Antimicrobial And Synergistic Activity of The Drug Association of Allium Sativum And Antibiotics Against Group B Streptococcus

2022 ◽  
Author(s):  
Sônia Maria Rolim Rosa Lima ◽  
Maria Thereza Gamberini ◽  
Domingos Sávio Rodrigues ◽  
Pedro Ismael Silva Junior ◽  
Kátia Andrea de Menezes Torres
Keyword(s):  
Antimicrobial Activity ◽  
Group B Streptococcus ◽  
Neonatal Infection ◽  
Penicillin G ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
The Mean ◽  
Group B

Abstract Maternal colonization by Group B Streptococcus during pregnancy increases the risk of neonatal infection due to vertical transmission from mother to fetus before or during labor. The aims of this study were to evaluate the antimicrobial activity of SP80 (obtained from RGE) and its synergism associated with the antibiotic against strains of Streptococcus agalactiae. Biomonitoring of SP80 disclosed antimicrobial activity only in fractions F18, F19, F20 and F42. The broth microdilution was used to determine the antimicrobial activity of SP80 and fractions from SP80 and to establish the MIC of SP80 (2.40 mg/mL). By using the disk diffusion method, fifty-five clinical isolates of S. agalactiae and 1 ATCC were tested against the association of SP80 with antibiotic penicillin G and ampicillin, respectively, for synergistic assessment. The association of SP80 with penicillin G showed that the mean of the inhibition halos decreased, but it was not significant, with p<0.07. In contrast, the association of SP80 with ampicillin caused the mean inhibition halos to increase with a p<0.001, a significant result. SP80 has antimicrobial activity against S. agalactiae Gram-positive bacteria, and the association with the antibiotic ampicillin showed a synergistic effect, which did not occur when in association with penicillin G.

scholarly journals Synergistic effects of Persicaria odorata (Daun Kesom) leaf extracts with standard antibiotics on pathogenic bacteria

2020 ◽  
Vol 16 (2) ◽  
Author(s):  
P. M. Ridzuan ◽  
Hairul Aini Hamzah ◽  
Anis Shah ◽  
Norazian Mohd Hassan ◽  
Baharudin Roesnita
Keyword(s):  
Pathogenic Bacteria ◽  
Synergistic Effects ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Synergistic Activity ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Bacteria Growth ◽  
Gram Positive Bacteria

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH).  Each extract was applied on vancomycin (30µg), erythromycin (15µg) and gentamicin (10µg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 µg/ml to 100 µg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis.  The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.

scholarly journals Antimicrobial Activity of two Mentha Species Essential Oil and its Dependence on Different Origin and Chemical Diversity

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mária Pl'uchtová ◽  
Teresa Gervasi ◽  
Qada Benameur ◽  
Vito Pellizzeri ◽  
Daniela Grul'ová ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibition Zone ◽  
Chemical Diversity ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Growth Inhibition Zone

Genus Mentha presents group of plants which are the most studied in family Lamiaceae. Aboveground parts are used for different purposes in pharmacy, food industry or confectionery. Most important is natural product extracted from leaves - essential oil (EO). The aim of presented experiment was to demonstrate different chemotype and compare antibacterial activity of two Mentha species EO. Plant samples were obtained from various environments – from Slovakia and from Italy. Dominant compounds were determined by GC/MS. The results showed high amount of menthol and menthone in tested Slovak peppermint EO. On the other hand, carvone and 1,8-cineole were determinate as dominant compounds in Italian spearmint EO. The antimicrobial activity of the EO was investigated by disc diffusion and broth micro dilution methods. EO was evaluated for their antibacterial activity against 7 microorganisms: Enterobacter cloacae, Salmonella spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes. The determination results of antibacterial activity by agar disk diffusion method ranged from 7 to 14 mm of the growth inhibition zone. MIC of tested mint EO varied from 0.625 to 2.5 μg/mL. In addition, both EO showed relatively the same antibacterial activity against the selected Gram-negative bacteria. However, there is a variation in the antibacterial activity against Gram-positive bacteria.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

scholarly journals Comparison of Antimicrobial Activity ofAllium sativumCloves from China and Taşköprü, Turkey

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Ali Yetgin ◽  
Kerem Canlı ◽  
Ergin Murat Altuner
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

In this study, antimicrobial activities of two different samples ofAllium sativumL. from Turkey (TR) (Taşköprü, Kastamonu, Turkey) and China (CN) were determined. A broad spectrum of Gram-negative and Gram-positive bacteria (17 bacteria) including species ofBacillus,Enterobacter,Enterococcus,Escherichia,Klebsiella,Listeria,Pseudomonas,Salmonella, andStaphylococcuswere used for testing antibacterial activity. In addition, antifungal activity againstCandida albicanswas also investigated. Antimicrobial activity was tested by using 3 different processes (chopping, freezing, and slicing by the disk diffusion method). The results showed that TR garlic presented more antimicrobial activity than CN garlic. Mechanism of activity of CN garlic could be proposed to be different from that of TR garlic.

Antimicrobial activity of three species of Phyllanthus (quebra-pedra) and its commercial product

2009 ◽  
Vol 4 (1) ◽  
pp. 93
Author(s):  
Tássia Campos de Lima Silva ◽  
Jorge Veras Filho ◽  
Janete Magali Araújo ◽  
Ulysses Paulino Albuquerque ◽  
Vânia Teixeira Lima ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Medicinal Species ◽  
Phyllanthus Niruri ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Therapeutic Value ◽  
Antibacterial And Antifungal

ABSTRACT Objective: to determine the antimicrobial activity of three plant species of the genus Phyllanthus (P. amarus Schum. and Thom., P. tenellus Roxb. and Phyllanthus niruri L.) and its commercial dye (phytotherapic), opposite the bacteria and yeasts. Methods: we evaluated the antimicrobial activity against microorganisms by disk diffusion method, and then evaluated minimum inhibitory concentration of the best results from the microdilution method. Results: the medicinal species have antimicrobial activity to Gram-positive bacteria. The commercial dye showed significant antimicrobial activity. The extracts showed the best activity for S. aureus (P. amarus with MIC 0.16 mg / mL and P. tenellus with MIC 0.10 mg / mL), opposite to B. subtilis results were also satisfactory (P. amarus 0.25 mg / mL and P. tenellus 0.30 mg / mL). The commercial dye showed MIC greater than 1mg/ml only for S. aureus with MIC of 0.70 mg / mL, and for B. subtilis showed MIC 1.10 mg / mL. With respect to the yeast, all substances were less significant than for gram-positive bacteria, ranging from 0.70 to 1mg/ml for crude extracts and 1.10 to 1.20 mg / mL for the dyeing trade. Conclusion: this study showed the potential antibacterial and antifungal therapeutic value of these species can be better evaluated. Descriptors: Phyllanthus, MIC, antimicrobial, fungicide. RESUMOObjetivo: determinar a atividade antimicrobiana de três espécies de plantas do gênero Phyllanthus (P. amarus Schum e Thom.,  P. tenellus Roxb. e Phyllanthus niruri L.) e de sua tintura comercial (fitoterápico), frente a bactérias e leveduras. Métodos: foi avaliada a atividade antimicrobiana frente a microrganismos através do método de disco difusão, em seguida foram avaliadas as concentrações mínimas inibitórias dos melhores resultados a partir do método da microdiluição. Os extratos apresentaram melhor atividade para S. aureus (P. amarus com MIC 0,16 mg/mL e P. tenellus com MIC  0,10 mg/mL), frente a B. subtilis os resultados também foram satisfatórios (P. amarus 0,25mg/mL e P. tenellus 0,30mg/mL). A tintura comercial apresentou MIC maior que 1mg/mL apenas para S. aureus com MIC de 0,70mg/mL, já para B. subtilis apresentou MIC 1,10mg/mL. Com relação as leveduras, todas as substâncias resultaram ser menos expressivos  que para as bactérias gram-positivas, variando de 0,70 a 1mg/mL para os extratos brutos e 1,10 a 1,20 mg/mL para a tintura comercial. Conclusão: este estudo mostrou o potencial antibacteriano e antifúngicos destas espécies cujo valor terapêutico pode ser melhor avaliado. Descritores: Phyllanthus, CMI, antimicrobiana, fungicida. RESUMEN Objetivo: determinar la actividad antimicrobiana de tres especies de plantas del género Phyllanthus (P. amarus Schum y Thom., P. tenellus Roxb. y Phyllanthus niruri  L.) y su tinte comercial (phytotherapic), frente a las bacterias y levaduras. Métodos: se evaluó la actividad antimicrobiana contra microorganismos por el método de difusión en disco y, a continuación, detectado concentración mínima inhibitoria de los mejores resultados de la microdilución método. Resultados: los medicamentos especies actividad antimicrobiana de las bacterias Gram-positivas. El tinte comercial mostraron un importante actividad antimicrobiana. Los extractos mostraron la mejor actividad para S. aureus (P. amarus MIC con 0,16 mg / ml y P. tenellus MIC con 0,10 mg / ml), frente a B. subtilis resultados también fueron satisfactorios (P. amarus 0,25 mg / ml y P. tenellus 0,30 mg / ml). El tinte comercial mostró superior a 1mg/ml MIC sólo para S. aureus con CMI de 0,70 mg / ml, y para B. subtilis mostró MIC 1,10 mg / mL. Con respecto a la levadura, todas las sustancias eran menos importantes que para las bacterias gram-positivas, que van desde 0,70 a 1mg/ml de extractos crudos y de 1,10 a 1,20 mg / ml de tintura para el comercio. Conclusión: este estudio demostró el potencial antibacteriano y antimicótico valor terapéutico de estas especies pueden ser mejor evaluadas. Descriptores: Phyllanthus, CMI, los antibióticos, fungicidas. 

scholarly journals Synthesis and Antimicrobial Activity of Bis-4,6-sulfonamidated 5,7-Dinitrobenzofuroxans

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Irina V. Galkina ◽  
Elena V. Tudriy ◽  
Yuliya V. Bakhtiyarova ◽  
Luiza M. Usupova ◽  
Marina P. Shulaeva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Fungus ◽  
Fungal Strain ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The Stability

A new series of bis-4,6-sulfonamidated 5,7-dinitrbenzofuroxans  7–11had been synthesized and tested for antimicrobial activity. The structures of new sulfanilamide derivatives were characterized by elemental analysis, IR spectroscopy, and mass spectrometry (MALDITOF). The synthesized compounds were tested for theirin vitroantimicrobial activity using the disk diffusion method against Gram-positive bacteriaStaphylococcus aureus; the Gram-negative bacteriaEscherichia coli, Pseudomonas aeruginosa, andProteus mirabilis; the fungal strainAspergillus niger; and the yeast-like pathogenic fungusCandida albicans. Our results indicate that the compounds7–11exhibit potent antimicrobial activity. The stability of the compounds was evaluated by TG and DSC methods.

scholarly journals Evaluation of 1,2-Benzothiazine 1,1-Dioxide Derivatives In Vitro Activity towards Clinical-Relevant Microorganisms and Fibroblasts

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3503 ◽  
Author(s):  
Ruth K. Dudek-Wicher ◽  
Berenika M. Szczęśniak-Sięga ◽  
Rafał J. Wiglusz ◽  
Jan Janczak ◽  
Marzenna Bartoszewicz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Local Treatment ◽  
Fibroblast Cell ◽  
Microbial Pathogens ◽  
Diffusion Method ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Microdilution Method

The global concern related with growing number of bacterial pathogens, resistant to numerous antibiotics, prone scientific environment to search for new antimicrobials. Antiseptics appear to be suitable candidates as adjunctive agents to antibiotics or alternative local treatment option aiming to prevent and treat infections. 1,2-benzothiazines are considered one the most promising of them. In this research twenty 1,2-benzothiazine 1,1-dioxide derivatives were scrutinized with regard to their biological activity. Three of them are new. For evaluation of compounds’ activity against microbial pathogens, disk diffusion method and serial microdilution method was applied. To establish the cytotoxicity profile of tested 1,2-benzothiazines 1,1-dioxides derivatives, the cytotoxicity assay using fibroblasts L292 was performed. Antimicrobial activity of all tested compounds against Gram-positive Staphylococcus aureus and Enterococcus faecalis strains was higher than antimicrobial activity of DMSO solvent, which possesses antimicrobial activity itself. Gram-negative P. aeruginosa, E. coli and K. pneumoniae have shown susceptibility only to compounds 3e, 7i and 7l. None of tested compounds was effective against C. albicans. Compound 6g has demonstrated the strongest antimicrobial potency (MIC = 0.00975 mg/mL) among compounds of series 6. Compounds of series 7, namely 7d, 7f, 7g had the lowest minimum inhibitory concentration (MIC). Compound 7f displayed also the lowest cytotoxic effect against fibroblast cell line among series 7 compounds. All tested derivatives displayed lower MIC against Gram-positive bacteria than commercially applied antiseptic, povidone iodine, which MIC value range for tested Gram-positive bacteria was 1.56–6.25 mg/mL.

scholarly journals Antimicrobial Activity of Toothpastes Containing Natural Extracts, Chlorhexidine or Triclosan

2014 ◽  
Vol 25 (3) ◽  
pp. 186-190 ◽  
Author(s):  
Andiara De Rossi ◽  
Danielly Cunha Araújo Ferreira ◽  
Raquel Assed Bezerra da Silva ◽  
Alexandra Mussolino de Queiroz ◽  
Léa Assed Bezerra da Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Culture Media ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Natural Extracts

Abstract: The objective of this in vitro study was to evaluate the antimicrobial effect of toothpastes containing natural extracts, chlorhexidine or triclosan. The effectiveness of toothpastes containing natural extracts (Parodontax(r)), 0.12% chlorhexidine (Cariax(r)), 0.3% triclosan (Sanogil(r)) or fluoride (Sorriso(r), control) was evaluated against yeasts, Gram-positive and Gram-negative bacteria using the disk diffusion method. Water was used as a control. Disks impregnated with the toothpastes were placed in Petri dishes containing culture media inoculated with 23 indicative microorganisms by the pour plate method. After incubation, the inhibition growth halos were measured and statistical analyses (α=0.05) were performed. The results indicated that all formulations, except for conventional toothpaste (Sorriso(r)), showed antimicrobial activity against Gram-positive bacteria and yeasts. The toothpaste containing natural extracts (Parodontax(r)) was the only product able to inhibit the growth of Pseudomonas aeruginosa. The toothpastes containing chlorhexidine, triclosan or natural extracts presented antimicrobial activity against Gram-positive bacteria and yeasts.

scholarly journals Antimicrobial activity of leaf and flower extracts of Lippia Nodiflora L. (Verbenacea)

2012 ◽  
Vol 52 (4) ◽  
pp. 401-403 ◽  
Author(s):  
Zahra Zare ◽  
Ahmed Majd ◽  
Taher Nejad Sattari ◽  
Alireza Iranbakhsh ◽  
Sedigue Mehrabian
Keyword(s):  
Antimicrobial Activity ◽  
Micrococcus Luteus ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Esherichia Coli

Abstract Antimicrobial activities of the methanolic extracts from the leaves and flowers of Lippia nodiflora L. (Verbenaceae), were studied by the disk diffusion method. The extracts showed antimicrobial impact on bacteria such as Bacillus subtilis, B. cereus, Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, K. oxytoca and Esherichia coli as well as fungi such Aspergillus niger and Candida albicanse. The results showed that increasing concentrations of extracts increased the antimicrobial activities in all of the microorganisms. Bacteria were more sensitive than fungi, and gram positive bacteria were more sensitive than gram negative ones.

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