Draft genome sequence of multidrug-resistant Bradyrhizobium sp. BL isolated from a sewage treatment plant in China

2019 ◽  
Author(s):  
Peng Bao ◽  
Guo-Xiang Li ◽  
Yu-Qin He ◽  
Yi Dai
Keyword(s):  
Antibiotic Resistance ◽  
Municipal Wastewater ◽  
Draft Genome ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Genomic Diversity ◽  
Comparative Genomic ◽  
Municipal Wastewater Treatment ◽  
Total Size ◽  
Bradyrhizobium Sp

Abstract The genus Bradyrhizobium is considered to be widespread and abundant group of symbiotic bacteria in many plant-soil ecosystems. However, the ecological versatility of this phylogenetic group remains highly understudied in man-made ecosystems, mainly due to the lack of pure cultures and genomic data. To further expand our understanding of this genus for human health, we analyzed the high quality draft genome of Bradyrhizobium strain BL, isolated from a municipal wastewater treatment plant in Ningbo, China. The Bradyrhizobium sp. BL draft genome has a total size of 7,718,431 bp with an overall G + C content of 46.43%. From a total of 7236 predicted sequences, 7176 and 60 are protein and RNA coding sequences, respectively. Moreover, 63.51% of the predicted genes were assigned into to Clusters of Orthologous Groups (COG) functional categories. The Bradyrhizobium sp. BL genome contains various defense mechanisms against antibiotics that up to predicted 60 antibiotic resistance coding genes. The Bradyrhizobium sp. BL genome contains 237 termed virulence factors coding genes which show its potential pathogenicity. This study provides important insights into the genomic diversity of the genus Bradyrhizobium and provides a foundation for future comparative genomic studies that will generate a better understanding of the antibiotic resistance process.

Treatment of Rural Domestic Sewage by Solar Ozonic Ecological Sewage Treatment Plant

2014 ◽  
Vol 955-959 ◽  
pp. 3393-3399 ◽  
Author(s):  
Wei Zheng ◽  
Yan Ming Yang ◽  
Yun Long Li ◽  
Jian Qiu Zheng
Keyword(s):  
Municipal Wastewater ◽  
Operating Cost ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Domestic Sewage ◽  
Design Parameters ◽  
Small Scale ◽  
Municipal Wastewater Treatment ◽  
Average Percentage

The process technique and design parameters of project of Solar Ozonic Ecological Sewage Treatment Plant (short for SOESTP) which consists of anaerobic reactor, horizontal subsurface flow (HSSF) constructed wetlands(CWs) and the combination of solar power and ozone disinfection are described, the paper further examines the removal efficiency for treating rural domestic sewage, running expense and recycling ability of product water. The results show that the average percentage removal values of CODcr，BOD5，SS，TN，NH3-N，TP range from 95.6% to 98.0%, 96.0% to 98.7%, 93.1% to 96.1%, 97.0% to 98.9%, 96.9% to 99.5%, 98.2% to 99.6%, respectively, the reduction of fecal coliform (FC) reaches 99.9%, the effluent quality meets the first level A criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant（GB18918-2002）. The running cost of SOESTP is 0.063yuan/ m3, saves much more than traditional sewage treatment, and the ozone water obtained from the reservoir will be an ideal choice for disinfection .The system has characteristics of easy manipulation, low operating cost, achieving advanced water, energy conservation and environment protection, is thought to be very suitable for use as the promotion of rural small - scale sewage treatment.

scholarly journals Prevalence of Antibiotic-Resistant Bacteria ESKAPE among Healthy People Estimated by Monitoring of Municipal Wastewater

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 495
Author(s):  
Masateru Nishiyama ◽  
Susan Praise ◽  
Keiichi Tsurumaki ◽  
Hiroaki Baba ◽  
Hajime Kanamori ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
General Population ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Municipal Wastewater Treatment ◽  
Antibiotic Resistant ◽  
Carbapenem Resistant ◽  
Wastewater Samples

There is increasing attention toward factors that potentially contribute to antibiotic resistance (AR), as well as an interest in exploring the emergence and occurrence of antibiotic resistance bacteria (ARB). We monitored six ARBs that cause hospital outbreaks in wastewater influent to highlight the presence of these ARBs in the general population. We analyzed wastewater samples from a municipal wastewater treatment plant (MWWTP) and hospital wastewater (HW) for six species of ARB: Carbapenem-resistant Enterobacteria (CARBA), extended-spectrum β-lactamase producing Enterobacteria (ESBL), multidrug-resistant Acinetobacter (MDRA), multidrug-resistant Pseudomonas aeruginosa (MDRP), methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant Enterococci (VRE). We registered a high percentage of ARBs in MWWTP samples (>66%) for all ARBs except for MDRP, indicating a high prevalence in the population. Percentages in HW samples were low (<78%), and no VRE was detected throughout the study. CARBA and ESBL were detected in all wastewater samples, whereas MDRA and MRSA had a high abundance. This result demonstrated the functionality of using raw wastewater at MWWTP to monitor the presence and extent of ARB in healthy populations. This kind of surveillance will contribute to strengthening the efforts toward reducing ARBs through the detection of ARBs to which the general population is exposed.

scholarly journals Erythromycin Resistance-Conferring Plasmid pRSB105, Isolated from a Sewage Treatment Plant, Harbors a New Macrolide Resistance Determinant, an Integron-Containing Tn402-Like Element, and a Large Region of Unknown Function

2007 ◽  
Vol 73 (6) ◽  
pp. 1952-1960 ◽  
Author(s):  
A. Schlüter ◽  
R. Szczepanowski ◽  
N. Kurz ◽  
S. Schneiker ◽  
I. Krahn ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Unknown Function ◽  
Municipal Wastewater ◽  
Ralstonia Eutropha ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Macrolide Resistance ◽  
Municipal Wastewater Treatment ◽  
Erythromycin Resistance ◽  
Modification System

ABSTRACT The erythromycin resistance plasmid pRSB105 was previously isolated from an activated sludge bacterial community of a municipal wastewater treatment plant. Compilation of the complete pRSB105 nucleotide sequence revealed that the plasmid is 57,137 bp in size and has a mean G+C content of 56.66 mol%. The pRSB105 backbone is composed of two different replication and/or partitioning modules and a functional mobilization region encoding the mobilization genes mobCDE and mobBA. The first replicon (Rep1) is nearly identical to the corresponding replication module of the multiresistance plasmid pRSB101 isolated from an unknown activated sludge bacterium. Accordingly, pRSB101 and pRSB105 are sister plasmids belonging to a new plasmid family. The second replicon (Rep2) of pRSB105 was classified as a member of the IncP-6 group. While Rep1 confers replication ability only in γ-proteobacteria, Rep2 extents the host range of the plasmid since it is also functional in the β-proteobacterium Ralstonia eutropha. Plasmid pRSB105 harbors the macrolide resistance genes mel and mph, encoding, respectively, a predicted ABC-type efflux permease and a macrolide-2′-phosphotransferase. Erythromycin resistance is mainly attributed to mel, whereas mph contributes to erythromycin resistance to a lesser extent. The second resistance region, represented by an integron-containing Tn402-like element, includes a β-lactam (oxa10) and a trimethoprim (dfrB2) resistance gene cassette. In addition to antibiotic resistance modules, pRSB105 encodes a functional restriction/modification system and two nonresistance regions of unknown function. The presence of different mobile genetic elements that flank resistance and nonresistance modules on pRSB105 indicates that these elements were involved in acquisition of accessory plasmid modules. Comparative genomics of pRSB105 and related plasmids elucidated that pRSB105 evolved by integration of distinct modules from different plasmid sources, including Pseudomonas aeruginosa plasmids, and thus represents a mosaic plasmid.

scholarly journals Identification of Biogenic Sulphate Corrosion of Concrete in Sewage Treatment Plant Settling Tank Walls

2020 ◽  
Vol 30 (3) ◽  
pp. 253-264
Author(s):  
Łukasz Krysiak ◽  
Paweł Falaciński ◽  
Łukasz Szarek
Keyword(s):  
Sulphuric Acid ◽  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Settling Tank ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Cement Matrix ◽  
Wastewater Management ◽  
Municipal Wastewater Treatment ◽  
Biochemical Processes

Abstract One of the basic threats in terms of concrete used for tanks or ducts applied in wastewater management is the phenomenon of biogenic sulphate corrosion (BSC). BSC is a particular case of corrosion caused by the action of sulphuric acid (IV), which is formed as a result of a number of biochemical processes, which can take place, e.g. in an environment encountered within the aforementioned structures. Ions present in sulphuric acid react with cement hydration products, which leads to replacing the primary cement matrix components with compounds easily-soluble or highly-swelling during crystallization. The outcome of advanced corrosion is usually an observed formation of a white, amorphous, sponge-like mass, which is easily separated from the underlying concrete. The article discusses a case study of a BSC process in a newly constructed primary settling tank in a municipal wastewater treatment plant.

Engineering Research on ECOSUNIDE Technique for Nutrient Removal from Municipal Sewage

2011 ◽  
Vol 383-390 ◽  
pp. 3422-3427
Author(s):  
Yan Li ◽  
Yan Qiu Zhang
Keyword(s):  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Experimental System ◽  
Class I ◽  
Municipal Sewage ◽  
Low Carbon ◽  
Municipal Wastewater Treatment ◽  
Engineering Research

For engineering research, ECOSUNIDE technique was applied in a sewage treatment plant under low temperature and low carbon resource. The whole experimental system has being running steadily after reasonable adjustments of influent distribution, returned sludge ratio and DO. The effluent qualities meet the Class I-B Criteria Specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB18918-2002).Effluent BOD5 and NH3-N meet the Class I-A Criteria. The results indicates that the treatment capacity could fluctuate within a range of ±3000 m3/d with DO properly controlled. In aerobic sections, the best DO was 2-3 mg/L. The optimal sludge emission amount could be attained through settlement ratio and sludge concentration with the present emission amount of sludge of 680 m3/d. It might be the restrictive factor that BOD/TN was less than 3 on TN removal which could not steadily achieve the Class I-A Criteria.

scholarly journals Draft Genome Sequence of the Interspecies Hybrid Saccharomyces pastorianus Strain HA2560, Isolated from a Municipal Wastewater Treatment Plant

2018 ◽  
Vol 6 (17) ◽  
Author(s):  
Hakim Tafer ◽  
Katja Sterflinger ◽  
Ksenija Lopandic
Keyword(s):  
Wastewater Treatment ◽  
Genome Sequence ◽  
Wastewater Treatment Plant ◽  
Municipal Wastewater ◽  
Draft Genome ◽  
Treatment Plant ◽  
Draft Genome Sequence ◽  
Municipal Wastewater Treatment ◽  
Content Type ◽  
Saccharomyces Pastorianus

ABSTRACT Saccharomyces pastorianus is an industrially relevant yeast frequently isolated from brewing environments. Here, we report the draft genome sequence of the S. pastorianus HA2560 strain isolated from a municipal wastewater treatment plant.

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