽  
Seed Viability ◽  
Accelerated Aging ◽  
Rice Seed ◽  
Ann Models ◽  
Rice Seeds

Viability is an important component of seed quality, which could be detained by germinating the seeds. Currently testing the seed viability of rice takes a long time (5-14 days), so it becomes a limiting factor in the seed production process. An alternative method for rapid seed viability detection is using the Near Infrared (NIR) spectra and using artificial neural network (ANN) as a data processing system. This research was aimed to study the use of NIR spectra and ANN to predict the viability of rice seeds. NIR reflectance (1,000-2,500 nm) of a Ciherang rice seed samples (40 grams), was used as the input data to develop the ANN model. A total of 60 samples were subjected to accelerated aging to obtain various levels of germination. The development of ANN models was done through calibration and validation of NIR spectra to the viability parameters. As ANN input, NIR reflectance of seed sample was given pretreatment data such as normalization, first derivative, second derivative, standard normal variate (SNV) and principal component analysis (PCA). The results showed that longer accelerated aging caused a decrease in seed viability. This was also indicated by the decrease in soluble protein and an increase in free fatty acids. The intensity of the NIR absorbance spectra also showed the same in the absorption region of soluble protein and free fatty acids. The best ANN models to predict the germination was 10PC-5-3 ANN with the SNV NIR reflectance used as the input data. Coefisien correlation of the validation was 0.8947, the value of ratio performance deviation was 2.2359 and the standard error performance was 9.9233%. The use of NIR spectra and ANN was potentially useful to perdict the viability of rice seeds more rapidly.

scholarly journals Keefektifan Asap Cair dan Elektroterapi untuk Mengeliminasi Infeksi Xanthomonas oryzae pv. oryzae pada Benih Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 54
Author(s):  
Rizky G S Purnama ◽  
Kikin Hamzah Mutaqin ◽  
Efi Toding Tondok
Keyword(s):  
Seed Germination ◽  
Bacterial Population ◽  
Seed Quality ◽  
Seed Vigor ◽  
Xanthomonas Oryzae ◽  
Rice Seed ◽  
Liquid Smoke ◽  
Rice Seeds ◽  
Time Required ◽  
Total Seed

The Effectiveness of Liquid Smoke and Electrotherapy to Eliminate Infection of Xanthomonas oryzae pv. oryzae in Rice SeedBacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is a seed-borne disease that can reduce the productivity of rice. Alternative treatments that have not been widely developed is application of liquid smoke and electrotherapy. The aim of this research was to study the effectiveness of liquid smoke and electrotherapy and their combinations to eliminate X. oryzae pv. oryzae without damaging seed quality. The study consisted of three experimental stages: (1) Liquid smoke treatment on the viability of the bacteria and rice seeds; (2) Electrotherapy treatment on the viability of bacteria and rice seeds; (3) Combination of liquid smoke and electrotherapy treatment in bacteria-infected rice seed. The results showed that liquid smoke concentration (5% for 30 minutes) was able to reduce 83.33% population of X. oryzae pv.oryzae on rice seeds with 94.33% seed vigor, 98.00% germination, and time required to achieve 50% of total seed emergence (T50) of 3.13 days. Electrotherapy treatment (400 mA for 20 minutes) was not only eliminated X. oryzae pv. oryzae on rice seeds up to 100% but also maintained seed vigor 81.67%, germination 89.33%, and T50 3.47 days. Electrotherapy treatment (400 mA for 20 minutes) followed by immersion of infected rice seeds in liquid smoke concentration (1% for 30 minutes) could reduce 94.59% bacteria population while maintaining 86.00% seed germination. Treatment of electrotherapy (400 mA for 20 minutes) followed by immersion seeds into liquid smoke concentration (5% for 30 minutes) could reduce bacterial population up to 100%, but caused phytotoxicity effect to the seedling.

scholarly journals Combined Effects of Biological and Chemical Treatment on Rice Seed Physiological and Sanitary Quality

2019 ◽  
Vol 11 (15) ◽  
pp. 106
Author(s):  
Alana Emanoele Pereira ◽  
Weliton Lucas da Silva Benites ◽  
Luana de Carvalho Catelan ◽  
Ana Paula Silva ◽  
Nadia Graciele Krohn
Keyword(s):  
Seed Quality ◽  
Dry Weight ◽  
Seed Vigor ◽  
Rice Seed ◽  
Water Control ◽  
Randomized Design ◽  
Seed Health ◽  
Physiological Quality ◽  
Rice Seeds

This study aimed to evaluate the efficiency of integrated biological and chemical control of pathogens in rice seeds and their effects on seed quality. The experiment was conducted in a 2 × 5 factorial completely randomized design. Fungicide-treated (carboxin/thiram) and untreated rice seeds were inoculated with distilled water (control), Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens, or Trichoderma harzianum. Seed vigor and viability, shoot and root length, and seedling dry weight were determined. The blotter test was carried out to assess seed health. Fungicide treatment improved seed vigor and viability and reduced the incidence of fungi. Biological treatment did not enhance the physiological quality of seeds but was able to control fungi. A. brasilense, B. subtilis, P. fluorescens, and T. harzianum controlled Phoma sorghina; B. subtilis, P. fluorescens, and T. harzianum were effective against Aspergillus flavus; P. fluorescens and T. harzianum controlled Pyricularia oryzae; and T. harzianum was effective against Gerlachia oryzae.

scholarly journals O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ

2021 ◽  
Vol 36 (1) ◽  
pp. 28-40
Author(s):  
Jaquelini Garcia ◽  
Cileide Maria Medeiros Coelho
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Mechanical Damage ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Randomized Design ◽  
Physiological Quality ◽  
Drying System

O GRAU DE UMIDADE NA COLHEITA E O SISTEMA DE SECAGEM SÃO DETERMINANTES PARA O VIGOR DE SEMENTES DE ARROZ   JAQUELINI GARCIA1, CILEIDE MARIA MEDEIROS COELHO1   1Programa de Pós-graduação em Produção vegetal, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, 2090, Conta Dinheiro, 88520-000, Lages, SC, Brasil, [email protected], [email protected]   RESUMO: O grau de umidade na colheita e o sistema de secagem podem ser determinantes na qualidade das sementes produzidas. Neste trabalho foi avaliado se o sistema de secagem e o grau de umidade na colheita interferem na qualidade fisiológica das sementes de arroz. Foi realizado em delineamento inteiramente casualizado em esquema fatorial triplo. Foi utilizada seis cultivares produzidas no Alto Vale do Itajaí/SC na safra 2016/17 com grau de umidade na colheita igual ou superior a 17% (19,30 - 17,30%) e inferior a 17% (16,40 - 15,03%) (base úmida). As sementes foram submetidas aos sistemas de secagem estacionário e intermitente. Realizou-se testes de germinação, viabilidade, vigor pelo envelhecimento acelerado e frio. Sementes colhidas com grau de umidade ≥ 17% apresentaram maior vigor. A cultivar foi um fator de grande relevância na sensibilidade ao dano térmico e mecânico. O sistema de secagem não afetou a viabilidade das sementes, com exceção da cultivar SCS122 Miura que foi sensível a dano térmico e mecânico. A secagem estacionária comprometeu a qualidade fisiológica. Conclui-se que a secagem intermitente e a colheita com grau de umidade igual ou superior a 17%, mantém a qualidade fisiológica das cultivares SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru e SCS121 CL.   Palavras-chaves: Secagem estacionária, secagem intermitente, qualidade fisiológica.   THE DEGREE OF MOISTURE IN THE HARVEST AND THE DRYING SYSTEM ARE DETERMINANT FOR THE RICE SEED VIGOR   ABSTRACT: The degree of moisture at harvest and the drying system can determine the quality of the seeds produced. In this work, it was evaluated whether the drying system and the degree of moisture at harvest affect the physiological quality of rice seeds. It was conducted in a completely randomized design in a triple factorial scheme. Six cultivars produced in Alto Vale do Itajaí/SC in the 2016/17 crop were used, with moisture content at harvest equal or greater than 17% (19.30 – 17.30%) and less than 17% (16,40 - 15,03%) (wet basis). Seeds were submitted to stationary and intermittent drying systems. Tests of germination, viability, vigor by accelerated aging and cold were performed. Seeds harvested with moisture content ≥ 17% showed greater vigor. The cultivar was a factor of great relevance in the sensitivity to thermal and mechanical damage. The drying system did not affect seed viability, except for the cultivar SCS122 Miura, which was sensitive to thermal and mechanical damage. Stationary drying compromised physiological quality. It was concluded that intermittent drying and harvesting with moisture content equal or greater than 17% maintain the physiological quality of the SCSBRS Tio Taka, SCS122 Miura, SCS116 Satoru and SCS121 CL cultivars.   Keywords: Stationary drying, intermittent drying, physiological quality.

scholarly journals Assessment of the vigor of rice seeds by near-infrared hyperspectral imaging combined with transfer learning

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44149-44158
Author(s):  
Yong Yang ◽  
Jianping Chen ◽  
Yong He ◽  
Feng Liu ◽  
Xuping Feng ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Transfer Learning ◽  
Significant Role ◽  
Near Infrared ◽  
Seed Vigor ◽  
Rice Production ◽  
Rice Seed ◽  
Rice Seeds

Rice seed vigor plays a significant role in determining the quality and quantity of rice production.

scholarly journals OsGRETCHENHAGEN3-2 modulates rice seed storability via accumulation of abscisic acid and protective substances

2021 ◽  
Author(s):  
Zhiyang Yuan ◽  
Kai Fan ◽  
Yuntong Wang ◽  
Li Tian ◽  
Chaopu Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Molecular Mechanisms ◽  
Genome Wide Association Study ◽  
Seed Viability ◽  
Seed Vigor ◽  
Negative Regulator ◽  
Artificial Ageing ◽  
Rice Seed ◽  
A Genome ◽  
Seed Storability

Abstract Seed storability largely determines the vigor of seeds during storage and is significant in agriculture and ecology. However, the underlying genetic basis remains unclear. In the present study, we report the cloning and characterization of the rice (Oryza sativa) indole-3-acetic acid (IAA)-amido synthetase gene GRETCHEN HAGEN3-2 (OsGH3-2) associated with seed storability. OsGH3-2 was identified by performing a genome-wide association study in rice germplasms with linkage mapping in chromosome substitution segment lines, contributing to the wide variation of seed viability in the populations after long periods of storage and artificial ageing. OsGH3-2 was dominantly expressed in the developing seeds and catalyzed IAA conjugation to amino acids, forming inactive auxin. Transgenic overexpression, knockout and knockdown experiments demonstrated that OsGH3-2 affected seed storability by regulating the accumulation level of abscisic acid. Overexpression of OsGH3-2 significantly decreased seed storability, while knockout or knockdown of the gene enhanced seed storability compared with the wild type. OsGH3-2 acted as a negative regulator of seed storability by modulating many genes related to the abscisic acid pathway and probably subsequently late embryogenesis-abundant proteins at the transcription level. These findings shed light on the molecular mechanisms underlying seed storability and will facilitate the improvement of seed vigor by genomic breeding and gene-editing approaches in rice.

scholarly journals Application of Invigoration Technique in Order to Improve Seed

2020 ◽  
Vol 39 (2) ◽  
pp. 96
Author(s):  
Mira Landep Widiastuti ◽  
Sri Wahyuni
Keyword(s):  
Hybrid Rice ◽  
Growth Yield ◽  
Seed Vigor ◽  
Production Costs ◽  
Yield Reduction ◽  
Rice Seed ◽  
Hormonal Priming ◽  
Rice Seeds ◽  
On Farm ◽  
Priming Technique

<p>Seed is a component of production that contributes to increasing the national rice production. The use of low-quality seeds compared with production costs, non-uniform plant growth, yield reduction, and yield quality. One effort to improve the quality of seed vigor is through invigoration treatment. Invigoration technique among other hydration, thermal treatment, and coating. The hydration method is a water absorption system in seeds. Uncontrolled absorption (hydro-priming), by soaking and drying the seeds, hardening, on-farm priming, soaking for a certain period. The controlled system (osmo-priming) by immersing in osmotic solution, osmo-hardening (integration of hardening and osmo-priming), matri-priming with moist solid media, humidifications (with high humidity), and hormonal priming (with hormones. The second method is by temperature treatment, including by cooling and heating. The third technique is the coating treatment, namely coating the seeds. Hydration system hydro-priming for 30 hours can break seed dormancy with normal germination capacity of 91.3-99.3% in Memberamo, Cipunagara, and Maros varieties. The hardening technique is effective in increasing the germination of hybrid rice seeds for 12 hours with 2 cycles and for 24 hours with one cycle. The osmo-priming technique of soaking seeds in a 10 ppm GA3 solution or a mixture of 10 ppm GA3 + 15 ppm kinetin was effective in increasing the vigor of hybrid rice seeds. The matri-priming technique with rubbing ash as a medium plus the addition of 50 µ m GA3 was effective in breaking dormancy of the Membramo, Cipunagara, and Maros varieties of rice. It is can be applied, especially by small farmers.</p><p>Keywords: Rice, seed, invigoration, quality</p><p> </p><p><strong>Abstrak</strong></p><p><strong>PENERAPAN TEKNIK INVIGORASI DALAM MENINGKATKAN VIGOR BENIH PADI </strong></p><p>Benih merupakan komponen budi daya yang berkontribusi dalam meningkatkan produksi padi nasional. Penggunaan benih bermutu rendah berpengaruh terhadap penambahan biaya produksi, pertumbuhan tanaman tidak seragam, dan penurunan hasil dan mutu hasil. Salah satu upaya untuk meningkatkan mutu benih dari aspek vigor yang rendah adalah melalui perlakuan invigorasi. Teknik invigorasi dibagi menjadi tiga metode, yaitu hidrasi, perlakuan suhu, dan pelapisan (coating). Metode hidrasi merupakan sistem penyerapan air yang dapat terjadi pada benih. Penyerapan tidak terkontrol (hydro-priming) yaitu dengan merendam dan mengeringkan benih, pengerasan (hardening), on-farm priming yaitu merendam dalam jangka waktu tertentu. Sedangkan terkontrol (osmo-priming) dengan merendam pada larutan osmotik, osmo-hardening (integrasi hardening dan osmo-priming), matripriming dengan media padat lembab, humidifications (dengan kelembaban tinggi), dan hormonal priming (dengan hormon). Metode kedua adalah dengan perlakuan suhu, diantaranya dengan pendinginan dan pemanasan. Teknik ketiga adalah dengan perlakuan coating yaitu melapisi benih. Sistem hidrasi hydropriming selama 30 jam dapat mematahkan dormansi benih dengan daya kecambah normal 91,3-99,3% pada varietas Memberamo, Cipunagara, dan Maros. Teknik hardening efektif meningkatkan daya berkecambah benih padi hibrida selama 12 jam dengan dua siklus dan selama 24 jam dengan satu siklus. Teknik osmo-priming perendaman benih dalam larutan GA3 10 ppm atau larutan campuran GA3 10 ppm + kinetin 15 ppm efektif meningkatkan vigor benih padi hibrida. Teknik matri-priming dengan abu gosok sebagai media plus penambahan GA3 50 µm efektif mematahkan dormansi padi varietas Membramo, Cipunagara, dan Maros. Teknik invigorasi tersebut dapat diterapkan, khususnya oleh petani dengan skala produksi kecil.</p><p>Kata kunci: Padi, benih, invigorasi, mutu</p>

scholarly journals Production of Hybrid Rice seeds using environment sensitive genic male sterile (EGMS) and basmati rice lines in Kenya

2019 ◽  
Author(s):  
Njiruh Paul Nthakanio ◽  
Kariuki Simon Njau
Keyword(s):  
High Temperature ◽  
Hybrid Rice ◽  
Male Gamete ◽  
Growth Conditions ◽  
Basmati Rice ◽  
Rice Seed ◽  
Male Sterile ◽  
Male Gametes ◽  
Rice Seeds ◽  
The Impact

AbstractPhotoperiod-sensitive genic male sterile rice (PGMS) lines IR-73827-23-76-15-7 S, IR-75589-31-27-8-33S referred to as P1 and P2, and IR-77271-42-25-4-36S, thermo-sensitive genic male sterile (TGMS) line referred to as T were obtained from International Rice research Institute. These lines, collectively known as environment genic male sterile lines, were sown under greenhouse growth conditions where temperatures were more than 34°C with an objective of inducing complete male gamete sterility in them. Results indicated that high temperature growth conditions induces complete male gamete sterility in both the PGMS and TGMS lines. The impact of this is that, it will be possible to produce pure basmati hybrid rice seed in the tropical regions without contamination with pure breed lines. The male sterile PGMS/TGMS were pollinated with pollen from basmati370 and 217 grown under natural conditions and some hybrid seeds were obtained. This shows that high temperature emasculated the male gametes but not female ones. The conclusion is that it is possible to induce complete male gamete sterility in PGMS and TGMS under greenhouse in tropical growth conditions, and to produce hybrid rice seeds. This makes basmati hybrid rice seed production in Kenya a viable venture.

scholarly journals Methodological adjustments to the tetrazolium test in rice seeds

2017 ◽  
Vol 39 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Ireni Leitzke Carvalho ◽  
Géri Eduardo Meneghello ◽  
Lilian Madruga de Tunes ◽  
Caroline Costa Jácome ◽  
Vanessa Nogueira Soares
Keyword(s):  
Salt Concentration ◽  
Execution Time ◽  
Seed Viability ◽  
Tetrazolium Salt ◽  
Rice Seed ◽  
The Third ◽  
Evaluation Time ◽  
Two Temperatures ◽  
Rice Seeds ◽  
Tetrazolium Test

Abstract: Reducing the execution time of the tetrazolium test is important because it is used for making decisions during the preharvest and this test takes approximately 24 h. Thus, the goal of this research was to study preconditioning and staining periods and concentration of tetrazolium salt, in order to reduce the evaluation time of rice seed viability by tetrazolium test. Three independent experiments were conducted. In the first and second experiments, six rice seed lots from the BR Irga 424 cultivar were used. In the first experiment, different concentrations of tetrazolium salt (0.1, 0.25, 0.5 and 1 %) and staining times (0.5, 1.0 and 2.0 h) were evaluated. In the second one, different hydration periods (0.5, 1.0, 2.0 and 4.0 h) at two temperatures (35 and 40 °C) were tested. In the third one, eight seed lots from the same cultivar were used, and the effectiveness of the modified tetrazolium test in the evaluation of rice seed viability was assesed. It is possible to carried out the tetrazolium test on rice using the hydration of peeled seeds for 1 h at 40 °C and staining for 1 h with a 0.25% salt concentration.

Sign in / Sign up

Export Citation Format

Share Document