scholarly journals Diversity of Gut Methanogens and Functional Enzymes Associated With Methane Metabolism in Smallholder Dairy Cattle

Author(s):  
Denis K. Ng'etich ◽  
Rawlynce C. Bett ◽  
Charles K. Gachuiri ◽  
Felix M. Kibegwa

Abstract Methane is a greenhouse gas with disastrous consequences when released to intolerable levels. Ruminants produce methane during gut fermentation releasing it through belching and/or flatulence. To better understand the diversity of methanogens and functional enzymes associated with methane metabolism in dairy cows, 48 samples; six rumen and 42 dung contents were collected and analyzed using a shotgun metagenomic approach. The results indicated archaea from 5 phyla, 9 classes, 16 orders, 25 families, 59 genera, and 87 species. Gut sites significantly contributed to the presence and distribution of various methanogens (P<0.01). The class Methanomicrobia was abundant in the rumen samples (~ 39%) and in dung (~44%). The most abundant (~17%) methanogen species identified was Methanocorpusculum labreanum. However, some taxonomic classes were not classified (~ 6% in the rumen and ~4% in the dung). Furthermore, five functional enzymes: Glycine/Serine hydroxy methyltransferase, Formylmethanofuran—tetrahydromethanopterin N-formyltransferase, Formate dehydrogenase, Anaerobic carbon monoxide dehydrogenase and Catalase-peroxidase were associated with methane metabolism. KO0600 module and Enzyme Commissions (1.11.1.6 & 2.1.2.1) were common for dung and rumen fluid’s enzymatic pathways. Functional analysis for the enzymes identified were significant (P<0.05) for 5 metabolism processes. Breeding for tolerable methane emitting dairy cattle for a sustainable environment should be undertaken.

2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.


2016 ◽  
Vol 52 ◽  
pp. 6-12 ◽  
Author(s):  
M. V. Gladiy ◽  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holyosa ◽  
A. V. Tuchyk ◽  
...  

The main goal of dairy breeds selection should be improving breeding and productive qualities of animals under modern conditions. The majority of farms, using native breeds to produce milk, has created optimal conditions for keeping and feeding, selection and matching, growing of replacements etc. Further improvement of created native dairy breeds for economically useful traits occurs at total use of purebred Holstein bulls (semen) of foreign selection. In order to realistically assess milk productivity (milk yield, fat content in milk and fat yield) of Ukrainian Black-and-White and Red-and-White Dairy cows should be conducted a comparative analysis of Holstein cows under the same conditions of feeding and keeping. It was established that Ukrainian Red-and-White Dairy cows were characterized by the highest milk yields for 305 days of all lactations, taken into account, the among three investigated breeds. Their milk yield during the first lactation was 5933 kg of milk, during the second – 6393 kg, the third – 6391 kg and during higher lactation – 6650 kg. Ukrainian Black-and-White Dairy cows were second by milk yield (except for the second lactation), during the first lactation – 5932 kg of milk, the third – 6462 kg and higher – 6541 kg, and Holstein cows were third, during the first lactation – 5794 kg of milk, the second – 6381 kg, the third – 6335 kg and higher – 6469 kg. The fat content was almost the same and varied within 3.49-3.58% in milk of Ukrainian Red-and-White Dairy cattle, 3.50-3.60% in milk of Ukrainian Black-and-White Dairy cattle and 3.50-3.56% in Holsteins’ milk. The difference between the breeds was within 0.01-0.04%. All the investigated breeds had predominance in fat yield for three lactations over standards of these breeds: Ukrainian Red-and-White Dairy cows from 75.1 to 93.4 kg, Ukrainian Black-and-White Dairy cows – 75.1-89.0 kg respectively and Holstein cows – 41.9-60.2 kg. It was found different level of positive correlation between milk yield and fat yield in all the cases and high correlation (r = 0.604-0.921, P < 0.001) in five cases (41.7%) Negative correlation coefficients indicate that selection of animals to higher milk yield in the herd will decrease the second trait – fat content in milk. Positive and highly significant correlation between milk yield and fat yield indicates that selection of cows in the herd to higher milk yields will increase fat yield. It was revealed that bulls were among the factors impacted the milk productivity (milk yield, fat content, fat yield) of three investigated breeds. So, the force (η²x) of father’s impact on milk yield was15.4-47.9%, fat content – 22.0-43.4% and fat yield – 14.9-47.7% taking into account a lactation and a breed. The force of lines impact (η²x) was second; it was on milk yield 6.1-24.5%, fat content – 4.1-17.1 and fat yield – 5.8-23.5%. The force of breeds impact (η²x) was last; it was on milk yield 0.3-2.9%, fat content – 0.2-0.3% and fat yield – 0.6-2.7%. So, the comparative studies of milk productivity of Ukrainian Red-and-White and Black-and-White Dairy cattle with Holsteins indicate that under similar conditions of feeding and keeping, these native breeds can compete with Holstein cattle. The milk yield for 305 days of higher lactation was 6650 kg of milk in Ukrainian Red-and-White Dairy cows, 6541 kg in Ukrainian Black-and-White Dairy cows and 6469 kg in Holsteins. It was found the inverse correlation r = -0.025-0.316 between milk yield and fat content in milk in most cases. Selection and matching of animals in the herd should be carried out simultaneously on these traits. It was found positive repeatability of milk yields between the first and second, the third and higher lactations (rs = 0.036-0.741), indicating the reliability of forecasting increase in milk productivity during the next lactations in all herd. Bulls have the greatest impact (η²x) on milk productivity among the factors taken into account: milk yield – 15.4-47.9%, fat content in milk – 22.0-43.4% and fat yield – 14.9-47.7%.


1953 ◽  
Vol 20 (1) ◽  
pp. 1-12 ◽  
Author(s):  
C. P. Cox

1. Some questions concerning the interpretation of observations on milking dairy cattle are considered.2. A formula is presented for analysing the contributions of changes in the numerator and denominator to the changes in a percentage or fraction, and the formula is applied to changes in the composition of milk.3. The depressions of the butterfat and solids-not-fat percentages consequent on diets reduced in hay in a dairy-cattle feeding experiment are examined with this constituent analysis.The contributions of changes in the magnitudes of the aqueous, butterfat and solids-not-fat constituents to the difference between the butterfat percentages of cows on two treatments, 18 lb. hay/day (control) and 6 lb. hay /day, are about equal. There is an apparent qualitative difference between the effects of the two non-control treatments, and a reduced yield of butterfat would alone largely account for the lower butterfat percentage of cows on the 2 lb. hay /day treatment.


2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.


1970 ◽  
Vol 6 ◽  
pp. 91-96
Author(s):  
M Saiful Islam ◽  
Susanta Kumar Kundu

Impact of genotypes and parity on some vital reproductive and productive attributes in the local (L×L, n = 100) and four crossbred cows (L×F, L×SL, L×JR and L×S; n = 318) raised in randomly selected smallholder dairy farms scattered all over Natore District and adjacent areas have been assessed during a period from September 2007 to June 2010. With regard to reproductive attributes, significant differences existed among the cattle genotypes (P<0.05) except for gestation length (GL) and age at weaning (AW). The lowest age at puberty (AP) was found for L×F (21.42±0.37 months), while the highest for L×L (31.67±0.74 months). In terms of productivity, L×F cows produced the highest daily milk yield (DMY; 6.22±0.13 L), coupled with the highest total lactation yield (TLY; 2163.43±47.77 L), while L×L produced the lowest values (1.49±0.04 L and 416.40±12.3 L, respectively) for the traits. The effect of parity on both reproductive and productive attributes showed that the middle-aged dairy cows of the 3rd and 4th parities performed better than the younger (1st and 2nd parities) or the older (5th and beyond) ones. Considering the overall performance, the L×F cows could be ranked as the best genotype followed by their L×SL, L×JR, L×S and L×L counterparts in the study area. DOI: http://dx.doi.org/10.3329/jles.v6i0.9727 JLES 2011 6: 91-96


2016 ◽  
Vol 1 (1) ◽  
pp. 709-718
Author(s):  
Muhammad Junaidi ◽  
Cut Intan Novita ◽  
Dzarnisa Dzarnisa

Abstrak. Suatu penelitian telah dilaksanakan di desa Suka Mulya, Kecamatan Lembah Seulawah, Kabupaten Aceh Besar. Penelitian berlangsung dari bulan November sampai Desember 2016. Penelitian ini bertujuan untuk mengetahui kondisi faali sapi Peranakan Fries Holland (PFH) di peternakan rakyat desa Suka Mulya Kecamatan Lembah Seulawah Kabupaten Aceh Besar. Metode penelitian ini bersifat deskriptif analitis dengan mengunakan metode survei dan observasi langsung ke peternak dan sapi perah PFH. Lokasi penelitian ditentukan secara purposive sampling. Penetapan responden ditentukan mengunakan metode random sampling, jumlah responden sebanyak 10 peternak yang memelihara sapi perah. Materi yang digunakan dalam penelitian ini adalah 13 ekor sapi perah PFH. Parameter penelitian meliputi frekuensi pernafasan, denyut jantung dan suhu rektal. Data pendukung yang diperlukan dalam penelitian ini meliputi suhu dan kelembaban kandang, manajemen pemeliharaan sapi perah, kesehatan ternak, dan karakteristik peternak/responden. Hasil penelitian menunjukkan bahwa rataan frekuensi pernafasan sapi PFH adalah28,03 ± 0,63 kali/menit, frekuensi denyut jantung 68,92 ± 0,71  kali/menit dan suhu rektal 36,57 ± 0,55 °C. Dari hasil penelitian ini dapat disimpulkan bahwa kondisi faali/fisiologis ternak perah yang ada di peternakan Rakyat Desa Suka Mulya dalam keadaan baik/normal. Study on Condition of Dairy Cattle Breed Physiologic Fries Holland (PFH) Livestock in the Village People Prefer Mulya Seulawah Valley District District of Aceh Aceh Province Abstract. This study aimed to determine the effect of dairy cattle rearing system to physiological conditions in the district of Aceh Besar district seulawah Valley. The method used is descriptive analysis using the method of survey and direct observation to farmers and dairy cows PFH measurement of physiological conditions/physiological. This research by using two stages, the first stage is the observation field conditions and the second stage is the collection of data. Observation phase field conditions to determine the location of the study and the sample size, the data collection phase aims to obtain primary data and secondary data. The research location determined by purposive Desa Suka Mulya. Sampling of cattle that took cattle PFH to measure physiologic conditions/physiological. The number of samples taken in this study there are few dairy cows. The data collected from these samples include respiratory rate, heart rate, rectal temperature. Results of this study was the frequency of breathing 28,03 ± 0,63 times per minute, heart rate 68,92 ± 0,71 times per minute, a rectal temperature of 36,57 ± 0,55 times per minute. From the results of this research can be concluded that the physiologic conditions/physiological dairy cattle farm in the village of Suka Mulya People in a good state/normal


2008 ◽  
Vol 24 (1-2) ◽  
pp. 25-35 ◽  
Author(s):  
Slavca Hristov ◽  
B. Stankovic ◽  
Z. Zlatanovic ◽  
M. Joksimovic-Todorovic ◽  
V. Davidovic

This paper provides an overview of recent developments in rearing conditions, health and welfare issues of dairy cows. The last approximately 30 years has witnessed worldwide increasing scientific research, consumer activity, and political response towards housing condition, health and welfare issues of dairy cattle. All buildings and housing systems for dairy cattle should be designed, constructed, maintained and managed to assist in the achievement of the Five Freedoms: freedom from hunger and thirst, freedom from discomfort, freedom from pain, injury and disease, freedom to express normal behavior and freedom from fear and distress. Whether dairy cows are housed in cubicles, straw yards or cow sheds, in order to maximize their performance and to ensure satisfactory standards of welfare, the accommodation must provide the most basic behavioral and physiological needs. As an absolute minimum, the housing must provide a comfortable, clean, well drained and dry lying area together with shelter from adverse weather. Also, it must allow the cow to move freely around without risk of injury and certain diseases. If the housing system does not provide for these basic needs, then not only will health, welfare and production of dairy cattle be compromised, but it is likely that failure to comply with the welfare codes and the law relating to animal welfare will occur.


2007 ◽  
Vol 2007 ◽  
pp. 148-148
Author(s):  
A. Heravi Moussavi ◽  
M. Danesh Mesgaran ◽  
T. Vafa

Reproduction and milk production are the principal factors that are influencing dairy farm profitability. The dairy industry in Iran has changed dramatically in the last decade. The shift toward more productive cows and larger herds in Iran is associated with a decrease reproductive efficiency (Heravi Moussavi et al., 2004). Increased knowledge about the principal causes of reduced fertility is essential. The root cause of the declining fertility is probably a combination of a variety of physiological and management factors that have an additive effect on reproductive efficiency. Dairy cattle are inseminated and pregnancy is established while dairy cows are lactating. Based on the analyses of large datasets, there is clearly an antagonistic relationship between milk production and reproduction in dairy cattle (Lucy, 2001). It was shown that the hazard ratio for cumulative first 60-day milk yield and conception in high producer cows was 8 percent less than the others and also high milk yield was a risk factor for several reproductive disorders (Grohn and Rajala-Schultz, 2000). The objective of this study was to evaluate the effect of first 60-day cumulative milk yield on days open in Iranian Holstein dairy cows.


1997 ◽  
Vol 60 (11) ◽  
pp. 1386-1387 ◽  
Author(s):  
DANIEL H. RICE ◽  
ERIC D. EBEL ◽  
DALE D. HANCOCK ◽  
THOMAS E. BESSER ◽  
DONALD E. HERRIOTT ◽  
...  

Cull dairy cattle both on the farm and at slaughter from herds in the states of Idaho, Oregon, and Washington were surveyed for Escherichia coli O157 by culturing fecal swab samples. A total of 205 cull cows from 19 dairy herds were sampled on the farm of origin; 7 (3.4%) tested positive for E. coli O157. A total of 103 cull cows from 15 dairy herds were sampled at slaughter; 4 (3.9%) were positive for E. coli O157. Eighty-nine cull cows were sampled both at the farm and at slaughter; 2 (2.2%) were positive in both locations, 3 (3.3%) only on the farm, and 2 (2.2%) only at the slaughter plant. Seven (7.9%) of the 89 cull cows tracked from farm to slaughter were positive in at least one location. This suggests a higher prevalence of E. coli O157 in cull dairy cattle than previously has been reported to occur in other ages and classes of cattle.


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