scholarly journals Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

2020 ◽  
Author(s):  
Rafael Schneider ◽  
Muriel Primon-Barros ◽  
Rafael Gomes Von Borowski ◽  
Sophie Chat ◽  
Sylvie Nonin-Lecomte ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Snake Venom ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Cell Damage ◽  
Cell Envelope ◽  
Antibiofilm Activity ◽  
Sequence Alignments

Abstract Background The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections. Results In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis . Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 µM showed that the lowest concentration to inhibit biofilm formation was 25 µM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes. Conclusions Our results suggest that pseudonajide’s positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.

scholarly journals Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

2020 ◽  
Author(s):  
Rafael Schneider ◽  
Muriel Primon-Barros ◽  
Rafael Gomes Von Borowski ◽  
Sophie Chat ◽  
Sylvie Nonin-Lecomte ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Snake Venom ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Cell Damage ◽  
Cell Envelope ◽  
Antibiofilm Activity ◽  
Sequence Alignments

Abstract Background The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals against bacterial infections. Results In this study we present an 11 amino acid-long peptide, named pseudonajide, which is derived from a Pseudonaja textilis venom peptide and has antimicrobial and antibiofilm activity against Staphylococcus epidermidis . Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Antibiofilm activity assays with pseudonajide concentrations ranging from 3.12 to 100 µM showed that the lowest concentration to inhibit biofilm formation was 25 µM. Microscopy analysis demonstrated that pseudonajide interacts with the bacterial cell envelope, disrupting the cell walls and membranes, leading to morphological defects in prokaryotes. Conclusions Our results suggest that pseudonajide’s positives charges interact with negatively charged cell wall components of S. epidermidis, leading to cell damage and inhibiting biofilm formation.

scholarly journals Pseudonajide is an antibiotic peptide derived from snake venom that alter cell wall and membrane integrity interfering on biofilm formation.

2019 ◽  
Author(s):  
Rafael Schneider ◽  
Muriel Primon-Barros ◽  
Rafael Gomes Von Borowski ◽  
Sophie Chat ◽  
Reynald Gillet ◽  
...  
Keyword(s):  
Cell Wall ◽  
Snake Venom ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Cell Damage ◽  
Cell Envelope ◽  
Membrane Integrity ◽  
Antibiofilm Activity ◽  
Sequence Alignments ◽  
Resistance Phenotype

Abstract Background The increase of bacterial resistance phenotype cases is a global health problem. New strategies in scientific community must be explored in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. These molecules have highly diverse targets in prokaryotic cells, making resistance phenotype development more difficult. Results In this study we present a peptide of just 11 amino acids which has antimicrobial and antibiofilm activity against Staphyloccocus epidermidis. Named pseudonajide, it is derived from Pseudonaja textilis venom. Pseudonajide was selected based on the sequence alignments of various snake venom peptides that displayed activity against bacteria. Several concentrations of pseudonajide were tested in antibiofilm activity essay, it was detected that 25 µM was the best minimal concentration for biofilm inhibiting activity. Microscopy analysis demonstrates that pseudonajide interacts with the bacterial cell envelope, disrupting the cell wall and membrane leading to morphological defects in prokaryotes. Conclusions Our results suggest that pseudonajide’s positives charges interacts with negative charged cell wall components of S. epidermidis. Leading to cell damage and biofilm formation inhibition.

scholarly journals Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

2020 ◽  
Author(s):  
Rafael Schneider ◽  
Muriel Primon-Barros ◽  
Rafael Gomes Von Borowski ◽  
Sophie Chat ◽  
Reynald Gillet ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Staphylococcus Epidermidis ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Cell Envelope ◽  
New Treatment ◽  
Treatment Alternatives ◽  
Animal Venoms ◽  
Global Health Problem ◽  
New Strategies

Abstract The increase of bacterial resistance phenotype cases is a global health problem. New strategies in scientific community must be explored in order to create new treatment alternatives. Animal venoms are a good source for antimicrobial peptides (AMPs), which are excellent candidates for new antimicrobial drug development. Cathelicidin-related antimicrobial peptides (CRAMPs) from snake venoms have been studied as a model for the design of new antimicrobial pharmaceuticals at bacterial infections setting.

scholarly journals Pseudonajide peptide derived from snake venom alters cell envelope integrity interfering on biofilm formation in Staphylococcus epidermidis

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Rafael Schneider ◽  
Muriel Primon-Barros ◽  
Rafael Gomes Von Borowski ◽  
Sophie Chat ◽  
Sylvie Nonin-Lecomte ◽  
...  
Keyword(s):  
Snake Venom ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Cell Envelope

scholarly journals Antimicrobial and Antibiofilm Peptides

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 652 ◽  
Author(s):  
Angela Di Somma ◽  
Antonio Moretta ◽  
Carolina Canè ◽  
Arianna Cirillo ◽  
Angela Duilio
Keyword(s):  
Antimicrobial Peptides ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Antibiofilm Activity ◽  
Chronic Infections ◽  
Planktonic Bacteria ◽  
Hostile Environments

The increasing onset of multidrug-resistant bacteria has propelled microbiology research towards antimicrobial peptides as new possible antibiotics from natural sources. Antimicrobial peptides are short peptides endowed with a broad range of activity against both Gram-positive and Gram-negative bacteria and are less prone to trigger resistance. Besides their activity against planktonic bacteria, many antimicrobial peptides also show antibiofilm activity. Biofilms are ubiquitous in nature, having the ability to adhere to virtually any surface, either biotic or abiotic, including medical devices, causing chronic infections that are difficult to eradicate. The biofilm matrix protects bacteria from hostile environments, thus contributing to the bacterial resistance to antimicrobial agents. Biofilms are very difficult to treat, with options restricted to the use of large doses of antibiotics or the removal of the infected device. Antimicrobial peptides could represent good candidates to develop new antibiofilm drugs as they can act at different stages of biofilm formation, on disparate molecular targets and with various mechanisms of action. These include inhibition of biofilm formation and adhesion, downregulation of quorum sensing factors, and disruption of the pre-formed biofilm. This review focuses on the proprieties of antimicrobial and antibiofilm peptides, with a particular emphasis on their mechanism of action, reporting several examples of peptides that over time have been shown to have activity against biofilm.

scholarly journals N-alkylimidazolium Salts Functionalized with p-Coumaric and Cinnamic Acid: A Study of Their Antimicrobial and Antibiofilm Effects

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3484
Author(s):  
Oscar Forero-Doria ◽  
Ramiro Araya-Maturana ◽  
Anggela Barrientos-Retamal ◽  
Luis Morales-Quintana ◽  
Luis Guzmán
Keyword(s):  
Antibacterial Activity ◽  
Cinnamic Acid ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Good Alternative ◽  
Antibiofilm Activity ◽  
Coumaric Acid ◽  
Cinnamic Acids ◽  
Gram Positive Bacteria ◽  
Therapeutic Alternatives

The bacterial resistance to antibiotics has compromised the therapies used for bacterial infections. Nowadays, many strategies are being carried out to address this problem. Among them, the use of natural compounds like cinnamic and p-coumaric acids stands out. Nevertheless, their utilization is limited because of their unfavorable physicochemical properties. Due to the lack of new therapeutic alternatives for bacterial infections, novel strategies have emerged, such as the use of ionic liquids; given that they can show a broad spectrum of antibacterial activity, this is why we herein report the antibacterial and antibiofilm activity of a series of N-alkylimidazolium salts functionalized with p-coumaric and cinnamic acids. The results from this study showed better antibacterial activity against Gram-positive bacteria, with a predominance of the salts derived from coumaric acid and a correlation with the chain length. Additionally, a lower efficacy was observed in the inhibition of biofilm formation, highlighting the antibiofilm activity against Staphylococcus aureus, which decreased the production of the biofilm by 52% over the control. In conclusion, we suggest that the salts derived from p-coumaric acid are good alternatives as antibacterial compounds. Meanwhile, the salt derived from cinnamic acid could be a good alternative as an antibiofilm compound.

scholarly journals Aryl Rhodanines Specifically Inhibit Staphylococcal and Enterococcal Biofilm Formation

2009 ◽  
Vol 53 (10) ◽  
pp. 4357-4367 ◽  
Author(s):  
Timothy J. Opperman ◽  
Steven M. Kwasny ◽  
John D. Williams ◽  
Atiyya R. Khan ◽  
Norton P. Peet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Small Molecules ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Artificial Surfaces ◽  
Causative Agents ◽  
Biofilm Development

ABSTRACT Staphylococcus epidermidis and Staphylococcus aureus are the leading causative agents of indwelling medical device infections because of their ability to form biofilms on artificial surfaces. Here we describe the antibiofilm activity of a class of small molecules, the aryl rhodanines, which specifically inhibit biofilm formation of S. aureus, S. epidermidis, Enterococcus faecalis, E. faecium, and E. gallinarum but not the gram-negative species Pseudomonas aeruginosa or Escherichia coli. The aryl rhodanines do not exhibit antibacterial activity against any of the bacterial strains tested and are not cytotoxic against HeLa cells. Preliminary mechanism-of-action studies revealed that the aryl rhodanines specifically inhibit the early stages of biofilm development by preventing attachment of the bacteria to surfaces.

Combating bacterial resistance by combination of antibiotics with antimicrobial peptides

2019 ◽  
Vol 91 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Dean E. Sheard ◽  
Neil M. O’Brien-Simpson ◽  
John D. Wade ◽  
Frances Separovic
Keyword(s):  
Antimicrobial Peptides ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Synergistic Effects ◽  
Resistance Mechanisms ◽  
Bacterial Strains ◽  
Diverse Range ◽  
Recent Emergence ◽  
Agricultural Industries ◽  
Conventional Antibiotics

Abstract The overuse of antibiotics in the healthcare and agricultural industries has led to the worldwide spread of bacterial resistance. The recent emergence of multidrug resistant (MDR) bacteria has resulted in a call for the development of novel strategies to address this global issue. Research on a diverse range of antimicrobial peptides (AMPs) has shown promising activity against several resistant strains. Increased understanding of the mode of action of AMPs has shown similarity and complementarity to conventional antibiotics and the combination of both has led to synergistic effects in some cases. Combination therapy has been widely used to combat MDR bacterial infections and the recent focus on their application with AMPs may allow antibiotics to be effective against resistant bacterial strains. By conjugation of an antibiotic onto an AMP, a compound may be produced with possibly greater activity and with reduced side-effects and toxicity. The AMP in these conjugates may also act as a unique adjuvant for the antibiotic by disrupting the resistance mechanisms used by bacteria thus allowing the antibiotic to once again be effective. This mini-review outlines some of the current and past work in combining AMPs with conventional antibiotics as strategies to address bacterial resistance.

scholarly journals Synergistic antibacterial activity of monoterpenes in combination with conventional antimicrobials against Gram-positive and Gram-negative bacteria

2020 ◽  
Vol 19 (2) ◽  
pp. 258
Author(s):  
Maria Helena Pereira de Lira ◽  
Gustavo Fernandes Queiroga Moraes ◽  
Girlene Macena Santos ◽  
Francisco Patrício de Andrade Júnior ◽  
Fillipe De Oliveira Pereira ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Inhibitory Concentration ◽  
Synergistic Effects ◽  
Public Health Problem ◽  
New Drugs ◽  
Gram Negative Bacteria ◽  
Microdilution Method

<p><strong>Introduction</strong>: bacterial infections are a public health problem. Besides, the emergence of strains resistant to antimicrobials has<br />contributed to the search for new alternatives, such for the terpenes with antimicrobial potential. <strong>Objectives</strong>: the objective of this<br />study was to determine the possible interaction of isolated monoterpenes (-)-Carveol, Geraniol, Citronellol, α-terpineol, R-(-) Carvone,<br />(-)-Menthol, Linalool, D-Dihydrocarvone, and (-)-Terpine-4-ol with conventional antimicrobials (Chloramphenicol, Minocycline,<br />Amoxicillin and Ciprofloxacin) when they are evaluated on Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis,<br />Escherichia coli and Pseudomonas aeruginosa strains. <strong>Methodology</strong>: the minimum inhibitory concentrations of these test drugs<br />were determined using the microdilution method. The Checkerboard method was used to assess the interactions, by determining<br />the fractional inhibitory concentration index (FIC index). <strong>Results</strong>: aamong the monoterpenes, only Carveol, Citronellol, and Geraniol<br />presented antimicrobial activity (MIC &lt; 1024 μg/mL). They presented synergistic effects against Pseudomonas aeruginosa ATCC-9027<br />(FIC index ≤ 0.5) when in combination with Minocycline. <strong>Conclusion</strong>: this study contributes to the development of new approaches<br />to control bacterial resistance and to the possibility of discovering new drugs.</p>

scholarly journals Imidazole-Thiosemicarbazide Derivatives as Potent Anti-Mycobacterium tuberculosis Compounds with Antibiofilm Activity

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3476
Author(s):  
Adrian Bekier ◽  
Malwina Kawka ◽  
Jakub Lach ◽  
Jarosław Dziadek ◽  
Agata Paneth ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Effective Vaccine ◽  
Antibiofilm Activity ◽  
Intracellular Growth ◽  
Expensive Drug ◽  
Cytotoxicity Studies ◽  
Thiosemicarbazide Derivatives

Mycobacterium tuberculosis (Mtb) is an intracellular pathogenic bacterium and the causative agent of tuberculosis. This disease is one of the most ancient and deadliest bacterial infections, as it poses major health, social and economic challenges at a global level, primarily in low- and middle-income countries. The lack of an effective vaccine, the long and expensive drug therapy, and the rapid spread of drug-resistant strains of Mtb have led to the re-emergence of tuberculosis as a global pandemic. Here, we assessed the in vitro activity of new imidazole-thiosemicarbazide derivatives (ITDs) against Mtb infection and their effects on mycobacterial biofilm formation. Cytotoxicity studies of the new compounds in cell lines and human monocyte-derived macrophages (MDMs) were performed. The anti-Mtb activity of ITDs was evaluated by determining minimal inhibitory concentrations of resazurin, time-kill curves, bacterial intracellular growth and the effect on biofilm formation. Mutation frequency and whole-genome sequencing of mutants that were resistant to ITDs were performed. The antimycobacterial potential of ITDs with the ability to penetrate Mtb-infected human macrophages and significantly inhibit the intracellular growth of tubercle bacilli and suppress Mtb biofilm formation was observed.

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