Bifidobacteriaceae family diversity in gut microbiota of patients with renal failure
Abstract Background: Bifidobacteriaceae family are belonged to the gut microbiota that could exhibit probiotic or health promoting effects on the host. Several studies suggested that gut microbiota are quantitative and qualitative altered in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). The present study was aimed to assess the members of Bifidobacteriaceae family in fecal samples of patients with CKD and ESRD in compared to non-CKD/ESRD patients to find any changes of their counts in these patients.Methods: Twenty fresh fecal samples of patients with CKD/ESRD and twenty from non-CKD/ESRD patients were included. The whole DNA of fecal samples were extracted and the gut microbiota composition was analyzed by next generation sequencing (NGS) method.Results: Total 651 strains were identified from 40 fecal samples, which 8 (1.23%) strains were identified as family Bifidobacteriaceae. The most abundance species in both control and disease group were Bifidobacterium adolescentis (2.10% ± 1.05% vs. 1.98% ± 1.53%, respectively) and the lowest abundance species in disease group was Bifidobacterium animalis subsp. lactis (0.0007% ± 0.0009%).Conclusions: There was no significant differentiation in the abundance of various species between disease group and control group (p<0.05). This study has confirmed that the members of Bifidobacteriaceae family are not alters in patients with CKD/ESRD.