scholarly journals The Integrated Analysis of Transcriptomics and Metabolomics Unveils the Therapeutical Effect of Asiatic Acid on Alcoholic Hepatitis in Rats

2021 ◽  
Author(s):  
Siyun Chen ◽  
Yushen Huang ◽  
Hongmei Su ◽  
Wuchang Zhu ◽  
Yuanyuan Wei ◽  
...  
Keyword(s):  
Alcoholic Hepatitis ◽  
Comprehensive Analysis ◽  
Integrated Analysis ◽  
Asiatic Acid ◽  
Alcohol Metabolism ◽  
Hepatocyte Apoptosis ◽  
Histological Changes ◽  
Metabolism Pathway ◽  
Cyp2e1 Activity ◽  
Hepatocyte Damage

Abstract The present study was to investigate the therapeutical effects and mechanisms of Asiatic acid from Potentilla Chinensis against alcoholic hepatitis. Rats were intragastrically fed with alcohol for 12 weeks to induce alcoholic hepatitis and then treated with various drugs for further 12 weeks. The results showed that Asiatic acid significantly alleviated liver injury caused by alcohol in rats, as evidenced by the improved histological changes and the lower levels of AST, ALT, and TBIL. Besides, Asiatic acid significantly enhanced the activity of ADH and ALDH, promoting alcohol metabolism. Asiatic acid suppressed CYP2E1 activity and NADP+/NADPH ratio, resulting in low ROS production. Further study revealed that Asiatic acid markedly reduced hepatocyte apoptosis by regulating the expression levels of the caspase and Bcl-2 families. Moreover, Asiatic acid could regulate the Keap1/Nrf2 and NF-κB signaling pathway, attenuating oxidative stress and inflammation as a result. Interestingly, the comprehensive analysis of transcriptomics and metabolomics indicated that Asiatic acid regulated the gene expression of Gpat4 and thereby affected the biosynthesis of the metabolites (1-acyl-Sn-glycerol-3-phosphocholine, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine), regulating the glycerophospholipid metabolism pathway and ultimately ameliorating hepatocyte damage. In conclusion, this study demonstrates that Asiatic acid can ameliorate alcoholic hepatitis by modulating the NF-κB and Keap1/Nrf2 signaling pathways and the glycerophospholipid metabolism pathway, which may be developed as a potential medicine for the treatment of alcoholic hepatitis.

scholarly journals Comprehensive Analysis of Transcriptome and Metabolome Reveals the Flavonoid Metabolic Pathway Is Associated with Fruit Peel Coloration of Melon

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2830
Author(s):  
Aiai Zhang ◽  
Jing Zheng ◽  
Xuemiao Chen ◽  
Xueyin Shi ◽  
Huaisong Wang ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Comprehensive Analysis ◽  
Differentially Expressed ◽  
Integrated Analysis ◽  
Fruit Peel ◽  
External Quality ◽  
Color Formation ◽  
Peel Color ◽  
Dark Green

The peel color is an important external quality of melon fruit. To explore the mechanisms of melon peel color formation, we performed an integrated analysis of transcriptome and metabolome with three different fruit peel samples (grey-green ‘W’, dark-green ‘B’, and yellow ‘H’). A total of 40 differentially expressed flavonoids were identified. Integrated transcriptomic and metabolomic analyses revealed that flavonoid biosynthesis was associated with the fruit peel coloration of melon. Twelve differentially expressed genes regulated flavonoids synthesis. Among them, nine (two 4CL, F3H, three F3′H, IFS, FNS, and FLS) up-regulated genes were involved in the accumulation of flavones, flavanones, flavonols, and isoflavones, and three (2 ANS and UFGT) down-regulated genes were involved in the accumulation of anthocyanins. This study laid a foundation to understand the molecular mechanisms of melon peel coloration by exploring valuable genes and metabolites.

Clinical and biological relevance of hepatocyte apoptosis in alcoholic hepatitis

2001 ◽  
Vol 34 (2) ◽  
pp. 254-260 ◽  
Author(s):  
Marianne Ziol ◽  
Maryline Tepper ◽  
Manuel Lohez ◽  
Gilles Arcangeli ◽  
Nathalie Ganne ◽  
...  
Keyword(s):  
Alcoholic Hepatitis ◽  
Hepatocyte Apoptosis ◽  
Biological Relevance

Comprehensive analysis of Actiniopteris Link and Onychium Kaulf. (Pteridophyta) relationships according to their phylogeography, phylogeny and spore morphology

Phytotaxa ◽  
2020 ◽  
Vol 441 (1) ◽  
pp. 1-18
Author(s):  
ALEXEY V. VAGANOV ◽  
JORDAN S. METZGAR ◽  
TATIANA A. SINITSYNA ◽  
ALEXANDER I. SHMAKOV
Keyword(s):  
Interdisciplinary Approach ◽  
Original Data ◽  
Comprehensive Analysis ◽  
Integrated Analysis ◽  
Character State ◽  
Spore Morphology ◽  
Ancestral Character State ◽  
Herbarium Data ◽  
Morphology And Morphometry ◽  
High Level

This research is the first comprehensive analysis of the intrageneric relationships of Actiniopteris and Onychium based on original data including spore morphology, phylogeny and phylogeography. Actiniopteris and Onychium are members of the large subfamily Pteridoideae of the family Pteridaceae. The Pteridaceae family is considered one of the most taxonomically confusing families due to its representatives high level of polymorphism.         We used an interdisciplinary approach to study the “Onychium clade”: 13 taxa were studied using scanning electron microscopy (SEM) to characterize the morphology and morphometry of spores; 14 taxa were studied from a phylogenetic perspective, including character evolution and ancestral character state reconstructions; 15 taxa of “Onychium clade” were studied using herbarium data (B, P, PE, LE, VLA, TI, KYO, ALTB and TK) according to global botanical and geographical zones.                As a result of this integrated analysis, we established a deep divergence of the taxa O. tenuifrons and O. siliculosum from the main composition of the genus Onychium and the division the genus Actiniopteris into two clades: A. radiata–A. semiflabellata and A. australis–A. dimorpha. We found that representatives of Actiniopteris and Onychium had originated in a common African-Indo-Malesian area. The “Onychium clade” center of diversity is the Indian Region, which is experiencing high levels of human impact, leading to disjunctions among the studied taxa.

scholarly journals Alcoholic Hepatitis: A Review

2019 ◽  
Vol 54 (4) ◽  
pp. 408-416 ◽  
Author(s):  
Nooshin Hosseini ◽  
Julia Shor ◽  
Gyongyi Szabo
Keyword(s):  
Alcohol Use ◽  
Alcoholic Hepatitis ◽  
Hepatitis A ◽  
Cell Activation ◽  
Genetic Factors ◽  
Inflammatory Cell ◽  
Stellate Cell ◽  
Hepatic Inflammation ◽  
Hepatocyte Damage ◽  
Pro Inflammatory Cytokines

Abstract Alcoholic liver disease (ALD) represents a spectrum of injury, ranging from simple steatosis to alcoholic hepatitis to cirrhosis. Regular alcohol use results in fatty changes in the liver which can develop into inflammation, fibrosis and ultimately cirrhosis with continued, excessive drinking. Alcoholic hepatitis (AH) is an acute hepatic inflammation associated with significant morbidity and mortality that can occur in patients with steatosis or underlying cirrhosis. The pathogenesis of ALD is multifactorial and in addition to genetic factors, alcohol-induced hepatocyte damage, reactive oxygen species, gut-derived microbial components result in steatosis and inflammatory cell (macrophage and neutrophil leukocyte) recruitment and activation in the liver. Continued alcohol and pro-inflammatory cytokines induce stellate cell activation and result in progressive fibrosis. Other than cessation of alcohol use, medical therapy of AH is limited to prednisolone in a subset of patients. Given the high mortality of AH and the progressive nature of ALD, there is a major need for new therapeutic intervention for this underserved patient population.

Hepatocyte apoptosis is a pathologic feature of human alcoholic hepatitis

2001 ◽  
Vol 34 (2) ◽  
pp. 248-253 ◽  
Author(s):  
Shiho Natori ◽  
Christian Rust ◽  
Linda M. Stadheim ◽  
Anu Srinivasan ◽  
Lawrence J. Burgart ◽  
...  
Keyword(s):  
Alcoholic Hepatitis ◽  
Hepatocyte Apoptosis ◽  
Pathologic Feature ◽  
Human Alcoholic

Infliximab or placebo in severe alcoholic hepatitis: cytokines and hepatocyte apoptosis changes

2002 ◽  
Vol 36 ◽  
pp. 174
Author(s):  
Laurent Spahr ◽  
Laura Rubbia-Brandt ◽  
Jean-Louis Frossard ◽  
Emile Giostra ◽  
Anne-Laure Rougemont ◽  
...  
Keyword(s):  
Alcoholic Hepatitis ◽  
Hepatocyte Apoptosis ◽  
Severe Alcoholic Hepatitis

scholarly journals GeneTEFlow: A Nextflow-based pipeline for analysing gene and transposable elements expression from RNA-Seq data

2020 ◽  
Author(s):  
Xiaochuan Liu ◽  
Jadwiga R Bienkowska ◽  
Wenyan Zhong
Keyword(s):  
Transposable Elements ◽  
Expression Analysis ◽  
Comprehensive Analysis ◽  
Integrated Analysis ◽  
Computational Pipeline ◽  
Immune Functions ◽  
Rna Seq ◽  
One Stop ◽  
Eukaryotic Genomes

AbstractTransposable elements (TEs) are mobile genetic elements in eukaryotic genomes. Recent research highlights the important role of TEs in the embryogenesis, neurodevelopment, and immune functions. However, there is a lack of a one-stop and easy to use computational pipeline for expression analysis of both genes and locus-specific TEs from RNA-Seq data. Here, we present GeneTEFlow, a fully automated, reproducible and platform-independent workflow, for the comprehensive analysis of gene and locus-specific TEs expression from RNA-Seq data employing Nextflow and Docker technologies. This application will help researchers more easily perform integrated analysis of both gene and TEs expression, leading to a better understanding of roles of gene and TEs regulation in human diseases. GeneTEFlow is freely available at https://github.com/zhongw2/GeneTEFlow.

scholarly journals ALDH3B2 Polymorphism Is Associated with Colorectal Cancer Susceptibility

2020 ◽  
Vol 2020 ◽  
pp. 1-5 ◽  
Author(s):  
Zhi-Gang Gao ◽  
Yong Yang ◽  
Xiao-Feng Han ◽  
Yun-Lei Wang ◽  
Zhen-Jun Wang
Keyword(s):  
Colorectal Cancer ◽  
Genetic Variants ◽  
Protein Function ◽  
Odds Ratio ◽  
Functional Annotation ◽  
Cancer Susceptibility ◽  
Alcohol Metabolism ◽  
Metabolism Pathway ◽  
Metabolic Genes ◽  
Coding Variants

Colorectal cancer (CRC) is the 5th leading cancer in China. Alcohol consumption has been reported to be one of the risk factors of CRC. However, it remains unclear whether genetic variants of alcohol metabolic genes are associated with CRC risk. In this study, we tested the coding variants in the alcohol metabolic genes and the risk of CRC, by using 485 cases and 516 controls. A total of 16 germline coding variants in 10 alcohol metabolic genes were genotyped. We identified that rs3741178 in ALDH3B2 was significantly associated with CRC risk with odds ratio being 2.13 (95% CI: 1.24–3.68, P=0.0064). Further functional annotation suggested that this variant may damage the protein function of ALDH3B2. Our results suggested that ALDH3B2 in the alcohol metabolism pathway contributed to the development of CRC, which may contribute to the prevention of this disease in the future.

scholarly journals An interactive web-based application for Comprehensive Analysis of RNAi-screen Data

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Bhaskar Dutta ◽  
Alaleh Azhir ◽  
Louis-Henri Merino ◽  
Yongjian Guo ◽  
Swetha Revanur ◽  
...  
Keyword(s):  
Comprehensive Analysis ◽  
Rnai Screen ◽  
Integrated Analysis ◽  
Expression Data ◽  
Web Based ◽  
Optimal Thresholds ◽  
Analysis Workflow ◽  
Genome Scale ◽  
Sirna Screens ◽  
Selection Of

Abstract RNAi screens are widely used in functional genomics. Although the screen data can be susceptible to a number of experimental biases, many of these can be corrected by computational analysis. For this purpose, here we have developed a web-based platform for integrated analysis and visualization of RNAi screen data named CARD (for Comprehensive Analysis of RNAi Data; available at https://card.niaid.nih.gov). CARD allows the user to seamlessly carry out sequential steps in a rigorous data analysis workflow, including normalization, off-target analysis, integration of gene expression data, optimal thresholds for hit selection and network/pathway analysis. To evaluate the utility of CARD, we describe analysis of three genome-scale siRNA screens and demonstrate: (i) a significant increase both in selection of subsequently validated hits and in rejection of false positives, (ii) an increased overlap of hits from independent screens of the same biology and (iii) insight to microRNA (miRNA) activity based on siRNA seed enrichment.

scholarly journals INTEGRATED ANALYSIS OF THE GENUS BRYUM HEDW. IN THE BRIOFLORA OF BELARUS

2018 ◽  
Vol 63 (3) ◽  
pp. 350-357
Author(s):  
G. F. Rykovsky ◽  
M. S. Shabeta ◽  
H. I. Aniskevich
Keyword(s):  
Comprehensive Analysis ◽  
Integrated Analysis ◽  
The Republic ◽  
First Time

The article presents a comprehensive analysis of the species Bryum in Belarus bryoflora. The genus Bryum has 400–450 species and is represented on the territory of Belarus 31 species, 4 of which are given for the republic for the first time. 

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