scholarly journals Effect of Ultrashort Wave on Joint Dysfunction and Muscle Atrophy in a Rabbit Model of Extending Knee Joint Contracture: Enhanced Expression of MyoD

2019 ◽  
Author(s):  
Feng Wang ◽  
Yun Zhou ◽  
Quan Bing Zhang ◽  
A Ying Liu ◽  
Hua Zhang Zhong ◽  
...  
Keyword(s):  
Knee Joint ◽  
Muscle Atrophy ◽  
Rabbit Model ◽  
Rectus Femoris ◽  
Joint Contracture ◽  
Passive State ◽  
Control Group ◽  
Wave Treatment ◽  
Protein Levels ◽  
Ultrashort Wave

Abstract Background As a common clinical disease, the incidence of joint contracture which is characterized by the reduction of range of motion (ROM) in the active or passive state of the joint has increased in recent years. This study was to investigate the effects of ultrashort wave on joint dysfunction and muscle atrophy in a rabbit model of extending knee joint contracture and its mechanism. Methods 35 rabbits underwent unilateral immobilization of a knee joint at full extension to cause joint contracture, and 5 rabbits were used for the control group. After 8 weeks immobilization, 35 rabbits were randomly divided into the following seven groups: I-8, R-1, R-2, R-4, T-1, T-2, and T-4. In the Group R-1, R-2 and R-4, the rabbits were experienced one, two, and four weeks self-recovery. In the Group T-1, T-2, and T-4, the rabbits were experienced one, two, and four weeks ultrashort wave treatment. The effect of self-recovery and ultrashort wave treatment on joint dysfunction and muscle atrophy was assessed by measuring the degree of total and myogenic contracture, evaluating the cross-sectional area (CSA) of rectus femoris and assessing the protein levels for MyoD. Results A tendency toward reduced the degree of total and myogenic contracture was observed after self-recovery and ultrashort wave treatment. A tendency toward increased the CSA of rectus femoris and the protein levels for MyoD was observed after self-recovery and ultrashort wave treatment. The ultrashort wave treatment led a better efficacy than self-recovery against the total and the myogenic contracture, the CSA and the protein levels for MyoD of rectus femoris. Conclusions Ultrashort wave ameliorates joint dysfunction and muscle atrophy via upregulating the expression of MyoD protein in a rabbit model of extending knee joint contracture.

scholarly journals Preventive effect and possible mechanisms of ultrashort wave diathermy on myogenic contracture in a rabbit model

2021 ◽  
Vol 104 (4) ◽  
pp. 003685042110549
Author(s):  
Qi-Yu Xu ◽  
Quan-Bing Zhang ◽  
Yun Zhou ◽  
A-Ying Liu ◽  
Feng Wang
Keyword(s):  
Muscle Atrophy ◽  
Myogenic Differentiation ◽  
Rabbit Model ◽  
Rectus Femoris ◽  
Control Group ◽  
Preventive Effect ◽  
Cross Sectional ◽  
Protein Levels ◽  
Group I ◽  
Ultrashort Wave

The purpose of this study was to determine the preventive effect of ultrashort wave diathermy on immobilization-induced myogenic contracture and to explore its underlying mechanisms. Forty-two rabbits were randomly assigned into control (Group C), immobilization (Group I, which was further divided into one week, Group I-1; two weeks, Group I-2; and four weeks, Group I-4, subgroups by the length of immobilization) and ultrashort wave prevention (Group U, which was further divided into one week, Group U-1; two weeks, Group U-2; and four weeks, Group U-4, by time of treatment) groups. Intervention effects were assessed by evaluating rectus femoris cross-sectional area (CSA), knee range of motion, and the protein levels for myogenic differentiation (MyoD) and muscle atrophy F-box (MAFbx-1) in the rectus femoris. Compared with those of Group C, in Groups I and U, total contracture, myogenic contracture, MyoD and MAFbx-1 levels were significantly elevated, and CSA was significantly smaller ( p < 0.05). Compared with those of Group I at each time point, MyoD levels were significantly elevated, MAFbx-1 levels were significantly lower, CSA was significantly larger, and myogenic contracture was significantly alleviated in Group U ( p < 0.05). In the early stages of contracture, ultrashort wave diathermy reduces muscle atrophy and delays the process of myogenic contracture during joint immobilization; the mechanism of this may be explained as increased expression of MyoD triggered by suppression of the MAFbx-1-mediated ubiquitin-proteasome pathway.

scholarly journals Rabbit Model of Extending Knee Joint Contracture: Progression of Joint Motion Restriction and Subsequent Joint Capsule Changes after Immobilization

2018 ◽  
Vol 33 (01) ◽  
pp. 015-021 ◽  
Author(s):  
Yun Zhou ◽  
Quan Bing Zhang ◽  
Hua Zhang Zhong ◽  
Yi Liu ◽  
Jun Li ◽  
...  
Keyword(s):  
Knee Joint ◽  
Range Of Motion ◽  
Rabbit Model ◽  
Joint Capsule ◽  
Joint Contracture ◽  
Control Group ◽  
Mrna Levels ◽  
Joint Range Of Motion ◽  
Joint Range ◽  
Tgf Β1

AbstractThis study aimed to develop a rabbit model of knee contracture in extension and investigate the natural history of motion loss and time-dependent changes in the joint capsule after immobilization. We immobilized the unilateral knee joints of 32 rabbits by maintaining the knee joint in a plaster cast at full extension. Eight rabbits were euthanized at 2, 4, 6, and 8 weeks after casting, respectively, and the lower extremities were disarticulated at the hip joint. Eight control group rabbits that did not undergo immobilization were also examined. We assessed the progression of joint contracture by measuring the joint range of motion, evaluating the histologic alteration of the capsule, and assessing the mRNA levels of transforming growth factor β1 (TGF-β1) in the anterior and posterior joint capsules. After 2 weeks of joint immobilization, the knee joint range of motion was limited, the synovial membrane of the suprapatellar and posterior joint capsules was thickened, the collagen deposition was increased, and the mRNA levels of TGF-β1 were elevated in the anterior and posterior joint capsules. These changes progressed rapidly until 6 weeks of immobilization and may advance slowly after 6 weeks. Joint contracture developed at the early stage of immobilization and progressed over time. The changes in the anterior and posterior joint capsules after joint immobilization may contribute to the limitation in flexion. The elevated mRNA expression of TGF-β1 may be related to joint capsule fibrosis and may be one of the causes of joint contracture.

scholarly journals Electrical Stimulation Attenuates Disuse Muscular Atrophy by Modulating Endoplasmic Reticulum Stress-induced Parkin-dependent Mitophagy

2022 ◽  
Author(s):  
Quan-Bing Zhang ◽  
A-Ying Liu ◽  
Qiao-Zhou Fang ◽  
Hai-Yang Zhang ◽  
Feng Wang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Electrical Stimulation ◽  
Endoplasmic Reticulum Stress ◽  
Muscle Atrophy ◽  
Muscular Atrophy ◽  
Rabbit Model ◽  
Rectus Femoris ◽  
Protein Levels ◽  
Elevated Expression

Abstract Background: The aim of this study was to investigate the therapeutic effect of electrical stimulation on disuse muscular atrophy in a rabbit model of knee joint contracture and explore the role of endoplasmic reticulum stress-induced Parkin-dependent mitophagy in this process.Methods: Two sub-experiments were carried out successively in our study. In the first sub-experiment, 24 rabbits were divided into four groups on average based on the immobilization time: Ctrl 1, I-2, I-4, and I-6 groups. In the second sub-experiment, 24 rabbits were also divided into four groups on average in accordance with the process mode: Ctrl2, ES, NR, and EST groups. To test the time-dependent changes of the rectus femoris muscles after immobilization in rabbits, and to evaluate the effect of electrical stimulation on the atrophic rectus femoris muscles, the wet weights of rectus femoris muscles were assessed in this study, along with the protein levels of atrogin-1, p-PERK, Parkin and COXIV.Results: The wet weights of rectus femoris muscles, the protein levels of atrogin-1, p-PERK and Parkin increased after immobilization. It was also revealed that the protein levels of COXIV decreased after immobilization. Electrical stimulation was effective against muscle atrophy, the elevated expression of atrogin-1, p-PERK, Parkin, and the decreased expression of COXIV.Conclusions: Immobilization of unilateral lower limb could induce rectus femoris muscle atrophy, endoplasmic reticulum stress and Parkin mediated mitophagy. Endoplasmic reticulum stress-induced Parkin-dependent mitophagy may be one of the mechanisms by which electrical stimulation can play a significant role.

scholarly journals Effect of Glutamine Supplement Administration on the Reduction of Muscular Fatigue Post-Eccentric Exercise

2021 ◽  
Vol 55 (4) ◽  
pp. 275
Author(s):  
Afif Rusdiawan ◽  
Taufikkurrachman Taufikkurrachman
Keyword(s):  
Treatment Group ◽  
Muscle Strength ◽  
Knee Joint ◽  
Glutamine Supplementation ◽  
Control Group ◽  
Protein Levels ◽  
Drop Jumps ◽  
Post Test ◽  
The Mean ◽  
Leg Muscle

Eccentric activity can cause pain due to muscle damage and fatigue. Tired and damaged muscular state will degrade athletes' performance during a competition. Glutamine supplements are known to maintain plasma protein levels and can also improve muscle weakness due to inflammation that occurs after eccentric activity. The purpose of this study was to prove the effect of glutamine supplementation on the reduction of muscle fatigue after eccentric activity based on muscle strength and joint ROM. The subjects of this study were students of PJKR, IKIP Budi Utomo, who were randomly divided into 2 groups, the control group (K1) and treatment group (K2), each consisting of 22 respondents. The design of this research is randomized grsoup pre-test and post-test design. The eccentric activity performed was drop jumps on the bench with a height of 0.5 meters. Glutamine was administered orally after eccentric activity with a dose of 0.4 grams/kg BW in 450 ml water mineral 3 times (3 days). Measurement of Leg muscle strength was performed using Back and Leg Dynamometer by pulling with all the strength using leg muscles, whereas ROM measurements were measured using a goniometer performed on the knee joint in a passive way. The result of measurement obtained the mean of muscle strength of control group 63.7 ± 17.0 kg and treatment group 81.1 ± 17.0 kg at 72 hours. While result of ROM measurement obtained the mean of control group 124.3 ± 3.2 degrees and treatment group  131.7 ± 4.1 degrees at 72 hours. The analysis showed that glutamine administration in treatment group after eccentric activity increased leg muscle strength with p=0.000, and also increased knee joint ROM with p=0.000 at 72 hours after eccentric activity. In conclusion, glutamine administered with a dose of 0.4 gram/kg BW/day can increase leg muscle strength and knee joint ROM at 72 hours after eccentric activity.

A computer-controlled contracture correction device with low-load and continuous torque: An animal experiment and prototype design for clinical use

2007 ◽  
Vol 31 (2) ◽  
pp. 121-132
Author(s):  
M. Akai ◽  
M. Usuba ◽  
H. Sekiguchi ◽  
B. Hong ◽  
K. Iwashita ◽  
...  
Keyword(s):  
Knee Joint ◽  
Animal Experiment ◽  
Tissue Response ◽  
Joint Contracture ◽  
Fixation Method ◽  
Control Group ◽  
Clinical Use ◽  
Joint Movement ◽  
Low Load ◽  
Computer Controlled

The purpose of this study was to clarify the relationship between mechanical stress and tissue response of the contracted knee joint in rats and to propose a new design of contracture correction device for clinical use. Wistar rats were operated on to immobilize their knee joints with a procedure causing periarticular bleeding and were kept in flexed position for 40 days. At day 40, the immobilizing wire was removed, and after day 43, the contracted knee joint had been treated with tunable corrective devices secured by an external fixation method to the rear limb. These devices consisted of four types of motor-driving system which provided several different low-load and continuous stretch torques. Measuring the angle of maximum knee extension, its effectiveness was assessed comparing with a lower load and control group of natural recovery course. The device also had a cyclic joint movement within the acquired range of motion and an oval cam mechanism producing a small distraction force to the joint along its long axis. The results showed that an appropriate range of low-load continuous torque was more effective to correct joint contracture. On the basis of the animal experiment, a new computer-controlled, gas-driven contracture correction device was developed for clinical trial. It was concluded that mechanical application in a condition with low and continuous torque is a useful treatment for fixed joint contracture.

scholarly journals Radial Extracorporeal Shock Wave Reduce Myogenic Contracture and Muscle Atrophy via inhibiting NF-κB/HIF-1α Signaling Pathway in Rabbit

2021 ◽  
Author(s):  
Feng Wang ◽  
Wen Li ◽  
Yun Zhou ◽  
Peng Peng Huang
Keyword(s):  
Shock Wave ◽  
Knee Joint ◽  
Signaling Pathway ◽  
Muscle Atrophy ◽  
Biological Effects ◽  
Left Knee ◽  
Myogenic Regulatory Factors ◽  
Protein Levels ◽  
Extracorporeal Shock Wave ◽  
Time Point

Abstract BackgroundRecent studies have shown that radial extracorporeal shock wave therapy (rESWT) modulates viability and gene expression of human skeletal muscle cells in vitro. However, the underlying biological effects and mechanisms of rESWT on muscle atrophy and myogenic contracture in animal mode have not yet been investigated.ObjectiveTo investigate the underlying biological effects and mechanisms of rESWT on myogenic contracture and muscle atrophy in a rabbit model of extending knee joint contracture.MethodsForty rabbits were randomly divided into eight groups. In group Control, the left knee joint was not fixed. In group I-4w, the left knee joint was only fixed for four weeks. In groups SR-1w, SR-2w, and SR-4w, the left knee joint was fixed for four weeks before the rabbits underwent one, two, and four weeks of self-recovery, respectively. In groups rESWT-1w, rESWT-2w, and rESWT-4w, the left knee joint was fixed for four weeks before the rabbits underwent one, two, and four weeks of rESWT, respectively. The degree of myogenic contracture was measured, the cross-sectional area and key protein levels for NF-κB/HIF-1α signaling pathway and myogenic regulatory factors of the rectus femoris were evaluated.ResultsDuring the recovery period, biological findings in this study showed that the levels of myogenic contracture and muscle atrophy were milder in group rESWT by compared with group SR at the same time point. Molecular biological analysis in the present study showed that MyoD protein levels in the group rESWT was significantly higher than those in the group SR, and importantly, phospho-NF-κB p65 and HIF-1α protein levels in the group rESWT were significantly lower than those in the group SR at the same time point.ConclusionsOur study demonstrates that rESWT has the potential to reduce myogenic contracture and muscle atrophy after long-term immobilization in animal model. It is a possible mechanism that changing the low oxygen environment in skeletal muscle through rESWT may inhibit activation of NF-κB/HIF-1α signaling pathway, thereby enhancing the expression of myogenic regulatory factors.

scholarly journals Low-frequency Electrical Stimulation Alleviates Immobilization-evoked Disuse Muscle Atrophy via Repressing Autophagy in Skeletal Muscle of Rabbits

2021 ◽  
Author(s):  
A-Ying Liu ◽  
Quan-Bing Zhang ◽  
Hua-Long Zhu ◽  
Yong-Wei Xiong ◽  
Feng Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Knee Joint ◽  
Muscle Atrophy ◽  
Rabbit Model ◽  
Low Frequency ◽  
Cross Sectional ◽  
Disuse Muscle Atrophy ◽  
Joint Range ◽  
Time Point

Abstract Objective: The present study was to investigate the effect of low-frequency electrical stimulation on disuse muscle atrophy and its mechanism in a rabbit model of extending knee joint contracture.Methods: This study designed two experiments. In the time-point experiment, 24 rabbits were randomly divided into Control 1(Ctrl1), immobilization for 2 weeks (I-2), I-4, and I-6 groups. In the intervention experiment, 24 rabbits were also randomly divided into Control 2 (Ctrl2), electrical stimulation (ES), natural recovery (NR) and electrical stimulation treatment (EST) groups. All intervention effects were assessed by evaluating the knee joint range of motion (ROM), cross-sectional area (CSA) of muscle and the expression of autophagy-related proteins.Results: Time-point experiment showed that immobilization reduced knee ROM, muscle CSA, and activated autophagy in skeletal muscle. Levels of four autophagic proteins including p-mTOR, Atg7, p62 and LC3B-II, were significantly elevated in the skeletal muscle of I-4 group. The intervention experiment further presented that LFES significantly improved the immobilization-induced ROM and CSA reduction. Additionally, LFES significantly reversed autophagy activation of skeletal muscle caused by immobilization.Conclusions: Low-frequency electrical stimulation alleviates immobilization-evoked disuse muscle atrophy maybe via inhibiting autophagy in skeletal muscle of rabbits.

scholarly journals Effect of Glutamine Supplement Administration on the Reduction of Muscular Fatigue Post-Eccentric Exercise

2020 ◽  
Vol 55 (4) ◽  
pp. 275
Author(s):  
Afif Rusdiawan ◽  
Taufikkurrachman Taufikkurrachman
Keyword(s):  
Treatment Group ◽  
Muscle Strength ◽  
Knee Joint ◽  
Glutamine Supplementation ◽  
Control Group ◽  
Protein Levels ◽  
Drop Jumps ◽  
Post Test ◽  
The Mean ◽  
Leg Muscle

Eccentric activity can cause pain due to muscle damage and fatigue. Tired and damaged muscular state will degrade athletes' performance during a competition. Glutamine supplements are known to maintain plasma protein levels and can also improve muscle weakness due to inflammation that occurs after eccentric activity. The purpose of this study was to prove the effect of glutamine supplementation on the reduction of muscle fatigue after eccentric activity based on muscle strength and joint ROM. The subjects of this study were students of PJKR, IKIP Budi Utomo, who were randomly divided into 2 groups, the control group (K1) and treatment group (K2), each consisting of 22 respondents. The design of this research is randomized grsoup pre-test and post-test design. The eccentric activity performed was drop jumps on the bench with a height of 0.5 meters. Glutamine was administered orally after eccentric activity with a dose of 0.4 grams/kg BW in 450 ml water mineral 3 times (3 days). Measurement of Leg muscle strength was performed using Back and Leg Dynamometer by pulling with all the strength using leg muscles, whereas ROM measurements were measured using a goniometer performed on the knee joint in a passive way. The result of measurement obtained the mean of muscle strength of control group 63.7 ± 17.0 kg and treatment group 81.1 ± 17.0 kg at 72 hours. While result of ROM measurement obtained the mean of control group 124.3 ± 3.2 degrees and treatment group  131.7 ± 4.1 degrees at 72 hours. The analysis showed that glutamine administration in treatment group after eccentric activity increased leg muscle strength with p=0.000, and also increased knee joint ROM with p=0.000 at 72 hours after eccentric activity. In conclusion, glutamine administered with a dose of 0.4 gram/kg BW/day can increase leg muscle strength and knee joint ROM at 72 hours after eccentric activity.

scholarly journals Hepatic Overexpression of GRP94 in a Rabbit Model of Parenteral Nutrition-Associated Liver Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xueping Zhu ◽  
Xiaomin Zhang ◽  
Lingling Yu ◽  
Yumin Xu ◽  
Xing Feng ◽  
...  
Keyword(s):  
Liver Disease ◽  
Parenteral Nutrition ◽  
Biochemical Parameters ◽  
Regulatory Protein ◽  
Rabbit Model ◽  
Control Group ◽  
Rt Pcr ◽  
Protein Expression Level ◽  
Protein Levels ◽  
Serum Biochemical

Objective. To use a rabbit model of parenteral nutrition-associated liver disease (PNALD) to study changes of the endoplasmic reticulum stress (ERS) marker glucose regulatory protein 94 (GRP94) and determine its role in the pathogenesis of PNALD. Methods. A rabbit PNALD model total parenteral nutrition (TPN) group was established. A corresponding control group received breast-feeding for one week. Serum biochemical parameters were measured and liver histological examinations were performed. The level of GRP94 mRNA and protein were measured. Results. The results showed that the serum TBIL, DBIL, and γ-GT levels in the TPN group were significantly higher than those in the control group, while levels of serum ALB in TPN group were significantly lower than those in the control group. The immunohistochemistry results showed that the protein expression level of GRP94 in the liver of TPN group was significantly increased compared with the control group. The RT-PCR results showed that the level of GRP94 mRNA in the liver of the TPN group was significantly higher compared with the control group. Conclusions. The mRNA and protein levels of GRP94 in the TPN group were both significantly increased, indicating that ERS may be directly related to the occurrence and development of PNALD.

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