scholarly journals Screening of Mungbean Genotypes under Polyethylene Glycol (PEG) Induced Drought Stress Condition

Author(s):  
Abdullah All Imtiaz ◽  
Saleh Ahmed Shahriar ◽  
Md. Abdullahil Baque ◽  
Most. Nurjahan Khatun Eaty ◽  
Maliha Rahman Falguni

Sixteen advance genotypes of mungbeans under 5 different concentrations of Polyethylene Glycol (PEG) were studied to find out the better cultivar against drought stress condition. The experiment results revealed that germination, seedling production and water-related behavior of mungbean genotypes differed significantly under different PEG (drought inducer) concentrations. The mungbean genotype BINA Mung-6 (V8) is proved as highly tolerant against drought stress condition among all other tested genotypes. The results of the investigation revealed that BINA Mung-6 (V8) genotype consistently scored the highest value for all parameters except for the root shoot ratio and water retention capacity that was statistically comparable to genotypes BARI Mung-4 (V2) and BINA Mung-5 (V7). Consistently poor performance were recorded from IPM-02-03 (V16) genotype which is statistically similar as genotypes BMXK1-09015-2 (V13) and BMXK1-09015-6 (V10). The maximum percentage of germination (98.12%), shoot length (139.40 mm), root length (99.07 mm), shoot dry weight (22.32 mg), root dry weight (6.88 mg), relative water content (94.78), water retention capacity (24.98), germination co-efficient (22.27) and vigor index (233.90) were reported from BINA Mung-6 (V8) at a concentration of 0 percent PEG. The minimum percentage of germination (28.22 percent), shoot length (31.17 mm), root length (16.50 mm), shoot dry weight (2.21 mg), root dry weight (0.97 mg), relative water content (25.55), water retention capacity (3.08), germination co-efficient (6.06) and vigor index (13.45) were reported from IPM-02-03 (V16) mungbean advance lines at 0 percent PEG. Maximum (0.92) root shoot ratio was recorded from both BARI Mung-8 (V6) and BMX-08011-2 (V11) mungbean genotypes at 20 percent PEG concentration and minimum (0.22) at 0 percent PEG concentration from BARI Mung-5 (V3) genotype. Maximum water retention capacity (74.45) was recorded at 20 per cent PEG concentration from IPM-02-03 (V16) genotype and minimum (5.22) was at 0 per cent PEG concentration from BINA Mung-6 (V8) genotype.

2015 ◽  
Vol 18 (1) ◽  
pp. 81-88 ◽  
Author(s):  
MI Hossain ◽  
MA Mannan ◽  
MA Karim

A pot experiment was carried out under semi-controlled environmental condition in the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh during December 2012 through March 2013 aiming to alleviate the salinity stress effects on chickpea using salicylic acid (SA) and gibberelic acid (GA3). Chickpea variety BARI Chola-5 was used in the experiment. Salt solution was prepared by adding tap water in sea water to make 5, 7.5 and 10 dS m-1 salinity level. Plants were irrigated with 5, 7.5 and 10 dSm-1 concentrations of saline water from 14th days after sowing (DAS) to maturity (100 DAS) and control plants were irrigated with tap water. Different concentration of SA (200 ppm and 400 ppm) and GA3 (10 ppm and 20 ppm) were applied as foliar spray once in a week from 20 DAS to flowering stage. The data for chlorophyll content in leaf and water relation traits such as relative water content (RWC) and water retention capacity (WRC) were measured 7 days after foliar spray of plant growth regulators at flowering stage. Total dry weight (root+shoot), yield and yield contributing characters were measured at maturity. Results indicated that salinity decreased total dry weight, chlorophyll content, relative water content, water retention capacity and yield of chickpea. Foliar application of SA and GA3 at different doses under different salinity conditions had the positive effects related to mitigation of salinity stress effect but low concentration i.e., SA and GA3 @ 200 ppm and 10 ppm, respectively were found to alleviate the adverse effects significantly on the above parameters at low salinity condition (5 dSm-1).Bangladesh Agron. J. 2015, 18(1): 81-88


2017 ◽  
Vol 1 (2) ◽  
pp. 69-77
Author(s):  
EDI PURWANTO ◽  
SAMANHUDI SAMANHUDI ◽  
YONIAR EFFENDI

Purwanto E, Samanhudi, Yoniar Effendi Y. 2017. Response of some upland rice varieties to drought stress. Trop Drylands 1: 69-77. The study aimed to examine the response of upland rice varieties at germination stage on several PEG (Polyethylene Glycol) 6000 concentration levels and to determine growth characteristics, physiological changes and yield of upland rice varieties under drought stress condition in a pot experiment. The study consisted of two experiments; the first was to study germination and early growth, and the second was to study the vegetative and generative growth of upland rice under drought stress condition. Experiments I and II were conducted in a completely randomized factorial design consisted of two factors and three replications. In experiments I, the first factor was upland rice variety comprised of 10 varieties and the second factor was PEG concentration consisted of four levels. In experiment II, the first factor was upland rice varieties that responded the best to drought stress in experiment I, and the second factor was the drought stress treatment consisted of moisture content at 100, 75, 50 and 25% field capacity. The research was conducted at the Laboratory of Plant Physiology and Biotechnology and the greenhouse of Faculty of Agriculture, Sebelas Maret University (UNS) Surakarta. The results showed that the germination and vigor index decreased with increasing levels of PEG concentration. PEG concentration of 25 g L-1 of water was the most optimum for screening drought resistant upland rice. In experiment I, Towuti, Situ Patengang, Kalimutu and Gajah Mungkur varieties showed the best response to drought stress. Increased intensity of drought stress resulted in a reduction of plant height, number of tillers, dry weight, relative growth rate, leaf area, number of grains hill-1, 1000 grain weight, dry grain weight hill-1 and root dry weight. The drought stress also caused an earlier flowering date and increased percentage of empty grain. Based on the dry grain weight hill-1, Towuti exhibited a higher drought resistance level than Gajah Mungkur, Situ Patenggang and Kalimutu.


2015 ◽  
Vol 725-726 ◽  
pp. 355-360 ◽  
Author(s):  
Vitaly Terleev ◽  
Vladimir Badenko ◽  
Inna Guseva ◽  
Wilfried Mirschel

New theoretical justification for the function of soil differential moisture capacity (dependence of the relative water volume content on the capillary pressure) and its antiderivative is presented. New method is based on the concept of capillarity and the lognormal distribution of the effective radii of pores. Relative hydraulic conductivity of soil is calculated with usage of these functions and Mualem's approach. Hydrophysical parameters have been interpreted and evaluated on the base of some physical and statistical soil characteristics. Also the approximation for functions of water-retention capacity and relative hydraulic conductivity of soil has been proposed.


2019 ◽  
Vol 76 ◽  
pp. 111-123
Author(s):  
Ertran Yildirim ◽  
Melek Ekinci ◽  
Raziye Kul ◽  
Metin Turan ◽  
Ayla Gür

The study was conducted to determine effects of a new synthesis of methylamine on the plant growth, physiological and biochemical characteristics in pepper.  There were four irrigation levels [full irrigation (100%) (I0), 80% (I1), 60% (I2) and 40% (I3)] and two methylamine (MA) treatments (0, 2.5 mM). At the end of the study, it was observed that there were significant differences between applications and levels. Effects of MA treatments on plant growth (plant height, stem diameter, fresh, dry weight etc.), plant physiological and biochemical parameters [tissue electrical conductivity (TEC), tissue relative water content (TRWC), hydrogen peroxide (H2O2), malondialdehyde (MDA), proline, antioxidant enzyme activity], and plant nutrient element content of pepper seedlings under different irrigation levels were significantly important.  The results of the study showed that the drought stress conditions negatively affected the plant growth, increased the content of TEC, H2O2 and MDA, and decreased the TRWC and  plant mineral content in pepper. However, MA application improved plant growth and decreased TEC, H2O2 and MDA content compared to control in pepper under drought conditions. MA treated plants at I3 had higher shoot fresh weight and shoot dry weight than non-treated plants by 12 and 20%, respectively.  In conclusion, MA application could mitigate the deleterious effects of the drought stress on the pepper seedlings.


2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Lukita Devy ◽  
Winda Nawfetrias

This research studied the growth, quantity and quality of ginger rhizome under drought stress condition. The drought stress condition was 60% of soil field capacity. This experiment was arranged in randomized complete block designed. The treatments were drought stress periods (6, 4, 2 and 0 week before harvesting) with six replications. The result showed that drought stress period affected the quantity (rhizome dry weight) and quality (gingerol content) of ginger. Rhizome dry weight was decreased with the increase of drought stress period, while gingerol content showed opposite trend.


Author(s):  
A.M. Khorshid ◽  
F.A. Moghadam ◽  
I. Bernousi ◽  
S. Khayamim ◽  
A. Rajabi

This study was carried out in the Agricultural Research Center of West Azerbaijan, Iran in 2016. In this research, variations in different physiological and yield traits measurement of total dry weight, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, relative water content, relative water loss, root length, leaf area, root/shoot ratio, specific leaf weigh, sodium content, potassium content and proline were investigated in normal and saline condition. The results indicated that in saline condition, total dry weight, root fresh weight, shoot dry weight, root/Shoot ratio, specific leaf weight, root length, proline and Na content were increased and the other traits were decreased. Study of correlation of traits showed that most significant difference between the two conditions was observed for the root/shoot ratio, so that, this trait has negative significant relation with total dry weight, shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, relative water content, leaf area, root length, specific leaf weight in saline condition, but in normal condition correlation is positive and significant only in the total dry weight, root fresh weight, shoot dry weight and root dray weight and was not significant in the other traits. Step-wise regression analysis for total dry weight as dependent variable revealed that in normal condition, root fresh weight, shoot fresh weight and Na content expound of 93.1% and in saline condition root fresh weight, root length, Na content and proline explicate of 81.3% of total variation exist in total dry weight. Therefore, it is suggested to consider different traits in breeding programs for normal and saline conditions.


2016 ◽  
Vol 27 (2) ◽  
pp. 110-118 ◽  
Author(s):  
UK Salma ◽  
F Khatun ◽  
MJH Bhuiyan ◽  
S Yasmin ◽  
TH Khan

The aim of the present study was to study physiological response of seven chickpea varieties viz. Binachola-2, Binachola-3, Binachola-4, Binachola-5, Binachola-6, Binachola-7 and Binachola-8 at different levels of drought stress induced by polyethylene glycol (PEG).  Five different concentrations (0, 20, 35, 50, 60 g/L) of PEG 6000 was added in MS medium to create five different levels of drought stress for  in vitro screening of drought tolerant chick pea varieties. Data were recorded on germination percentage, fresh weight, shoot length, root length, dry weight, turgid weight, relative water content (RWC) and proline content. The seven chickpea varieties differed significantly for different parameters in response to the drought stress. Binachola-2 and Binachola-7 showed the best performance for all the parameters studied. At the highest dose of PEG (60 g/l), in the most water deficient condition, fresh weight was recorded 0.59 g and 0.84 g, Shoot lengths of 2.10 cm and 3.75 cm, root lengths of 1.15 cm and 1.00 cm, turgid weight of 0.960 g and 0.970 g, dry weight of 0.13 g and 0.21 g, relative water contents (RWC) of 85.71% and 83.33% were recorded in Binachola-2 and Binachola-7, respectively. Increased proline content was recorded with the increasing level of PEG concentration. Proline content 0.533g/100g FW and 0.598g/100g FW were observed in Binachola-2 and Binachola-7, respectively under the influence of PEG at 60 g/l. Data recorded for Binachola-3, Binachola-4, Binachola-5, Binachola-6 and Binachola-8 for the studied parameters revealed that they showed susceptible response against higher drought stress level gen­erated by PEG. The data of this experiment revealed that, the accumulation of proline was significantly greater under drought stress. Thus, it is evident that Binachola-2 and Binachola-7 performed better against drought stress condition. These results of physiological and biochemical parameters may be utilized as a selection indicator for breeding program and used as a baseline for improvement of chickpea varieties in Bangladesh.Progressive Agriculture 27 (2): 110-118, 2016


Author(s):  
Agung Wahyu Susilo ◽  
Sobir Sobir ◽  
Adinda Wuriandani ◽  
Desta Wirnas

Drought stress can affect changes in physiological, morphological, biochemical,and molecular of plant. Plant in drought stress showed slower growthand development than in normal condition. This research aimed to determine the response of cocoa genotypes in seedling phase to drought stress in morphological and stomata character. This research conducted with split-plot design with main plot were water regimes (25% and 100% available water content). Eleven genotypes were used in this research consisted of six genotypes crosses and five genotypes parents. Variables observed were stem diameter, root volume, root length, leaf area, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, root/shoot ratio, and stomatal conductance. Drought stress decreased values associated with all observed morphological characters and stomata characters. Root/shoot ratio and stomatal conductance can be used to determine genotype with tolerance to drought. Sulawesi 3 x ICCRI 09 showed heighest in root/shoot ratio and stomatal conductance. Sulawesi 3 x ICCRI 09 can be used as candidate of plant material tolerant to drought.


Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 705-715
Author(s):  
Hojjat Hasheminasab ◽  
Mohammad Assad

Heritability and Genetic Gain are commonly used by plant breeders to estimate the accuracy of a selection index and used for measuring the response to selection. The aim of the present study was the genetic and phenotypic analyses of some physiological traits to identify the most reliable of them for selective breeding of pistachio cultivars under drought stress condition. Nineteen Pistachio (Pistacia vera L.) cultivars with wide range of tolerance to drought stress were collected from across the orchards of Rafsanjan (Iran?s center of pistachio cultivation) and were used in randomized complete block design with three replications under two environmental conditions (normal and water stress) in 2011-2012. The results of genetic analysis showed that high magnitude of phenotypic and genotypic coefficient of variation along with broad sense heritability and genetic gain were estimated in relative water protection (RWP), excised leaf water loss (ELWL), relative water content (RWC) and relative water loss (RWL) under drought stress condition, indicating that the inheritance of these traits can be mainly controlled by additive gene effects followed by reflecting the possibility of effective selection for genetic improvement of these traits. Path analysis revealed that RWP had the highest direct and RWL and WRC indirect effects on YSI. Principal component analysis indicated that selected indices were reliable for classification and identification of drought-tolerant pistachio cultivars and identified RWP, RWC and WRC as the best indicators for screening drought tolerant genotypes.


Author(s):  
Clara Lizarazo ◽  
Frederick Stoddard ◽  
Pirjo Mäkelä ◽  
Arja Santanen

Drought is one of the most important abiotic stresses that causes significant reductions in crop yield, and thushinders the food security of the growing world population. In consequence, it is urgent to select crops able toresist drought, maintain high yield and have a good nutritive content. The purpose of this project was toevaluate the responses of different accessions of Andean lupin to drought stress, and identify if there aresignificant differences in their physiological responses.To identify germplasm for further investigation, thirty accessions of Lupinus mutabilis Sweet. and oneaccession of L. albococcineus Hort. were screened in two sets, A of 15 and B of 16 accessions. From thesesets, four lines were chosen on the basis of extreme values in the measurements, and this set of four wasinvestigated in depth (set C). Water stress consisted of controlled water loss from the soil (at 2% per day).The screening techniques used were: leaf temperature, stomatal conductance, relative water content, waterpotential, ion membrane leakage, and shoot dry weight were measured and transpiration efficiency wascalculated. In addition, carbon isotope discrimination, root length, root dry matter, proline content andsoluble sugar content were included in only set C.Based on the results from set A and set B, accessions PI 457981, PI 457972, and AC 2792 were consideredas being drought resistant and PI 510572 was considered as drought sensitive. However, the results from setC showed that accession PI 510572 is able to adapt its cell membrane to the drought stress so that its ionleakage is less than in the other accessions, and in addition it has the ability to accumulate higherconcentrations of osmoprotectants.Thus, this survey identified that some accessions of Andean lupin were able to avoid drought stress throughstomatal traits and root traits, and others that were able to tolerate drought through the accumulation ofosmotically active substances. Consequently, there are good prospects for breeding of Andean lupin toimprove its drought resistance.


Sign in / Sign up

Export Citation Format

Share Document