scholarly journals A Novel Insight Into Mechanism of Derangement of Coagulation Balance: Interactions of Quantum Dots with Coagulation-Related Proteins

2021 ◽  
Author(s):  
Lingyan Zhang ◽  
Yingting Wu ◽  
Xingling Luo ◽  
Tianjiang Jia ◽  
Kexin Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Active Sites ◽  
Molecular Level ◽  
Comprehensive Evaluation ◽  
Circulatory System ◽  
Electronic Products ◽  
Protein Conjugates ◽  
Related Proteins ◽  
Insight Into ◽  
Biosafety Evaluation

Abstract Background Quantum dots (QDs) have gained increased attention for their extensive biomedical and electronic products applications. Due to the high priority of QDs in contacting the circulatory system, understanding the hemocompatibility of QDs is one of the most important aspects for their biosafety evaluation. Thus far, the effect of QDs on coagulation balance haven’t been fully understood, and limited studies also have yet elucidated the potential mechanism from the perspective of interaction of QDs with coagulation-related proteins. Results QDs induced the derangement of coagulation balance by prolonging the activated partial thromboplastin time and prothrombin time as well as changing the expression levels of coagulation and fibrinolytic factors. The contact of QDs with PTM (prothrombin), PLG (plasminogen) and FIB (fibrinogen) which are primary coagulation-related proteins in the coagulation and fibrinolytic systems formed QDs-protein conjugates through hydrogen-bonding and hydrophobic interaction. The affinity of proteins with QDs followed the order of PTM>PLG>FIB, and was larger with CdTe/ZnS QDs than CdTe QDs. Binding with QDs not only induced static fluorescence quenching of PTM, PLG and FIB, but also altered their conformational structures. The binding of QDs to the active sites of PTM, PLG and FIB may promote the activation of proteins, thus interfering the hemostasis and fibrinolysis processes. Conclusions The interactions of QDs with PTM, PLG and FIB may be key contributors for interference of coagulation balance, that is helpful to achieve a reliable and comprehensive evaluation on the potential biological influence of QDs from the molecular level.

A comparative proteomic analysis for adventitious root formation in lotus root (Nelumbo nucifera Gaertn)

2017 ◽  
Vol 72 (5-6) ◽  
pp. 181-196
Author(s):  
Cheng Libao ◽  
Jiang Runzhi ◽  
Yang Mengli ◽  
Li Liangjun ◽  
Li Shuyan
Keyword(s):  
Molecular Mechanisms ◽  
Root Formation ◽  
Molecular Level ◽  
Adventitious Root Formation ◽  
Lotus Root ◽  
Liquid Chromatography Tandem Mass ◽  
Three Stages ◽  
Isobaric Tags ◽  
Related Proteins ◽  
Insight Into

AbstractAdventitious roots (ARs) directly affect lotus seedling growth and product quality because principal root is not well developed. However, the details of AR formation at the molecular level have not been determined in lotus. Therefore, three stages were chosen to identify the change of proteins abundant during rhizome formation, using isobaric tags for relative and absolute quantization coupled with liquid chromatography–tandem mass spectrometry to gain insight into the molecular mechanisms involved in AR formation. We totally obtained 323,375 spectra during AR formation. After filtering to eliminate low-scoring spectra, 66,943 spectra, including 53,106 unique spectra, were identified. These unique spectra matched 28,905 peptides, including 24,992 unique peptides, which were assembled into 6686 proteins. In the C0/C1 and C1/C2 stages, 66 and 32 proteins showed enhanced abundance, and 173 and 73 proteins showed decreased abundance, respectively. Seventeen important AR formation-related proteins from the three stages were identified, and the expressions of nine genes from the above-identified proteins were assessed by qRT-PCR. This article provides a comprehensive understanding of the changes in metabolism during AR formation, and is helpful to accelerate the progress of breeding in fulture in lotus root.

scholarly journals Adipo-Derived Stem Cell Features and MCF-7

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1754
Author(s):  
Giuseppe Garroni ◽  
Francesca Balzano ◽  
Sara Cruciani ◽  
Renzo Pala ◽  
Donatella Coradduzza ◽  
...  
Keyword(s):  
Stem Cell ◽  
Molecular Level ◽  
Cancer Cell Line ◽  
Human Adipose Tissue ◽  
Cell Cycle Regulators ◽  
Culturing Conditions ◽  
And Migration ◽  
Related Proteins ◽  
Mcf 7

Human adipose tissue-derived stem cells (hADSCs) are highly suitable for regeneration therapies being easily collected and propagated in vitro. The effects of different external factors and culturing conditions are able to affect hADSC proliferation, senescence, differentiation, and migration, even at the molecular level. In the present paper, we exposed hADSCs to an exhausted medium from the breast cancer cell line (MCF-7) to evaluate whether the soluble factors released by these cells may be able to induce changes in stem cell behavior. In particular, we investigated the expression of stemness-related genes (OCT4; Sox 2; Nanog), the cell-cycle regulators p21 (WAF1/CIP1) p53, epigenetic markers (DNMT1 and Sirt1), and autophagy-related proteins. From our results, we can infer that the exhausted medium from MCF-7 is able to influence the hADSCs behavior increasing the expression of stemness-related genes, cell proliferation, and autophagy. Polyamines detectable in MCF-7 exhausted medium could be related to the higher proliferation capability observed in hADSCs, suggesting direct crosstalk between these molecules and the observed changes in stem cell potency.

Mechanism Insight into Rapid Photodriven Sterilization Based on Silver Bismuth Sulfide Quantum Dots

2021 ◽  
Author(s):  
Shengwu Wen ◽  
Tianhua Wu ◽  
Hui Long ◽  
Liying Ke ◽  
Suiping Deng ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Bismuth Sulfide ◽  
Insight Into

scholarly journals Noni fruit’s water spot appearance on the second day of harvest: a trade-off between resistance and energy

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tian Wu ◽  
Danyan Hu ◽  
Qingfen Wang
Keyword(s):  
Statistical Analysis ◽  
Molecular Level ◽  
Morinda Citrifolia ◽  
Transcriptome Data ◽  
Trade Off ◽  
Kegg Pathways ◽  
Available Energy ◽  
Climacteric Fruit ◽  
The Relationship ◽  
Insight Into

Abstract Background Noni (Morinda citrifolia Linn.) is a tropical tree that bears climacteric fruit. Previous observations and research have shown that the second day (2 d) after harvest is the most important demarcation point when the fruit has the same appearance as the freshly picked fruit (0 d); however, they are beginning to become water spot appearance. We performed a conjoint analysis of metabolome and transcriptome data for noni fruit of 0 d and 2 d to reveal what happened to the fruit at the molecular level. Genes and metabolites were annotated to KEGG pathways and the co-annotated KEGG pathways were used as a statistical analysis. Results We found 25 pathways that were significantly altered at both metabolic and transcriptional levels, including a total of 285 differentially expressed genes (DEGs) and 11 differential metabolites through an integrative analysis of transcriptomics and metabolomics. The energy metabolism and pathways originating from phenylalanine were disturbed the most. The upregulated resistance metabolites and genes implied the increase of resistance and energy consumption in the postharvest noni fruit. Most genes involved in glycolysis were downregulated, further limiting the available energy. This lack of energy led noni fruit to water spot appearance, a prelude to softening. The metabolites and genes related to the resistance and energy interacted and restricted each other to keep noni fruit seemingly hard within two days after harvest, but actually the softening was already unstoppable. Conclusions This study provides a new insight into the relationship between the metabolites and genes of noni fruit, as well as a foundation for further clarification of the post-ripening mechanism in noni fruit.

scholarly journals Cisplatin under oriented external electric fields: A deeper insight into electrochemotherapy at the molecular level

2020 ◽  
Author(s):  
Li Zhang ◽  
Ya‐Ling Ye ◽  
Xiao‐Ling Zhang ◽  
Xiang‐Hui Li ◽  
Qiao‐Hong Chen ◽  
...  
Keyword(s):  
Electric Fields ◽  
Molecular Level ◽  
External Electric Fields ◽  
Insight Into

scholarly journals A New Saccharomyces cerevisiae Strain with a Mutant Smt3-Deconjugating Ulp1 Protein Is Affected in DNA Replication and Requires Srs2 and Homologous Recombination for Its Viability

2004 ◽  
Vol 24 (12) ◽  
pp. 5130-5143 ◽  
Author(s):  
Christine Soustelle ◽  
Laurence Vernis ◽  
Karine Fréon ◽  
Anne Reynaud-Angelin ◽  
Roland Chanet ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Homologous Recombination ◽  
Nonhomologous End Joining ◽  
End Joining ◽  
Recombination Mechanism ◽  
Growth Defects ◽  
Protein Conjugates ◽  
Synthetically Lethal ◽  
Mutant Cells ◽  
Insight Into

ABSTRACT The Saccharomyces cerevisiae Srs2 protein is involved in DNA repair and recombination. In order to gain better insight into the roles of Srs2, we performed a screen to identify mutations that are synthetically lethal with an srs2 deletion. One of them is a mutated allele of the ULP1 gene that encodes a protease specifically cleaving Smt3-protein conjugates. This allele, ulp1-I615N, is responsible for an accumulation of Smt3-conjugated proteins. The mutant is unable to grow at 37°C. At permissive temperatures, it still shows severe growth defects together with a strong hyperrecombination phenotype and is impaired in meiosis. Genetic interactions between ulp1 and mutations that affect different repair pathways indicated that the RAD51-dependent homologous recombination mechanism, but not excision resynthesis, translesion synthesis, or nonhomologous end-joining processes, is required for the viability of the mutant. Thus, both Srs2, believed to negatively control homologous recombination, and the process of recombination per se are essential for the viability of the ulp1 mutant. Upon replication, mutant cells accumulate single-stranded DNA interruptions. These structures are believed to generate different recombination intermediates. Some of them are fixed by recombination, and others require Srs2 to be reversed and fixed by an alternate pathway.

Anodic stripping voltammetry of anti-Vi antibody functionalized CdTe quantum dots for the specific monitoring of Salmonella enterica serovar Typhi

RSC Advances ◽  
2015 ◽  
Vol 5 (107) ◽  
pp. 88234-88240 ◽  
Author(s):  
Satish K. Pandey ◽  
Praveen Rishi ◽  
C. Raman Suri ◽  
Aaydha C. Vinayaka
Keyword(s):  
Quantum Dots ◽  
Capsular Polysaccharide ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Cdte Quantum Dots ◽  
Salmonella Enterica Serovar Typhi ◽  
Novel Approach ◽  
Anodic Stripping ◽  
Vi Capsular Polysaccharide ◽  
Insight Into

CdTe QD based stripping voltammetry for Vi capsular polysaccharide detection. The technique has provided an insight into the competence of CdTe QD and GNP immuno-conjugates. This is a novel approach to characterize the efficiency of immuno-conjugates of QDs and GNPs.

Highly Efficient Proton-Assisted Photocatalytic CO2 Reduction over 3-Mercaptopropionic Acid-Capped CdS Quantum Dots

2021 ◽  
Author(s):  
Duc-Trung Nguyen ◽  
Anis Chouat ◽  
Trong-On Do
Keyword(s):  
Quantum Dots ◽  
Active Sites ◽  
Co2 Reduction ◽  
Cds Quantum Dots ◽  
Capping Agents ◽  
Mercaptopropionic Acid ◽  
Highly Efficient

Herein, we demonstrate that 3-mercaptopropionate capping agents on CdS quantum dots' surface could serve as proton shutters and localize protons near the active sites toward an efficient photocatalytic CO2 reduction....

scholarly journals Insight into Inhibitor Binding in the Eukaryotic Proteasome: Computations of the 20S CP

2018 ◽  
Vol 19 (12) ◽  
pp. 3858
Author(s):  
Milan Hodošček ◽  
Nadia Elghobashi-Meinhardt
Keyword(s):  
Electrostatic Interactions ◽  
Active Sites ◽  
Sampling Period ◽  
Md Simulations ◽  
Structural Features ◽  
Relaxation Dynamics ◽  
Inhibitor Binding ◽  
Computational Analyses ◽  
Insight Into ◽  
Proteolytic Chamber

A combination of molecular dynamics (MD) simulations and computational analyses uncovers structural features that may influence substrate passage and exposure to the active sites within the proteolytic chamber of the 20S proteasome core particle (CP). MD simulations of the CP reveal relaxation dynamics in which the CP slowly contracts over the 54 ns sampling period. MD simulations of the SyringolinA (SylA) inhibitor within the proteolytic B 1 ring chamber of the CP indicate that favorable van der Waals and electrostatic interactions account for the predominant association of the inhibitor with the walls of the proteolytic chamber. The time scale required for the inhibitor to travel from the center of the proteolytic chamber to the chamber wall is on the order of 4 ns, accompanied by an average energetic stabilization of approximately −20 kcal/mol.

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