scholarly journals REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES

2017 ◽  
Vol 11 (2) ◽  
Author(s):  
Gholib Gholib ◽  
Muhammad Agil ◽  
Bambang Purwantara ◽  
Michael Heistermann ◽  
Antje Engelhardt
Keyword(s):  
Ambient Temperature ◽  
Control Group ◽  
Short Term ◽  
Freeze Thaw ◽  
North Sulawesi ◽  
Macaca Nigra ◽  
Significant Difference ◽  
Short Term Storage ◽  
The Stability ◽  
Term Storage

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P<0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P>0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.

Influence of temperature and short-term storage on the stability of norepinephrine in plasma samples

1994 ◽  
Vol 348 (10) ◽  
pp. 696-698 ◽  
Author(s):  
Manfred Volz ◽  
Gisela Gottschalk ◽  
Hussein Husseini ◽  
Veselin Mitrovic ◽  
Martin Schlepper
Keyword(s):  
Plasma Samples ◽  
Short Term ◽  
Influence Of Temperature ◽  
Short Term Storage ◽  
The Stability ◽  
Term Storage

scholarly journals Effect of Different Media on Short Term Storage of Leaping Mullet (Liza saliens (Risso, 1810)) Sperm

2020 ◽  
Vol 8 (4) ◽  
pp. 996-1000
Author(s):  
Serhat Engin ◽  
Muammer Kürşat Fırat ◽  
Osman Özden ◽  
Cüneyt Suzer ◽  
Müge Hekimoğlu ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Control Group ◽  
Natural Seawater ◽  
Short Term ◽  
Liza Saliens ◽  
Seawater Salinity ◽  
Significant Difference ◽  
Term Storage ◽  
Quality Variation ◽  
Motility Rate

The present study aimed to evaluate the activation characteristics of the leaping mullet spermatozoa and diluted with four different media (D1, D2, D3, D4) for 250 h at 4±2°C. The semen was collected from nine wild fish in Homa Lagoon, İzmir, Turkey in July, during reproductive season. In each trial (n = 9), semen samples were collected and mixed to reduce sperm quality variation to obtain the required gametes for the experiment. The semen was activated with natural seawater (salinity of 34, pH 7.9 and 4±2°C) to determine: motility time, motility rate and viability of sperm. Dilution with inactivation media 1:3 increased motility time of diluted semen than control group. At the end of the experiment, no significant difference was found for the duration of survival in the D2 and D4 groups. The highest survival rate was found in D2 group.

scholarly journals Design of a refrigerated complex for short-term storage of tropical fruits with a solar energy plant

2021 ◽  
Vol 3 (3(59)) ◽  
pp. 50-57
Author(s):  
Larisa Morozyuk ◽  
Viktoriia Sokolovska-Yefymenko ◽  
Yaroslav Petushkov ◽  
Maksym Sharaiev ◽  
Sergii Psarov
Keyword(s):  
Heat Treatment ◽  
Ambient Temperature ◽  
Thermal Load ◽  
Power Plants ◽  
Thermal Power ◽  
Thermal Calculation ◽  
Tropical Fruits ◽  
Short Term ◽  
Short Term Storage ◽  
Term Storage

The object of research is a refrigerated complex for short-term storage of tropical fruits in conditions of significant seasonal and daily fluctuations in ambient temperature, that typical for regions with a tropical climate. One of the problems is that the complexes are autonomous small firms for the year-round processing and storage of tropical fruits, located far from the central electric networks. In the presence of solar radiation, the complexes receive energy from small solar power plants. Such complexes are called «trigeneration system». In the course of the study, data on modes were used low temperature heat treatment and preservation of various tropical fruits, ripening times and climatic conditions of Tunisia. It has been established that citrus fruits are stored in chambers with high temperature, olives are frozen and stored for a short time before processing. The total amount of heat entering the citrus chambers is determined by changes in the ambient temperature. The thermal load of the olives chamber is determined by the heat treatment time. It was found that the cargo capacity of chambers with different temperatures differs six times. The thermal load of the olive storage chambers is only four times less. This is due to the peculiarities of the building structure of the complex, technological processes of cooling and freezing. Based on the thermal calculation, the cooling of the chambers is provided by a two-stage booster refrigeration machine with CO2 refrigerant in a transcritical cycle. To ensure the operation of the complex, a solar photoelectric converter is designed. This ensures the environmental safety of the complex and the possibility of obtaining energy savings by regulating the thermal power of the compressors with frequency converters, depending on the ambient temperature. The designed complex can be offered to a private investor for practical implementation.

scholarly journals Changes of wheat flour properties during short term storage

2011 ◽  
Vol 20 (No. 4) ◽  
pp. 125-130 ◽  
Author(s):  
M. HRUŠKOVÁ ◽  
D. MACHOVÁ
Keyword(s):  
Ambient Temperature ◽  
Wheat Flour ◽  
Viscoelastic Properties ◽  
Falling Number ◽  
Short Term ◽  
Two Samples ◽  
Flour Properties ◽  
Short Term Storage ◽  
Temperature And Humidity ◽  
Term Storage

Two samples of commercial wheat flour from the last year&rsquo;s harvest were stored for three months (in the period from November to April) under different conditions. The ambient temperature and humidity varied during the storage in the dependence on the year season. Certain analytical characteristics (moisture, wet gluten and its extensibility, acidity and falling number) and alveograph behaviour of flour were determined at regular intervals. Flour moisture, acidity, and falling number changed with the time of storage but no explicit influence of the storehouse conditions and the initial flour properties was proved. Viscoelastic properties of weaker flour samples changed during storage more markedly than those of stronger flours in the sense of a significant improvement of their quality. &nbsp;

Effects of Freeze-Thaw Cycles and Assessment of Short-Term Storage Stability on Serum Iron, Ferritin, and Transferrin

2021 ◽  
Vol 67 (02/2021) ◽  
Author(s):  
Rogério Klat ◽  
Carolina Stein ◽  
Mariane Santos ◽  
Rafael Moresco
Keyword(s):  
Serum Iron ◽  
Storage Stability ◽  
Short Term ◽  
Freeze Thaw ◽  
Short Term Storage ◽  
Term Storage
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