Metabolic Response of Larix Olgensis A. Henry to Polyethylene Glycol-simulated Drought Stress

Abstract BackgroundDrought stress in trees limits their growth, survival, and productivity, and it negatively affects the afforestation survival rate. Molecular responses to drought stress have been extensively studied in broad-leaved species, but studies on coniferous species are limited.ResultsOur study focused on the molecular responses to drought stress in a coniferous species, Larix olgensis A. Henry. Drought stress was simulated in one-year-old seedlings using 25% polyethylene glycol 6000. The drought stress response in these seedlings was assessed by analyzing select biochemical parameters, along with gene expression and metabolite profiles. The soluble protein content, peroxidase activity, and malondialdehyde content of L. olgensis were significantly changed during drought stress. Quantitative gene expression analysis identified a total of 8172 differentially expressed genes in seedlings processed after 24 h, 48 h, and 96 h of drought stress treatment. Compared with the gene expression profile of the untreated control, the number of up-regulated genes was higher than that of down-regulated genes, indicating that L. olgensis mainly responded to drought stress through positive regulation. Metabolite analysis of the control and stress-treated samples showed that under drought stress, the increased abundance of linoleic acid was the highest among up-regulated metabolites, which also included some saccharides. A combined analysis of the transcriptome and metabolome revealed that genes dominating the differential expression profile were involved in glutathione metabolism, galactose metabolism, and starch and sucrose metabolism. Moreover, the relative abundance of specific metabolites of these pathways was also altered. Thus, our results indicated that L. olgensis prevented free radical-induced damage through glutathione metabolism and responded to drought through sugar accumulation.ConclusionsThe soluble protein content, peroxidase activity, and malondialdehyde content of L. olgensis were significantly changed during drought stress. A total of 8172 differentially expressed genes in seedlings processed after drought stress treatment. A combined analysis of the transcriptome and metabolome revealed that genes dominating the differential expression profile were involved in glutathione metabolism, galactose metabolism, and starch and sucrose metabolism. Our results indicated that L. olgensis prevented free radical-induced damage through glutathione metabolism and responded to drought through sugar accumulation.

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Glutathione, carbohydrate and other metabolites of Larix olgensis A. Henry reponse to polyethylene glycol-simulated drought stress

PLoS ONE ◽

10.1371/journal.pone.0253780 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0253780

Author(s):

Lei Zhang ◽

Shanshan Yan ◽

Sufang Zhang ◽

Pingyu Yan ◽

Junhui Wang ◽

...

Keyword(s):

Gene Expression ◽

Polyethylene Glycol ◽

Drought Stress ◽

Expression Profile ◽

Glutathione Metabolism ◽

Sugar Accumulation ◽

Larix Olgensis ◽

Galactose Metabolism ◽

Differential Expression Profile ◽

One Year

Drought stress in trees limits their growth, survival, and productivity and it negatively affects the afforestation survival rate. Our study focused on the molecular responses to drought stress in a coniferous species Larix olgensis A. Henry. Drought stress was simulated in one-year-old seedlings using 25% polyethylene glycol 6000. The drought stress response in these seedlings was assessed by analyzing select biochemical parameters, along with gene expression and metabolite profiles. The soluble protein content, peroxidase activity, and malondialdehyde content of L. olgensis were significantly changed during drought stress. Quantitative gene expression analysis identified a total of 8172 differentially expressed genes in seedlings processed after 24 h, 48 h, and 96 h of drought stress treatment. Compared with the gene expression profile of the untreated control, the number of up-regulated genes was higher than that of down-regulated genes, indicating that L. olgensis mainly responded to drought stress through positive regulation. Metabolite analysis of the control and stress-treated samples showed that under drought stress, the increased abundance of linoleic acid was the highest among up-regulated metabolites, which also included some saccharides. A combined analysis of the transcriptome and metabolome revealed that genes dominating the differential expression profile were involved in glutathione metabolism, galactose metabolism, and starch and sucrose metabolism. Moreover, the relative abundance of specific metabolites of these pathways was also altered. Thus, our results indicated that L. olgensis prevented free radical-induced damage through glutathione metabolism and responded to drought through sugar accumulation.

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Gene Expression Profile of Acute Myeloid Leukemia (AML) with t(8;16)(p11;p13) and MYST3/CREBBP Rearrangement.

Blood ◽

10.1182/blood.v104.11.2054.2054 ◽

2004 ◽

Vol 104 (11) ◽

pp. 2054-2054

Author(s):

Mireia Camos ◽

Jordi Esteve ◽

Pedro Jares ◽

Maria Rozman ◽

Dolors Colomer ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profile ◽

Expression Profile ◽

De Novo ◽

Normal Karyotype ◽

Genetic Profile ◽

Transferase Activity ◽

Internal Tandem Duplication ◽

Its Gene ◽

Differential Expression Profile

Abstract Translocation t(8;16)(p11;p13) is an infrequent chromosomal abnormality in de novo and secondary AML cases, leading to the fusion of MYST3 (MOZ) and CREBBP (CBP) genes, both of them harboring histone lysine acetyl-transferase activity. This AML variety displays specific clinical and biological features, although its gene expression profile is currently unknown. In this study, the genetic signature of AML cases with MYST3/CREBBP rearrangement was compared with the genetic profile of other well-defined AML subtypes. Genotypic analyses using oligonucleotide U133A arrays (Affymetrix) were performed on RNA of 19 AML samples, including t(8;16)-AML (n=3), t(15;17) (n=3), t(8;21) (n=2), inv(16)/t(16;16) (n=3), t(9;11) with AF9/MLL rearrangement (n=2), 3 cases with normal karyotype and flt-3 internal tandem duplication (flt-3 ITD), the three remaining samples corresponding to monocytic cases (M4/M5) without MLL rearrangement nor flt-3 ITD. After unsupervised analysis, cases of AML with t(8;16) clustered together, displaying a differential expression profile. Supervised analysis allowed the identification of the top 53 up-regulated and 28 down-regulated genes. Among the set of genes overexpressed, genes involved in chromatin remodelling and transcription (HOXA9, HOXA10, MEIS1, CHD3, SATB1) and protooncogenes (RET, flt-3, LMO2) were identified. In contrast, CREBBP gene and several members of the JAK-STAT pathway (STAT3, STAT5B, JAK2) were underexpressed. Interestingly, overexpression of multiple homeobox genes was detected in flt-3 ITD cases, some of them as a distinctive finding (HOXA2, HOXA3, HOXB6), and others (HOXA9, HOXA10, MEIS1) were found to be highly expressed in MYST3/CREBBP and MLL-rearranged samples. In conclusion, AML with t(8;16) and MYST3/CREBBP rearrangement shows a distinctive gene expression profile, with some similarities with MLL rearranged leukemias and flt-3 ITD AML cases, thus suggesting a partially common leukemogenic pathway.

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Melatonin Alleviates Drought Stress by a Non-Enzymatic and Enzymatic Antioxidative System in Kiwifruit Seedlings

International Journal of Molecular Sciences ◽

10.3390/ijms21030852 ◽

2020 ◽

Vol 21 (3) ◽

pp. 852 ◽

Cited By ~ 3

Author(s):

Hui Xia ◽

Zhiyou Ni ◽

Rongping Hu ◽

Lijin Lin ◽

Honghong Deng ◽

...

Keyword(s):

Gene Expression ◽

Ascorbic Acid ◽

Drought Stress ◽

Carotenoid Biosynthesis ◽

Practical Method ◽

Expression Level ◽

Glutathione Metabolism ◽

Protective Enzyme ◽

Metabolic Gene Expression ◽

Protective Enzyme System

Although melatonin was affirmed to alleviate drought stress in various plant species, the mechanism in kiwifruit remains to be elucidated. In this study, the transcriptomes of kiwifruit leaves under control (CK), DR (drought stress), and MTDR (drought plus melatonin) treatments were evaluated. After comparisons of the gene expression between DR and MTDR, the differentially expressed genes (DEGs) were screened. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated three significant pathways, which were mainly involved in the glutathione metabolism, ascorbate and aldarate metabolism, and carotenoid metabolism. Therefore, the content and metabolic gene expression level of ascorbic acid (AsA), glutathione, and carotenoid were higher in the MTDR treatment than that in others. Furthermore, the activity and mRNA expression level of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were also promoted in the MTDR group. Combined with these results of important secondary metabolites and protective enzymes measured in the seedlings in different treatments, it could be concluded that exogenous melatonin induced the ascorbic acid-glutathione (AsA-GSH) cycle, carotenoid biosynthesis, and protective enzyme system to improve seedling growth. Our results contribute to the development of a practical method for kiwifruit against drought stress.

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Different gene expression profiles of AD293 and HEK293 cell lines that show contrasting susceptibility to apoptosis induced by overexpression of Bim L.

Acta Biochimica Polonica ◽

10.18388/abp.2006_3323 ◽

2006 ◽

Vol 53 (3) ◽

pp. 525-530 ◽

Cited By ~ 5

Author(s):

Jiayi Zhang ◽

Jinzhong Chen ◽

Lingfeng Liu ◽

Chaoneng Ji ◽

Shaohua Gu ◽

...

Keyword(s):

Gene Expression ◽

Cell Line ◽

Cell Lines ◽

Expression Profile ◽

Hek293 Cell ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Subtractive Hybridization ◽

Hek293 Cells ◽

Differential Expression Profile

Bim is a pro-apoptotic member of the Bcl-2 protein family. Overexpression of Bim proved to be highly cytotoxic for diverse cells. The AD293 cell line is derived directly from the HEK293 cell line but has been transfected with a gene that can improve cell adherence. We found that there was almost no apoptosis seen in Bim L-transfected AD293 cells, but more than half of Bim L-transfected HEK293 cells underwent apoptosis. Suppression subtractive hybridization was used to detect the different gene expression profile between these two cell lines. In 192 sequenced positive clones, there were 30 clones repeating twice or more. Ten genes were selected for identification by semi-quantitative RT-PCR. The transcripts of two adhesion-related genes (actin and parvin) and two apoptosis-related genes (cyclin 2 and protein phosphatase 1G) were up-regulated in AD293 cells. These results suggest that the high expression of cell adhesion-related proteins might be responsible for the different apoptosis status after the transfection of Bim L. Our data provide candidate genes responsible for the different apoptosis sensitivity of these two cell lines. Further investigation on the differential expression profile between AD293 and HEK293 might improve our understanding of cell apoptosis mechanism.

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Transcriptional modulation of AREB-1 by CRISPRa improves plant physiological performance under severe water deficit

Scientific Reports ◽

10.1038/s41598-020-72464-y ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Bruno Paes de Melo ◽

Isabela Tristan Lourenço-Tessutti ◽

Joaquin Felipe Roca Paixão ◽

Daniel David Noriega ◽

Maria Cristina Mattar Silva ◽

...

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Water Deficit ◽

Soluble Sugar ◽

Physiological Adaptation ◽

Sugar Accumulation ◽

Cellular Mechanisms ◽

Physiological Performance ◽

Severe Water Deficit ◽

Element Binding Protein

Abstract Plants are sessile organisms, which are vulnerable to environmental stresses. As such, plants have developed multiple molecular, physiological, and cellular mechanisms to cope with natural stressors. However, these environmental adversities, including drought, are sources of the main agribusiness problems since they interfere with plant growth and productivity. Particularly under water deprivation conditions, the abscisic acid-responsive element-binding protein AREB1/ABF2 plays an important role in drought stress response and physiological adaptation. In this investigation, we provide substantial confirmation for the role of AREB1/ABF2 in plant survival under severe water deficit using the CRISPR activation (CRISPRa) technique to enhance the AREB1 gene expression. In our strategy, the inactive nuclease dCas9 was fused with an Arabidopsis histone acetyltransferase 1, which improves gene expression by remodeling chromatin. The AREB1 overexpression promotes an improvement in the physiological performance of the transgenic homozygous plants under drought, which was associated with an increase in chlorophyll content, antioxidant enzyme activity, and soluble sugar accumulation, leading to lower reactive oxygen species accumulation. Finally, we found that the CRISPR-mediated up-regulation of AREB1 changes the abundance of several downstream ABA-inducible genes, allowing us to report that CRISPRa dCas9-HAT is a valuable biotechnological tool to improve drought stress tolerance through the positive regulation of AREB1.

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