scholarly journals The Valorization of Spent Coffee Grounds Extract as A Prospective Insecticidal Agent Against Some Main Key Pests of Phesolus Vulgaris in Laboratory and Field

2021 ◽  
Author(s):  
Hany Hussein ◽  
Waleed Abouamer ◽  
Hatem Ali ◽  
Manal El-khadragy ◽  
Hany Yehia ◽  
...  
Keyword(s):  
High Performance ◽  
Social Economic ◽  
Methanolic Extract ◽  
Spent Coffee Grounds ◽  
Insecticidal Effect ◽  
Starting Point ◽  
Coffee Grounds ◽  
Acetylcholinesterase Enzyme ◽  
And Control ◽  
Contact Bioassay

Abstract Exploiting massive amounts of food and agro-waste represents a severe social, economic, and environmental issue. Under the growing demand toward food products free of toxic synthetic insecticides, the methanolic extract of spent coffee grounds (SCGs), which represent the main by-product of coffee production, was applied in the current study as a bioinsecticide against the main pests of the green bean; Spodoptera littoralis, Agrotis ipsilon, Bemisia tabaci, Empoasca fabi and Aphis craccivora. Deterrent assay, contact bioassay, and lethal concentration analysis were performed to reveal the repellent, antifeedant, and oviposition deterrent effects. Parallel to the above-performed bioassays, the phytochemical composition of the SCGs methanolic extract was investigated via High-Performance Liquid Chromatography (HPLC) analysis. Fourteen phenolic acids and five flavonoids in addition to caffeine (alkaloid) have been identified in the extract. Cinnamic, rosmarinic, and gallic acids were the predominant phenolics, while apigenin-7-glucoside was the main flavonoid, followed by naringin, catechin, and epicatechin. The extract of SCGs showed an insecticidal effect, with mortality between 27.5-76% compared to the control (7.4%) and based on the concentration of the extract used. In the same trend, oviposition efficiency revealed different laid egg batches (0.67, 2.33, 7.33, and 8.67 batch/jar) for 100, 50, 25% of the SCGs extract and control. Finally, the major components of SCGs extract were docked into insecticide acetylcholinesterase enzyme to explore their potential of inhibition, where apigenin-7-glucoside showed a higher binding affinity, followed by catechin, compared to the control (lannate). Obtained findings could be a starting point to develop novel bioinsecticides from SCGs.

scholarly journals Improving the Recovery of Phenolic Compounds from Spent Coffee Grounds (SCG) by Environmentally Friendly Extraction Techniques

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 613
Author(s):  
Ilhami Okur ◽  
Betul Soyler ◽  
Purlen Sezer ◽  
Mecit Halil Oztop ◽  
Hami Alpas
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Morphological Changes ◽  
Total Phenolic ◽  
Extraction Techniques ◽  
Spent Coffee Grounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Coffee Grounds

The aim of this study was to investigate and compare the effects of different extraction techniques (high hydrostatic pressure-assisted extraction (HHPE), ultrasound-assisted extraction (UAE), and classical solvent extraction (CSE)) on phenolic compounds from spent coffee grounds (SCG). Different HHPE parameters (300, 400 and 500 MPa at 25 °C for 5, 10 and 15 min) and UAE parameters (40%, 50%, and 60% amplitude at 25 °C for 5, 10 and 15 min) were used. These techniques were compared with CSE (at 50 °C for 30 min) according to total phenolic content (TPC), antioxidant activity (AA), high-performance liquid chromatography (HPLC), scanning electron microscopy (SEM), and infrared (IR) spectroscopy. The results showed that eco-friendly techniques increased the TPC and AA compared to CSE and morphological changes were verified by SEM results. Furthermore, chlorogenic and caffeic acid were also quantified by using HPLC. Chlorogenic acid was found as the main phenolic compound in spent coffee grounds (SCG). The highest chlorogenic acid was detected as 85.0 ± 0.6 mg/kg FW with UAE at 60% amplitude for 15 min. In brief, for the extraction of phenolic compounds from waste SCG eco-friendly techniques such as HHPE and/or UAE were more convenient than CSE.

scholarly journals Determination of Caffeic Acid in Ethanolic Extract of Spent Coffee Grounds by High-Performance Liquid Chromatography with UV Detection

2021 ◽  
Vol 21 (5) ◽  
pp. 1281
Author(s):  
Michael Raharja Gani ◽  
Enade Perdana Istyastono
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Caffeic Acid ◽  
High Performance ◽  
Spent Coffee Grounds ◽  
Uv Detector ◽  
Column Temperature ◽  
Coffee Grounds ◽  
Spent Coffee Ground ◽  
Relative Standard

We developed a method for determining the caffeic acid in spent coffee grounds. The spent coffee ground solution was prepared by blending 3 g spent coffee grounds with 60 mL ethanol/water (40/60 v/v) for 2 h on a hot plate magnetic stirrer (60 °C, 350 rpm). The mixture was filtered and the filtrate was concentrated under vacuum (60 °C) to 5 mL. The method employed a reversed-phase high-performance liquid chromatography with a UV detector. We used a Phenomenex Luna column (250 × 4.6 mm; i.d., 5 µm) under isocratic elution, and the mobile phase was acetonitrile-methanol-aqueous formic acid (10:10:80 v/v), with a flow rate of 0.9 mL/min. Analysis was performed at 324 nm. The column temperature was set at 27 °C temperature. The results showed that this method was selective for quantifying the caffeic acid in spent coffee grounds with good linearity in the range of 1.31–17.07 μg/mL. The detection and quantitation limits were 0.28 and 0.84 μg/mL, respectively. The mean intraday and interday recoveries were 83.80–95.17% and 82.16–97.40%, respectively. Intraday and interday precision expressed as the relative standard deviation (RSD) were below 7.3%. There was 0.17% ± 0.006 w/w caffeic acid in the spent coffee grounds (RSD = 3.63%, n = 3).

scholarly journals HPLC-Based Chemometric Analysis for Coffee Adulteration

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 880
Author(s):  
Wai Lok Cheah ◽  
Mingchih Fang
Keyword(s):  
High Performance ◽  
Direct Injection ◽  
Principal Component ◽  
Chemometric Analysis ◽  
Coffee Bean ◽  
Spent Coffee Grounds ◽  
Targeted Analysis ◽  
Coffee Beans ◽  
Coffee Grounds ◽  
Spent Coffee Ground

Coffee is one of the top ten most adulterated foods. Coffee adulterations are mainly performed by mixing other low-value materials into coffee beans after roasting and grinding, such as spent coffee grounds, maize, soybeans and other grain products. The detection of adulterated coffee by high performance liquid chromatography (HPLC) is recognized as a targeted analytical method, which carbohydrates and other phenolic compounds are usually used as markers. However, the accurate qualitation and quantitation of HPLC analyses are time consuming. This study developed a chemometric analysis or called non-targeted analysis for coffee adulteration. The HPLC chromatograms were obtained by direct injection of liquid coffee into HPLC without sample preparation and the identification of target analytes. The distinction between coffee and adulterated coffee was achieved by statistical method. The HPLC-based chemometric provided more characteristic information (separated compounds) compared to photospectroscopy chemometric which only provide information of functional groups. In this study, green Arabica coffee beans, soybeans and green mung beans were roasted in industrial coffee bean roaster and then ground. Spent coffee ground was dried. Coffee and adulterants were mixed at different ratio before conducting HPLC analysis. Principal component analysis (PCA) toward HPLC data (retention time and peak intensity) was able to separate coffee from adulterated coffee. The detection limit of this method was 5%. Two models were built based on PCA data as well. The first model was used to differentiate coffee sample from adulterated coffee. The second model was designed to identify the specific adulterants mixed in the adulterated coffee. Various parameters such as sensitivity (SE), specificity (SP), reliability rate (RLR), positive likelihood (+LR) and negative likelihood (−LR) were applied to evaluate the performances of the designed models. The results showed that PCA-based models were able to discriminate pure coffee from adulterated sample (coffee beans adulterated with 5%–60% of soybeans, green mung beans or spent coffee grounds). The SE, SP, RLR, +LR and −LR for the first model were 0.875, 0.938, 0.813, 14.1 and 0.133, respectively. In the second model, it can correctly distinguish the adulterated coffee from the pure coffee. However, it had only about a 30% chance to correctly determine the specific adulterant out of three designed adulterants mixed into coffee. The SE, RLR and −LR were 0.333, 0.333 and 0.667, respectively, for the second model. Therefore, HPLC-based chemometric analysis was able to detect coffee adulteration. It was very reliable on the discrimination of coffee from adulterated coffee. However, it may need more work to tell discern which kind adulterant in the adulterated coffee.

Phytoceuticals in aniseeds: a practical approach for isolation and identification

2016 ◽  
Vol 5 (01) ◽  
pp. 4701
Author(s):  
Shobha R. I. ◽  
Andallu B.
Keyword(s):  
Chronic Diseases ◽  
Column Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Analytical Techniques ◽  
Therapeutic Agents ◽  
Systematic Research ◽  
Isolation And Identification ◽  
Traditional System ◽  
Pharmacologically Active

Nature has provided mankind with a broad and structurally diverse array of pharmacologically active chemical compounds, phytoceuticals, which have proved to be indispensable for the cure of chronic diseases or as lead structures for novel therapeutic agents. Almost, 70% of modern medicines in India have been developed from plants used in the traditional system of medicine. With the technological advances and the development of more sophisticated isolation and analytical techniques, there is great scope for further systematic research to screen and isolate many more phytoceuticals which might be more effective/as effective as synthetic drugs and thereby assess their potential in protecting against chronic diseases. The present investigation is aimed at isolation and identification of phytoceuticals in a spice, aniseed (Pimpinella anisum L), therapeutically less-exploited and widely used only for culinary purpose, using analytical techniques viz. column chromatography, high performance thin layer chromatography (HPTLC.) and nuclear magnetic resonance spectrometry. Among all the solvent fractions of methanolic extract tested, methanolic extract and ethyl acetate fraction possessed highest amounts of bioactive compounds viz. phenolics, flavonoids, flavonols which was confirmed by qualitative, quantitative and HPTLC. analyses. Spectral analysis using NMR. of one of the sub-fractions of aniseeds obtained by column chromatography, revealed the presence of a glycosylated flavone, luteolin-6C-glucoside in aniseeds.  The present study revealed aniseeds to be a great source of bioactive phytoceuticals which can be novel candidates for development of new therapeutic agents.

scholarly journals Reduction of Polycyclic Aromatic Hydrocarbons (PAHs) in Sesame Oil Using Cellulosic Aerogel

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 644
Author(s):  
Do-Yeong Kim ◽  
Boram Kim ◽  
Han-Seung Shin
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Oil Quality ◽  
Acid Value ◽  
Quality Parameters ◽  
Sesame Oil ◽  
Miscanthus Sinensis ◽  
Polycyclic Aromatic ◽  
And Control

The effect of cellulosic aerogel treatments used for adsorption of four polycyclic aromatic hydrocarbons (PAHs)—benzo[a]anthracene, chrysene, benzo[b]fluoranthene, and benzo[a]pyrene [BaP])—generated during the manufacture of sesame oil was evaluated. In this study, eulalia (Miscanthus sinensis var. purpurascens)-based cellulosic aerogel (adsorbent) was prepared and used high performance liquid chromatography with fluorescence detection for determination of PAHs in sesame oil. In addition, changes in the sesame oil quality parameters (acid value, peroxide value, color, and fatty acid composition) following cellulosic aerogel treatment were also evaluated. The four PAHs and their total levels decreased in sesame oil samples roasted under different conditions (p < 0.05) following treatment with cellulosic aerogel. In particular, highly carcinogenic BaP was not detected after treatment with cellulosic aerogel. Moreover, there were no noticeable quality changes in the quality parameters between treated and control samples. It was concluded that eulalia-based cellulosic aerogel proved suitable for the reduction of PAHs from sesame oil and can be used as an eco-friendly adsorbent.

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