scholarly journals Efektivitas Seed Coating dan Biopriming dengan Rizobakteri dalam Mempertahankan Viabilitas Benih Cabai dan Rizobakteri selama Penyimpanan

2018 ◽  
Vol 8 (3) ◽  
pp. 192
Author(s):  
Ita Madyasari ◽  
Candra Budiman ◽  
Syamsuddin , ◽  
Dyah Manohara ◽  
Satriyas Ilyas
Keyword(s):  
Sodium Alginate ◽  
Seed Treatment ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Vigor ◽  
Hot Pepper ◽  
Seed Coating ◽  
Control Seed ◽  
Randomized Design ◽  
Two Factors

<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>The objective of the study was to obtain the best coating formula for hot pepper seeds, and evaluate the effect of seed coating and biopriming with rhizobacteria on viability of hot pepper seeds and rhizobacteria during storage. </em><em>Experiment 1 was arranged in a completely randomized design with one factor i.e. 11 coating formula. Experiment 2 was arranged in a nested plot design with two factors, storage period (0, 4, 8, 12, 16, 20, and 24 weeks) as main factor and seed treatment consisted of 11 treatments (control, seed coating with E1+F2B1, ST116B, CM8; biopriming 24 h with E1+F2B1, ST116B, CM8; biopriming 48 h with E1+F2B1, ST116B, and CM8; priming metalaxyl) as nested factor. Result of experiment 1 indicated that the</em><em> best coating formula for hot pepper seed was sodium alginate 2.5% and was used in experiment 2. </em><em>Experiment 2 showed that seed coating and biopriming with rhizobacteria were able to maintain seed viability (79-89%) for 24 weeks of storage at 27-30 <sup>0</sup>C as compared to priming metalaxyl (54%). Biopriming E1+F2B1 24 h or CM8 48 h resulted in the highest index of seed vigor after 24 weeks of storage. Population of rhizobacteria in seed tissue decreased in bioprimed seeds from 10<sup>5</sup>-10<sup>7</sup> cfu g<sup>-1</sup> to 10<sup>4 </sup>cfu g<sup>-1</sup> after being stored for 24 weeks. </em></p><p><em>Keywords: rhizobacteria isolates, seed treatment, seed vigor, sodium alginate</em></p><p><em> </em></p><p align="center"><strong>ABSTRAK <br /></strong></p><p>Penelitian ini bertujuan mendapatkan formula <em>coating</em> terbaik pada benih cabai dan mengevaluasi pengaruh <em>seed coating</em> dan <em>biopriming</em> dengan rizobakteri dalam mempertahankan viabilitas benih cabai dan rizobakteri selama penyimpanan. Percobaan 1 menggunakan rancangan acak lengkap satu faktor yang terdiri atas 11 formula <em>coating</em>. Percobaan 2 menggunakan rancangan petak tersarang dua faktor, periode simpan (0, 4, 8, 12, 16, 20, dan 24 minggu) sebagai faktor utama dan perlakuaan benih yang terdiri atas 11 perlakuan (kontrol, <em>seed coating </em>dengan E1+F2B1<em>, </em>ST116B<em>, </em>CM8<em>; biopriming </em>24 jam dengan E1+F2B1<em>, </em>ST116B, CM8; <em>biopriming </em>48 jam dengan E1+F2B1<em>, </em>ST116B, dan CM8<em>; priming </em>metalaksil)<em> </em>sebagai faktor tersarang<em>.</em> Hasil Percobaan 1 menunjukkan bahwa formula <em>coating</em> terbaik untuk benih cabai ialah natrium alginat 2.5% dan digunakan pada percobaan 2. Percobaan 2 menunjukkan bahwa <em>seed coating</em> dan <em>biopriming</em> dengan rizobakteri mampu mempertahankan viabilitas benih (78-89%) selama 24 minggu penyimpanan pada suhu 27-30 <sup>0</sup>C<em> </em>dibandingkan <em>priming</em> metalaksil (54%). <em>Biopriming</em> E1+F2B1 24 jam atau <em>biopriming</em> CM8 48 jam menghasilkan indeks vigor paling tinggi setelah disimpan selama 24 minggu. Populasi rizobakteri di dalam jaringan benih menurun pada benih yang diberi perlakuan <em>biopriming</em> dari 10<sup>5</sup>-10<sup>7</sup> cfu g<sup>-1</sup> menjadi 10<sup>4 </sup>cfu g<sup>-1</sup> setelah disimpan selama 24 minggu.</p><p>Kata kunci: isolat rizobakteri, natrium alginat, perlakuan benih, vigor</p>

scholarly journals Aplikasi Bakteri Probiotik untuk Meningkatkan Mutu Fisiologi dan Kesehatan Bibit Cabai (Capsicum annuum L.)

2016 ◽  
Vol 44 (2) ◽  
pp. 176 ◽  
Author(s):  
Anna Tefa ◽  
Eny Widajati ◽  
Muhamad Syukur ◽  
Dan Giyanto
Keyword(s):  
Storage Period ◽  
Seed Storage ◽  
Colletotrichum Acutatum ◽  
Seed Coating ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Seedling Vigour ◽  
Chilli Pepper ◽  
Control Seed ◽  
Randomized Design

ABSTRACT<br /><br />The use of probiotic bacteria as biocontrol agents is one of the methods of controlling anthracnose disease caused by Colletotrichum acutatum. The objective of this research was to suppress the infection of C. acutatum and increase chilli pepper seedling vigour. The research involved factorial experiments arranged in a completely randomized design with two factors. The first factor was seed coating involving six treatments, i.e., control, seed coating without bacteria, seed coating with Bacillus sp., Pseudomonas sp., Actinomycetes sp, and fungicide. The second factor was the seed storage period where six storage periods were experimented, i.e., 0, 1, 2, 3, 4, 5, and 6 months. The results showed that the coating treatment of Bacillus sp., Pseudomonas sp., and Actinomycetes sp. improved germination, growth rate and number of leaves and reduced the incidence of attacks and infection of hypocotyls at 5 month storage period.<br /><br />Keywords: Actinomycetes sp., Bacillus sp., Pseudomonas sp., seed coating, storage period

scholarly journals Perilaku Biokimia Benih Kedelai Selama Penyimpanan dalam Kondisi Terkontrol

2017 ◽  
Vol 44 (3) ◽  
pp. 255
Author(s):  
Irma Noviana ◽  
Abdul Qadir ◽  
Dan Faiza Chairani Suwarno
Keyword(s):  
Electrical Conductivity ◽  
Peroxide Value ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Biochemical Changes ◽  
Soybean Seeds ◽  
Controlled Conditions ◽  
Randomized Design

ABSTRACT<br /><br />Physiological and biochemical changes in seeds indicates levels of viability and vigor during deterioration. The aims of the research were to study the patterns of biochemical changes in soybean seeds and its relationship to seed vigor during storage under controlled conditions. The experiment was arranged in a completely randomized design using two varieties as a factor which was nested into storage period and replicated four times. Two varieties were Gepak Kuning and Mallika, stored in controlled condition at temperature of 19-22 °C and 64-67% of relative humidity for six months. The seeds were evaluated for protein, peroxide value, electrical conductivity and seed viability. The exponential regression analysis was used to determine the patterns of biochemical changes of each soybean varieties during the storage period. The results showed that the biochemical behaviour of soybean seeds stored under controlled conditions for 24 weeks following the exponential equation of y = a + b expcx, which a, b, and c were constants that has different value for each variety. Electrical conductivity and peroxide value could be used as indicators of seed vigor during storage under controlled conditions for 24 weeks. Seed storage under controlled conditions are to maintain seed vigor &gt;80% up to 12 weeks for Mallika and 24 weeks for Gepak Kuning with low oxidative process and less membrane damages.<br /><br />Keywords:deterioration, storability, vigor<br /><br />

scholarly journals PENGARUH KONSENTRASI ASAM GIBERELAT (GA ) DAN LAMA PERENDAMAN TERHADAP VIABILITAS, VIGOR dan 3 PERTUMBUHAN BENIH JARAK (Jatropha curcas L.) KLON IP-1P di PEMBENIHAN

2017 ◽  
Vol 3 (2) ◽  
pp. 9
Author(s):  
Asep Samsul Mustopa
Keyword(s):  
Block Design ◽  
Seed Viability ◽  
Plant Biotechnology ◽  
Seed Vigor ◽  
Growth And Yield ◽  
Root Number ◽  
Soaking Time ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves

The experiment was carried out at Plant Biotechnology laboratorium and experimentalfield Faculty of Agriculture University of Winaya Mukti Tanjungsari Sumedang which the altitude is about 850 meters above sea level, from March 2008 until May 2008.Theobjective of this experiment was to studying the interaction effect of giberellat acidconcentration and and soaking time on seed viability, seed vigor, growth and yield ofJatropha curcas in nursery.Design used was Randomized Block Design (RBD) withfactorial pattern, treatment consisted of two factors and two replications. The first factorwas giberellat acid (GA3) concentration (K) which consisted of 4 levels namely : k1=250 ppm, k2= 500 ppm, k3= 750 ppm and k4= 1000 ppm. The second factor soakingtime on GA34 (M) which consisted of 4 levels namely : m1= 6 hour, m2= 18 hour dan m = 24 hour.The results of these experiment showed there was aninteraction between giberellat acid concentration and and soaking time on high of plant20 day after planting (DAP), 40 DAP, 60 DAP, number of leaves 20 DAP, 40 DAP, 60DAP, length of root, number of root, dry wight of root and dry wight of shoot. 

scholarly journals Pengaruh Konsentrasi Asap Cair Bambu Tabah (Gigantochloa nigrociliata Buse-Kurz) dan Lama Perendaman Terhadap Kesegaran Pisang Cavendish (Musa Acuminata)

2021 ◽  
Vol 9 (1) ◽  
pp. 45
Author(s):  
K Justikha Natalia Praja ◽  
Pande Ketut Diah Kencana ◽  
I Gusti Ketut Arya Arthawan
Keyword(s):  
Weight Loss ◽  
Storage Temperature ◽  
Storage Period ◽  
Soaking Time ◽  
Average Value ◽  
Liquid Smoke ◽  
Randomized Design ◽  
Two Factors ◽  
Four Levels ◽  
Natural Preservatives

ABSTRAK Buah pisang Cavendish merupakan salah satu produk hortikultura yang rentan mengalami kemunduran fisiologis dan sangat rentan terhadap kerusakan fisik setelah panen. Penggunaan berbagai konsentrasi asap cair bambu tabah dengan lama perendaman yang berbeda dapat digunakan sebagai pengawet alami. Buah pisang yang digunakan adalah pisang Cavendish yang berasal dari Kabupaten Buleleng, dalam satu sisir berisi paling sedikit10 buah dengan panjang rata-rata 18.89 cm dan berat rata-rata 125  gram (g). Tujuan penelitian ini adalah menemukan kombinasi perlakuan terbaik dari perlakuan konsentrasi asap cair dan lama perendaman untuk menjaga kesegaran dan masa simpan pada buah pisang Cavendish selama penyimpanan. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) faktorial dengan dua faktor, yaitu faktor pertama adalah konsentrasi asap cair bambu tabah dan faktor kedua adalah lama perendaman. Faktor pertama terdiri dari empat taraf konsentrasi yaitu 1%, 3%, 5%, dan 7%. Faktor kedua terdiri dari tiga taraf lama perendaman yaitu 10 menit, 20 menit dan 30 menit dengan suhu penyimpanan menggunakan suhu ruang (28±2oC).  Parameter yang diamati dalam penelitian ini adalah laju respirasi, susut bobot, kekerasan, TPT, tingkat kerusakan, dan organoleptik. Hasil interaksi terbaik ditunjukkan pada perlakuan 7% konsentrasi asap cair bambu tabah dan perendaman 10 menit, dengan nilai rata-rata parameter laju respirasi 51.18 ml CO2/kg.jam, nilai rata-rata susut bobot dengan nilai 17.61%, nilai rata-rata kekerasan 3.82 kg, nilai rata-rata TPT 15.65 %Brix, nilai tingkat kerusakan 2.40, nilai rata-rata organoleptik rasa sebesar 4.67, aroma sebesar 4.54 dan warna sebesar 3.87. Disimpulkan bahwa kombinasi perlakuan A4B1 (konsentrasi asap cair bambu tabah 7% dengan perendaman selama 10 menit) adalah kombinasi terbaik untuk menjaga kesegaran dan memperlambat penurunan kualitas buah pisang Cavendish hingga akhir periode penyimpanan hari ke-15. ABSTRACT Cavendish banana one of the horticultural products that are prone to physiological setbacks and very vulnerable to physical damages after harvest. Various concentrations of tabah bamboo liquid smoke with different soaking times can use as natural preservatives. The bananas used in this study were Cavendish bananas from Buleleng, every bunch consists of at least ten banana with a length of 18.59 cm and a weight of 125 ± 5 g. This study aims to find the best treatment combination of the treatmentoof liquid smoke concentration and soaking time to maintain freshness and shelf life of Cavendish bananas during storage. The experimental design used in this study was a Completely Randomized Design (CRD) with two factors, the first factor was the concentration of tabah bamboo liquid smoke, and the second factor was the soaking time. The first factor consists of four levels of concentration namely 1%, 3%, 5%, and 7%. The second factor consists of three levels of soaking time, namely 10 minutes, 20 minutes and 30 minutes with storage temperature using room temperature (28 ± 2oC).  The parameters observed in this study are: repiration rate, weight loss, hardness, level of damages, TPT and organoleptics. The best interaction results are shown in the treatment of 7% concentration of tabah bamboo liquid smoke with 10 minutes of soaking with an average value of respiration rate parameters of 51.18 ml CO2 / kg.hour, an average value of weight loss with a value of 17.61%, an average value of hardness of 3.82 kg, the average value of TPT was 15.65% Brix, the value of the level of damage was 2.40, the average organoleptic value of taste was 4.67, aroma was 4.54 and color was 3.87. The conclus of this study indicates that the combination of A4B1 (7% tabah bamboo liquid smoke concentration with soaking for 10 minutes) is the best combination to keep freshness and the quality of green bananas until the storage period of the 15th day.

scholarly journals Effects of different post-harvest treatments on bio-chemical characters and diseases of litchi in storage

2020 ◽  
Vol 8 (1) ◽  
pp. 57
Author(s):  
Fakhar Uddin Talukder ◽  
Md. Sohanur Rahman ◽  
Md. Kamrul Hassan
Keyword(s):  
Low Temperature ◽  
Vitamin C ◽  
Ambient Temperature ◽  
Disease Incidence ◽  
Storage Period ◽  
Post Harvest ◽  
Randomized Design ◽  
Two Factors ◽  
Soluble Solid ◽  
Chemical Characters

Objective of the study was to evaluate the effects of different post-harvest treatments on bio-chemical characters and diseases of litchi (Litchi chinensis Sonn, var. Bombai). The experiment consisted of two factors. Factor A: Temperature viz. T1: Ambient temperature, T2: 4ºC temperature; Factor B: PP bags (Polypropylene bag) viz. P1: Control (unwrapped), P2: 50 micro meter (µm) PP bag, P3: 75µmm PP bag, P4: 100µm PP bag. The experiment was conducted in completely randomized design (CRD) with three replications. Significant variation was observed in total soluble solid, PH of fruit pulp, vitamin c content, percent disease incidence and disease severity during the storage period. TSS contents increased up to the 6th day of storage and there after declined. pH values were maximum (4.14) in the fruits kept in 100µ polypropylene bag at ambient temperature. Vitamin C continent decreased with the increase of storage period. Disease incidence and severity progress with the storage period. Among the treated and untreated fruits, 75µm pp bag at low temperature (4ºC) treatment exhibited better storage performance. More research should be conducted by using other litchi cultivar like Bedana, China-3 etc. Various technologies have been devised to minimize the post-harvest losses of litchi, one of such technologies is the use of PP bag & low temperature.  

scholarly journals Keefektifan Pelapisan Benih terhadap Peningkatan Mutu Benih Padi Selama Penyimpanan

2015 ◽  
Vol 34 (2) ◽  
pp. 145
Author(s):  
Ikrarwati Ikrarwati ◽  
Satriyas Ilyas ◽  
AmiyarsI Mustika Yukti
Keyword(s):  
Pathogenic Bacteria ◽  
Seed Viability ◽  
Seed Coating ◽  
Rice Seed ◽  
Clove Oil ◽  
Arabic Gum ◽  
Control Seed ◽  
Citronella Oil ◽  
Lemon Grass ◽  
Four Levels

Seed coating using biological pesticides such as clove oil and citronella oil, was expected to have the ability to control seed-borne pathogens on rice. The aim of the research was to determine the effectiveness of seed coating using clove oil and lemon grass oil against seed-borne fungi and bacteria on rice seed variety “Hipa 8” during storage. The experiment was conducted in Cimanggis, from February to September 2012, using completely randomized design with single factor, consisting of four levels: (1) clove oil 1% + chitosan 3%; (2) lemon grass oil 2% + carboxymethyl-cellulose 1%; (3) Synthetic pesticide (streptomycin sulphate 0.04% + benomyl 0.1%) + arabic gum 10%; and (4) control (without coating). Results showed that seedborne fungi pathogens on seed of Hipa 8 rice were detected at 6-month storage consisted of Fusarium sp., Curvularia sp., Alternaria sp., Cladosporium sp., Aspergillus sp. and Penicillium sp. The detected pathogenic bacteria were Xanthomonas oryzae pv. oryzae and X. campestris pv. oryzicola. Coating formula most compatible with the rice seed of HIPA 8 was chemical pesticide + 10% arabic gum, which suppressed fungal infection from 80% to 45% at the first month and from 90% to 70% at the fifth month, suppressed populations of Xoo + Xco from 7.6 x 108 cfu/g of seed to 5.86 x 105 cfu/g of seed at the first month and 7.0 x 106 cfu/g of seed to 1.4 x 104 cfu/g of seeds at the sixth month. The treatment caused the smallest decrease of seed viability compared to the other coating treatments.

scholarly journals TEOR DE PROTEÍNAS EM SEMENTES DE SOJA PRODUZIDAS EM SOLOS DE VÁRZEA COMO USO DE BIOREGULADOR

2018 ◽  
Vol 14 (4) ◽  
pp. 135-144
Author(s):  
Sandro Oliveira ◽  
Cristiano Dietrech Ferreira ◽  
Elisa Souza Lemes ◽  
Jerffeson Araujo Cavalcante ◽  
Géri Eduardo Meneghello ◽  
...  
Keyword(s):  
Protein Content ◽  
Soluble Protein ◽  
Seed Treatment ◽  
Total Protein Content ◽  
Seed Coating ◽  
Soluble Protein Content ◽  
Experimental Field ◽  
Randomized Design ◽  
Mode Of Application ◽  
Seed Harvest

This study aimed to evaluatethe protein content in soybean seeds produced in lowland soil from plant growth regulator applied via seed coating and leaf spraying. The study wasdivided into two experiments: Seeds coating (Experiment I) and leaf spraying (Experiment II) with different Stimulate® doses. Two soybean cultivars were used (BMX Potência RR and Fundacep 64 RR) and the experimental model used was a completely randomized design. For the seed coating, the doses used was0; 250; 500; 750 e 1000 mL 100 kg seeds-1, and for the leaf spraying was 0; 187.5; 375.0; 652.5 e 750.0 mL ha-1. The experiments were carried out during the 2013/2014 season at experimental field and pots. In the maturity period was performed the seed harvest, afterward determined the total and soluble protein content. The bioregulator doses appliedin seed treatment, up tothe highestdose,increase the crude protein content of seeds produced in pots of BMX Potência RRcv.When produced in the experimental field, doses of bioregulator in seed treatment and leaf spraying do not promote difference in the protein content of the seeds. Increases were observed in the total protein content in Fundacep 64 RR cultivar, and reduction in soluble protein content in BMX Potência RR with the use of bioregulator, regardless of the mode of application.

scholarly journals Pengaruh Dosis Minyak Cengkeh Terhadap Populasi Hama Callosobruchus chinensis L., Viabilitas Dan Vigor Benih Dua Kultivar Kacang Hijau Setelah Periode Simpan Tiga Bulan

Agrologia ◽  
2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Siti Syarah Nurbaekah ◽  
Sumadi Sumadi ◽  
Anne Nuraini
Keyword(s):  
Mung Bean ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Vigor ◽  
Callosobruchus Chinensis ◽  
Clove Oil ◽  
Bean Cultivar ◽  
Randomized Design ◽  
Vigor Index ◽  
Completely Randomized Design

Callosobruchus chinensis L. still become the primary pest of mung bean, it can cause damage until 70 percent. Pest control on mung bean seeds can be done by using anorganic protectant and bio protectant. The aim of the research was to determine the dosage of clove oil that could suppressed the population of weevil C. chinensis L. and maintaining seed viability and seed vigor of two mung bean cultivar after three months storage. The experimental design was Completely Randomized Design with 10 treatments combination of cultivar and clove oil dosage; all treatment were replicated three times. The mungbean seeds were cultivar Vima 1 and Vima 3.  The clove oil dosages used were 0 mL kg-1, 0,5 mL kg-1, 1,0 mL kg-1, 1,5 mL kg-1 and 2,0 mL kg-1. The mungbean result of experiment showed that there were significant effect between cultivar and clove oil dosage on germination rate percentage after three months storage, but no significant effect on weevil population and vigor index. The dosage of 1,5 mL kg-1 clove oil was the better treatment to maintaining seed viabilty andits vigor.Keywords: Mung bean, C. chinensis L., Clove oil, Seed viability and Seed Vigor.

scholarly journals Effects of pre-sowing seed treatment with KCl on yield and quality of two peanut cultivars L12 and L18

2021 ◽  
Vol 20 (1) ◽  
pp. 1-9
Author(s):  
Trong V. Le
Keyword(s):  
Seed Treatment ◽  
Starch Content ◽  
Mineral Elements ◽  
Yield And Quality ◽  
Saponification Value ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Sowing Seed

The study was conducted to evaluate the effect of 0.05% KCl on yield and quality of two peanut varieties L12 and L18 grown in Thanh Hoa province. The experiment was arranged in a completely randomized design with two factors (varieties and chemicals). After careful selection, L12 and L18 seeds were divided into two parts. Part 1 was treated with distilled water as control and part 2 was treated with 0.05% KCl. The results showed that pre-sowing seed treatment with 0.05% KCl increased the yield components and yield of both L12 and L18 when compared to the control, in which the yield of L18 in both treatments reached 37.37 quintals/ha and 39.54 quintals/ha and was higher than that of the L12 variety at 35.77 quintals/ha and 36.40 quintals/ha. Pre-sowing seed treatment with 0.05% KCl also increased the quality of peanuts such as starch content, reducing sugar, lipid, saponification value, protein, B vitamins, total amino acids and content of some mineral elements in peanuts such as N, K, Ca, Mg. Briefly, the results of this study indicated that pre-sowing seed with KCl increased the yield and quality of peanuts.

scholarly journals Karakter Habitus dan Viabilitas Biji Tanaman Biduri (Calotropis gigantea) Aksesi Lombok Tengah Bagian Selatan

2021 ◽  
Vol 7 (1) ◽  
pp. 114-133
Author(s):  
Yuliani Ahmad ◽  
M. Taufik Fauzi ◽  
Bambang Budi Santoso
Keyword(s):  
Fruit Size ◽  
Seed Viability ◽  
Growth Potential ◽  
Calotropis Gigantea ◽  
Survey Techniques ◽  
South Central ◽  
Randomized Design ◽  
Panicle Number ◽  
Seed Growth ◽  
Two Factors

This study aims to describe the morphological character and seed growth potential of biduri (Calotropis gigantea) accession growing in the tourist area of South Central Lombok (Kuta Mandalika, Tanjung Aan, and Seger), and was carried out during February-August 2020. The method used was descriptive observative with survey techniques and sampling by purposive sampling based on the population of biduri. The seed growth experiment used a completely randomized design in a greenhouse consisting of two factors, namely the type of substrate and accession, which was repeated three times, so that there were 18 experimental units. The results shows that the Kuta Mandalika Biduri accession was showed superiority in the number of fruit characters per panicle. Tanjung Aan accessions excel in branching characters (primary and secondary branches), number of panicles per tree, number of flowers per panicle, number of fruits per tree and fruit size. Meanwhile, Seger accessions were superior in terms of plant height, seed size and weight of 100 seeds. There were no character differences in seed viability of the three accessions. The closest genetic-relationship occurs in the Kuta Mandalika accession of plot 2 sample 2 with the Kuta Mandalika accession of plot 3 sample 1 marked with a coefficient value of 1.00. Meanwhile, the furthest relationship was found in the Kuta Mandalika accession of plot 1 sample 1 with Tanjung Aan accession of plot 2 sample 2 marked with a coefficient value of 33.932. 

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