Studies on the mycoflora and its control on the seeds of some forest trees. I. Cedrus deodara

1983 ◽  
Vol 61 (1) ◽  
pp. 197-201
Author(s):  
R. K. Mittal

By tests using standard moist blotters, potato–dextrose–agar plates, and moist sterilized sand, 26 fungal species belonging to 13 genera were isolated from seeds of Cedrus deodara Loud. Most of these fungi were present on seed surfaces and only nine caused deep-seated internal infections. Some of the more common species in different tests were Aspergillus flavus, A. luchuensis, Epicoccum purpurascens, Fusarium moniliforme, Pénicillium canadense, and Rhizopus oryzae. Aspergillus flavus, Penicillium canadense, and Rhizopus oryzae showed differences in pathogenicity to seed germination and seedling development in sterilized and unsterilized soils. Of the nine fungicides tested for the control of the seed-borne fungi, RH-2161, a liquid fungicide, was found to be the most effective. Dithane M-45 was also effective, whereas the remaining seven fungicides were less effective.

2012 ◽  
Vol 10 (1) ◽  
pp. 46-50 ◽  
Author(s):  
F. Yeasmin ◽  
M. Ashrafuzzaman ◽  
I. Hossain

Seeds of rice were treated with Garlic (Allium sativum) clove extract @ 1:0, 1:1, 1:2 dilutions in water, allamanda (Allamanda cathartica) leaf extract @ 1:1, 1:2, 1:3 dilutions in water and Provax-200 @ 0.3% for controlling seed borne fungi, where the seed samples of three rice varieties viz. Katharee, Gutee Aus and Kalijira were collected from farmer’s storages of Bangladesh. The seed germination under control ranged from 64 to 77%, where treatments resulted up to 100% germination. The identified seed borne fungi of rice were Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum, F. moniliforme, Nigrospora oryzae, Aspergillus flavus, Aspergillus niger and Penicillium sp., where prevalence of Bipolaris oryzae (7.5%) and Fusarium moniliforme (8.3%) were the maximum. All the treatments significantly reduced the seed borne fungi up to 100% over the control, where Provax was found best and was statically similar to garlic (1:1) extract against seed borne pathogen of rice.DOI: http://dx.doi.org/10.3329/agric.v10i1.11064The Agriculturists 2012; 10(1): 46-50


1994 ◽  
Vol 57 (3) ◽  
pp. 256-258 ◽  
Author(s):  
M. L. ABARCA ◽  
M. R. BRAGULAT ◽  
G. CASTELLÁ ◽  
F. J. CABAÑES

The mycoflora of 69 samples of animal mixed feeds were studied. Fungal counts ranged from 102 to 108 CFU/g, the lowest counts corresponding to the samples of rabbit feeds. Seventy-one fungal species belonging to 26 genera were identified. The pre- dominant species were Aspergillus flavus, Fusarium moniliforme, and Penicillium chrysogenum. Thirty-six strains of A. flavus and one strain of A. parasiticus were screened for aflatoxin production in yeast extract-sucrose medium. The final pH, weight of mycelium, and production of aflatoxins were determined after 14 days of incubation. Five strains (13.5%) were aflatoxigenic. No statistical differences were observed in mycelial dry weights and final pH between aflatoxin-producing strains and nonaflatoxigenic strains.


1970 ◽  
Vol 6 (2) ◽  
pp. 245-251 ◽  
Author(s):  
MAM Ara ◽  
ML Khatun ◽  
M Ashrafuzzaman

Five varieties of diseased onion samples, four local (Taherpuri, Faridpuri, Kalashnagari, and Zitka) and one Indian (Pusa Red) were collected from different markets of Mymensingh, to isolate and identify the associated fungi. Five different fungal species belonging to three genera were repeatedly isolated. Healthy looking and diseased onions were kept at room temperature. The population of associated fungi increased proportionately with the duration of storage period. The isolated fungi were Aspergillus niger, Aspergillus flavus, Penicillium spp., Fusarium oxysporum and Fusarium moniliforme. Healthy onion bulbs were inoculated (with injury and without injury) with these fungi. Individual fungi produced distinguishable symptom of disease in incubation chamber at 22-23°C temperature. After 30 days of inoculation, bulbs showed comparatively low amount of disease development than those at 60 and 90 days. Maximum disease development was found in the variety Pusa Red (with injury) and it was minimum in Zitka variety (without injury). Keywords: Fungi; Onion; Disease development DOI: 10.3329/jbau.v6i2.4818 J. Bangladesh Agril. Univ. 6(2): 245-251, 2008


2013 ◽  
pp. 111-119 ◽  
Author(s):  
Vesna Krnjaja ◽  
Jelena Levic ◽  
Slavica Stankovic ◽  
Tanja Petrovic ◽  
Milos Lukic

Incidence of toxigenic fungi (molds) and concentration of mycotoxin aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZON) and fumonisin (FB1) were studied in the maize grains collected immediately after harvesting in 2012. A total of 29 maize samples were analyzed and the highest incidence was determined for fungal species of Rhizopus (56.41%), Aspergillus (43.66%) and Fusarium (14.97%) genera. Significantly lower incidence was obtained for species of genus Penicillium (3.31%), and especially for species of genera Acremonium (1.38%), Alternaria (0.75%) and Cladosporium (0.14%). Among toxigenic fungi Aspergillus flavus (36.69%) was the most common species of As?pergillus genus, whereas the Fusarium verticillioides with 14.69% of incidence was the predominant species of Fusarium genus. In all studied maize samples, the presence of AFB1, ZON and FB1 mycotoxins was established, except for DON which was established in 75.86% samples. AFB1 was detected in average concentration of 13.95 ?g kg-1 for 44.83% of samples, and average concentration higher than 40 ?g kg-1 for 55.17% of samples. The average concentrations of DON which was detected was 235 ?g kg-1, while it was 98.38 ?g kg-1 and 3590 ?g kg-1 for the presence of ZON and FB1, respectively. Moderate positive correla?tion was obtained between concentrations of AFB1 and FB1 (r=0.35), while weak positive correlation was established between concentrations ZON and DON (r=0.02).


Helia ◽  
2001 ◽  
Vol 24 (34) ◽  
pp. 77-82 ◽  
Author(s):  
Rauf Bhutta ◽  
M.H. Rahber Bhatti ◽  
Ahmad Iftikhar

SUMMARYAll four seed diffusates used for treatment of sunflower seeds, Azadirachtaindica, Capsicum annuum, Coriandrum sativum and Eugenia jambulana, reduced the populations of seed-borne fungi: Alternaria alternata, Drechslera tetramera, Emericellopsis terricola, Fusarium moniliforme, F.semitectum, Macrophomina phaseolina and Phoma oleracea. Of four seed diffusates, those from A.indica and C.sativum controlled the fungal populations almost 100%. Seed germination was increased in seed samples of both sunflower cultivars under study, HO-1 and NK-212. The obtained results indicate that seed diffusates could substitute costly chemicals for safe control of seed-borne diseases, protecting at the same time the environment from chemical pollution.


2021 ◽  
Vol 22 (9) ◽  
pp. 4738
Author(s):  
Hye-Yeon Seok ◽  
Hyungjoon Bae ◽  
Taehyoung Kim ◽  
Syed Muhammad Muntazir Mehdi ◽  
Linh Vu Nguyen ◽  
...  

Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, the functions and molecular aspects of many non-tandem CCCH zinc finger (non-TZF) proteins remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein and belongs to the ZFWD subfamily harboring a CCCH zinc finger motif and a WD40 domain. In this study, we characterized the biological and molecular functions of AtC3H59, which is subcellularly localized in the nucleus. The seeds of AtC3H59-overexpressing transgenic plants (OXs) germinated faster than those of wild type (WT), whereas atc3h59 mutant seeds germinated slower than WT seeds. AtC3H59 OX seedlings were larger and heavier than WT seedlings, whereas atc3h59 mutant seedlings were smaller and lighter than WT seedlings. Moreover, AtC3H59 OX seedlings had longer primary root length than WT seedlings, whereas atc3h59 mutant seedlings had shorter primary root length than WT seedlings, owing to altered cell division activity in the root meristem. During seed development, AtC3H59 OXs formed larger and heavier seeds than WT. Using yeast two-hybrid screening, we isolated Desi1, a PPPDE family protein, as an interacting partner of AtC3H59. AtC3H59 and Desi1 interacted via their WD40 domain and C-terminal region, respectively, in the nucleus. Taken together, our results indicate that AtC3H59 has pleiotropic effects on seed germination, seedling development, and seed development, and interacts with Desi1 in the nucleus via its entire WD40 domain. To our knowledge, this is the first report to describe the biological functions of the ZFWD protein and Desi1 in Arabidopsis.


Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.


2011 ◽  
Vol 98 (10) ◽  
pp. 1613-1622 ◽  
Author(s):  
Clesnan Mendes-Rodrigues ◽  
Marli A. Ranal ◽  
Paulo E. Oliveira

AoB Plants ◽  
2015 ◽  
Vol 7 ◽  
Author(s):  
Risolandia Bezerra de Melo ◽  
Augusto César Franco ◽  
Clovis Oliveira Silva ◽  
Maria Teresa Fernandez Piedade ◽  
Cristiane Silva Ferreira

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