scholarly journals Gut Microbiota Related to Fiber Digestibility were Identified by Variation of Apparent Fiber Digestibility in Chinese Suhuai pig

2020 ◽  
Author(s):  
Qing Niu ◽  
Guang Pu ◽  
Lijuan Fan ◽  
Chen Gao ◽  
Huan Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Neutral Detergent Fiber ◽  
Rrna Gene ◽  
Finishing Pigs ◽  
Gut Health ◽  
Mean Values ◽  
Fiber Digestibility ◽  
Rrna Gene Sequencing ◽  
Potential Biomarkers

Abstract Background: Dietary fiber plays an important role in porcine gut health and welfare. Fiber mainly degraded by the gut microbiota, whereas most gut microbiota related to fiber digestibility of pigs are still unidentified. To reveal gut microbiota associated with apparent digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF), apparent NDF, ADF digestibility of 274 Suhuai female finishing pigs at the age of 160 days were measured. The gut microbiota of Suhuai pigs were analyzed through 16S rRNA gene sequencing, respectively. Results: Large phenotypic variations in apparent NDF and ADF digestibility were separately found among Suhuai pigs. The coefficient of variation of NDF and ADF digestibility was 12.08% and 18.08%, respectively. The mean values of digestibility of H-NDF and H-ADF groups were 30.20% and 33.76% more than those of the L-NDF and L-ADF groups (P＜0.01), respectively. A total of 927 and 935 operational taxonomic units (OTUs) were confirmed from two types of fecal samples,respectively. There were14 phyla in all samples and the abundances of Spirochaetae, Bacteroidetes and unclassified_k__norank were significantly different between H-NDF and L-NDF groups (P＜0.05) and the abundances of Spirochaetae, Verrucomicrobia, unclassified_k__norank and Fibrobactere were significantly different between H-ADF and L-ADF group (P＜0.05).A total of 188, 183, 188 and 185 genera were separately identified in H-NDF, L-NDF, H-ADF and L-ADF groups, while 6, 1, 5 and 2 genera were separately specific to H-NDF, L-NDF, H-ADF and L-ADF groups. The microbiota of H-NDF and H-ADF clustered separately from the microbiota of the L-NDF and L-ADF along principal coordinate 1, respectively. Compared with L-NDF group, 29 genera were found to be potential biomarkers in H-NDF group. Compared with L-ADF group, 23 genera were found to be potential biomarkers in H-ADF group. The most important functions and metabolic pathways of the above potential biomarkers included carbohydrate transport and metabolism. Conclusions: Microbial community structures were significantly different between high and low fiber digestibility groups. Twenty nine and 23 genera were found to be potential biomarkers in H-NDF and H-ADF group, respectively. The biomarkers may be the key functional microbiota related to apparent fiber digestibility.

scholarly journals A mixture of quebracho and chestnut tannins drives butyrate-producing bacteria populations shift in the gut microbiota of weaned piglets

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250874
Author(s):  
Francesco Miragoli ◽  
Vania Patrone ◽  
Aldo Prandini ◽  
Samantha Sigolo ◽  
Matteo Dell’Anno ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Dietary Intervention ◽  
16S Rrna Gene Sequencing ◽  
Illumina Miseq ◽  
Control Group ◽  
Central Position ◽  
Rrna Gene ◽  
Gut Health ◽  
Inorganic Ion ◽  
Rrna Gene Sequencing

Weaning is a critical period for piglets, in which unbalanced gut microbiota and/or pathogen colonisation can contribute to diseases that interfere with animal performance. Tannins are natural compounds that could be used as functional ingredients to improve gut health in pig farming thanks to their antibacterial, antioxidant, and antidiarrhoeal properties. In this study, a mixture of quebracho and chestnut tannins (1.25%) was evaluated for its efficacy in reducing the negative weaning effects on piglet growth. Microbiota composition was assessed by Illumina MiSeq 16S rRNA gene sequencing of DNA extracted from stools at the end of the trial. Sequence analysis revealed an increase in the genera Shuttleworthia, Pseudobutyrivibrio, Peptococcus, Anaerostipes, and Solobacterium in the tannin-supplemented group. Conversely, this dietary intervention reduced the abundance of the genera Syntrophococcus, Atopobium, Mitsuokella, Sharpea, and Prevotella. The populations of butyrate-producing bacteria were modulated by tannin, and higher butyrate concentrations in stools were detected in the tannin-fed pigs. Co-occurrence analysis revealed that the operational taxonomic units (OTUs) belonging to the families Veillonellaceae, Lachnospiraceae, and Coriobacteriaceae occupied the central part of the network in both the control and the tannin-fed animals. Instead, in the tannin group, the OTUs belonging to the families Acidaminococcaceae, Alcaligenaceae, and Spirochaetaceae characterised its network, whereas Family XIII Incertae Sedis occupied a more central position than in the control group. Conversely, the presence of Desulfovibrionaceae characterised the network of the control group, and this family was not present in the network of the tannin group. Moreover, the prediction of metabolic pathways revealed that the gut microbiome of the tannin group possessed an enhanced potential for carbohydrate transport and metabolism, as well as a lower abundance of pathways related to cell wall/membrane/envelope biogenesis and inorganic ion transport. In conclusion, the tested tannins seem to modulate the gut microbiota, favouring groups of butyrate-producing bacteria.

Dynamic establishment of the gut bacterial ecology of cows from birth to adulthood

2019 ◽  
Author(s):  
Chunyan Guo ◽  
Shoukun Ji ◽  
Hui Yan ◽  
Yajing Wang ◽  
Jingjing Liu ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Microbiota ◽  
Major Change ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Gut Health ◽  
Gastrointestinal Microbiota ◽  
Bacterial Ecology ◽  
Ecological Applications ◽  
Rrna Gene Sequencing

Abstract Background: The gut microbiota plays multiple critical roles in maintaining the health of the host, especially in ruminants; however, our understanding of the process of gut microbiota establishment from birth to adulthood remains limited. Here, we investigated the bacterial ecology in the gastrointestinal segments (rumen, abomasum, duodenum and rectum) of Holstein cows from 1 week to 5 years of age by 16S rRNA gene sequencing to illustrate the dynamic establishment of gastrointestinal microbiota of cows. Results: We revealed a major change in the composition, diversity, and abundance of bacteria with age increase. Remarkably, the gut bacterial community was found to be age-discriminatory, and microbiota gradually matured in each segment of the gut which followed a natural logarithmic function by applying a maturity index algorithm, that meant gut microbiota was established quickly in young animals, then slowed with age. Using the Bayesian algorithm of Source-Tracker, we demonstrated that the proportion of bacterial transfer from the foregut to the hindgut was also higher in young animals, and then decreased sharply with age. Conclusions: Our findings demonstrated gastrointestinal microbiota was established quickly during young period of cows and highlighted the “window period” of 18 weeks after birth for cows to establish their gut bacterial community. This insight into gut microbiota might have important ecological applications in protecting gut health or manipulating the gut microbiota of cows.

scholarly journals Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 254
Author(s):  
Ying Wang ◽  
Jianqing Zhu ◽  
Jie Fang ◽  
Li Shen ◽  
Shuojia Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
16S Rrna Gene Sequencing ◽  
Adult Survival ◽  
Rrna Gene ◽  
Life Stages ◽  
Microbial Composition ◽  
Significant Difference ◽  
Functional Inference ◽  
Rrna Gene Sequencing ◽  
Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

scholarly journals Gut microbiota markers associated with obesity and overweight in Italian adults

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vanessa Palmas ◽  
Silvia Pisanu ◽  
Veronica Madau ◽  
Emanuela Casula ◽  
Andrea Deledda ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Relative Abundance ◽  
Smoking Status ◽  
16S Rrna Gene Sequencing ◽  
Normal Weight ◽  
Activity Level ◽  
Rrna Gene ◽  
Illumina Platform ◽  
Obesity And Overweight ◽  
Rrna Gene Sequencing

AbstractIn the present study, we characterized the distinctive signatures of the gut microbiota (GM) from overweight/obese patients (OB), and normal-weight controls (NW), both of Sardinian origin. Fecal bacterial composition of 46 OB patients (BMI = 36.6 ± 6.0; F/M = 40/6) was analyzed and compared to that of 46 NW subjects (BMI = 21.6 ± 2.1; F/M = 41/5), matched for sex, age and smoking status, by using 16S rRNA gene sequencing on MiSeq Illumina platform. The gut microbial community of OB patients exhibited a significant decrease in the relative abundance of several Bacteroidetes taxa (i.e. Flavobacteriaceae, Porphyromonadaceae, Sphingobacteriaceae, Flavobacterium, Rikenella spp., Pedobacter spp., Parabacteroides spp., Bacteroides spp.) when compared to NW; instead, several Firmicutes taxa were significantly increased in the same subjects (Lachnospiraceae, Gemellaceae, Paenibacillaceae, Streptococcaceae, Thermicanaceae, Gemella, Mitsuokella, Streptococcus, Acidaminococcus spp., Eubacterium spp., Ruminococcus spp., Megamonas spp., Streptococcus, Thermicanus, Megasphaera spp. and Veillonella spp.). Correlation analysis indicated that body fatness and waist circumference negatively correlated with Bacteroidetes taxa, while Firmicutes taxa positively correlated with body fat and negatively with muscle mass and/or physical activity level. Furthermore, the relative abundance of several bacterial taxa belonging to Enterobacteriaceae family, known to exhibit endotoxic activity, was increased in the OB group compared to NW. The results extend our knowledge on the GM profiles in Italian OB, identifying novel taxa linking obesity and intestine.

scholarly journals Gut microbiota accelerates cisplatin-induced acute liver injury associated with robust inflammation and oxidative stress in mice

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Shenhai Gong ◽  
Yinglin Feng ◽  
Yunong Zeng ◽  
Huanrui Zhang ◽  
Meiping Pan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
16S Rrna ◽  
Gut Microbiota ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Metabolomic Analysis ◽  
Rrna Gene Sequencing ◽  
And Oxidative Stress

Abstract Background Gut microbiota has been reported to be disrupted by cisplatin, as well as to modulate chemotherapy toxicity. However, the precise role of intestinal microbiota in the pathogenesis of cisplatin hepatotoxicity remains unknown. Methods We compared the composition and function of gut microbiota between mice treated with and without cisplatin using 16S rRNA gene sequencing and via metabolomic analysis. For understanding the causative relationship between gut dysbiosis and cisplatin hepatotoxicity, antibiotics were administered to deplete gut microbiota and faecal microbiota transplantation (FMT) was performed before cisplatin treatment. Results 16S rRNA gene sequencing and metabolomic analysis showed that cisplatin administration caused gut microbiota dysbiosis in mice. Gut microbiota ablation by antibiotic exposure protected against the hepatotoxicity induced by cisplatin. Interestingly, mice treated with antibiotics dampened the mitogen-activated protein kinase pathway activation and promoted nuclear factor erythroid 2-related factor 2 nuclear translocation, resulting in decreased levels of both inflammation and oxidative stress in the liver. FMT also confirmed the role of microbiota in individual susceptibility to cisplatin-induced hepatotoxicity. Conclusions This study elucidated the mechanism by which gut microbiota mediates cisplatin hepatotoxicity through enhanced inflammatory response and oxidative stress. This knowledge may help develop novel therapeutic approaches that involve targeting the composition and metabolites of microbiota.

scholarly journals Comparison between 16S rRNA and shotgun sequencing data for the taxonomic characterization of the gut microbiota

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Francesco Durazzi ◽  
Claudia Sala ◽  
Gastone Castellani ◽  
Gerardo Manfreda ◽  
Daniel Remondini ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Shotgun Sequencing ◽  
Rrna Gene ◽  
Metagenomic Sequencing ◽  
Experimental Conditions ◽  
Rrna Gene Sequencing

AbstractIn this paper we compared taxonomic results obtained by metataxonomics (16S rRNA gene sequencing) and metagenomics (whole shotgun metagenomic sequencing) to investigate their reliability for bacteria profiling, studying the chicken gut as a model system. The experimental conditions included two compartments of gastrointestinal tracts and two sampling times. We compared the relative abundance distributions obtained with the two sequencing strategies and then tested their capability to distinguish the experimental conditions. The results showed that 16S rRNA gene sequencing detects only part of the gut microbiota community revealed by shotgun sequencing. Specifically, when a sufficient number of reads is available, Shotgun sequencing has more power to identify less abundant taxa than 16S sequencing. Finally, we showed that the less abundant genera detected only by shotgun sequencing are biologically meaningful, being able to discriminate between the experimental conditions as much as the more abundant genera detected by both sequencing strategies.

scholarly journals Infant Gut Microbiota Associated with Fine Motor Skills

Nutrients ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 1673
Author(s):  
Inmaculada Acuña ◽  
Tomás Cerdó ◽  
Alicia Ruiz ◽  
Francisco J. Torres-Espínola ◽  
Ana López-Moreno ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Infant Development ◽  
16S Rrna Gene Sequencing ◽  
Fine Motor ◽  
Rrna Gene ◽  
Neurodevelopmental Outcomes ◽  
Gut Colonization ◽  
Healthy Infants ◽  
Behavioural Characteristics ◽  
Rrna Gene Sequencing

BACKGROUND: During early life, dynamic gut colonization and brain development co-occur with potential cross-talk mechanisms affecting behaviour. METHODS: We used 16S rRNA gene sequencing to examine the associations between gut microbiota and neurodevelopmental outcomes assessed by the Bayley Scales of Infant Development III in 71 full-term healthy infants at 18 months of age. We hypothesized that children would differ in gut microbial diversity, enterotypes obtained by Dirichlet multinomial mixture analysis and specific taxa based on their behavioural characteristics. RESULTS: In children dichotomized by behavioural trait performance in above- and below-median groups, weighted Unifrac b-diversity exhibited significant differences in fine motor (FM) activity. Dirichlet multinomial mixture modelling identified two enterotypes strongly associated with FM outcomes. When controlling for maternal pre-gestational BMI and breastfeeding for up to 3 months, the examination of signature taxa in FM groups showed that Turicibacter and Parabacteroides were highly abundant in the below-median FM group, while Collinsella, Coprococcus, Enterococcus, Fusobacterium, Holdemanella, Propionibacterium, Roseburia, Veillonella, an unassigned genus within Veillonellaceae and, interestingly, probiotic Bifidobacterium and Lactobacillus were more abundant in the above-median FM group. CONCLUSIONS: Our results suggest an association between enterotypes and specific genera with FM activity and may represent an opportunity for probiotic interventions relevant to treatment for motor disorders.

The protective effects of yellow tea extract against loperamide-induced constipation in mice

2021 ◽  
Author(s):  
Pei-Qin Cao ◽  
Xiu-Ping Li ◽  
Jian Ou-Yang ◽  
Rong-Gang Jiang ◽  
Fang-Fang Huang ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Protective Effects ◽  
Rrna Gene Sequencing ◽  
Tea Extract

We evaluated the effects of yellow tea extract on relieving constipation induced by loperamide and evaluated the changes of gut microbiota based on 16S rRNA gene sequencing.

scholarly journals Insight Into the Potential Value of Gut Microbial Signatures for Prediction of Gestational Anemia

2021 ◽  
Vol 11 ◽  
Author(s):  
Hongcheng Wei ◽  
Siting Deng ◽  
Yufeng Qin ◽  
Xu Yang ◽  
Ting Chen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Pregnant Women ◽  
Predictive Value ◽  
16S Rrna Gene Sequencing ◽  
Well Being ◽  
Rrna Gene ◽  
Second Trimester ◽  
Healthy Control ◽  
Rrna Gene Sequencing ◽  
Validation Set

The gut microbiota alternations are associated with gestational anemia (GA); however, limited predictive value for the subsequent incidence of anemia in normal gestational women has been obtained. We sought to rigorously characterise gut dysbiosis in subjects with GA and explored the potential predictive value of novel microbial signatures for the risk of developing GA. A prospective cohort of subjects with GA (n = 156) and healthy control (n = 402), all of whom were free of GA in the second trimester, by 16S rRNA gene sequencing was conducted. Microbial signatures altered dramatically in GA compared with healthy control in the second trimester. Megamonas, Veillonella, and Haemophilus were confirmed to show differential abundances in GA after adjusting for covariates. On the contrary, Lachnospiraceae and Blautia were enriched in control. Microbial co-abundance group (CAG) network was constructed. Prospectively, CAG network relatively accurately predicted upcoming GA in normal pregnant women with an AUC of 0.7738 (95%CI: 0.7171, 0.8306) and the performance was further validated in Validation set (0.8223, 95%CI: 0.7573, 0.8874). Overall, our study demonstrated that alterations in the gut microbial community were associated with anemia in pregnancy and microbial signatures could accurately predict the subsequent incidence of anemia in normal pregnant women. Our findings provided new insights into understanding the role of gut microbiota in GA, identifying high-risk individuals, and modulating gut microbiota as a therapeutic target, thus improving quality of life and well-being of women and children.

scholarly journals Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

2021 ◽  
Vol 11 ◽  
Author(s):  
Shiju Xiao ◽  
Guangzhong Zhang ◽  
Chunyan Jiang ◽  
Xin Liu ◽  
Xiaoxu Wang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Target Genes ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Phosphotransferase System ◽  
Microbial Composition ◽  
Kegg Pathways ◽  
Significant Difference ◽  
Rrna Gene Sequencing ◽  
Endothelial Growth Factor

BackgroundIncreasing evidence has shown that alterations in the intestinal microbiota play an important role in the pathogenesis of psoriasis. The existing relevant studies focus on 16S rRNA gene sequencing, but in-depth research on gene functions and comprehensive identification of microbiota is lacking.ObjectivesTo comprehensively identify characteristic gut microbial compositions, genetic functions and relative metabolites of patients with psoriasis and to reveal the potential pathogenesis of psoriasis.MethodsDNA was extracted from the faecal microbiota of 30 psoriatic patients and 15 healthy subjects, and metagenomics sequencing and bioinformatic analyses were performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database, cluster of orthologous groups (COG) annotations, and metabolic analyses were used to indicate relative target genes and pathways to reveal the pathogenesis of psoriasis.ResultsCompared with healthy individuals, the gut microbiota of psoriasis patients displayed an alteration in microbial taxa distribution, but no significant difference in microbial diversity. A distinct gut microbial composition in patients with psoriasis was observed, with an increased abundance of the phyla Firmicutes, Actinobacteria and Verrucomicrobia and genera Faecalibacterium, Bacteroides, Bifidobacterium, Megamonas and Roseburia and a decreased abundance of the phyla Bacteroidetes, Euryarchaeota and Proteobacteria and genera Prevotella, Alistipes, and Eubacterium. A total of 134 COGs were predicted with functional analysis, and 15 KEGG pathways, including lipopolysaccharide (LPS) biosynthesis, WNT signaling, apoptosis, bacterial secretion system, and phosphotransferase system, were significantly enriched in psoriasis patients. Five metabolites, hydrogen sulfide (H2S), isovalerate, isobutyrate, hyaluronan and hemicellulose, were significantly dysregulated in the psoriatic cohort. The dysbiosis of gut microbiota, enriched pathways and dysregulated metabolites are relevant to immune and inflammatory response, apoptosis, the vascular endothelial growth factor (VEGF) signaling pathway, gut-brain axis and brain-skin axis that play important roles in the pathogenesis of psoriasis.ConclusionsA clear dysbiosis was displayed in the gut microbiota profile, genetic functions and relative metabolites of psoriasis patients. This study is beneficial for further understanding the inflammatory pathogenesis of psoriasis and could be used to develop microbiome-based predictions and therapeutic approaches.

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