scholarly journals Biodegradation of Anthracene Compound by Two Species of Filamentous Fungi

2018 ◽  
Vol 15 (1) ◽  
pp. 43-47
Author(s):  
Baghdad Science Journal
Keyword(s):  
Methylene Blue ◽  
Filamentous Fungi ◽  
Aspergillus Flavus ◽  
Biomass Production ◽  
Potato Dextrose Agar ◽  
High Ability ◽  
Isolation And Identification ◽  
Emericella Nidulans ◽  
Redox Indicator ◽  
Maximum Biomass Production

Isolation and identification fungi of Emericella nidulans and Aspergillus flavus from a pinkish and yellowish artificial clay, by using potato dextrose agar (PDA). Results revealed that E. nidulans was the best for degrading anthracene (92.3%) with maximum biomass production (3.7gm/l), compared to A. flavus with the rate of degradation (89%) and biomass production of (1.2gm/l), when methylene blue was used as redox indicator after incubating in a shaker incubator 120rpm at 30Co for 8days. Results indicated that E. nidulans has a high ability of anthracene degradation with the rate of (84%), while A. flavus showed the lower level with (77%) by using HPLC.

scholarly journals Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae)

2019 ◽  
Vol 37 (2) ◽  
pp. 232
Author(s):  
Agustin Indrawati ◽  
Damiana Rita Ekastuti ◽  
Erdina Pangestika ◽  
Reinilda Alwina
Keyword(s):  
Aspergillus Fumigatus ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Microscopic Observation ◽  
Potato Dextrose Agar ◽  
Aspergillus Versicolor ◽  
Wild Silkworm ◽  
Opportunistic Pathogenic

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum  and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus  flavus, Aspergillus fumigatus and Fusarium dimerum.

scholarly journals Isolation and identification of yeasts and filamentous fungi from yoghurts in Brazil

2001 ◽  
Vol 32 (2) ◽  
Author(s):  
Silvia Regina Moreira ◽  
Rosane Freitas Schwan ◽  
Eliana Pinheiro de Carvalho ◽  
Alan E. Wheals
Keyword(s):  
Filamentous Fungi ◽  
Isolation And Identification

scholarly journals Microbiological quality of commercially available poultry feeds sold in Bangladesh

2017 ◽  
Vol 3 (1) ◽  
pp. 52-60
Author(s):  
Nigar Sultana ◽  
Md Atiqul Haque ◽  
Md Mostafizer Rahman ◽  
Mir Rowshan Akter ◽  
Mst Deloara Begum ◽  
...  
Keyword(s):  
Public Health ◽  
Methylene Blue ◽  
Culture Media ◽  
Microbiological Quality ◽  
Control Measures ◽  
Nutrient Agar ◽  
Poultry Feed ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
E Coli

The study was conducted aiming at the isolation and identification of pathogens from poultry feed manufactured by four different brands namely A (broiler starter), B (broiler finisher), C (layer starter) and D (layer finisher) sold in retail stores of Rangpur city of Bangladesh. All these samples were collected from four randomly chosen outlets and analyzed by culturing in different culture media such as Nutrient broth (NB), Nutrient agar (NA), Salmonella-Shigella (SS) agar, Eosin methylene blue (EMB) agar, MacConkey agar, Triple sugar iron (TSI) agar slant, Motility, Indole, Urease (MIU) and Saboraud Dextrose agar (SDA) media. The bacterial agents were isolated and examined under light microscope for their gross morphological and conventional biochemical characteristics. The bacteriological analyses were done at the Microbiology Laboratory of Hajee Mohammad Danesh Science and Technology University, Dinajpur during the period of January to June, 2014. Total bacterial colonies of all the samples were counted separately according to the American Public Health Association, using nutrient agar medium for total viable count (TVC), Eosine methylene blue (EMB) agar media for total E. coli count (TEC) and Salmonella-Shigella agar for TSC (total salmonella count). Saboraud Dextrose agar (SDA) media was used for detection of fungus. The virulence effect of the organism present in feed were observed by inoculating the organism in poultry. Recorded result showed that average TVC of feed sample A, B, C and D were 5.45x106, 3.28x105, 5.14x106 and 4.53x105 CFU/gm (colony forming unit per gram) respectively. TEC of feed sample A, B, C and D were recorded 6.25x105, 8.26x103, 5.52x105 and 5.65x104 CFU/gm respectively. TSC of feed sample A, B, C and D were recorded 3.15x104, 2.68x103, 4.46x103 and 1.19x104 CFU/gm respectively. The highest TVC, TEC and TSC were found in broiler starter (feed sample A) and lowest TVC, TEC and TSC were found in broiler finisher (feed sample B). Fungal count was 1.85x105 CFU/ gm in layer finisher (feed sample D) could be as a result of their high pathogenecity as reported by researchers elsewhere. These organisms can cause several poultry and farm animal infections specially mycotoxicosis having public health significance to both human and poultry. The presence of high numbers of E. coli and Salmonella spp. in poultry feed were indicative of poor hygienic practices during manufacture, post process contamination and unsatisfactory transportation and reservation. Therefore reinforce the need for preventive control measures, hygienic handling and processing of feeds to reduce the risk of potential human health hazards.Asian J. Med. Biol. Res. March 2017, 3(1): 52-60

scholarly journals Efficacy of Caspofungin against Aspergillus flavus, Aspergillus terreus, and Aspergillus nidulans

2006 ◽  
Vol 50 (12) ◽  
pp. 4202-4205 ◽  
Author(s):  
J. C. Bowman ◽  
G. K. Abruzzo ◽  
A. M. Flattery ◽  
C. J. Gill ◽  
E. J. Hickey ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Amphotericin B ◽  
Filamentous Fungi ◽  
Aspergillus Flavus ◽  
Aspergillus Terreus ◽  
Potent Inhibitor ◽  
Murine Models ◽  
Glucan Synthase ◽  
Fungal Burden

ABSTRACT The echinocandin caspofungin is a potent inhibitor of the activity of 1,3-β-d-glucan synthase from Aspergillus flavus, Aspergillus terreus, and Aspergillus nidulans. In murine models of disseminated infection, caspofungin prolonged survival and reduced the kidney fungal burden. Caspofungin was at least as effective as amphotericin B against these filamentous fungi in vivo.

Studies on the mycoflora and its control on the seeds of some forest trees. I. Cedrus deodara

1983 ◽  
Vol 61 (1) ◽  
pp. 197-201
Author(s):  
R. K. Mittal
Keyword(s):  
Seed Germination ◽  
Aspergillus Flavus ◽  
Fusarium Moniliforme ◽  
Rhizopus Oryzae ◽  
Common Species ◽  
Fungal Species ◽  
Seedling Development ◽  
Potato Dextrose Agar ◽  
Forest Trees ◽  
Cedrus Deodara

By tests using standard moist blotters, potato–dextrose–agar plates, and moist sterilized sand, 26 fungal species belonging to 13 genera were isolated from seeds of Cedrus deodara Loud. Most of these fungi were present on seed surfaces and only nine caused deep-seated internal infections. Some of the more common species in different tests were Aspergillus flavus, A. luchuensis, Epicoccum purpurascens, Fusarium moniliforme, Pénicillium canadense, and Rhizopus oryzae. Aspergillus flavus, Penicillium canadense, and Rhizopus oryzae showed differences in pathogenicity to seed germination and seedling development in sterilized and unsterilized soils. Of the nine fungicides tested for the control of the seed-borne fungi, RH-2161, a liquid fungicide, was found to be the most effective. Dithane M-45 was also effective, whereas the remaining seven fungicides were less effective.

Tremorgenic indole metabolites and aflatoxins in sclerotia of Aspergillus flavus: an evolutionary perspective

1982 ◽  
Vol 60 (5) ◽  
pp. 525-528 ◽  
Author(s):  
Donald T. Wicklow ◽  
Richard J. Cole
Keyword(s):  
Aspergillus Flavus ◽  
Culture Medium ◽  
Cyclopiazonic Acid ◽  
Petri Dish ◽  
Potato Dextrose Agar ◽  
Evolutionary Perspective ◽  
Toxic Compounds ◽  
Defense Systems ◽  
Selective Forces ◽  
First Time

Isolates of Aspergillus flavus Link from both cool and warm latitudes were cultured on potato dextrose agar containing yeast extract to identify sclerotia-producing strains. Chloroform–MeOH extracts of sclerotia were analyzed for the presence of aflatoxins and major indole metabolites (e.g., cyclopiazonic acid, aflatrem, and dihydroxyaflavinine). Aflatoxin is reported from sclerotia of A. flavus for the first time. Cyclopiazonic acid was detected primarily in sclerotia of isolates from warmer latitudes. Aflatrem and dihydroxyaflavinine were detected in sclerotia from 85% of the strains examined. These metabolites are associated with the sclerotial stage of the life cycle, because neither were detected in extracts of the culture medium and mycelium of Petri dish cultures from which all the sclerotia were removed. Geographic variation and intrafungal allocation of these toxic compounds in A. flavus are examined from the evolutionary ecologist's perspective of selective forces shaping the chemical defense systems of fungi.

scholarly journals SEMI QUANTITATIVE RISK PROFILE OF AFLATOXIN B1 FROM AN ASPERGILLUS FLAVUS IN A DRIED SALTED FISH IN JAVA ISLAND RESELLERS

2018 ◽  
Vol 13 (2) ◽  
pp. 85
Author(s):  
Izhamil Hidayah
Keyword(s):  
Aspergillus Flavus ◽  
Aflatoxin B1 ◽  
Probabilistic Approach ◽  
Risk Profile ◽  
Risk Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Isolation And Identification ◽  
Plate Method ◽  
Salted Fish ◽  
Sampling Locations

This study aims to determine the probability of Aflatoxin B1 exposure from Aspergillus flavus in dried salted fish. The exposure of that may cause a health problems to consumers. The collecting of salted fish was conducted in several areas in Java Island, which were Tangerang, Bandung, Cirebon, Pelabuhan Ratu, Tegal, Cilacap, Banyuwangi and Tuban. Isolation and identification of Aspergillus flavus was conducted by using pour plate method on  Aspergillus Flavus Parasiticus Agar (AFPA)  media. Meanwhile, Measurement of Aflatoxin B1 (AFB1) content had been done by enzyme linked immunosorbent assay (ELISA). On the other hand, the occurence probability of Aflatoxin B1 risk from Aspergillus flavus was calculated by statistical probabilistic approach in @risk version 7.0 software with Monte Carlo simulation. The results of this study showed that in the consumption of salted fish for about 3.7 g/capita/day, it is probable that there are risk of 7.74 cfu/g A. flavus exposure and 0.7291 ppb aflatoxin B1 exposure in 1 g of salted fish that were taken from sampling locations.  This value is still catagorized as low risk level

scholarly journals Isolation and Identification of Fungi Associated with the Spoilage of Sweet Orange and Banana in Sokoto Metropolis

2020 ◽  
Author(s):  
Michael Ameh
Keyword(s):  
Citrus Sinensis ◽  
Room Temperature ◽  
Sweet Orange ◽  
Potato Dextrose Agar ◽  
Test Results ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Musa Sapientum ◽  
Identification Of Fungi ◽  
Pure Cultures

Mycological studies on Fungi in apparently diseased Sweet Orange ( Citrus sinensis) and Banana ( Musa sapientum) sampled from various points in Kara market in Sokoto Metropolis was carried out between August and September. The samples were surfaced sterilised with ethanol and the homogenates were cultured on Potato Dextrose Agar (PDA) and incubated aerobically at room temperature for 7 days at 30C. The pure cultures obtained were identified morphologically and microscopically. The investigation revealed that the samples were infected with several fungi species. The most predominant Fungi isolated from Sweet Orange were Cladosporium spp (40%), Fusarium spp (30%), Alternaria spp ( 20%), and Chrysonilia spp (10%) while the most predominant Fungi isolated from Banana were Fusarium spp (50%), Mucor spp ( 30%) and Rhizopus spp (20%). The pathogenecity test results show that Cladosporium spp and Fusarium spp were the most active in sweet orange with rot length of 74 mm and 70 mm respectively and the least active fungi were Alternaria spp and Chrysonilia spp with rot lengths of 52 mm and 48 mm respectively. Furthermore, Fusarium spp and Mucor spp were the most active in banana with rot lengths of 84 mm and 75 mm respectively and the least active fungus was Rhizopus spp with rot lengths of 54 mm.

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