The Transcription Factor HcERF4 Confers Salt and Drought Tolerance in Kenaf (Hibiscus Cannabinus L.)

2021 ◽  
Author(s):  
Jiao Yue ◽  
Meiqiong Tang ◽  
Hui Zhang ◽  
Dengjie Luo ◽  
Shan Cao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Hibiscus Cannabinus ◽  
Amino Acid Residues ◽  
Response Factors ◽  
Relative Water ◽  
Localization Analysis ◽  
Drought Stress Condition ◽  
Ethylene Response Factors ◽  
Erf Transcription Factor

Abstract Ethylene response factors (ERF) are members of the APETALA2/ERF transcription factor family, and they play an important role in plant growth, development, and response to various environmental stresses. In the present study, an ERF transcription factor HcERF4 was isolated and characterized from kenaf. The protein encoded by the HcERF4 has 233 amino acid residues with a theoretical isoelectric point of 8.89 and a predicted molecular weight of 25.53 kDa. HcERF4 had an over than 86.97% identity to HsERF4(XP_039019980.1), and shared a closest phylogenetic relationship with Hibiscus syriacus. Subcellular localization analysis shows that HcERF4 is located in the nucleus. Transactivation assays in yeast demonstrated that HcERF4 functions as a transcriptional activator. The expression of HcERF4 was enriched in leaf and root, and can be induced by salt or drought treatments in kenaf. The VIGS-silenced HcERF4 plant showed significantly reduced plant height, stem diameter, fresh weight, and relative water content (RWC) compared with wild type plants under salt or drought stress condition; In addition, the contents of MDA, O2−, H2O2, and free proline is significantly increased, and the activities of SOD and CAT are significantly reduced. The DAB/NBT staining results showed that the H2O2 and O2− contents in HcERF4-silenced plants were consistent with the determination. Based on these results, it is proposed that HcERF4 plays an important role in regulating salt and drought stress in kenaf.

scholarly journals Screening of Mungbean Genotypes under Polyethylene Glycol (PEG) Induced Drought Stress Condition

2020 ◽  
pp. 1-12
Author(s):  
Abdullah All Imtiaz ◽  
Saleh Ahmed Shahriar ◽  
Md. Abdullahil Baque ◽  
Most. Nurjahan Khatun Eaty ◽  
Maliha Rahman Falguni
Keyword(s):  
Drought Stress ◽  
Water Retention ◽  
Stress Condition ◽  
Dry Weight ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
Shoot Dry Weight ◽  
Root Shoot Ratio ◽  
Relative Water ◽  
Drought Stress Condition

Sixteen advance genotypes of mungbeans under 5 different concentrations of Polyethylene Glycol (PEG) were studied to find out the better cultivar against drought stress condition. The experiment results revealed that germination, seedling production and water-related behavior of mungbean genotypes differed significantly under different PEG (drought inducer) concentrations. The mungbean genotype BINA Mung-6 (V8) is proved as highly tolerant against drought stress condition among all other tested genotypes. The results of the investigation revealed that BINA Mung-6 (V8) genotype consistently scored the highest value for all parameters except for the root shoot ratio and water retention capacity that was statistically comparable to genotypes BARI Mung-4 (V2) and BINA Mung-5 (V7). Consistently poor performance were recorded from IPM-02-03 (V16) genotype which is statistically similar as genotypes BMXK1-09015-2 (V13) and BMXK1-09015-6 (V10). The maximum percentage of germination (98.12%), shoot length (139.40 mm), root length (99.07 mm), shoot dry weight (22.32 mg), root dry weight (6.88 mg), relative water content (94.78), water retention capacity (24.98), germination co-efficient (22.27) and vigor index (233.90) were reported from BINA Mung-6 (V8) at a concentration of 0 percent PEG. The minimum percentage of germination (28.22 percent), shoot length (31.17 mm), root length (16.50 mm), shoot dry weight (2.21 mg), root dry weight (0.97 mg), relative water content (25.55), water retention capacity (3.08), germination co-efficient (6.06) and vigor index (13.45) were reported from IPM-02-03 (V16) mungbean advance lines at 0 percent PEG. Maximum (0.92) root shoot ratio was recorded from both BARI Mung-8 (V6) and BMX-08011-2 (V11) mungbean genotypes at 20 percent PEG concentration and minimum (0.22) at 0 percent PEG concentration from BARI Mung-5 (V3) genotype. Maximum water retention capacity (74.45) was recorded at 20 per cent PEG concentration from IPM-02-03 (V16) genotype and minimum (5.22) was at 0 per cent PEG concentration from BINA Mung-6 (V8) genotype.

scholarly journals Impact of Drought Stress on the Accumulation of Capsaicinoids in Capsicum Cultivars with Different Initial Capsaicinoid Levels

HortScience ◽  
2012 ◽  
Vol 47 (9) ◽  
pp. 1204-1209 ◽  
Author(s):  
Paongpetch Phimchan ◽  
Suchila Techawongstien ◽  
Saksit Chanthai ◽  
Paul W. Bosland
Keyword(s):  
Drought Stress ◽  
Water Potential ◽  
Leaf Water Potential ◽  
Leaf Water ◽  
Growth And Yield ◽  
Yield Response ◽  
Hot Pepper ◽  
Environment Interaction ◽  
Relative Water ◽  
Drought Stress Condition

Capsaicinoids are the alkaloids in hot pepper that cause the sensation of heat when eaten and are affected by a genetic and environment interaction. Drought stress is well recognized as an environmental condition that influences capsaicinoid accumulation. This investigation identified the responses of capsaicinoid accumulation in hot pepper cultivars under drought stress condition. A total of nine cultivars with a different initial pungency level, i.e., low, medium, and high, was subjected to gradual drought stress during the flowering stage. Plants in this drought stress group were supplied with reduced water applications of 25%, 50%, and 75% by volume at 10, 20, and 30 days after flowering (DAF), respectively. Leaf water potential and relative water content were recorded to measure the level of drought stress. The results indicated that all cultivars were subjected to drought stress because of their decrease in leaf water potential and changes in physiological characteristics, e.g., growth and yield performance. In addition, leaf area and shoot-to-root ratio were good criteria for identifying hot pepper cultivars under drought stress because their responses were correlated with the stress level and yield components. Yield performances of the high pungency group did not decrease under drought stress, whereas those of the low pungency group did decrease. In conclusion, capsaicinoid levels increased for all cultivars studied when subjected to drought stress, except for the cultivars in the high pungency group. A yield response under drought stress for the medium pungency group varied and was not found to be associated with drought stress.

scholarly journals The ERF transcription factor MdERF38 promotes drought stress‐induced anthocyanin biosynthesis in apple

2019 ◽  
Vol 101 (3) ◽  
pp. 573-589 ◽  
Author(s):  
Jian‐Ping An ◽  
Xiao‐Wei Zhang ◽  
Si‐Qi Bi ◽  
Chun‐Xiang You ◽  
Xiao‐Fei Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Anthocyanin Biosynthesis ◽  
Erf Transcription Factor

scholarly journals Improvement of drought tolerance in white clover (Trifolium repens) by transgenic expression of a transcription factor gene WXP1

2010 ◽  
Vol 37 (2) ◽  
pp. 157 ◽  
Author(s):  
Qingzhen Jiang ◽  
Ji-Yi Zhang ◽  
Xiulin Guo ◽  
Mohamed Bedair ◽  
Lloyd Sumner ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Water Potential ◽  
Water Content ◽  
White Clover ◽  
Trifolium Repens ◽  
Relative Water Content ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Relative Water

White clover (Trifolium repens L.) is an important pasture legume in many regions of the world. A commercial cultivar of white clover (cv. Patriot) was transformed with a Medicago truncatula L. transcription factor gene, WXP1, and a reporter gene, β-glucuronidase (GUS). The WXP1 gene and the GUS gene were placed under control of the Arabidopsis CER6 promoter. GUS staining and cross-section analysis revealed the CER6 promoter directed constitutive expression in leaves and epidermis preferential expression in petioles of white clover. Independent transgenic WXP1 lines, empty vector and wild-type controls were subjected to drought stress treatment. The plants were characterised by measuring several physiological parameters including gas exchange, chlorophyll fluorescence, relative water content and leaf water potential. The WXP1 transgenic lines had higher net photosynthetic rates, higher efficiency of PSII, higher relative water content and leaf water potential under drought-stressed conditions. Consistent with the results from physiological analyses, the transgenic white clover plants carrying WXP1 showed improved tolerance to drought stress.

scholarly journals Indole -3-acetic acid improves drought tolerance of white clover associated with activating auxin-related genes, abscisic acid and jasmonic acid-induced stress responsive transcription factors, and inhibiting senescence genes

2020 ◽  
Author(s):  
Youzhi Zhang ◽  
Yaping Li ◽  
Muhammad Jawad Hassan ◽  
Zhou Li ◽  
Yan Peng
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Drought Resistance ◽  
White Clover ◽  
Dry Weight ◽  
Expression Levels ◽  
Regulated Expression ◽  
Transcription Factor Genes ◽  
Relative Water ◽  
Exogenous Iaa

Abstract Auxin plays an important role in regulating plant development. To idenfity what effects IAA has in white clover under drought stress and how IAA regulates white clover response to drought. Relative water content, chlorophyll content, six phytohormones content, expression of gene-related to IAA signal perception and synthesis, transcription factor genes related to drought-resistance and senescence genes were investigated in white clover pretreated with 1µM exogenous IAA or 100µM L-AOPP (inhibitor of IAA synthesis) under 15% polyethylene glycol-6000 (PEG-6000). Compared to control, drought stress significantly diminished stem dry weight (g/10 plant), relative water content (RWC, %) and total chlorophyll content (Chl, mg/g). Exogenous IAA significantly increased RWC and Chl, however, L-AOPP decreased stem dry weight, RWC and Chl compared with direct drought treatment. Besides that, compared with direct drought set, exogenous IAA significantly improved ABA content and JA content, up-regulated expression levels of some auxin-responsive genes ( GH3.1 , GH3.9 , IAA8 ) , transcription factor genes ( bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 ) related to drought-resistance and drought resistance gene ( RD22 ). Meanwhile, exogenous IAA down-regulated expression levels of several auxin-responsive genes ( GH3.3 , GH3.6 , IAA27 ) and leaf senescence genes ( SAG101 and SAG102 ). Contrarily, L-AOPP significantly reduced ABA, GA3 and JA content, down-regulated expression levels of GH3.1 , GH3.9 , IAA8 , bZIP11 , DREB2 , MYB14 , MYB48 , WRKY2 , WRKY56 , WRKY108715 , ERD and RD22 , and up-regulated expression levels of SAG101 and SAG102 . Collectively, these data suggested a positive role of exogenous IAA in alleviating drought damage through many involved physiological processes in white clover.

scholarly journals In vitro screening for drought tolerance of some chickpea varieties in Bangladesh

2016 ◽  
Vol 27 (2) ◽  
pp. 110-118 ◽  
Author(s):  
UK Salma ◽  
F Khatun ◽  
MJH Bhuiyan ◽  
S Yasmin ◽  
TH Khan
Keyword(s):  
Drought Stress ◽  
Dry Weight ◽  
Germination Percentage ◽  
Proline Content ◽  
In Vitro Screening ◽  
Fresh Weight ◽  
Relative Water ◽  
Drought Stress Condition ◽  
Different Levels

The aim of the present study was to study physiological response of seven chickpea varieties viz. Binachola-2, Binachola-3, Binachola-4, Binachola-5, Binachola-6, Binachola-7 and Binachola-8 at different levels of drought stress induced by polyethylene glycol (PEG).  Five different concentrations (0, 20, 35, 50, 60 g/L) of PEG 6000 was added in MS medium to create five different levels of drought stress for  in vitro screening of drought tolerant chick pea varieties. Data were recorded on germination percentage, fresh weight, shoot length, root length, dry weight, turgid weight, relative water content (RWC) and proline content. The seven chickpea varieties differed significantly for different parameters in response to the drought stress. Binachola-2 and Binachola-7 showed the best performance for all the parameters studied. At the highest dose of PEG (60 g/l), in the most water deficient condition, fresh weight was recorded 0.59 g and 0.84 g, Shoot lengths of 2.10 cm and 3.75 cm, root lengths of 1.15 cm and 1.00 cm, turgid weight of 0.960 g and 0.970 g, dry weight of 0.13 g and 0.21 g, relative water contents (RWC) of 85.71% and 83.33% were recorded in Binachola-2 and Binachola-7, respectively. Increased proline content was recorded with the increasing level of PEG concentration. Proline content 0.533g/100g FW and 0.598g/100g FW were observed in Binachola-2 and Binachola-7, respectively under the influence of PEG at 60 g/l. Data recorded for Binachola-3, Binachola-4, Binachola-5, Binachola-6 and Binachola-8 for the studied parameters revealed that they showed susceptible response against higher drought stress level gen­erated by PEG. The data of this experiment revealed that, the accumulation of proline was significantly greater under drought stress. Thus, it is evident that Binachola-2 and Binachola-7 performed better against drought stress condition. These results of physiological and biochemical parameters may be utilized as a selection indicator for breeding program and used as a baseline for improvement of chickpea varieties in Bangladesh.Progressive Agriculture 27 (2): 110-118, 2016

scholarly journals An ERF Transcription Factor Gene from Malus baccata (L.) Borkh, MbERF11, Affects Cold and Salt Stress Tolerance in Arabidopsis

Forests ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 514 ◽  
Author(s):  
Deguo Han ◽  
Jiaxin Han ◽  
Guohui Yang ◽  
Shuang Wang ◽  
Tianlong Xu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Forest Tree ◽  
Ethylene Response Factor ◽  
Amino Acid Residues ◽  
Response Factors ◽  
Factor Gene ◽  
Ethylene Response ◽  
Mda Content ◽  
Malus Baccata ◽  
Ethylene Response Factors

Apple, as one of the most important economic forest tree species, is widely grown in the world. Abiotic stress, such as low temperature and high salt, affect apple growth and development. Ethylene response factors (ERFs) are widely involved in the responses of plants to biotic and abiotic stresses. In this study, a new ethylene response factor gene was isolated from Malus baccata (L.) Borkh and designated as MbERF11. The MbERF11 gene encoded a protein of 160 amino acid residues with a theoretical isoelectric point of 9.27 and a predicated molecular mass of 17.97 kDa. Subcellular localization showed that MbERF11 was localized to the nucleus. The expression of MbERF11 was enriched in root and stem, and was highly affected by cold, salt, and ethylene treatments in M. baccata seedlings. When MbERF11 was introduced into Arabidopsis thaliana, it greatly increased the cold and salt tolerance in transgenic plant. Increased expression of MbERF11 in transgenic A. thaliana also resulted in higher activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), higher contents of proline and chlorophyll, while malondialdehyde (MDA) content was lower, especially in response to cold and salt stress. Therefore, these results suggest that MbERF11 probably plays an important role in the response to cold and salt stress in Arabidopsis by enhancing the scavenging capability for reactive oxygen species (ROS).

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