scholarly journals Dissecting the Microbial Community Structure of Internal Organs During the Early Postmortem Period in a Murine Corpse Model

2021 ◽  
Author(s):  
Ruina Liu ◽  
Kai Zhang ◽  
Huan Li ◽  
Qinru Sun ◽  
Xin Wei ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
The Body ◽  
Time Since Death ◽  
Limited Information ◽  
Microbial Composition ◽  
Internal Organs ◽  
Organ Specific ◽  
Forensic Microbiology ◽  
The Relationship ◽  
Early Postmortem

Abstract Background Microorganisms inhabit and proliferate throughout the body both externally and internally, which are the primary mediators of putrefaction after death. However, limited information is available about the changes in the postmortem microbiota of extraintestinal body sites in the early decomposition stage of mammalian corpses. Results This study applied 16S rRNA barcoding to investigate microbial composition variations among different organs and the relationship between microbial communities and time since death over 1 day of decomposition. During 1 day of decomposition, Agrobacterium, Prevotella, Bacillus, and Turicibacter were regarded as time-relevant genera in internal organs at different timepoints. Pathways associated with lipid, amino acid, carbohydrate and terpenoid and polyketide metabolism were significantly enriched at 8 hours than that at 0.5 or 4 hours. The microbiome compositions and postmortem metabolic pathways differed by time since death, and more importantly, these alterations were organ specific. Conclusion The dominant microbes differed by organ, while they tended toward similarity as decomposition progressed. The observed thanatomicrobiome variation by body site provides new knowledge into decomposition ecology and forensic microbiology. Additionally, the microbes detected at 0.5 hours in internal organs may inform a new direction for organ transplantation.

scholarly journals Unusual form of relationship between parasitic nematodes of the genus Philonema (Philonemidae) and salmonids

2020 ◽  
Vol 14 (4) ◽  
pp. 24-33
Author(s):  
V. V. Pospekhov ◽  
V. P. Nikishin
Keyword(s):  
Sockeye Salmon ◽  
Negative Impact ◽  
Thick Layer ◽  
Body Cavity ◽  
The Body ◽  
Parasitic Nematodes ◽  
Dolly Varden ◽  
Internal Organs ◽  
Peculiar Form ◽  
The Relationship

The purpose of the research is studying the relationship between nematodes of the genus Philonema (Philonemidae) and salmonids at the organismic level and justification for their interpretation as a peculiar form of encapsulation.Materials and methods. We studied the sockeye salmon, as well as the freshwater white-spotted char and Dolly Varden trout from lakes Kisi and Chistoye (basin of the Ola River, Taui Bay, Sea of Okhotsk). 7 males for breeding sockeye salmon, 39 freshwater white-spotted chars and 16 Dolly Varden trouts were dissected for infection by Philonema. We used well-known generally accepted techniques in the parasitological studies. The nematodes were preserved in 70 % ethanol and clarified in glycerol.Results and discussion. Phylonema causes pathological processes of various intensity in the body cavity of salmonids. As a rule, this is the adhesion of fish’s internal organs and the formation of a thick layer of connective tissue that surrounds the host's organs from outside and forms a kind of a "cocoon". Our studies combined with literature data allow us to consider the formation of a "cocoon" as a peculiar form of encapsulation and an adaptation that minimizes mutual negative impact of the parasite and the host on each other. Further research should determine how widespread this form of encapsulation is in nature, and it is also necessary to study the mechanism of the "cocoon" formation.

scholarly journals The postmortem microbiome and gene expression in vertebrates

2017 ◽  
Vol 39 (2) ◽  
pp. 14-17
Author(s):  
Peter A. Noble ◽  
Alexander E. Pozhitkov
Keyword(s):  
Gene Expression ◽  
Healthy Adult ◽  
Postmortem Interval ◽  
The Body ◽  
Microbial Cell ◽  
Time Since Death ◽  
Internal Organs ◽  
Significant Potential ◽  
Elapsed Time

In life, most internal organs of a healthy adult vertebrate are essentially ‘microbial-cell-free’ – but in death, microorganisms invade and proliferate these internal organs and genes are expressed by the host presumably in response. We recently investigated how these two interconnected processes change with postmortem time and examined the feasibility of using this information to determine the postmortem interval, i.e. the elapsed-time-since-death. Although more research is needed, our findings suggest one of them has a significant potential to determine the length of time from the person dying until discovery of the body.

scholarly journals A comparative analysis of the relationship between stature and ultrasound dimensions of internal organs in adolescents

2021 ◽  
Vol 12 (2) ◽  
pp. 19-27
Author(s):  
Valerii O. Yerkudov ◽  
Andrey P. Pugovkin ◽  
Aleksej J. Volkov ◽  
Oksana I. Musaeva ◽  
Dmitriy J. Volkov ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Body Length ◽  
Individual Characteristics ◽  
The Body ◽  
Comparative Characteristic ◽  
Z Score ◽  
Internal Organs ◽  
Average Group ◽  
The Individual ◽  
The Relationship

The aim of the study was a comparative characteristic of the size of internal organs according to ultrasonography data in subjects with various deviations in stature, determined using international standardized norms. Materials and methods. The stature was measured in 93 adolescents, aged 13 to 17 years. Based on the measurements, the Z-score of body length was calculated according to the WHO Growth Reference, 2007 and three groups were formed for comparing the sizes of internal organs: average, above average, below average. Ultrasonography data of the internal organs dimensions and thyroid gland was performed using a Toshiba Aplio 500 ultrasound scanner. Results. There were statistically significantly lower values of the liver span and the longitudinal size of the gallbladder in the examined subjects from the below average group compared to the rest of the subjects. The length of the spleen and the total volume of the thyroid gland were statistically significantly different in subjects from all three groups, with the highest values in volunteers from the above average group. A weak direct correlation was shown between the Z-score of body length and liver span, the length of the cauda of the pancreas, and the width of the spleen. An average direct statistical relationship was found between the Z-score of stature and the length of the spleen, as well as the total volume of the thyroid gland. Conclusion. To a greater extent, body length is associated with the size of the parenchymal organs with a pronounced connective tissue frame - the liver and spleen, as well as the thyroid gland due to the relationship of its volume with hormones that regulate growth and development. Clinical substantiation of the relationship between the structure of the body and internal organs opens up the possibility of creating anatomical standards that allow ultrasound morphometric assessment of internal organs, taking into account the individual characteristics of the patients body size.

Amino Acids Regulate Glycolipid Metabolism and Alter Intestinal Microbial Composition

2020 ◽  
Vol 21 (8) ◽  
pp. 761-765 ◽  
Author(s):  
Fei Xie ◽  
Zhengqun Liu ◽  
Ming Liu ◽  
Liang Chen ◽  
Wei Ding ◽  
...  
Keyword(s):  
Amino Acids ◽  
Metabolic Pathways ◽  
Metabolic Diseases ◽  
Short Chain Fatty Acids ◽  
Branched Chain Amino Acids ◽  
The Body ◽  
Microbial Composition ◽  
Ampk Signaling ◽  
Glycolipid Metabolism ◽  
Branched Chain

Amino acids (AAs) and their metabolites regulate key metabolic pathways that are necessary for growth, reproduction, immunity and metabolism of the body. It has been convinced that metabolic diseases are closely related to disorders of glycolipid metabolism. A growing number of studies have shown that AAs are closely related to energy metabolism. This review focuses on the effects of amino acids (arginine, branched-chain amino acids, glutamine) and their metabolites (short chain fatty acids) on glycolipid metabolism by regulating PI3K/AKT/mTOR and AMPK signaling pathways and GPCRs receptors, reducing intestinal Firmicutes/Bacteroidetes ratio associated with obesity.

scholarly journals Heterotaxy: fluctuat nec mergitur

2021 ◽  
Vol 31 (7) ◽  
pp. 1200-1201
Author(s):  
Lucile Houyel ◽  
Anne Moreau de Bellaing ◽  
Damien Bonnet
Keyword(s):  
Situs Inversus Totalis ◽  
The Body ◽  
Abdominal Organ ◽  
Heterotaxy Syndrome ◽  
Internal Organs ◽  
Abdominal Organs ◽  
International Pediatric ◽  
Thoracic And Abdominal ◽  
The Relationship ◽  
Visceral Heterotaxy

AbstractThe International Pediatric and Congenital Cardiac Code (IPCCC) states that visceral heterotaxy is defined as “a congenital malformation in which the internal thoraco-abdominal organs demonstrate abnormal arrangement across the left-right axis of the body. By convention, in congenital cardiology, heterotaxy syndrome does not include patients with complete mirror-imaged arrangement of the internal organs along the left-right axis also known as “total mirror imagery” or “situs inversus totalis”.” [www.ipccc.net]In patients with heterotaxy, it is important to describe both the cardiac relations and the junctional connections of the cardiac segments, with documentation of the arrangement of the atrial appendages, the ventricular topology, the nature of the unions of the segments across the atrioventricular and the ventriculoarterial junctions, the infundibular morphologies, and the relationships of the arterial trunks in space. Particular attention is required for the venoatrial connections, since these are so often abnormal. The relationship and arrangement of the remaining thoraco-abdominal organs, including the lungs, the spleen, the liver, and the intestines, also must be described separately, because, although common patterns of association have been identified, there are frequent exceptions to these common patterns. Therefore, in patients with heterotaxy, it is important to describe each thoracic and abdominal organ independently.

scholarly journals Nesfatin-1: Pleiotropic hormone

2020 ◽  
Vol 76 (01) ◽  
pp. 6329-2020
Author(s):  
GRZEGORZ TYMICKI ◽  
IWONA PUZIO ◽  
MARTA PAWŁOWSKA-OLSZEWSKA
Keyword(s):  
Physiological Role ◽  
Pleiotropic Effect ◽  
The Body ◽  
Anatomical Location ◽  
Internal Organs ◽  
Peripheral Tissues ◽  
The Central Nervous System ◽  
Anorexigenic Peptide ◽  
Near Future ◽  
The Relationship

Nesfatin-1 was first identified in 2006 as an anorexigenic peptide involved in appetite regulation. It is expressed both in the central nervous system and in the peripheral tissues. The anatomical location of neurons expressing nesfatin-1 and its co-occurrence with other neurotransmitters in the CNS indicate that its physiological role involves not only the regulation of food intake, but also neuroendocrine regulation and autonomic control of internal organs and behavioral responses. It affects the functions of the digestive tract and the cardiovascular system and plays an important role in the regulation of water intake, sleep, and reproductive functions. It plays an important role in the mechanism of metabolic control of the body, regulation of energy balance and body temperature, and development of obesity. Nevertheless, both the main source of nesfatin-1 in the circulation and the exact mechanisms of its functioning are not fully understood. Considering the pleiotropic effect of nesfatin-1, the relationship between the degree of body fatness and the risk of various diseases and the increase in the expression of nesfatin-1 in adipocytes with the development of obesity, it can be assumed that in the near future it will be taken into account as an important factor in the development, and perhaps treatment, of various diseases.

Composition and Cariogenic Potential of Dental Plaque Fluid

1994 ◽  
Vol 5 (1) ◽  
pp. 1-25 ◽  
Author(s):  
H.C. Margolis ◽  
E.C. Moreno
Keyword(s):  
Dental Plaque ◽  
Lactic Acid Production ◽  
Degree Of Saturation ◽  
Limited Information ◽  
Microbial Composition ◽  
Chemical Changes ◽  
Carious Lesions ◽  
Calculus Formation ◽  
Chemical Events ◽  
The Relationship

Our understanding of the chemical events that take place at the tooth-plaque interface has improved greatly through studies of the chemical composition and properties of dental plaque fluid. In the absence of fermentable carbohydrate, plaque fluid has been found to be supersaturated with respect to tooth mineral and other calcium phosphate phases, thus exhibiting the potential to support calculus formation and the remineralization of incipient carious lesions. Following the exposure to fermentable carbohydrate, the degree of saturation of plaque fluid decreases rapidly, primarily due to lactic acid production and the lowering of plaque fluid pH. The extent of these chemical changes has been shown to be associated with differences in caries history. Such studies have been facilitated by the recent development of microanalytical techniques. Unfortunately, little is known about the relationship between the observed chemical changes in plaque fluid and the microbial composition of plaque. Limited information is also available on the association of immune factors in plaque fluid with dental disease.

The use of Body Surface Index as a Better Clinical Health indicators Compare to Body Mass Index and Body Surface Area for Clinical Application

2018 ◽  
pp. 131-136
Author(s):  
Shirazu I. ◽  
Theophilus. A. Sackey ◽  
Elvis K. Tiburu ◽  
Mensah Y. B. ◽  
Forson A.
Keyword(s):  
Surface Area ◽  
Body Surface Area ◽  
Clinical Application ◽  
Body Surface ◽  
Body Height ◽  
Health Indicators ◽  
The Body ◽  
Body Parameters ◽  
The Relationship ◽  
Individual Body

The relationship between body height and body weight has been described by using various terms. Notable among them is the body mass index, body surface area, body shape index and body surface index. In clinical setting the first descriptive parameter is the BMI scale, which provides information about whether an individual body weight is proportionate to the body height. Since the development of BMI, two other body parameters have been developed in an attempt to determine the relationship between body height and weight. These are the body surface area (BSA) and body surface index (BSI). Generally, these body parameters are described as clinical health indicators that described how healthy an individual body response to the other internal organs. The aim of the study is to discuss the use of BSI as a better clinical health indicator for preclinical assessment of body-organ/tissue relationship. Hence organ health condition as against other body composition. In addition the study is `also to determine the best body parameter the best predict other parameters for clinical application. The model parameters are presented as; modeled height and weight; modelled BSI and BSA, BSI and BMI and modeled BSA and BMI. The models are presented as clinical application software for comfortable working process and designed as GUI and CAD for use in clinical application.

CONCEPTUALISING RISK CULTURE ON ERM IMPLEMENTATION IN CONSTRUCTION COMPANIES

2020 ◽  
Vol 17 (1) ◽  
pp. 59
Author(s):  
Ching Ching Wong
Keyword(s):  
Risk Management ◽  
Risk Identification ◽  
The Body ◽  
Risk Indicators ◽  
Construction Companies ◽  
Risk Appetite ◽  
Risk Culture ◽  
Risk Treatment ◽  
Managing Risk ◽  
The Relationship

Enterprise Risk Management (ERM) is an effective technique in managing risk within an organization strategically and holistically. Risk culture relates to the general awareness, attitudes and behaviours towards risk management in an organisation. This paper presents a conceptual model that shows the relationship between risk culture and ERM implementation. The dependent variable is ERM implementation, which is measured by the four processes namely risk identification and risk assessment; risk treatment; monitor and consult; communicate and consult. The independent variables under risk culture are risk policy and risk appetite; key risk indicators; accountability; incentives; risk language and internal relationships. This study aims to empirically test the relationship between risk culture and ERM implementation among Malaysian construction public listed companies. Risk culture is expected to have direct effects and significantly influence ERM. This study contributes to enhance the body of knowledge in ERM especially in understanding significant of risk culture that influence its’ implementation from Malaysian perspective.

Structural Mediation Model of Information System and Sustainable Performance of UAE Manufacturing Sector

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Mohamed Ahmed Darwish Abdulla Larii ◽  
◽  
Fatma Ahmed Lari ◽  
Mohamed Ahmed Darwish Abdulla Lari ◽  
◽  
...  
Keyword(s):  
Information System ◽  
Manufacturing Sector ◽  
Organisational Culture ◽  
The Body ◽  
Mediating Effect ◽  
Operational Performance ◽  
Manufacturing Companies ◽  
Mediation Model ◽  
Sustainable Performance ◽  
The Relationship

This study intends to find out the mediating effect of organisational culture on the relationship between information system and sustainable performance of manufacturing sector in UAE. This study used AMOS-SEM software to develop mediation model that linking the mediating relationships between Information System, Organisational Culture and Sustainable operation Performance. Data was collected through questionnaire survey among the operation staff of Abu Dhabi manufacturing companies. A total 250 questionnaires were distributed however 205 were returned and only 200 are valid which indicates a response rate of 80%. The analysis found that TPS has positive but not significant effect to SP; OIS has positive but not significant effect to SP; FMW has a positive and significant effect on SP; SDS has a negative and not significant effect to SP and SP has positive but not significant effect OC. For the path relationship between the four exogenous variables (TPS, OIS, SDS, and FMW) and the mediator variable (OC), the results are TPS has positive and significant effect to OC; OIS has positive but not significant effect to OC; FMW has positive and significant effect to OC and SDS has positive and not significant effect to OC. Collectively, the five exogenous constructs (TPS, OIS, SDS, FMW and OC) explained 89% variation in operational performance and 86% of the variation in organisational culture. However, for a mediator, it was found that OC has no significant mediating effect on the relationship between TPS and SP; OC has no significant mediating effect on the relationship between OIS and SP; OC has no significant mediating effect on the relationship between SDS and SP and OC has no significant mediating effect on the relationship between FMW and SP. it can be concluded that there is a positive relationship between information system dimensions and operational performance. However organizational culture has no contributing any mediating effect to the relationship. These findings have contributed to the body of knowledge and could be shared among the UAE manufacturing practitioners.

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