Pyogenic Liver Abscesses Patients With Diabetes Mellitus Induced by Klebsiella Pneumoniae Are More Prone to Organ Dysfunction

2021 ◽  
Author(s):  
Mingming Xue ◽  
Xiangpeng Zeng ◽  
Feixiang Xu ◽  
Mian Shao ◽  
Yilin Yang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Klebsiella Pneumoniae ◽  
Organ Dysfunction ◽  
Liver Abscess ◽  
High Throughput ◽  
Inflammatory Markers ◽  
High Throughput Sequencing ◽  
Glycated Albumin ◽  
Density Lipoprotein ◽  
Significant Difference

Abstract Background Diabetes mellitus (DM) has become a common risk factor for pyogenic liver abscess (PLA) patients, Klebsiella pneumoniae is a primary pathogen of PLA (KPLA). The purpose of this study was to evaluate the clinical and microbiological characteristics in PLA patients with or without DM induced by Klebsiella pneumoniae or not.Methods The clinical data of the total 557 PLA patients were collected in Zhongshan Hospital from January 2015 to December 2020. The liver abscess were confirmed using abdominal ultrasound (US)，computerized tomography (CT), and/or magnetic resonance imaging (MRI). The 557 patients were divided into two groups, PLA with DM and PLA without DM. In the group of PLA with DM, the patients were further separated into KPLA with DM and non-KPLA with DM.Results The total of 557 patients with PLA were analyzed, 225 (40.40%) patients comorbided DM. Among PLA patients with DM, there is a higher proportion of patients with hypertension (42.22%) and fatty liver diseases (38.67%), the most common clinical manifestation is frail and fatigue (18.22%), and more likely to progress to sepsis and metastatic infections such as periorbital infection. The PLA patients with DM have higher inflammatory markers (WBC, N%, CRP, ESR and PCT). There was significant difference between the group of PLA with DM and PLA without DM in the percentage of neutrophils, liver enzymes, albumin, glucose metabolism (blood glucose, glycated hemoglobin and glycated albumin), lipid metabolism (triglycerides, low density lipoprotein, high density lipoprotein), blood sodium and chlorine, blood urea nitrogen and higher heart markers ( proBNP and CK-MB). In KPLA patients with DM compared with non-KPLA with DM, inflammatory markers (WBC, N%, CRP, ESR and PCT), liver function index (TB, CB, ALT, AST, ALP and r-GT) and cardiac markers (cTnT and proBNP) are more higher. The level of albumin, serum sodium and ferritin are more lower between the KPLA patients with DM and non-KPLA with DM. Detection of liver abscess by high-throughput sequencing is more sensitive and accurate. There was no in-hospital mortality.Conclusions The study found that in the PLA patients with diabetic DM, Klebsiella pneumoniae infection is the most common, which is more prone to organ dysfunction and electrolyte disorder. High-throughput sequencing can help early diagnosis and accurate treatment of PLA patients.

scholarly journals Comparison of invertebrate diversity in lake waters and their resting eggs in sediments, as revealed by high-throughput sequencing (HTS)

2020 ◽  
pp. 19
Author(s):  
Xiaoyan Wang ◽  
Qing Wang ◽  
Yufeng Yang ◽  
Wenbo Yu
Keyword(s):  
High Throughput ◽  
Water Samples ◽  
High Throughput Sequencing ◽  
Essential Feature ◽  
Resting Eggs ◽  
Freshwater Ecosystems ◽  
Rrna Gene ◽  
Promising Alternative ◽  
Invertebrate Diversity ◽  
Significant Difference

Aquatic invertebrate diversity reflects water quality and the health of aquatic ecosystems and should be monitored as an essential feature of freshwater ecosystems. The resting eggs of aquatic invertebrates in sediments populate the overlying water. The diversity of invertebrates in waters and their resting eggs in sediments in Baiyangdian Lake, Xiongan, North China, were assessed using high-throughput sequencing (HTS) with a pair of 18S rRNA gene adaptor-linked primers. The total of 99 operational taxonomic units (OTUs) derived from 353,755 invertebrate sequences (mostly zooplankton) were revealed by this study. A total of 50 species in the water samples including 20 rotifers, 11 copepods, 1 cladoceran and 18 other species were sorted out. In the sediment 37 species, including 21 rotifers, 3 copepods, 1 cladoceran and 12 other species, were identified. There were 24 species in common between water and corresponding sediments. Invertebrate OTU richness in water samples was higher than that in sediments (p < 0.01), while there was no significant difference in the Shannon-Wiener index. These results suggest that HTS is a promising alternative for efficient biodiversity assessment and monitoring.

Estimation of Low-Density Lipoprotein Immunoprecipitates in Uniform Drops of Serum

1962 ◽  
Vol 8 (6) ◽  
pp. 616-625 ◽  
Author(s):  
R L Searcy ◽  
T M Asher ◽  
L M Bergquist
Keyword(s):  
Diabetes Mellitus ◽  
Coronary Artery Disease ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Healthy Population ◽  
Low Density ◽  
Significant Difference ◽  
Microcapillary Columns ◽  
Artery Disease ◽  
Ultracentrifugal Analysis

Abstract An immunochemical technic has been found to be specific, efficient and reproducible in removing lipoproteins of density less than 1.063 gm./ml. from measured drops of human serum. Estimations of microcapillary columns of immunoprecipitate compare favorably with ultracentrifugal analysis of Sf 0-400 lipoproteins. An equation derived from these data converts millimeters of lipoprotein precipitate into milligrams lipoprotein Sf 0-400 per 100 ml. serum. A highly significant difference was obtained between mean lipoprotein precipitate levels of a clinically healthy population and those of a group of patients with coronary artery disease or diabetes mellitus.

scholarly journals Glycated albumin and direct low density lipoprotein cholesterol levels in type 2 diabetes mellitus

2009 ◽  
Vol 406 (1-2) ◽  
pp. 71-74 ◽  
Author(s):  
Masumi Ai ◽  
Seiko Otokozawa ◽  
Ernst J. Schaefer ◽  
Bela F. Asztalos ◽  
Katsuyuki Nakajima ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Glycated Albumin ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Cholesterol Levels

scholarly journals A Rare Case of Recurrent Klebsiella pneumoniae Liver Abscess

2021 ◽  
Vol 2021 ◽  
pp. 1-4
Author(s):  
Magdelene Amoateng ◽  
Pius Osei-Bagyina ◽  
Reba Varughese ◽  
Achsah Mathew ◽  
Ishan Malhotra
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factor ◽  
Klebsiella Pneumoniae ◽  
Liver Abscess ◽  
High Mortality ◽  
Rare Case ◽  
Pyogenic Liver Abscess ◽  
Important Risk Factor ◽  
Aggressive Treatment

Klebsiella pneumoniae liver abscess (KPLA) is an emerging syndrome with the initial cases described in Taiwan in the 1980s. There is high mortality with this condition, and immediate aggressive treatment is necessary. Diabetes mellitus (D.M.) is the single most important risk factor for developing KPLA. Here, we describe a rare case of recurrent cryptogenic Klebsiella pneumoniae pyogenic liver abscess (KPLA) in a young man with poorly controlled type 1 D.M.

scholarly journals Prospective Evaluation of Coexistence of Subclinical Hypothyroidism with Type 2 Diabetes Mellitus and Its Effect on Metabolic Profile

2021 ◽  
Vol 8 (10) ◽  
pp. 547-550
Author(s):  
Sri Krishna Appaji C ◽  
Deepak Phanindhra M ◽  
Anand Acharya
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Subclinical Hypothyroidism ◽  
Metabolic Profile ◽  
Density Lipoprotein ◽  
Thyroid Stimulating Hormone ◽  
Serum Free ◽  
Significant Difference

BACKGROUND Subclinical hypothyroidism is the term used to describe a condition where there is isolated elevated serum thyroid stimulating hormone level in the setting of normal serum free thyroxin levels in the absence or presence of symptoms. There is no uniformity in various studies regarding association between subclinical hypothyroidism and type 2 diabetes mellitus with regard to prevalence and change in metabolic profile. METHODS After enrolment of patients, a detailed relevant history of patients was taken and detailed clinical examination was done. All information obtained from patients was recorded in predesigned proforma. After 8 hours of fasting, serum levels of glucose, HbA1c, total cholesterol, high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) by fully automated clinical biochemistry analyser EM 200 were checked. Serum free T3, free T4 and thyroid stimulating hormone (TSH) were measured using standard assay. RESULTS Fasting glucose was significantly higher (P = .00001) in diabetes mellitus plus subclinical hypothyroidism group than patients with diabetes mellitus (DM) and euthyroid group (156.3 + 15.77 mg / dl vs. 128.1 + 21.44 mg / dl). Fasting insulin was significantly higher (P = .00001) in diabetes mellitus plus subclinical hypothyroidism group than patients with DM and euthyroid group (3.69 + 0.82 mu / L vs. 2.36 + 0.75 mu / L). There was no significant difference between two groups regarding HbA1c. CONCLUSIONS From our study we conclude that prevalence of subclinical hypothyroidism was higher in type 2 diabetes patients than normal population and there was female predominance. Body mass index was significantly higher in subclinical hypothyroidism group and there was no significant difference between the two groups regarding duration of diabetes mellitus. Patients with subclinical hypothyroidism have poor glycaemic control. KEYWORDS Subclinical Hypothyroidism, Diabetes Mellitus, Metabolic Profile

scholarly journals Gender based variation of lipid abnormalities in type-2 diabetes mellitus patients

2021 ◽  
Vol 9 (10) ◽  
pp. 3068
Author(s):  
Arpita Jaidev ◽  
Hitesh Shah ◽  
Liggy Andrews ◽  
Bhavisha N. Vagheda
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Total Cholesterol ◽  
Blood Sugar ◽  
Density Lipoprotein ◽  
Diabetic Patients ◽  
Male And Female ◽  
Significant Difference

Background: Dyslipidemia has a varying pattern among the male and female patients of type 2 diabetes mellitus (DM).Methods: This study was conducted in the out-patient department (OPD) of department of medicine at GMERS, Patan, Gujarat from July 2020 to December 2020 for a period of six months. Fasting blood sugar, hemoglobin A1c (FBS, HbA1c) lipid profile triacylglycerol-triglyceride, total cholesterol, low density lipoprotein cholesterol and high-density lipoprotein cholesterol (TG, TC, LDL-C, and HDLC) were measured. Statistical analyses were performed with the SPSS software program.Results: A total number of 200 type 2 DM patients (100 males and 100 females) attending to GMERS OPD were recruited in this study. Blood sugar was higher than normal in both male and female (FBS=142.44±36.21, 146.40±41.49 respectively). TG level was also higher in two groups of study subjects with female level slightly more than male (164.99±67.1and 138.21±70 respectively) with no significant difference between the groups (p>0.05).Total cholesterol and LDL-C level was within normal physiological level in both groups, where-as these levels were higher in female in comparison to male (TC=198.07±40.82 and 169.5±36.13 respectively, LDLC=118±34 and 99±27, respectively), showing significant difference between the groups (p=0.014). HDL-C was not below normal in both male (41±5.4) and female (43.99±4.31); however, HDL-C was slightly higher in female than male and the difference was significant (p=0.0129).Conclusions: Dyslipidemia was noticed in a greater proportion of female diabetic patients than male diabetic patients.

scholarly journals The THE ROLE OF ASTAXANTHIN COMPARED WITH METFORMIN IN PREVENTING GLYCATED HUMAN SERUM ALBUMIN FROM POSSIBLE UNFOLDING: A MOLECULAR DYNAMIC STUDY

2019 ◽  
pp. 276-282
Author(s):  
SYAHPUTRA WIBOWO ◽  
SRI WIDYARTI ◽  
AKHMAD SABARUDIN ◽  
DJOKO WAHONO SOEATMADJI ◽  
SUTIMAN BAMBANG SUMITRO
Keyword(s):  
Diabetes Mellitus ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Root Mean Square ◽  
Conformational Changes ◽  
Glycated Albumin ◽  
Mean Square ◽  
Discovery Studio ◽  
Significant Difference

Objectives: Albumin in diabetes mellitus undergoes conformational changes that affect the ability as an endogenous scavenger. Treatment with astaxanthin (ASX) expected to improve the function of albumin in case of diabetes mellitus. The objectives of this study are to compare the capability of ASX and metformin to prevent conformational changes on glycated albumin. Methods: Data mining is performed to obtain human serum albumin (HSA) (4K2C), glucose (79025), ASX (5281224), and metformin (4091). Data preparation used PyRx and Discovery Studio 2016 Client. PyRx is utilized for docking and analysis of receptor-ligand interactions with LigPlus and Discovery Studio 2016 Client. YASARA is used for molecular dynamics simulations with a running time of 15.000 ps. Results: A description of the glycated-HSA (gHSA) conformational changes that are bound to metformin has been successfully carried out. Changes that occur were unfolding and release of bonds in gHSA. Unfolding on gHSA includes the release of bonds between sites A and B. The root mean square deviation (RMSD) backbone value of metformin-gHSA shows a significant difference with gHSA at 8650 ps where gHSA showed 6.47 nm while the metformin-gHSA was 8.06 nm and continues to increase up to 15.72 nm at the end of the simulation. RMSD and root mean square fluctuation residues of gHSA which were interacted with ASX showed conditions close to normal HSA. In 11725 ps ASX-gHSA remained stable at 5.78 nm, whereas gHSA increased to 8.13 nm. gHSA at the end of the simulation showed a number of 9.052 nm while the normal HSA was 7.561 nm. Conclusion: This result indicated that ASX prevents gHSA from possible unfolding.

scholarly journals Comparison of the Intestinal Microbial Community in Ducks Reared Differently through High-Throughput Sequencing

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Yan Zhao ◽  
Kun Li ◽  
Houqiang Luo ◽  
Longchuan Duan ◽  
Caixia Wei ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Water Contamination ◽  
High Throughput Sequencing ◽  
Free Access ◽  
Operational Taxonomic Units ◽  
Genus Level ◽  
Significant Difference ◽  
Access To Water ◽  
Poultry Droppings

Birds are an important source of fecal contamination in environment. Many of diseases are spread through water contamination caused by poultry droppings. A study was conducted to compare the intestinal microbial structure of Shaoxing ducks with and without water. Thirty 1-day-old Shaoxing ducks (Qingke No. 3) were randomly divided into two groups; one group had free access to water (CC), while the other one was restricted from water (CT). After 8 months of breeding, caecal samples of 10 birds from each group were obtained on ice for high-throughput sequencing. A total of 1507978 valid sequences were examined and clustered into 1815 operational taxonomic units (OTUs). At phylum level, Firmicutes (41.37%), Bacteroidetes (33.26%), Proteobacteria (13.67%), and Actinobacteria (8.26%) were found to dominate the microbial community in CC birds, while Firmicutes (53.62%), Bacteroidetes (33.06%), and Actinobacteria (11.13%) were uncovered to be the prime phyla in CT ducks. At genus level, Bacteroides (25.02%), Escherichia-Shigella (11.02%), Peptococcus (7.73%) and Parabacteroides (5.86%) were revealed to be the mainly genera in the CC group ducks, while Bacteroides (18.11%), Erysipelatoclostridium (10.94%), Ruminococcaceae_unclassified (10.43%), Lachnospiraceae_unclassified (5.26%), Coriobacteriales_unclassified (5.89%), and Faecalibacterium (4.2%) were detected to staple the microbial flora in the CT birds. One phylum and 13 genera were found to have the significant difference between the two bird groups (p<0.05). At phylum level, Proteobacteria in CT ducks were found to be obviously lower than ducks in CC birds (p<0.05). At genus level, Escherichia-Shigella (p<0.05) and Peptococcus (p<0.05) were found to be notably lower in CT birds, while Erysipelatoclostridium (p<0.05), Ruminococcaceae_unclassified (p<0.01), Coriobacteriales_unclassified (p<0.05), Faecalibacterium (p<0.01), Atopobiaceae_unclassified (p<0.01), Alistipes (p<0.05), Eggerthellaceae_unclassified (p<0.05), Prevotella_7 (<0.05), Rikenellaceae_RC9_gut_group (p<0.05), Prevotellaceae_uncultured (p<0.05), and Shuttleworthia (p<0.05) were observed to be prominently higher in CT ducks. In conclusion, the present study revealed the effects of keeping ducks away from swimming with obvious changes in the microbial community. Though higher microbial richness was found in the ducks without swimming, more pathogenic genera including Eggerthella, Erysipelatoclostridium, Alistipes, Prevotella_7, and Shuttleworthia; zoonotic genera including Eggerthella and Shuttleworthia; inflammatory genus Alistipes; anti-inflammatory Faecalibacterium genus; and tumor genus Rikenellaceae were examined in these ducks. The CT ducks also showed significant changes at genera level regarding the metabolism (Peptococcus, Ruminococcaceae, and Coriobacteriales).

scholarly journals Thymoquinone, the Most Prominent Constituent of Nigella Sativa, Attenuates Liver Damage in Streptozotocin-Induced Diabetic Rats via Regulation of Oxidative Stress, Inflammation and Cyclooxygenase-2 Protein Expression

2021 ◽  
Vol 11 (7) ◽  
pp. 3223
Author(s):  
Saleh A. Almatroodi ◽  
Abdullah M. Alnuqaydan ◽  
Mohammed A. Alsahli ◽  
Amjad Ali Khan ◽  
Arshad Husain Rahmani
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Antioxidant Enzymes ◽  
Blood Glucose ◽  
Liver Function ◽  
Protein Expression ◽  
Inflammatory Markers ◽  
Density Lipoprotein ◽  
Diabetic Rats

Diabetes mellitus (DM) is a multifaceted metabolic disorder that results in dysfunction and failure of various organs. The present study aimed to evaluate the role of Thymoquinone (TQ), on antidiabetic, oxidative stress, and anti-inflammatory activities in streptozotocin (STZ)-induced (55 mg/kg b.w) diabetic rats. TQ was orally given for 8 consecutive weeks at dose of 150 mg/kg b.w. The blood glucose, insulin, total cholesterol, triglycerides, liver function enzymes, high density lipoprotein (HDL)-cholesterol, and low-density lipoprotein (LDL)-cholesterol levels were measured accordingly in control, diabetes control (DC), and TQ-treatment groups. These experiments confirmed that TQ conserves the insulin level (0.4 ng/mL vs. 0.23 ng/mL), fasting blood glucose (146 ± 7 mg/dL vs. 225 ± 5 mg/dL), and HbA1c (7.5% vs. 10.6%) quite considerably as compared to DC animals. Our results also confirmed that TQ treatment conserves the body weight and lipid profile significantly in STZ-treated animals as compared to the DC group. Moreover, the antioxidant enzymes (GSH, SOD, GST, and CAT) levels decreased, liver function enzymes (ALT, AST, and ALP), lipid peroxidation and inflammatory markers (TNF-α, CRP, IL-1β, IL-6) increased by STZ treatment, that is significantly restored after TQ treatment. As compared to untreated animals, TQ restored the hepatocytes architectural changes and collagen fibers and cox-2 protein expression in liver tissues as evaluated by hematoxylin and eosin, Masson’s trichrome, and immunohistochemistry staining. Taken together, all these findings indicated that TQ ameliorates glucose level and lipid metabolism. It restores liver function, antioxidant enzymes, anti-inflammatory markers, and maintains hepatocytes architecture in STZ-induced diabetes mellitus rats. Here, in this study, we have demonstrated for the first time the role of TQ in the reduction of the expression of cyclooxygenase-2 and fibrosis formation in diabetic rats. Based on the findings, the study suggests that TQ is a novel natural drug with a wide range of clinical applications including the management of diabetes mellitus.

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