scholarly journals Identification And Expression Analysis of FORMIN Gene Family In Rice (Oryza Sativa)

2021 ◽  
Author(s):  
Bin Li ◽  
Zhi-Ye Du ◽  
Shan He ◽  
Kai Xiao ◽  
Xing Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Protein Protein Interaction ◽  
Subcellular Localization Prediction ◽  
Cytoskeleton Dynamics ◽  
Ppi Prediction ◽  
First Time ◽  
Silico Analysis ◽  
Localization Prediction ◽  
Shed Light ◽  
Hormone Signals

Abstract Background: FORMIN proteins, which are composed of proteins containing FH1 and FH2 domains, play crucial roles in the growth and development of organisms. However, the functions of FORMINs in rice (Oryza sativa) remain largely unclear. Results: In this study, a total of 17 FORMIN genes were identified in rice, OsFH17 was the first time identified in this study. In addition, the distribution on chromosomes, gene structure, as well as conserved motifs of rice FORMINs was investigated. According to their protein structural and phylogenetic features, these 17 rice FORMIN genes were classified into two distinct subfamilies. Subcellular localization prediction showed that rice FORMINs were located in cytosol, golgi, endoplasmic reticulum, extracellular, and vacuole. Protein protein interaction (PPI) prediction results shown that FORMIN protein might answer hormone signals and be involved in cytoskeleton dynamics regulation and cell wall morphology regulation. The results of silico analysis and qRT-PCR confirmation of the gene expression showed that the expression of rice FORMINs were related to their tissue distribution. Moreover, OsFH3, OsFH5 and OsFH7 were upregulated under phytohormone treatments. Conclusions: Overall, our research may shed light on the understanding and further investigation of the biological functions of rice FORMINs.

Aleksey Peshkov as an editor of Samarskaya Gazeta

2020 ◽  
pp. 128-138
Author(s):  
A. S. Bik-Bulatov
Keyword(s):  
Local History ◽  
Organizational Skills ◽  
First Time ◽  
New Evidence ◽  
Insight Into ◽  
Shed Light

The article uses little known letters of M. Gorky, many of which were published for the first time in 1997, as well as findings of Samara-based experts in local history to shed light on the writer’s work as editor-in-chief of the Samarskaya Gazeta newspaper in 1895. The researcher introduces hitherto unstudied reminiscences of the journalist D. Linyov (Dalin) about this period, which reference a letter by Gorky, now lost. The paper details a newly discovered episode of Gorky’s professional biography as a journalist: it concerns his campaign against a Samara ‘she-wolf,’ the madam of a local brothel A. Neucheva. Linyov’s reminiscences turn out to be an important and interesting source, offering an insight into the daily grind of the young editor Gorky, providing new evidence of his excellent organizational skills, and describing his moral and social stance. The author presents his work in the context of a recently initiated broader discussion about the need to map out all Russian periodicals for the period until 1917, as well as all research devoted to individual publications.

[I] ‘did write this Wyll with my own hand’

2020 ◽  
Vol 32 (4) ◽  
pp. 66-77
Author(s):  
Vassiliki Markidou
Keyword(s):  
Early Modern ◽  
Urban Landscape ◽  
Nation Building ◽  
Early Modern London ◽  
First Time ◽  
Shed Light

This article attempts for the first time to shed light on the politics of simulation and dissimulation in Isabella Whitney’s ‘Wyll and Testament’. It also argues that the poem both reflects its creator’s awareness of the celebrated English historical and topographical narratives and deviates from them by crucially omitting a seminal part of London’s history, namely its Troynovant tradition. In so doing, as well as by defining a paradoxical urban landscape, Whitney presents a tale not of the (mythic) founding of the English capital with its patriarchal and nation-building connotations, but of its (satiric) bequeathal by benevolent femininity, as such offering its reader a different angle from which to explore and interpret early modern London.

scholarly journals Transcriptional landscape of cellular networks reveal interactions driving the dormancy mechanisms in cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dilara Uzuner ◽  
Yunus Akkoç ◽  
Nesibe Peker ◽  
Pınar Pir ◽  
Devrim Gözüaçık ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Regulatory Networks ◽  
Metastatic Cancer ◽  
Interaction Network ◽  
Current Treatment ◽  
Cellular Mechanisms ◽  
Protein Protein Interaction ◽  
Cancer Dormancy ◽  
Protein Protein Interaction Networks ◽  
First Time

AbstractPrimary cancer cells exert unique capacity to disseminate and nestle in distant organs. Once seeded in secondary sites, cancer cells may enter a dormant state, becoming resistant to current treatment approaches, and they remain silent until they reactivate and cause overt metastases. To illuminate the complex mechanisms of cancer dormancy, 10 transcriptomic datasets from the literature enabling 21 dormancy–cancer comparisons were mapped on protein–protein interaction networks and gene-regulatory networks to extract subnetworks that are enriched in significantly deregulated genes. The genes appearing in the subnetworks and significantly upregulated in dormancy with respect to proliferative state were scored and filtered across all comparisons, leading to a dormancy–interaction network for the first time in the literature, which includes 139 genes and 1974 interactions. The dormancy interaction network will contribute to the elucidation of cellular mechanisms orchestrating cancer dormancy, paving the way for improvements in the diagnosis and treatment of metastatic cancer.

scholarly journals Genome-wide identification of the GATA transcription factor family and their expression patterns under temperature and salt stress in Aspergillus oryzae

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chunmiao Jiang ◽  
Gongbo Lv ◽  
Jinxin Ge ◽  
Bin He ◽  
Zhe Zhang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Expression Patterns ◽  
Interaction Network ◽  
Protein Protein Interaction ◽  
Genome Wide ◽  
Gata Transcription Factor ◽  
Starting Point ◽  
Evolutionary Features ◽  
First Time

AbstractGATA transcription factors (TFs) are involved in the regulation of growth processes and various environmental stresses. Although GATA TFs involved in abiotic stress in plants and some fungi have been analyzed, information regarding GATA TFs in Aspergillusoryzae is extremely poor. In this study, we identified and functionally characterized seven GATA proteins from A.oryzae 3.042 genome, including a novel AoSnf5 GATA TF with 20-residue between the Cys-X2-Cys motifs which was found in Aspergillus GATA TFs for the first time. Phylogenetic analysis indicated that these seven A. oryzae GATA TFs could be classified into six subgroups. Analysis of conserved motifs demonstrated that Aspergillus GATA TFs with similar motif compositions clustered in one subgroup, suggesting that they might possess similar genetic functions, further confirming the accuracy of the phylogenetic relationship. Furthermore, the expression patterns of seven A.oryzae GATA TFs under temperature and salt stresses indicated that A. oryzae GATA TFs were mainly responsive to high temperature and high salt stress. The protein–protein interaction network of A.oryzae GATA TFs revealed certain potentially interacting proteins. The comprehensive analysis of A. oryzae GATA TFs will be beneficial for understanding their biological function and evolutionary features and provide an important starting point to further understand the role of GATA TFs in the regulation of distinct environmental conditions in A.oryzae.

scholarly journals Moral psychology of sex robots: An experimental study − how pathogen disgust is associated with interhuman sex but not interandroid sex

2020 ◽  
Vol 11 (1) ◽  
pp. 233-249 ◽  
Author(s):  
Mika Koverola ◽  
Marianna Drosinou ◽  
Jussi Palomäki ◽  
Juho Halonen ◽  
Anton Kunnari ◽  
...  
Keyword(s):  
Science Fiction ◽  
Experimental Studies ◽  
Sex Worker ◽  
Interest In Science ◽  
Sex Robots ◽  
Monogamous Relationship ◽  
Attitudes Toward Sex ◽  
First Time ◽  
And Robotics ◽  
Shed Light

AbstractThe idea of sex with robots seems to fascinate the general public, raising both enthusiasm and revulsion. We ran two experimental studies (Ns = 172 and 260) where we compared people’s reactions to variants of stories about a person visiting a bordello. Our results show that paying for the services of a sex robot is condemned less harshly than paying for the services of a human sex worker, especially if the payer is married. We have for the first time experimentally confirmed that people are somewhat unsure about whether using a sex robot while in a committed monogamous relationship should be considered as infidelity. We also shed light on the psychological factors influencing attitudes toward sex robots, including disgust sensitivity and interest in science fiction. Our results indicate that sex with a robot is indeed genuinely considered as sex, and a sex robot is genuinely seen as a robot; thus, we show that standard research methods on sexuality and robotics are also applicable in research on sex robotics.

scholarly journals An Application of Fit Quality to Screen MDM2/p53 Protein-Protein Interaction Inhibitors

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3174 ◽  
Author(s):  
Xin Xue ◽  
Gang Bao ◽  
Hai-Qing Zhang ◽  
Ning-Yi Zhao ◽  
Yuan Sun ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Drug Target ◽  
P53 Protein ◽  
Pharmacophore Model ◽  
Cellular Level ◽  
Complex Structures ◽  
Ligand Complex ◽  
Null Cell ◽  
Protein Protein Interaction ◽  
First Time

: The judicious application of ligand or binding efficiency (LE) metrics, which quantify the molecular properties required to obtain binding affinity for a drug target, is gaining traction in the selection and optimization of fragments, hits and leads. Here we report for the first time the use of LE based metric, fit quality (FQ), in virtual screening (VS) of MDM2/p53 protein-protein interaction inhibitors (PPIIs). Firstly, a Receptor-Ligand pharmacophore model was constructed on multiple MDM2/ligand complex structures to screen the library. The enrichment factor (EF) for screening was calculated based on a decoy set to define the screening threshold. Finally, 1% of the library, 335 compounds, were screened and re-filtered with the FQ metric. According to the statistical results of FQ vs activity of 156 MDM2/p53 PPIIs extracted from literatures, the cut-off was defined as FQ = 0.8. After the second round of VS, six compounds with the FQ > 0.8 were picked out for assessing their antitumor activity. At the cellular level, the six hits exhibited a good selectivity (larger than 3) against HepG2 (wt-p53) vs Hep3B (p53 null) cell lines. On the further study, the six hits exhibited an acceptable affinity (range of Ki from 102 to 103 nM) to MDM2 when comparing to Nutlin-3a. Based on our work, FQ based VS strategy could be applied to discover other PPIIs.

Proactive Quality Control: Observing System Simulation Experiments with the Lorenz ’96 Model

2019 ◽  
Vol 147 (1) ◽  
pp. 53-67 ◽  
Author(s):  
Tse-Chun Chen ◽  
Eugenia Kalnay
Keyword(s):  
Quality Control ◽  
Weather Prediction ◽  
Computational Cost ◽  
Network Size ◽  
Simulation Experiments ◽  
Systematic Testing ◽  
Kalman Gain ◽  
Lorenz 96 ◽  
First Time ◽  
Shed Light

Proactive quality control (PQC) is a fully flow-dependent QC for observations based on the ensemble forecast sensitivity to observations technique (EFSO). It aims at reducing the forecast skill dropout events suffered in operational numerical weather prediction by rejecting observations identified as detrimental by EFSO. Past studies show that individual dropout cases from the Global Forecast System (GFS) were significantly improved by noncycling PQC. In this paper, we perform for the first time cycling PQC experiments in a controlled environment with the Lorenz model to provide a systematic testing of the new method and possibly shed light on the optimal configuration of operational implementation. We compare several configurations and PQC update methods. It is found that PQC improvement is insensitive to the suboptimal configurations in DA, including ensemble size, observing network size, model error, and the length of DA window, but the improvements increase with the flaws in observations. More importantly, we show that PQC improves the analysis and forecast even in the absence of flawed observations. The study reveals that reusing the exact same Kalman gain matrix for PQC update not only provides the best result but requires the lowest computational cost among all the tested methods.

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