scholarly journals ANTIFUNGAL SUSCEPTIBILITY OF BIOFILM PRODUCING Candida albicans ISOLATED FROM ORAL CAVITY OF DIABETIC AND NON-DIABETIC POPULATION OF DHARAN, NEPAL

2019 ◽  
Author(s):  
Bijay kumar Shrestha ◽  
Jenish Shakya ◽  
Hemanta Khanal
Keyword(s):  
Drug Resistance ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Antifungal Susceptibility ◽  
Cold Chain ◽  
Diabetic Patients ◽  
Healthy Population ◽  
Oral Rinse ◽  
Diabetic Population ◽  
Oral Candida

Abstract Background: Candida are almost universal on normal adult skin and C. albicans is part of the normal flora of the mucous membranes of the respiratory, gastrointestinal, and female genital tracts. It is acknowledged that diabetic patients are more susceptible to infections caused by Candida albicans due to increased blood glucose and inability of immune system in eradicating the fungus. Studies suggest that Gutkha consumers are also at high risk of oral Candida carriage . Materials and methodology The participants were provided 10 ml of Normal saline and were asked to oral rinse for 1 minute. Oral rinse was collected in a sterile screw capped container and was transported in cold chain to microbiology laboratory. The oral rinse sample was inoculated onto the Sabouraud dextrose agar with Chloramphenicol and was incubated at 37°C for 3-4 days. The Colony forming Unit of candida was compared among diabetic and healthy controls. The candida albicans were identified by Germ tube formation. The Candida albicans isolates were subjected to Biofilm assay, Antifungal susceptibility Test, Haemolysin assay, Haemolysis degree and phospholipase assay. Result: This study reported 31.5% prevalence of oral Candida.The Candida carriage in CFU of diabetic population was statistically significant (p<0.05). The maximum isolates were found to be Biofilm producers. There was significant association between Gutkha consumers with oral Candida carriage. The study suggests that there is higher colonization of Candida in diabetic populations than in healthy population. The result also concludes that frequency of Candida in Oral cavity of Gutkha consumers was also higher (p<0.05). All isolated strains of Candida albicans were tested for antifungal susceptibility testing and 76.19% were found to be Resistant to Fluconazole and 50% were found to be resistant to Amphotericin B. There was statistical significance in Biofilm formation and fluconazole Drug resistance. Conclusion The findings indicated highest colonization of oral Candida in diabetic population and in Gutkha consumers. The greatest numbers of isolated Candida albicans were biofilm producer which showed greater frequency of Fluconazole drug resistance.

Adjuvant Supplementation with L-Carnitine in Diabetic Population and its effects on Lipid Parameters

2021 ◽  
Vol 15 (8) ◽  
pp. 2017-2019
Author(s):  
Rao Salman Aziz ◽  
Usman Saeed ◽  
Nasim Aslam Ghumman ◽  
Muhammad Arshad ◽  
Asif Sohail ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Triglyceride Level ◽  
Clinical Care ◽  
Diabetic Patients ◽  
Healthy Population ◽  
Patients With Diabetes ◽  
Diabetic Population ◽  
Large Discount ◽  
The Impact ◽  
Lipid Parameters

Background: Diabetes is a complicated disease requires continuous clinical care, to govern blood sugar. Aim: To decides the impact of management of L carentin to diabetics at the lipid profile. Methods: This study turned into performed on 120 diabetic Patients had been decided on from endocrinology and diabetes, inside decided on standards. The Patients distributed into three Strata (1st Strata of healthy population and two Strata of patients with diabetes who were on metformin and glibenclamide, one Strata took a L carnitine in a dose of 1000 mg TDS and a Strata dealing with a placebo for a period of ninety days). Results: It is observed those who are on Lcarnitine, confirmed a large discount (p <0.05) with inside the triglyceride level, at the same time as no large adjustments had been located withinside the level of cholesterol and HDL and LDL. Conclusion: These study outcomes that management of L carentin improved profile of lipid in type-2diabetic Patients. Keyword: Dyslipidemia, Diabetes mellitus (DM), l-carnitine (LC).

scholarly journals Role of Matrix β-1,3 Glucan in Antifungal Resistance of Non-albicans Candida Biofilms

2013 ◽  
Vol 57 (4) ◽  
pp. 1918-1920 ◽  
Author(s):  
K. F. Mitchell ◽  
H. T. Taff ◽  
M. A. Cuevas ◽  
E. L. Reinicke ◽  
H. Sanchez ◽  
...  
Keyword(s):  
Health Care ◽  
Drug Resistance ◽  
Candida Albicans ◽  
Care Setting ◽  
Antifungal Susceptibility ◽  
Antifungal Drug ◽  
Content Type ◽  
The Matrix ◽  
Biofilm Matrix

ABSTRACTCandidabiofilm infections pose an increasing threat in the health care setting due to the drug resistance associated with this lifestyle. Several mechanisms underlie the resistance phenomenon. InCandida albicans, one mechanism involves drug impedance by the biofilm matrix linked to β-1,3 glucan. Here, we show this is important for otherCandidaspp. We identified β-1,3 glucan in the matrix, found that the matrix sequesters antifungal drug, and enhanced antifungal susceptibility with matrix β-1,3 glucan hydrolysis.

scholarly journals Genotyping and Antifungal Susceptibility Profile of Sequential Candida albicans Isolated from the Oral Cavity of HIV-Infected Individuals

2018 ◽  
Vol 6 (4) ◽  
Author(s):  
Daniela Vanessa Moris ◽  
Joyce Marinho de Souza ◽  
Márcia Souza Carvalho Melhem ◽  
Marilena dos Anjos Martins ◽  
Caroline Lucio Moreira ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Antifungal Susceptibility

scholarly journals IDENTIFIKASI FAKTOR RISIKO TERJADINYA KOLONISASI SPESIES CANDIDA DI RONGGA MULUT PADA INDIVIDU SEHAT (PENELITIAN PADA MAHASISWA & MAHASISWI FKG UI)

2020 ◽  
Vol 2 (1) ◽  
pp. 023-038
Author(s):  
Audiawati Audiawati ◽  
Siti Aliyah Pradono ◽  
Febrina Rahmayanti
Keyword(s):  
Risk Factors ◽  
Oral Cavity ◽  
Oral Candidiasis ◽  
Salivary Flow ◽  
Oral Health Status ◽  
Blood Type ◽  
Orthodontic Appliances ◽  
Healthy Population ◽  
Chi Square ◽  
Oral Rinse

Introduction : Cases of oral candidiasis are commonly found, both in healthy individuals and immunecompromise patients, however publications of Candida carrier in the oral cavity of healthy population and risk factors for colonization in Indonesia are hardly available. Objective : This study was aimed to analyze the type and number of Candida colonies and identify risk factors in the oral cavity of apparenthly health FKG UI students. Material and methods : the specimens were taken from 195 subjects with oral rinse technique for identification using culture medium CHROMagar® and Sabaraoud dextrose agar. Results and discussion : Candida species were found in the 107 subjects oral cavity (54.87%), being Candida albicans was is the predominant species (52.33%). Some 88 subjects (82.24%) was dominant in the number of colonies 400 CFU/ml, while the rest had colony of 400 CFU/ml (17.76%). Candida colony grew dominantly in single colony (90.65%), and the others showed multi-species colonies (9.34%). Risk factors identified included age; gender; hormonal; blood type O; denture; orthodontic appliances; unstimulated salivary flow; pH of saliva; smoking, alcohol and oral cleaning habit; and oral health status. By using a statistical Pearson chi-square test, no  significant relationship was found between risk factors and number of Candida colonies in the oral cavity p0.05. Conclusion : there was no one single risk factor for Candida colonization, but  combination of various risk factors for demographis, local and systemic was observed

scholarly journals ORAL CANDIDA ALBICANS COLONIZATION RATE IN FIXED ORTHODONTICS PATIENTS

2020 ◽  
Author(s):  
Hussein Mohammad Shoga Al-deen ◽  
Ahmed Ali Obeyah ◽  
Hassan Abdulwahab Al-Shamahy ◽  
Ibrahim Zaid Al-Shami ◽  
Manal Ahmad Saleh AL-amri ◽  
...  
Keyword(s):  
Risk Factors ◽  
Candida Albicans ◽  
Peer Review ◽  
Oral Hygiene ◽  
Candida Species ◽  
Demographic Data ◽  
Oral Rinse ◽  
Orthodontic Patients ◽  
Oral Candida ◽  
E Mail

Objectives:  The objective of this study was to assess the oral Candida albicans colonization ( OCAC)  in a cluster of teenagers and young adults while being treated with a fixed orthodontic appliance (FOA). Subjects and methods:  The investigational group was selected from orthodontic patients whom were examined clinically as soon as to get baseline information before active treatment.  The cluster included 210 patients; 45 males, 165 females (mean age 21.6 ± 4.5 years).  Clinical, demographic data and risk factors were collected in standard questionnaire then each individual was directed to carry out oral wash by a phosphate-buffered saline solution, which was expectorated and processed intended for the isolation of Candida species on Sabouraud’s dextrose agar. The isolated Candida species were identifying by culturing on chromogenic Candida agar and notice species-specific colony natures. Results:  The predominant Candida species isolated was C. albicans with OCAC rate equal to 13.8% extensively enhanced after the insertion of a FOA, as revealed by the oral rinse (P < 0.05) techniques. The results also revealed an increase of OCAC in male patients (24.4%) than female patients (10.9%),   21-25 years patients (17.1%), and regular smoking and Qat chewing were significant associated risk factors (OR=28.6, OR=10.7 respectively , P < 0.0001).  There was no significant association between C. albicans  colonization with oral hygiene in fixed Orthodontic patients. Conclusion:  As a whole, the current data suggest that the introduction of FOA is likely to promote OCAC. Moreover, it becomes visible that the routine oral hygiene procedures performed by these patients may not necessarily reduce OCAC while smoking and chewing Qat habits significantly increased OCAC in FOA. Also smoking and Qat chewing during FOA treatment should be banned if potential harmful effects are to be prevented. Further work with a larger sample size is required to confirm or deny these results. Peer Review History: Received 27 March 2019; Revised 20 April; Accepted 8 May, Available online 15 May 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Name: Dr. A.A. Mgbahurike Affiliation: University of Port Harcourt, Nigeria E-mail: [email protected]   Name: Dr. Tamer Mohamed Ali El Habibi Affiliation: Suez Canal University, Egypt E-mail: [email protected]   Comments of reviewer(s): Similar Articles: ORAL C. ALBICANS COLONIZATION AND NON-CANDIDA ALBICANS CANDIDA COLONIZATION AMONG UNIVERSITY STUDENTS, YEMEN PREVALENCE AND ASSOCIATED FACTORS OF ORAL NON-CANDIDA ALBICANS CANDIDA CARRIAGE IN DENTURE WEARERS IN SANA'A CITY- YEMEN  

scholarly journals ORAL CANDIDA CARRIAGE INCLUDING SPECIATION, BIOFILM PRODUCTION AND CORRELATION WITH OCCURRENCE OF DENTAL CARIES IN STUDENT POPULATION

2019 ◽  
pp. 32-33
Author(s):  
Sumi Nandwani
Keyword(s):  
Candida Albicans ◽  
Dental Caries ◽  
Oral Cavity ◽  
Significant Risk ◽  
Common Species ◽  
Student Population ◽  
Biofilm Production ◽  
Oral Carriage ◽  
Oral Candida

The study was done to establish oral carriage of Candida, speciation, assay biofilm production and correlate the oral Candida carriage with occurrence of dental caries in students. Swab samples from oral cavity were collected from 206 students and were cultured for yeasts. Isolated yeasts were identified and detected for Biofilm production. Results: Overall Candida carriage was found in 90 of the 206 students (75 with caries). The rate of Candida carriage in students with caries (64%) was significantly higher as compared to overall carriage in students (43.7%). A total of 123 Candida strains were isolated including C albicans (63%), C tropicalis (23 %), C. parapsilosis (6 %), C. glabrata (4 %) and C. krusei (4 %). Biofilm production by C albicans was less frequent (42. 85%) than that by non-C albicans (63.33 %). and was significantly higher in students with caries than from without caries. Conclusion: We found a significant risk of dental caries with oral Candida carriage rates in student population. Candida albicans was the most common species isolated. Biofilm production was implicated in Candida virulence and was more important for non- C.albicans than C.albicans strains to establish infections.

scholarly journals Potential Pathogenicity of Candida Species Isolated from Oral Cavity of Patients with Diabetes Mellitus

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Hasti Nouraei ◽  
Mehdi Ghaderian Jahromi ◽  
Leila Razeghian Jahromi ◽  
Kamiar Zomorodian ◽  
Keyvan Pakshir
Keyword(s):  
Diabetes Mellitus ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Hemolytic Activity ◽  
Candida Species ◽  
Extracellular Enzymes ◽  
Oral Candidiasis ◽  
Agar Plate ◽  
Blood Agar Plate ◽  
Diabetic Patients

Introduction. In the recent decade, the increased immunocompromised population such as diabetic patients makes a high incidence of invasive Candida infections. Diabetes mellitus is the most common endocrine metabolic disorder, and diabetic patients are more susceptible to oral candidiasis infection. Candidiasis is an opportunistic fungal infection caused by many species of Candida. Secretion of exoenzymes plays an important role in the virulence and pathogenesis of Candida species. The aim of this study was to evaluate the potential role of phospholipase, esterase, and hemolytic activity of Candida species isolated from oral cavity lesions of diabetic patients. Methods. A total of 108 Candida species including 75 Candida albicans and 33 non-Candida albicans species were recovered from the oral cavity of diabetic patients included in our study. Egg yolk agar, Tween 80 opacity medium, and blood agar plate assays were used for determining phospholipase, esterase, and hemolytic activities, respectively. Results. Candida albicans species had the most exoenzyme activity in comparison to non-albicans isolates. Candida albicans isolates showed 97.3%, 100%, and 77.3% phospholipase, hemolysin, and esterase activities, respectively. The difference between Candida albicans and non-Candida albicans was significant in phospholipase ( P < 0.001 ) and hemolytic activity ( P = 0.027 ), but not significant in esterase activity ( P = 0.076 ). Conclusion. This study showed that most of the isolates had different enzymatic patterns, and Candida albicans isolates had the most exoenzyme activity. So due to the potential effects of these enzymes in pathogenesis and virulence effects of Candida species, we can conclude that the severity of extracellular enzymes may play a role in the severity of signs and symptoms of Candida oral cavity infections in diabetic patients.

scholarly journals Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Kamal Uddin Zaidi ◽  
Abin Mani ◽  
Vijay Thawani ◽  
Arti Mehra
Keyword(s):  
Drug Resistance ◽  
Candida Albicans ◽  
Amphotericin B ◽  
Total Protein ◽  
Opportunistic Infections ◽  
Protein Profile ◽  
Antifungal Susceptibility ◽  
Morphological Difference ◽  
Clinical Isolates ◽  
Clinical Samples

This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

scholarly journals Candida species diversity in oral cavity of type 2 diabetic patients and their In vitro antifungal susceptibility

2018 ◽  
Vol 9 (5) ◽  
pp. 83 ◽  
Author(s):  
Lipsa Bhuyan ◽  
Sahina Hassan ◽  
KailashChandra Dash ◽  
Abikshyeet Panda ◽  
ShyamSundar Behura ◽  
...  
Keyword(s):  
Species Diversity ◽  
Oral Cavity ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
In Vitro Antifungal Susceptibility ◽  
Type 2 Diabetic

scholarly journals In vitro identification and Antifungal susceptibility of different Candida Species isolated from patients with or without Diabetes having Chronic Periodontitis

2015 ◽  
Vol 4 (1) ◽  
pp. 20-25
Author(s):  
Deepti Chaurasia ◽  
Mukesh Gupta ◽  
Reena Kulshrestha ◽  
Jayant Biswas ◽  
Smita D Dutta ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Antifungal Drugs ◽  
Herbal Extract ◽  
Control Group ◽  
Herbal Extracts ◽  
Oral Rinse ◽  
Candidal Species

Background: Candida albicans is the commonly associated species with oral lesions, but other Candida spp., such as C. glabrata, C. tropicalis, C. parapsilosis, C. krusei, and C. dubliniensis have also been isolated from the human saliva, even irrespective to the oral candidiasis. Candidal colonization has also been documented from subgingival plaque of adults with periodontitis.Objective: Nowadays there is a great demand of natural herbal medicines. For a Diabetic person having Periodontitis, the use antifungal drugs are must. Therefore the antifungal capacity of the commonly used drug and herbs has been evaluated.Methods: For the study, the patients were screened and categorised into two study and one control group, having 20 patients in each group, on the basis of their respective Blood Glucose level and Dental status using standard clinical parameters. Oral rinse of 60 patients including both gender were selected from the Out Patient Department of Periodontitis, Rungta College of Dental Sciences and Research, Chhattisgarh. Chrome Candida Agar Media was used to identify Candida albicans and NAC. In vitro tests of the effectiveness of selected antimycotic agents against test candidal isolates recovered from the patient were performed to check the efficacy of the antifungal agents by the Kirby-Bauer Disc Diffusion method.Result: We found that by the use of this chromogenic media most of the commonly recovered NAC can be easily distinguished as each species produced the widest range of colours and morphologies. The herbal extracts Aloe Vera and Garlic failed to inhibit the growth of the candidal species but Neem, Meetha Neem, Triphala, Tulsi showed sensitivity to varying degree. Now a day there are much popularity of using herbal products, having its extracts incorporated in the cosmetics and other products used by humans.Conclusion: We conducted the sensitivity check of the candidal isolates against the dental dentrifices and found that however all the toothpastes contained herbal extracts capable of destroying bacteria but it did not showed good results against candidal species except the Patanjali Toothpaste. This may be due the fact that Patanjali Dant Kanti Dental Cream has the combination of all the herbal extract mixed altogether. DOI: http://dx.doi.org/10.3126/ijosh.v4i1.12164

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