Survey of Keratinophilic Fungi in the Tigris River in Baghdad

The present study includs the isolation and identification of Keratinophilic fungi in fresh water samples collected from Tigris river in Baghdad, Iraq. Samples were collected from different districts of Baghdad (Al-Rustumia, Madina Al-Teb, Al-Azamia, Al-Kadimia, Al-Dorha and Al-Kanah). The water samples were examined for keratinophilic fungi by the plating method culturing and Hair Baiting Technique. A total number of 18 genera are recorded, in which the Tigris water indicates an impressive diversity of fungi. A variety of fungal strains were isolated from river water, out of 814 fungal colonies, Aspergillus terrus (5.5%) was the most frequent species, followed by Aspergillus niger (5.2%) then Rhizopus stolonifer (3.3%). The results revealed that the highest number of fungal species are found in water surface near the margin (75%) followed by the fungi isolated from water surface in the middle of the river (25%), whereas the number of fungi in 50 cm depth from water surface near the margin and 50 cm depth from water surface in the middle of the river is 17.10% and 12.20% species respectively. The results suggest that the Tigris river is a potential transmission route of fungi and a healthily hazard mainly for the immune depressed individual.

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Epidemiological Study of Keratinophilic Fungi in Baghdad Swimming Pools

Baghdad Science Journal ◽

10.21123/bsj.11.3.1122-1129 ◽

2014 ◽

Vol 11 (3) ◽

pp. 1122-1129

Author(s):

Baghdad Science Journal

Keyword(s):

Fungal Infection ◽

Epidemiological Study ◽

Water Samples ◽

Fungal Species ◽

Summer Season ◽

Keratinophilic Fungi ◽

Swimming Pool ◽

Swimming Pools ◽

Isolation Period

The present study was included the isolation of Keratinophilic fungi from water samples taken from 19 indoor public swimming pools in Baghdad during four season (summer ,fall ,spring ,and winter). Isolation period was September 2011and September 2012 . Samples were collected from different places in swimming pool . Sixteen keratinophilic fungal species, belonging to twenty –one genera were isolated using Surface Dilution Plating (SDP) and Hair Bait Technique (HBT) . Aspergillus genus was the most frequent and occurrence (19.84%), followed by Trichophyton (11.60%) and Fusarium (7.59% ) . The most common fungal infection occurred in summer season (42.16%) , and the most frequent month manifestation of species occurred in August (17%) .

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Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2018/v1i2747 ◽

2018 ◽

pp. 1-7

Author(s):

I. Y. Tafinta ◽

K. Sheh ◽

H. M. Maishanu ◽

S. S. Noma ◽

S. A. Yusif ◽

...

Keyword(s):

Aspergillus Niger ◽

Fusarium Oxysporum ◽

Trichoderma Harzianum ◽

Fungal Species ◽

Potato Dextrose Agar ◽

Morphological Features ◽

Rhizopus Stolonifer ◽

Isolation And Identification ◽

Dilution Plate ◽

Cotton Blue

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cultivated soils (A) out of the total 132 isolates. Variations between sites were statistically analyzed.

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Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2020.21.311 ◽

2020 ◽

Vol 21 (3) ◽

pp. 61-66

Author(s):

Ola Hashem ◽

Viola Zaki ◽

Rawia Adawy

Keyword(s):

Molecular Characterization ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Clarias Gariepinus ◽

Fungal Species ◽

High Rate ◽

Fish Farms ◽

Isolation And Identification ◽

Penicillium Spp

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

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Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil

Mycological Progress ◽

10.1007/s11557-021-01674-z ◽

2021 ◽

Vol 20 (3) ◽

pp. 325-339

Author(s):

Frederick Witfeld ◽

Dominik Begerow ◽

Marco Alexandre Guerreiro

Keyword(s):

Elevated Temperatures ◽

Fungal Species ◽

Dna Barcodes ◽

Heat Shock Treatment ◽

Isolation And Identification ◽

Heat Resistant ◽

Heat Stable ◽

Promising Source ◽

Culture Dependent ◽

Selection Of

AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high.

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Filamentous fungi isolated from sand and water of "Bairro Novo" and "Casa Caiada" beaches, Olinda, Pernambuco, Brazil

Brazilian Journal of Biology ◽

10.1590/s1519-69842008000300016 ◽

2008 ◽

Vol 68 (3) ◽

pp. 577-582 ◽

Cited By ~ 26

Author(s):

DNF. Gomes ◽

MAQ. Cavalcanti ◽

MJS. Fernandes ◽

DMM. Lima ◽

JZO. Passavante

Keyword(s):

Water Samples ◽

Water Surface ◽

High Tide ◽

Sand Sample ◽

Distilled Water ◽

Sabouraud Agar ◽

Sand Surface ◽

Heterotrophic Microorganisms ◽

Petri Dishes ◽

Dry And Rainy Seasons

Fungi comprehend a heterogeneous group of heterotrophic microorganisms that act as saprobes or parasites or, less frequently, as symbionts living in association with other organisms. They are cosmopolitan and important components of ecosystems. Considering the small number of Brazilian papers on the filamentous mycota in marine environments, and the need to improve knowledge of the diversity of these microfungi in "Casa Caiada" and "Bairro Novo" beaches, Olinda, PE, the aim of this work was to isolate and identify the fungi from sand and water samples of these ecosystems. Thirty two samplings of sand (surface and 20 cm deep) and water (surface and 1 m deep) were carried out during the dry and rainy seasons, in low and high tide. From each sand sample, a suspension was made with 50 g of sand diluted in 90 mL of sterilized distilled water. From each sand suspension and water sample, 0.5 mL was spread, in triplicate, onto Petri dishes containing Sabouraud Agar added of chloramphenicol. The plates were incubated at 28 °C (±2 °C). Fifty seven species were isolated, identified, and classified in 20 genera. Aspergillus and Penicillium were the most frequent genera in both sand and water, with a total of 11 and 19 species, respectively.

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Isolation and identification of phylloplane and endophytic fungi from Sapindus mukorossi

FLORA AND FAUNA ◽

10.33451/florafauna.v26i2pp218-220 ◽

2020 ◽

Vol 26 (2) ◽

Author(s):

B.D. Varpe

Keyword(s):

Endophytic Fungi ◽

Terrestrial Ecosystem ◽

Fungal Species ◽

Penicillium Expansum ◽

Isolation And Identification ◽

Epicoccum Nigrum ◽

Candida Sp ◽

Fusarium Sp ◽

The Rich ◽

High Level

Phylloplane biodiversity and endophytic fungi is considered one of the rich origins of novel biological activity compounds and high-level structural variation on the leaf surface. Plant leaves surface is a diverse terrestrial ecosystem, including filamentous fungi. This study aims to study the isolation and enumeration of Sapindus mukorossi phylloplane and endophytic fungal diversity. The Sapindus mukorossi isolated 14 fungal species from 9 genera of phylloplane and endophytic fungi. Cladosporium herbarum, Penicillium expansum, Fusarium oxysporum, Fusarium sp., Alternaria alternate, Collectotrichum orbiculare, Torulla herbarium, Epicoccum nigrum and Candida sp. as a phylloplane fungi. Aspergillus niger, A. flavus, Epicoccum nigrum. Penicillum digitatum, Penicillum sp. were identified as endophytic fungi.

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The Efficacy of a Diode Laser on Titanium Implants for the Reduction of Microorganisms That Cause Periimplantitis

Materials ◽

10.3390/ma14237215 ◽

2021 ◽

Vol 14 (23) ◽

pp. 7215

Author(s):

Anna Wawrzyk ◽

Michał Łobacz ◽

Agnieszka Adamczuk ◽

Weronika Sofińska-Chmiel ◽

Mansur Rahnama

Keyword(s):

Laser Irradiation ◽

Diode Laser ◽

Fungal Species ◽

Laser Wavelength ◽

Titanium Implants ◽

Isolation And Identification ◽

Implant Surface ◽

Streptococcus Constellatus ◽

Surface Optical ◽

Irradiation Process

The paper presents the optimisation of a safe diode laser irradiation process applied to the surface of titanium implants in order to reduce microbial numbers in the treatment of inflammation classified as periimplantitis. The study comprised isolation and identification of microorganisms inhabiting surfaces of dental implants, crowns, teeth and saliva from patients with fully symptomatic periimplantitis. Microorganisms were detected by a culture-dependent method and identified with the use of MALDI-TOF mass spectrometry. The isolated microorganisms were inoculated on the surface of a new implant and then irradiated by a diode laser (wavelength of 810 ± 10 nm) in one, two or three repetitions and biocidal efficacy was assessed. To evaluate impact of laser irradiation on roughness, morphology and structure of the implant surface, optical profilometry, scanning electron microscopy and optical microscopy were used. Examination of the tested surfaces and saliva revealed the presence of Gram-positive and Gram-negative bacteria and one fungal species. In all patients, cultures from the endosseous part of the implant revealed the presence of the pathogenic and pyogenic bacterium Streptococcus constellatus. In 13 out of 20 samples laser-irradiated in duplicate and triplicate, all microorganisms were eliminated. The irradiation used did not cause any changes in the properties of the implant surface.

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Isolation and identification of potential fungal species for spent engine lubrication oil remediation in Peninsular Malaysia

Remediation Journal ◽

10.1002/rem.21564 ◽

2018 ◽

Vol 28 (3) ◽

pp. 91-95 ◽

Cited By ~ 3

Author(s):

Ong Ghim Hock ◽

Ho Cheng Cheng ◽

Vivian Lim Bi Fang ◽

Wong Yieng Yong ◽

Wong Ling Shing

Keyword(s):

Fungal Species ◽

Peninsular Malaysia ◽

Isolation And Identification ◽

Lubrication Oil

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Isolation and identification of Acanthamoeba genotypes and Naegleria spp. from the water samples of public swimming pools in Qazvin, Iran

Journal of Water and Health ◽

10.2166/wh.2019.074 ◽

2019 ◽

Vol 18 (2) ◽

pp. 244-251 ◽

Cited By ~ 1

Author(s):

Nastaran Paknejad ◽

Elham Hajialilo ◽

Mehrzad Saraei ◽

Amir Javadi

Keyword(s):

Water Samples ◽

Polymerase Chain Reaction Amplification ◽

Swimming Pools ◽

Isolation And Identification ◽

Highly Pathogenic ◽

Reaction Amplification ◽

Pcr Method ◽

Polymerase Chain ◽

Naegleria Lovaniensis ◽

Isolated Species

Abstract Free-living amoeba (FLA), including Acanthamoeba and Naegleria are facultative parasites in humans. The amoeba have widespread distribution in various water sources. The aim of this study was isolation and molecular identification of Acanthamoeba and Naegleria isolated from swimming pools and also hot and cold tub waters in Qazvin province. The samples (166 water samples) were cultured to isolate and identify positive specimens. PCR (polymerase chain reaction) amplification, sequencing and phylogenetic analysis were conducted to confirm the isolated species and genotypes of amoeba. According to morphological characterizations, 18.6% of specimens were identified as FLA, which in 71% were Acanthamoeba by PCR method. Molecular analysis revealed that 36.3%, 18.1% and 4.5% of Acanthamoeba specimens were identified as T3, T4 and T11 Acanthamoeba genotypes, respectively. Protacanthamoeba bohemica (27.2%) and Acanthamoeba sp. (4.5%) were found among the specimens. The results of osmo-tolerance and thermo-tolerance assays demonstrated that 50% of T3 and 25% of T4 genotypes of Acanthamoeba were highly pathogenic parasites. The molecular approach showed the presence of Naegleria lovaniensis (9%) in hot tub water of swimming pools. This study demonstrated that the swimming pools and hot tub water in Qazvin province were contaminated with Acanthamoeba and Naegleria species.

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Isolation and identification of common fungal spp. from commercial broiler feeds available in market of Sylhet District, Bangladesh

International Journal of Natural Sciences ◽

10.3329/ijns.v4i2.28603 ◽

2016 ◽

Vol 4 (2) ◽

pp. 38-41 ◽

Cited By ~ 1

Author(s):

MT Islam ◽

MK Hossain ◽

ATMM Elahi ◽

M Purkayastha ◽

MM Rahman

Keyword(s):

Fungal Species ◽

Natural Sciences ◽

Local Market ◽

Frequency Of Occurrence ◽

Isolation And Identification ◽

Fungal Isolates ◽

Commercial Broiler ◽

Rhizopus Sp ◽

Feed Samples ◽

Broiler Feed

The present study was designed to investigate the mycological contamination of commercial broiler feeds used in poultry establishments in sylhet, Bangladesh. The feed samples of commercial broiler feed (Starter, Grower and Finisher) were collected from the different areas of Sylhet district. A total of 189 commercial broiler feed samples where 63 Starter, 63 Grower and 63 Finisher were collected from the different areas of local market in Sylhet. The selected areas were Kadamtali, Shibjong, Khadim, Kamal Bazar, Dakshin Surma, Fenchugonj. From the feed samples analyzed for the presence of fungal agents, 144 (76.2%) were found positive for one or more fungal species. Fungal isolates were found among 36 (57%) of the 63 Starter feed samples, 45 (71.4%) of the 63 Grower feed samples and 63 (100%) of the 63 Finisher feed samples. The fungal agents isolated from Broiler Starter Feeds, Aspergillus spp. 51 (70.8%) has the highest frequency of occurrence, followed by Fuserium spp. 12 (16.7%) and least is Rhizopus sp. 9(12.5%). Similarly, in case of Broiler Grower Feeds, Aspergillus spp. 66 (68.8%) has the highest frequency of occurrence, followed by Fuserium spp. 18 (18.7%) and least is Rhizopus sp. 12(12.5%). In case of Broiler Finisher Feeds, Aspergillus spp. 90 (69.8%) has the highest rate of occurrence followed by Fuserium spp. 24 (18.6%) and least is Rhizopus sp. 15 (11.6%) respectively.International Journal of Natural Sciences (2014), 4(2) 38-41

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