scholarly journals In vitro screening for drought tolerance of some chickpea varieties in Bangladesh

2016 ◽  
Vol 27 (2) ◽  
pp. 110-118 ◽  
Author(s):  
UK Salma ◽  
F Khatun ◽  
MJH Bhuiyan ◽  
S Yasmin ◽  
TH Khan
Keyword(s):  
Drought Stress ◽  
Dry Weight ◽  
Germination Percentage ◽  
Proline Content ◽  
In Vitro Screening ◽  
Fresh Weight ◽  
Relative Water ◽  
Drought Stress Condition ◽  
Different Levels

The aim of the present study was to study physiological response of seven chickpea varieties viz. Binachola-2, Binachola-3, Binachola-4, Binachola-5, Binachola-6, Binachola-7 and Binachola-8 at different levels of drought stress induced by polyethylene glycol (PEG).  Five different concentrations (0, 20, 35, 50, 60 g/L) of PEG 6000 was added in MS medium to create five different levels of drought stress for  in vitro screening of drought tolerant chick pea varieties. Data were recorded on germination percentage, fresh weight, shoot length, root length, dry weight, turgid weight, relative water content (RWC) and proline content. The seven chickpea varieties differed significantly for different parameters in response to the drought stress. Binachola-2 and Binachola-7 showed the best performance for all the parameters studied. At the highest dose of PEG (60 g/l), in the most water deficient condition, fresh weight was recorded 0.59 g and 0.84 g, Shoot lengths of 2.10 cm and 3.75 cm, root lengths of 1.15 cm and 1.00 cm, turgid weight of 0.960 g and 0.970 g, dry weight of 0.13 g and 0.21 g, relative water contents (RWC) of 85.71% and 83.33% were recorded in Binachola-2 and Binachola-7, respectively. Increased proline content was recorded with the increasing level of PEG concentration. Proline content 0.533g/100g FW and 0.598g/100g FW were observed in Binachola-2 and Binachola-7, respectively under the influence of PEG at 60 g/l. Data recorded for Binachola-3, Binachola-4, Binachola-5, Binachola-6 and Binachola-8 for the studied parameters revealed that they showed susceptible response against higher drought stress level gen­erated by PEG. The data of this experiment revealed that, the accumulation of proline was significantly greater under drought stress. Thus, it is evident that Binachola-2 and Binachola-7 performed better against drought stress condition. These results of physiological and biochemical parameters may be utilized as a selection indicator for breeding program and used as a baseline for improvement of chickpea varieties in Bangladesh.Progressive Agriculture 27 (2): 110-118, 2016

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

scholarly journals Screening of Mungbean Genotypes under Polyethylene Glycol (PEG) Induced Drought Stress Condition

2020 ◽  
pp. 1-12
Author(s):  
Abdullah All Imtiaz ◽  
Saleh Ahmed Shahriar ◽  
Md. Abdullahil Baque ◽  
Most. Nurjahan Khatun Eaty ◽  
Maliha Rahman Falguni
Keyword(s):  
Drought Stress ◽  
Water Retention ◽  
Stress Condition ◽  
Dry Weight ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
Shoot Dry Weight ◽  
Root Shoot Ratio ◽  
Relative Water ◽  
Drought Stress Condition

Sixteen advance genotypes of mungbeans under 5 different concentrations of Polyethylene Glycol (PEG) were studied to find out the better cultivar against drought stress condition. The experiment results revealed that germination, seedling production and water-related behavior of mungbean genotypes differed significantly under different PEG (drought inducer) concentrations. The mungbean genotype BINA Mung-6 (V8) is proved as highly tolerant against drought stress condition among all other tested genotypes. The results of the investigation revealed that BINA Mung-6 (V8) genotype consistently scored the highest value for all parameters except for the root shoot ratio and water retention capacity that was statistically comparable to genotypes BARI Mung-4 (V2) and BINA Mung-5 (V7). Consistently poor performance were recorded from IPM-02-03 (V16) genotype which is statistically similar as genotypes BMXK1-09015-2 (V13) and BMXK1-09015-6 (V10). The maximum percentage of germination (98.12%), shoot length (139.40 mm), root length (99.07 mm), shoot dry weight (22.32 mg), root dry weight (6.88 mg), relative water content (94.78), water retention capacity (24.98), germination co-efficient (22.27) and vigor index (233.90) were reported from BINA Mung-6 (V8) at a concentration of 0 percent PEG. The minimum percentage of germination (28.22 percent), shoot length (31.17 mm), root length (16.50 mm), shoot dry weight (2.21 mg), root dry weight (0.97 mg), relative water content (25.55), water retention capacity (3.08), germination co-efficient (6.06) and vigor index (13.45) were reported from IPM-02-03 (V16) mungbean advance lines at 0 percent PEG. Maximum (0.92) root shoot ratio was recorded from both BARI Mung-8 (V6) and BMX-08011-2 (V11) mungbean genotypes at 20 percent PEG concentration and minimum (0.22) at 0 percent PEG concentration from BARI Mung-5 (V3) genotype. Maximum water retention capacity (74.45) was recorded at 20 per cent PEG concentration from IPM-02-03 (V16) genotype and minimum (5.22) was at 0 per cent PEG concentration from BINA Mung-6 (V8) genotype.

scholarly journals Chromium Toxicity in Sesbania sesban (L.) Merr

2014 ◽  
Vol 9 ◽  
pp. 66-75
Author(s):  
R. Bakiyaraj ◽  
T. Mahakavi ◽  
L. Baskaran
Keyword(s):  
Chlorophyll A ◽  
Dry Weight ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Proline Content ◽  
Sesbania Sesban ◽  
Fresh Weight ◽  
Root And Shoot Length ◽  
Chlorophyll A And B ◽  
Cr Concentration

Chromium is one of the most common toxic metals present in the environment that induces various toxic effects in plants. A pot experiment was conducted to determine the effects of chromium on germination percentage, seedling growth, chlorophyll ‘a’, ‘b’ and proline content of Sesbania sesban (L.) Merr. The seedlings were treated with different concentrations of control, 0.10, 0.25, 0.50, 0.75 and 1.00 g kg-1 of chromium. The parameters such as germination percentage, root and shoot length, seedling fresh weight dry weight, chlorophyll ‘a’, ‘b’ and proline content of leaves were measured. Our results indicated that a significant inhibitory effect was observed at all levels of chromium compared to control. Increasing the concentration of chromium to 1.00 g kg-1 showed a significant decrease in seed germination, shoot and root length, fresh weight, dry weight and chlorophyll ‘a’ and ‘b’ content of plant. While proline, catalase and peroxidase contents were increased by increasing Cr concentration. It was also noted that accumulation of chromium in the roots was much higher than the shoots of the seedlings under treatment.

scholarly journals The Effect of Priming Duration with Salicylic Acid under Salinity Stress on Growth and Leaf Anatomy of Sweet Corn (Zea mays L.)

2019 ◽  
Vol 3 (1) ◽  
pp. 36
Author(s):  
Krisnanda Surya Dharma ◽  
Maryani Maryani
Keyword(s):  
Salicylic Acid ◽  
Salinity Stress ◽  
Leaf Anatomy ◽  
Zea Mays L ◽  
Sweet Corn ◽  
Stomatal Density ◽  
Dry Weight ◽  
Germination Percentage ◽  
Proline Content ◽  
Fresh Weight

Salinity stress is known for adverse effect on plants. Priming with salicylic acid was able to improve plant performance under salinity stress. This study aimed to determine the effect of priming duration with salicylic acid on growth, leaf anatomy and the optimal priming duration for sweet corn seedlings (Zea mays L.) under salinity stress. The experiment was based on Completely Randomized Design with two factors and five replications. The first factor was priming duration with salicylic acid (2 mM) with four different durations (0, 12, 18 and 24 h). The second factor was the level of salinity (NaCl 0% and 3%). Parameters observed were germination percentage, plant height, root length, fresh weight, dry weight, chlorophyll content, leaf proline content, leaf anatomy and stomatal density. Data were analyzed with t-test, ANOVA and followed by Duncan’s test at 95% confidence level. The results showed that 18-h priming duration observed as the highest germination percentage which was 7% higher than control.  Priming for 24 h showed phytotoxic effect for sweet corn on the germination phase by reducing the percentage of germination. The application of salicylic acid mitigated the toxic effects of NaCl stress on maize seedlings and considerably improved root and shoot growth, photosynthetic pigments, fresh weight, dry weight, proline content, and stomatal density, as well as could maintaining the leaf anatomy. The optimal priming duration with salicylic acid for sweet corn seedlings under 3 % salinity was 18 h.

scholarly journals Effect of PEG Induced Drought Stress on Seed Germination and Seedling Growth of Greengram Genotypes

2021 ◽  
pp. 79-90
Author(s):  
A. Priya Dharshini ◽  
V. Babu Rajendra Prasad ◽  
K. Vanitha ◽  
N. Manivannan
Keyword(s):  
Drought Stress ◽  
Dry Weight ◽  
Germination Percentage ◽  
Proline Content ◽  
Seedling Mortality ◽  
Growth Indices ◽  
Peg 6000 ◽  
Drought Tolerant ◽  
Severe Constraint ◽  
High Level

Greengram (Vigna radiata L. Wilczek) is the third most important pulse crop and drought is the most severe constraint to greengram growth and productivity. The present study was conducted to identify the drought tolerant greengram genotypes. Four greengram varieties used for standarization of drought stress using Polyethyleneglycol (PEG) 6000. The effect of water stress caused by different concentration of PEG 6000 are control (0 MPa), -0.4MPa, -0.5 MPa, -0.6MPa and -0.7 MPa. Increasing PEG concentration decrease the germination percentage, root length, shoot length, fresh weigh and dry weight of seedlings. At -0.5 MPa shows 50% seedling mortality , So control and -0.5 MPa level of drought stress was used for screening the greengram genotypes. Under PEG induced drought situations, parameters such as germination percentage, growth indices and proline content were recorded in all greengram genotypes. Compared to control, PEG induced drought stress (-0.5MPa) decrease all these parameters studied, where as drought has increased the proline content in all greengram genotypes screened. Among the greengram genotypes VGG17019 and VGG17004 posses higher germination percentage, GSI and proline content indicates high level of tolerance to drought stress.

scholarly journals In vitro Osmotic Stress Memory in Chrisanthemum hybridum: Structural and Physiological Responses

2017 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lidiia Samarina ◽  
Valentina Malyarovskaya ◽  
Yulija Abilfazova ◽  
Natalia Platonova ◽  
Kristina Klemeshova ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Relative Water Content ◽  
Soluble Protein ◽  
Growth Parameters ◽  
Physiological Responses ◽  
Proline Content ◽  
Stress Exposure ◽  
Stress Memory ◽  
Relative Water

Structural and physiological responses of chrysanthemum to repeated osmotic stress were studied. Plants were cultured for 2 weeks (for each stress1 and stress 2) on half MS supplemented with mannitol 100 mM (Treatment I) and 200 mM (Treatment II). First stress inhibited growth parameters stronger than second stress in treatment I. In treatment II both stress events strongly inhibited growth parameters of micro‐shoots. Proline content exceeded control 6 ‐ 8 times after 1st stress, and 2 ‐ 5 times after the 2nd stress in treatments I and II, respectively. Soluble protein was accumulated in leaves during both stress exposures, and 2 ‐ 2.5 times exceeded control after the 2nd stress. Relative water content in both treatments increased after the 2nd stress exposure. In treatment II chlorophyll а and carotenoids contents were 8.78 and 4.62 mg/g comparing to control (4.21 and 2.25 mg/g, respectively) after the 1st stress. But after the 2nd stress there was no difference with control.Plant Tissue Cult. & Biotech. 27(2): 161-169, 2017 (December)

Penconazole and calcium ameliorate drought stress in canola by upregulating the antioxidative enzymes

2020 ◽  
Vol 47 (9) ◽  
pp. 825 ◽  
Author(s):  
Maryam Rezayian ◽  
Vahid Niknam ◽  
Hassan Ebrahimzadeh
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Antioxidative Enzymes ◽  
Proline Content ◽  
Fresh Weight ◽  
Negative Effects ◽  
Mda Content ◽  
Effects Of Drought

The aim of this research was to gauge the alternations in the lipid peroxidation and antioxidative enzyme activity in two cultivars (cv. RGS003 and cv. Sarigol) of canola under drought stress and drought tolerance amelioration by penconazole (PEN) and calcium (Ca). Plants were treated with different polyethylene glycol (PEG) concentrations (0, 5, 10 and 15%) without or with PEN (15 mg L–1) and Ca (15 mM). The Ca treatment prevented the negative effects of drought on fresh weight (FW) in RGS003 and Sarigol at 5 and 15% PEG respectively. Ca and PEN/Ca treatments caused significant induction in the proline content in Sarigol at 15% PEG; the latter treatment was accompanied by higher glycine betaine (GB), lower malondialdehyde (MDA) and growth recovery. Hydrogen peroxide (HO2) content in Sarigol was proportional to the severity of drought stress and all PEN, Ca and PEN/Ca treatments significantly reduced the H2O2 content. PEN and PEN/Ca caused alleviation of the drought-induced oxidative stress in RGS003. RGS003 cultivar exhibited significantly higher antioxidative enzymes activity at most levels of drought, which could lead to its drought tolerance and lower MDA content. In contrast to that of Sarigol, the activity of catalase and superoxide dismutase (SOD) increased with Ca and PEN/Ca treatments in RGS003 under low stress. The application of PEN and Ca induced significantly P5CS and SOD expression in RGS003 under drought stress after 24 h. Overall, these data demonstrated that PEN and Ca have the ability to enhance the tolerance against the drought stress in canola plants.

scholarly journals Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

2020 ◽  
Vol 9 (1) ◽  
pp. 706-714
Author(s):  
Muhammad Ikram ◽  
Naveed Iqbal Raja ◽  
Bilal Javed ◽  
Zia-ur-Rehman Mashwani ◽  
Mubashir Hussain ◽  
...  
Keyword(s):  
Drought Stress ◽  
Field Capacity ◽  
Dry Weight ◽  
Positive Control ◽  
Morphological Characterization ◽  
Leaf Length ◽  
Selenium Nanoparticles ◽  
Fresh Weight ◽  
Wheat Varieties ◽  
Shoot Dry Weight

AbstractThe present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.

Assessing allelopathic potential of Sonchus oleraceus L. (milk thistle) on germination and seedling growth of Oryza puncta

2021 ◽  
Vol 27 (1) ◽  
pp. 1-12
Author(s):  
Bilal Ahmad Khan ◽  
Saima Anwar ◽  
Rizwan Maqbool Maqbool ◽  
Muhammad Mohsin Amin ◽  
Muhammad Mansoor Javaid ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Seedling Growth ◽  
Dry Weight ◽  
Germination Percentage ◽  
Aqueous Extracts ◽  
Emergence Time ◽  
Fresh Weight ◽  
Water Control ◽  
Sonchus Oleraceus ◽  
Oryza Punctata

ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory ABSTRACT Aqueous extracts of plants are used to control weeds and having no hazards to environment and are inexpensive. Present study was undertaken to investigate the effect of aqueous extracts of Sonchus oleraceus L. on the Oryza punctata L. emergence and initial seedling growth. It consists of aqueous extract of leaves, stem and fruit of S. oleraceus at varying concentrations 0.25, 0.50, 1, 2, 4 and 8 % (w/v) along with a distilled water control. The allelochemicals present in the aqueous extracts showed stimulatory, inhibitory and hormetic responses depending upon the concentration and the plant part. Results directed that maximum mean emergence time (5.26 days) and minimum germination index (1.67), germination percentage (40%), seedling fresh weight (59 g) and dry weight (8 g) of O. punctata were examined with 8% aqueous extract of S. oleraceus fruit. However, 4% aqueous extract of S. oleraceus fruit produced minimum root length (5.71). On the basis this experiment it was concluded that 8% aqueous extract of S. oleraceus fruit can be used for controlling O. punctata weed. Keywords: Aqueous extract, environment, allelochamicals, hermetic response, stimulatory

scholarly journals In vitro assessment of wheat tolerance to drought

Genetika ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Vladislava Galovic ◽  
Zorana Kotaranin ◽  
Srbislav Dencic
Keyword(s):  
Drought Stress ◽  
Nutrient Medium ◽  
Callus Tissue ◽  
Geographic Origin ◽  
Control Group ◽  
Zygotic Embryos ◽  
Fresh Weight ◽  
Effects Of Drought ◽  
In Vitro Effects

Analyzed in this paper were the in vitro effects of drought stress in 13 genotypes of winter wheat, one genotype of spring wheat, and three Triticale genotypes of different geographic origin. Callus tissue was induced from immature zygotic embryos (10-15 days after pollination) on a modified MS nutrient medium. After two weeks, callus tissue was transplanted onto the same medium enriched with 5% high-molecular polyethylene glycol (PEG 6000), which was used as the stress agent to produce the effect of drought chemically. A control group of calluses was grown on an identical medium but without PEG. After four weeks of growing calluses on these mediums, we assessed callus mass survival ability of the genotypes before the transplantation as well as percentage reduction of callus fresh weight after the transplantation onto the nutrient medium with 5% PEG. Statistically significant differences were found among the genotypes in their response to the induced stress. The best survival ability before the transplantation was found in the genotype Mexicol20 (83%), while the lowest was recorded in Slavija (11.3%). Culture growing under stress conditions significantly reduced callus fresh weight in all of the genotypes. The lowest decrease of the callus mass relative to control was recorded in Rozofskaja (14.4%) and the highest in Miranovska (58.4%), indicating the genotypes' tolerance levels towards drought stress.

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