scholarly journals Clinical and microbiological characteristics of Cryptococcus gattii isolated from 7 hospitals in China

2020 ◽  
Author(s):  
Liang Jin ◽  
Jingrong Cao ◽  
Xinying Xue ◽  
Hua Wu ◽  
Lifeng Wang ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Proteome Analysis ◽  
The United States ◽  
Cryptococcus Gattii ◽  
Differentially Expressed Proteins ◽  
Protein Expression Profile ◽  
Minimal Inhibitory Concentrations ◽  
Microbiological Characteristics ◽  
Significant Difference

Abstract Background: Infection, even outbreak, caused by Cryptococcus gattii (C. gattii ) has been reported in Canada and the United States, but there were sparsely-reported cases of C. gattii in China. Our interest in occurrence, clinical manifestation, laboratory identification and molecular characterization of Chinese C. gattii strains leads us to this research. Results: Out of 254 clinical isolates, initially identified as Cryptococcus neoformans (C. neoformans ), eight strains were re-identified as C. gattii. Multi-locus sequence typing (MLST) showed genotype VGI accounted for the most (6 / 8), the other two strains were genotype VGII（VGIIa and VGIIb respectively）with 3 specific spectra of molecular weight about 4342, 8686, 9611 Dalton by MALDI-TOF MS. The minimal inhibitory concentrations (MICs) of Fluconazole with Yeast one was 2～4 times higher than that with ATB fungus 3 and MICs of antifungal agents against VGII strains were higher than against VGI strains. Comparative proteome analysis showed that 329 and 180 proteins were highly expressed by C. gattii VGI and VGII respectively. The enrichment of differentially expressed proteins was directed to Golgi complex.Conclusions: Infection by C. gattii in China occurred sparsely. Genotype VGI was predominant but VGII was more resistant to antifungal agents. There was significant difference in protein expression profile between isolates of VGI and VGII C. gattii. Key words: Cryptococcus gattii, Genotype, Antifungal agents, Spectrum, differential protein

Clinical and microbiological characteristics of Cryptococcus gattii isolated from China

2020 ◽  
Author(s):  
Liang Jin ◽  
Jingrong Cao ◽  
Xinying Xue ◽  
Hua Wu ◽  
Lifeng Wang ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
The United States ◽  
Cryptococcus Gattii ◽  
Clinical Isolates ◽  
Strain Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Significant Difference ◽  
Tof Ms

Abstract Background: Infection, even outbreak, caused by Cryptococcus gattii (C. gattii ) has been reported in Canada and the United States, but there were sparsely-reported cases of C. gattii in China. Our interest in occurrence, clinical manifestation, laboratory identification and molecular characterization of Chinese C. gattii strains leads us to this research. Methods: A total of 254 clinical isolates primarily identified as Cryptococcus neoformans (C. neoformans ) were collected. VITEK 2 compact, canavanine glycine bromothymol blue (CGB) agar and Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used for strain identification. Multi-locus sequence typing (MLST) was performed for genotyping. Antifungal susceptibility test was carried out with commercial kits of both ATB fungus 3 and Yeast one. Clinical information of patients was reviewed retrospectively. Label-free proteome technique was used to quantitatively analyze the differential proteins of C. gattii. Results: Out of 254 clinical isolates, we identified eight strains as C. gattii. MLST showed genotype VGI accounted for the most (6 / 8), the other two strains were genotype VGII（VGIIa and VGIIb respectively）with 3 specific spectra of molecular weight about 4342, 8686, 9611 Dalton by MALDI-TOF MS. The minimal inhibitory concentrations (MICs) of Fluconazole with Yeast one was 2～4 times higher than that with ATB fungus 3. Higher MICs of antifungal agents were exhibited against VGII strains than against VGI strains. C. gattii genotype VGII and VGI possessed 418 and 774 specific proteins respectively. Comparative proteome analysis showed that 180 proteins were highly expressed in C. gattii VGII and 329 proteins were highly expressed in C. gattii VGI. The enrichment of differentially expressed proteins between VGII and VGI was directed to Golgi complex.Conclusions: Infection by C. gattii in China might have been underestimated because of initial mis-identification. Genotype VGI was predominant but VGII was more resistant to antifungal agents. There was significant difference in protein expression profile between VGII and VGI C. gattii.

scholarly journals Clinical and microbiological characteristics of Cryptococcus gattii isolated from 7 hospitals in China

2020 ◽  
Author(s):  
Liang Jin ◽  
Jingrong Cao ◽  
Xinying Xue ◽  
Hua Wu ◽  
Lifeng Wang ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
The United States ◽  
Cryptococcus Gattii ◽  
Clinical Isolates ◽  
Strain Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Significant Difference ◽  
Tof Ms

Abstract Background: Infection, even outbreak, caused by Cryptococcus gattii (C. gattii ) has been reported in Canada and the United States, but there were sparsely-reported cases of C. gattii in China. Our interest in occurrence, clinical manifestation, laboratory identification and molecular characterization of Chinese C. gattii strains leads us to this research.Methods: A total of 254 clinical isolates primarily identified as Cryptococcus neoformans (C. neoformans ) were collected. VITEK 2 compact, canavanine glycine bromothymol blue (CGB) agar and Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used for strain identification. Multi-locus sequence typing (MLST) was performed for genotyping. Antifungal susceptibility test was carried out with commercial kits of both ATB fungus 3 and Yeast one. Clinical information of patients was reviewed retrospectively. Label-free proteome technique was used to quantitatively analyze the differential proteins of C. gattii. Results: Out of 254 clinical isolates, we identified eight strains as C. gattii. MLST showed genotype VGI accounted for the most (6 / 8), the other two strains were genotype VGII（VGIIa and VGIIb respectively）with 3 specific spectra of molecular weight about 4342, 8686, 9611 Dalton by MALDI-TOF MS. The minimal inhibitory concentrations (MICs) of Fluconazole with Yeast one was 2～4 times higher than that with ATB fungus 3. Higher MICs of antifungal agents were exhibited against VGII strains than against VGI strains. C. gattii genotype VGI and VGII possessed 418 and 774 specific proteins respectively. Comparative proteome analysis showed that 329 and 180 proteins were highly expressed in C. gattii VGI and VGII. The enrichment of differentially expressed proteins was directed to Golgi complex.Conclusions: Infection by C. gattii in China might have been underestimated because of initial mis-identification. Genotype VGI was predominant but VGII was more resistant to antifungal agents. There was significant difference in protein expression profile between VGI and VGII C. gattii.

scholarly journals Characterization of Environmental Sources of the Human and Animal Pathogen Cryptococcus gattii in British Columbia, Canada, and the Pacific Northwest of the United States

2006 ◽  
Vol 73 (5) ◽  
pp. 1433-1443 ◽  
Author(s):  
Sarah E. Kidd ◽  
Yat Chow ◽  
Sunny Mak ◽  
Paxton J. Bach ◽  
Huiming Chen ◽  
...  
Keyword(s):  
British Columbia ◽  
Pacific Northwest ◽  
Sampling Site ◽  
The United States ◽  
Cryptococcus Gattii ◽  
Environmental Distribution ◽  
The Pacific ◽  
Genetic Subtype ◽  
The Central Nervous System

ABSTRACT Cryptococcus gattii has recently emerged as a primary pathogen of humans and wild and domesticated animals in British Columbia, particularly on Vancouver Island. C. gattii infections are typically infections of the pulmonary and/or the central nervous system, and the incidence of infection in British Columbia is currently the highest reported globally. Prior to this emergence, the environmental distribution of and the extent of colonization by C. gattii in British Columbia were unknown. We characterized the environmental sources and potential determinants of colonization in British Columbia. C. gattii was isolated from tree surfaces, soil, air, freshwater, and seawater, and no seasonal prevalence was observed. The C. gattii concentrations in air samples were significantly higher during the warm, dry summer months, although potentially infectious propagules (<3.3 μm in diameter) were present throughout the year. Positive samples were obtained from many different areas of British Columbia, and some locations were colonization “hot spots.” C. gattii was generally isolated from acidic soil, and geographic differences in soil pH may influence the extent of colonization. C. gattii soil colonization also was associated with low moisture and low organic carbon contents. Most of the C. gattii isolates recovered belonged to the VGIIa genetic subtype; however, sympatric colonization by the VGIIb strain was observed at most locations. At one sampling site, VGIIa, VGIIb, VGI, and the Cryptococcus neoformans serotype AD hybrid all were coisolated. Our findings indicate extensive colonization by C. gattii within British Columbia and highlight an expansion of the ecological niche of this pathogen.

scholarly journals Variation in pathogen aggression and cultivar performance against Sclerotinia sclerotiorum in soybean and dry bean from Brazil and the U.S.

2018 ◽  
Author(s):  
Thomas J. J. Miorini ◽  
Zhian N. Kamvar ◽  
Rebecca Higgins ◽  
Carlos G. Raetano ◽  
James R. Steadman ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
The United States ◽  
Isolated Populations ◽  
Dry Bean ◽  
Bean Cultivar ◽  
Soybean Cultivars ◽  
Significant Difference ◽  
Management Recommendations ◽  
The U.S

Sclerotinia sclerotiorum (Lib.) de Bary is an important yield-reducing disease in the United States and Brazil with a diverse host range, including dry bean and soybean. Characterization of both the physiological resistance of commercial cultivars to S. sclerotiorum and the range of aggressiveness among S. sclerotiorum isolates collected from locations where these cultivars are to be deployed, provides important information for making management recommendations. To address this in the present study, we first sought to characterize the range of S. sclerotiorum aggressiveness on soybean and dry bean, using a selection of isolates from Brazil and the U.S. Our second objective was to evaluate the performance of dry bean and soybean cultivars that were developed in Brazil. Eighty-seven isolates of S. sclerotiorum were collected from soybean crops from the U.S. and Brazil and used in this project. U.S. soybean cultivar Dassel and Brazilian dry bean cultivar IAC Alvorada were evaluated using a detached leaf bioassay (DLB) with 65 and 28 isolates respectively. For the straw test, U.S. dry bean cultivar G122 and Brazilian dry bean cultivar IAC Alvorada were inoculated with 32 and 28 isolates respectively. Results showed a significant difference among isolates (P < 0.001). After evaluating 23 Brazilian dry bean cultivars, results of the DLB and straw test showed IAC Diplomata and IPR Tangará were more tolerant to S. sclerotiorum. Among the 11 Brazilian soybean cultivars, M5410 and M6410 were found to be less susceptible to S. sclerotiorum. Collectively, results of this study are important for improving our understanding of variation in pathogen aggressiveness from geographically isolated populations and in identifying cultivars that are likely to have partial resistance.

scholarly journals Clinical and Microbiological Characterization of Carbapenem-Resistant Enterobacteriales: A Prospective Cohort Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Qiuxia Lin ◽  
Menglu Wu ◽  
Hanbing Yu ◽  
Xiaojiong Jia ◽  
Hua Zou ◽  
...  
Keyword(s):  
Cohort Study ◽  
Klebsiella Pneumoniae ◽  
Epidemiological Data ◽  
Resistance Mechanisms ◽  
Strain Identification ◽  
Carbapenem Resistant ◽  
Microbiological Characteristics ◽  
Significant Difference ◽  
Poor Outcomes

Aim: We aim to depict the clinicoepidemiological and molecular information of carbapenem-resistant Enterobacteriales (CRE) in Chongqing, China.Methods: We performed a prospective, observational cohort study, recruiting inpatients diagnosed with CRE infections from June 1, 2018, to December 31, 2019. We carried out strain identification and molecular characterization of CRE. eBURST analysis was conducted to assess the relationships among the different isolates on the basis of their sequence types (STs) and associated epidemiological data using PHYLOViZ. Clinical parameters were compared between the carbapenemase-producing Enterobacteriales (CPE) and non-CPE group.Findings: 128 unique CRE isolates from 128 patients were collected during the study period: 69 (53.9%) CPE and 59 (46.1%) non-CPE. The majority of CPE isolates were blaKPC-2 (56.5%), followed by blaNDM (39.1%) and blaIMP (5.8%). Klebsiella pneumoniae carbapenemase (KPC)–producing clonal group 11 Klebsiella pneumoniae (K. pneumoniae) was the most common CPE. Antibiotic resistance was more frequent in the CPE group than in the non-CPE group. Independent predictors for CPE infection were ICU admission and hepatobiliary system diseases. Although, there was no significant difference in desirability of outcome ranking (DOOR) outcomes between the two groups. At 30 days after index culture, 35 (27.3% ) of these patients had died.Conclusion: CRE infections were related to high mortality and poor outcomes, regardless of CRE subgroups. CPE were associated with prolonged ICU stays and had different clinical and microbiological characteristics than non-CPE. The identification of CPE/non-CPE and CRE resistance mechanisms is essential for better guidance of the clinical administration of patients with CRE infections.

scholarly journals Variation in pathogen aggression and cultivar performance against Sclerotinia sclerotiorum in soybean and dry bean from Brazil and the U.S.

2018 ◽  
Author(s):  
Thomas J. J. Miorini ◽  
Zhian N. Kamvar ◽  
Rebecca Higgins ◽  
Carlos G. Raetano ◽  
James R. Steadman ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
The United States ◽  
Isolated Populations ◽  
Dry Bean ◽  
Bean Cultivar ◽  
Soybean Cultivars ◽  
Significant Difference ◽  
Management Recommendations ◽  
The U.S

Sclerotinia sclerotiorum (Lib.) de Bary is an important yield-reducing disease in the United States and Brazil with a diverse host range, including dry bean and soybean. Characterization of both the physiological resistance of commercial cultivars to S. sclerotiorum and the range of aggressiveness among S. sclerotiorum isolates collected from locations where these cultivars are to be deployed, provides important information for making management recommendations. To address this in the present study, we first sought to characterize the range of S. sclerotiorum aggressiveness on soybean and dry bean, using a selection of isolates from Brazil and the U.S. Our second objective was to evaluate the performance of dry bean and soybean cultivars that were developed in Brazil. Eighty-seven isolates of S. sclerotiorum were collected from soybean crops from the U.S. and Brazil and used in this project. U.S. soybean cultivar Dassel and Brazilian dry bean cultivar IAC Alvorada were evaluated using a detached leaf bioassay (DLB) with 65 and 28 isolates respectively. For the straw test, U.S. dry bean cultivar G122 and Brazilian dry bean cultivar IAC Alvorada were inoculated with 32 and 28 isolates respectively. Results showed a significant difference among isolates (P < 0.001). After evaluating 23 Brazilian dry bean cultivars, results of the DLB and straw test showed IAC Diplomata and IPR Tangará were more tolerant to S. sclerotiorum. Among the 11 Brazilian soybean cultivars, M5410 and M6410 were found to be less susceptible to S. sclerotiorum. Collectively, results of this study are important for improving our understanding of variation in pathogen aggressiveness from geographically isolated populations and in identifying cultivars that are likely to have partial resistance.

Characterization of stilbenoids from the stem bark of Norway spruce (Picea abies) as antifungal agents

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
J Gabaston ◽  
T Richard ◽  
B Biais ◽  
P Waffo-Teguo ◽  
MF Corio-Costet ◽  
...  
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Antifungal Agents ◽  
Stem Bark

Caracterização da Peneira Média em Clones de Coffea canephora

REVISTA FIMCA ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 28-31
Author(s):  
Darlan Darlan Sanches Barbosa Alves ◽  
Victor Mouzinho Spinelli ◽  
Marcos Santana Moraes ◽  
Carolina Augusto De Souza ◽  
Rodrigo da Silva Ribeiro ◽  
...  
Keyword(s):  
Genetic Improvement ◽  
Block Design ◽  
Coffea Canephora ◽  
Coffee Production ◽  
Randomized Block Design ◽  
Significant Difference ◽  
The Mean ◽  
Four Blocks ◽  
Selection Of

Introdução: O estado de Rondônia se destaca como tradicional produtor de café, sendo o segundo maior produtor brasileiro de C. canephora. No melhoramento genético de C. canephora, a seleção de plantas de elevada peneira média está associada à bebida de qualidade superior. Objetivos: O objetivo desse estudo foi avaliar a variabilidade genética de clones de C. canephora para o tamanho dos grãos, mensurado a partir da avaliação da peneira média (PM). Materiais e Métodos: Para isso, foi conduzido ao longo de dois anos agrícolas experimento no campo experimental da Embrapa no município de Ouro Preto do Oeste-RO, para a avaliação da peneira média de 130 genótipos (clones) com características das variedades botânicas Conilon, Robusta e híbridos intervarietais. O delineamento experimental utilizado foi de blocos ao acaso, com quatro repetições de quatro plantas por parcela. Resultados: Não houve resultados significativos para a interação clones X anos, indicando uma maior consistência no comportamento das plantas ao longo do tempo. Porém foram observadas diferenças significativas para o tamanho dos grãos entre os genótipos avaliados, possibilitando selecionar genótipos superiores. Conclusão: Os genótipos agruparam-se em cinco classes de acordo com o teste de média, subsidiando a caracterização de um gradiente de variabilidade da característica avaliada ABSTRACTIntroduction: Coffea canephora accounts for approximately 35% of the world's coffee production. The state of Rondônia stands out as a traditional coffee producer, being the second largest Brazilian producer of C. canephora. In the classical genetic improvement of C. anephora, the selection of plants of high average sieve is associated with a drink of superior quality. Objectives: The objective of this udy was to evaluate the genetic variability of Coffea canephora clones for the agronomic medium sieve (PM). Materials and Methods: The experiment was conducted in the experimental field of Embrapa, municipality of OuroPreto do Oeste-RO, located at coordinates 10º44'53 "S and 62º12'57". One hundred thirty genotypes (clones) of botanical characteristics Conilon, Robusta and intervarietal hybrids were evaluated in the agricultural years 2013-2014 and 2014-2015. The experimental design was a randomized block design with four blocks and four plants per plot, spacing 3.5 x 1.5 meters between plants. Results: Significant difference was found for the grain size. According to the F test, at 5% probability, the genotypes were grouped into five classes according to the mean test. Conclusion: The results obtained subsidized the characterization of a variability gradient of the evaluated trait.

scholarly journals Protective Effectiveness of Long-Lasting Permethrin Impregnated Clothing Against Tick Bites in an Endemic Lyme Disease Setting: A Randomized Control Trial Among Outdoor Workers

2020 ◽  
Vol 57 (5) ◽  
pp. 1532-1538
Author(s):  
Cedar Mitchell ◽  
Megan Dyer ◽  
Feng-Chang Lin ◽  
Natalie Bowman ◽  
Thomas Mather ◽  
...  
Keyword(s):  
United States ◽  
Rhode Island ◽  
The United States ◽  
Amblyomma Americanum ◽  
Adverse Outcomes ◽  
Control Group ◽  
Tick Bites ◽  
Outdoor Workers ◽  
Significant Difference ◽  
Protective Effectiveness

Abstract Tick-borne diseases are a growing threat to public health in the United States, especially among outdoor workers who experience high occupational exposure to ticks. Long-lasting permethrin-impregnated clothing has demonstrated high initial protection against bites from blacklegged ticks, Ixodes scapularis Say (Acari: Ixodidae), in laboratory settings, and sustained protection against bites from the lone star tick, Amblyomma americanum (L.) (Acari: Ixodidae), in field tests. However, long-lasting permethrin impregnation of clothing has not been field tested among outdoor workers who are frequently exposed to blacklegged ticks. We conducted a 2-yr randomized, placebo-controlled, double-blinded trial among 82 outdoor workers in Rhode Island and southern Massachusetts. Participants in the treatment arm wore factory-impregnated permethrin clothing, and the control group wore sham-treated clothing. Outdoor working hours, tick encounters, and bites were recorded weekly to assess protective effectiveness of long-lasting permethrin-impregnated garments. Factory-impregnated clothing significantly reduced tick bites by 65% in the first study year and by 50% in the second year for a 2-yr protective effect of 58%. No significant difference in other tick bite prevention method utilization occurred between treatment and control groups, and no treatment-related adverse outcomes were reported. Factory permethrin impregnation of clothing is safe and effective for the prevention of tick bites among outdoor workers whose primary exposure is to blacklegged ticks in the northeastern United States.

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